scholarly journals Insecticide Resistance Testing of Culex quinquefasciatus and Aedes albopictus from Mississippi

2019 ◽  
Vol 35 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Sarah J. McInnis ◽  
Jerome Goddard ◽  
J. Hunter Deerman ◽  
Tina Nations ◽  
Wendy C. Varnado
Keyword(s):  
Insecticide Resistance ◽  
Aedes Albopictus ◽  
Culex Quinquefasciatus ◽  
Resistance Testing

scholarly journals High insecticide resistance mediated by different mechanisms in Culex quinquefasciatus populations from the city of Yaoundé, Cameroon

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abdou Talipouo ◽  
Konstantinos Mavridis ◽  
Elysée Nchoutpouen ◽  
Borel Djiappi-Tchamen ◽  
Emmanouil Alexandros Fotakis ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
High Resistance ◽  
Culex Quinquefasciatus ◽  
Control Strategies ◽  
Cuticular Hydrocarbon ◽  
Control Measures ◽  
High Frequencies ◽  
The City ◽  
Integrated Vector Control

AbstractCulex mosquitoes particularly Culex quinquefasciatus are important arboviral and filariasis vectors, however despite this important epidemiological role, there is still a paucity of data on their bionomics. The present study was undertaken to assess the insecticide resistance status of Cx. quinquefasciatus populations from four districts of Yaoundé (Cameroon). All Culex quinquefasciatus populations except one displayed high resistance to bendiocarb and malathion with mortalities ranging from 0 to 89% while high resistance intensity against both permethrin and deltamethrin was recorded. Molecular analyses revealed high frequencies of the ACE-1 G119S mutation (ranging from 0 to 33%) and kdr L1014F allele (ranging from 55 to 74%) in all Cx. quinquefasciatus populations. Significant overexpression was detected for cytochrome P450s genes CYP6AA7 and CYP6Z10, as well as for Esterase A and Esterase B genes. The total cuticular hydrocarbon content, a proxy of cuticular resistance, was significantly increased (compared to the S-lab strain) in one population. The study confirms strong insecticide resistance mediated by different mechanisms in Cx. quinquefasciatus populations from the city of Yaoundé. The expansion of insecticide resistance in Culex populations could affect the effectiveness of current vector control measures and stress the need for the implementation of integrated vector control strategies in urban settings.

Organophosphorus insecticide resistance in a new strain of Culex quinquefasciatus (Diptera: Culicidae) from Tanga, Tanzania

1993 ◽  
Vol 83 (1) ◽  
pp. 67-74 ◽  
Author(s):  
A. Khayrandish ◽  
R.J. Wood
Keyword(s):  
Insecticide Resistance ◽  
Culex Quinquefasciatus ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Laboratory Culture ◽  
Fourth Instar ◽  
Resistance Allele ◽  
Susceptible Control ◽  
The Mean

AbstractFourth instar larvae of a new strain of Culex quinquefasciatus Say from Tanzania (TANGA) were tested for insecticide resistance. Initially, the resistance ratio (RR) to chlorpyrifos was 41.8, to temephos 30.8, to propoxur 3.7. After 2–3 years of laboratory culture, resistance to chlorpyrifos and propoxur had declined (chlorpyrifos 5.7, 3.8; propoxur 1.9, permethrin 1.9). Significant synergism was found between s, s, s-tributyl trithiophosphate (DEF) and chlorphyrifos, reducing the RR from 8.0 to 2.5. Synergism between piperonyl butoxide and permethrin was less than in a susceptible control strain. Twelve esterase isozymes of different relative mobilities (Rm) on polyacrylamide gel electrophoresis were identified, ten of which remained when the strain was reinvestigated two years (approximately 32 generations) later. Null activity for all but one of these bands was observed in some larvae. Four esterase bands (Rm 0.25, 0.27, 0.31, 0.34, designated A2, A3, B2, B3) showed polymorphism in activity, with very intense bands in some larvae. The mean frequency of bands with activity greater than standard, declined as organophosphorus (OP) resistance declined, but resistance was unconnected with the frequency of nulls at these positions. In mass larval assays of in vitro sensitivity of acetylcholinesterase (AChE) to propoxur, the I50 exceeded 10x10−4M, compared with 0.1x10−4M in a reverted resistant strain (RANGOON). Single larvel assays revealed heterogeneity, which was interpreted on the basis of an AChE resistance allele (AceR) with a frequency of 0.23.

scholarly journals Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Sofia Balaska ◽  
Emmanouil A. Fotakis ◽  
Ilias Kioulos ◽  
Linda Grigoraki ◽  
Spyridoula Mpellou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Evidence Based ◽  
Molecular Monitoring ◽  
Support Evidence
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