scholarly journals Plasmodium Infections in Anopheles Mosquitoes in Ubon Ratchathani Province, Northeastern Thailand During a Malaria Outbreak

2018 ◽  
Vol 34 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Anchana Sumarnrote ◽  
Vincent Corbel ◽  
Hans J. Overgaard ◽  
Olivier Celhay ◽  
Nattapol Marasri ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Malaria Vectors ◽  
Anopheles Species ◽  
Community Activities ◽  
Anopheles Mosquitoes ◽  
Human Landing ◽  
Suitable Habitats ◽  
Malaria Outbreak ◽  
Northeastern Thailand

ABSTRACT An unprecedented malaria outbreak occurred in Ubon Ratchathani Province, northeastern Thailand, in 2014. The province showed the highest number of malaria cases of all Thai provinces. Five entomological surveys were conducted at 8 sentinel sites from September 2013 to September 2015 to address the role of different Anopheles species in malaria transmission. Mosquito collections were conducted using human landing catches and cow bait. A total of 10,369 Anopheles mosquitoes were collected and 2,240 were morphologically identified as potential malaria vectors, including An. dirus (n = 78), An. minimus (n = 18), An. sawadwongporni (n = 4), An. barbirostris s.l. (n = 819), An. philippinensis (n = 612), An. nivipes (n = 676), An. annularis (n = 42), An. aconitus (n = 7), and An. rampae (n = 142). Real-time polymerase chain reaction was used to screen for the presence of Plasmodium spp. in salivary glands. The proportion of primary vectors of surveyed villages was very low (<1%), and no Plasmodium-infected specimens were detected among in the 2,240 Anopheles mosquitoes tested. The absence of positive Plasmodium samples during malaria outbreaks suggests that malaria transmission most likely occurred outside the villages, particularly in the deep-forested hilly areas that provided suitable habitats for competent malaria vectors. These results emphasize the need to develop vector control related to village community activities to reduce malaria transmission along Thailand border areas.

scholarly journals Species Composition and Role of Anopheles Mosquitoes in Malaria Transmission Along Badagry Axis of Lagos Lagoon, Lagos, Nigeria

2010 ◽  
Vol 2 ◽  
pp. IJIS.S4698 ◽  
Author(s):  
I.O. Oyewole ◽  
C.A. Ibidapo ◽  
O.O. Okwa ◽  
A.O. Oduola ◽  
G.O. Adeoye ◽  
...  
Keyword(s):  
Species Composition ◽  
Malaria Transmission ◽  
Control Programme ◽  
Anopheles Mosquitoes ◽  
Vector Control Programme ◽  
Baseline Information ◽  
Falciparum Sporozoite ◽  
Lagos Lagoon ◽  
Sporozoite Infection

Three communities along Badagry axis of the Lagos lagoon were sampled for indoor resting Anopheles mosquitoes in order to determine their species composition, relative abundance, density and contribution to malaria transmission in the coastal ecosystem. A total of 1938 adult female Anopheles mosquitoes collected from 2005 to 2007 constituted three species viz Anopheles gambiae, An melas and An. nili. The Polymerase Chain Reaction (PCR)–-based tests indicated that more than three-fourth of the An. gambiae s.l (75.8%) population belongs to An. gambiae s.s the remaining were An. melas. Further analysis showed that all the An. gambiae s.s was the M form. ELISA-based analyses indicated that An. gambiae s.s and An. melas were the main vectors of malaria in this area with an overall P. falciparum sporozoite infection rate of 4.8% and 6.5% respectively. Both species also maintained relatively high EIR indicating their prominent roles in malaria transmission in the study area. All the An. nili tested were negative for P. falciparum sporozoite infection. This study provides baseline information for planning vector control programme relevant to reduction of malaria transmission in the coastal areas of Nigeria.

scholarly journals Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Anchana Sumarnrote ◽  
Hans J. Overgaard ◽  
Vincent Corbel ◽  
Kanutcharee Thanispong ◽  
Theeraphap Chareonviriyaphap ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Control Strategies ◽  
Resistance Mechanism ◽  
Abundant Species ◽  
Malaria Vectors ◽  
Pcr Assay ◽  
Insecticide Susceptibility ◽  
Human Landing ◽  
Early Peak

Abstract Background Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where increasing numbers of malaria cases were reported in 2014. Methods Between 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. Anopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. Synergist bioassays were carried out with An. hyrcanus (s.l.) due to their resistance to all insecticides. An ITS2-PCR assay was conducted to identify to species the Hyrcanus group specimens. Results Out of 10,361 Anopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the Hyrcanus Group (subgenus Anopheles), followed by An. barbirostris group (7.9%), An. nivipes (6.5%), An. philippinensis (5.9%) and the other 14 Anopheles species. Specimens of the Hyrcanus Group were more prevalent during the rainy season and were found to be highly zoophilic. Anopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. ITS2-PCR assay conducted on 603 DNA samples from specimens within the Hyrcanus Group showed the presence of five sisters species. Anopheles peditaeniatus was the most abundant species (90.5%, n = 546), followed by An. nitidus (4.5%, n = 27), An. nigerrimus (4.3%, n = 26), An. argyropus (0.5%, n = 3), and An. sinensis (0.2%, n = 1). All An. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and DDT 4% and synergist tests) belonged to An. peditaeniatus. The degree of resistance in An. peditaeniatus to each of these three insecticides was approximately 50%. Addition of PBO (Piperonyl butoxide), but not DEF (S.S.S-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism. Conclusions A better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies.

scholarly journals Plasmodium evasion of mosquito immunity and global malaria transmission: The lock-and-key theory

2015 ◽  
Vol 112 (49) ◽  
pp. 15178-15183 ◽  
Author(s):  
Alvaro Molina-Cruz ◽  
Gaspar E. Canepa ◽  
Nitin Kamath ◽  
Noelle V. Pavlovic ◽  
Jianbing Mu ◽  
...  
Keyword(s):  
Immune System ◽  
Malaria Transmission ◽  
Immune Evasion ◽  
Mosquito Species ◽  
Haplotype Diversity ◽  
Malaria Vectors ◽  
Selective Force ◽  
Genetic Traits ◽  
Geographic Population

Plasmodium falciparum malaria originated in Africa and became global as humans migrated to other continents. During this journey, parasites encountered new mosquito species, some of them evolutionarily distant from African vectors. We have previously shown that the Pfs47 protein allows the parasite to evade the mosquito immune system of Anopheles gambiae mosquitoes. Here, we investigated the role of Pfs47-mediated immune evasion in the adaptation of P. falciparum to evolutionarily distant mosquito species. We found that P. falciparum isolates from Africa, Asia, or the Americas have low compatibility to malaria vectors from a different continent, an effect that is mediated by the mosquito immune system. We identified 42 different haplotypes of Pfs47 that have a strong geographic population structure and much lower haplotype diversity outside Africa. Replacement of the Pfs47 haplotypes in a P. falciparum isolate is sufficient to make it compatible to a different mosquito species. Those parasites that express a Pfs47 haplotype compatible with a given vector evade antiplasmodial immunity and survive. We propose that Pfs47-mediated immune evasion has been critical for the globalization of P. falciparum malaria as parasites adapted to new vector species. Our findings predict that this ongoing selective force by the mosquito immune system could influence the dispersal of Plasmodium genetic traits and point to Pfs47 as a potential target to block malaria transmission. A new model, the “lock-and-key theory” of P. falciparum globalization, is proposed, and its implications are discussed.

scholarly journals Host-seeking behavior and hourly biting rhythm of vectors in malaria endemic district of Arba Minch area, Southwest Ethiopia

2020 ◽  
Author(s):  
Melkam Abiye Zeru ◽  
Simon Shibru ◽  
Fekadu Massebo
Keyword(s):  
Malaria Vector ◽  
Negative Binomial ◽  
Malaria Vectors ◽  
Malaria Vector Control ◽  
Southwest Ethiopia ◽  
Anopheles Mosquitoes ◽  
Host Seeking ◽  
Human Landing ◽  
Seeking Behavior ◽  
The Impact

Abstract Background The early and outdoor biting behaviors of malaria vectors are among the key challenges in malaria control. Hence, understanding the host-seeking behavior and the peak biting time of malaria vectors is important in malaria vector control programs. This study assessed the host-seeking behavior and hourly biting activity of malaria mosquitoes in Chano Mille village in Arba Minch district, southwest Ethiopia. Methods The first trial was done by keeping cattle together with human that collects the entered mosquitoes in tent, while the other was done by keeping cattle outside a 1 m distance from human collector inside a tent. In both trials, mosquito collation was done inside tents baited by cattle and human using human landing catches (HLC) techniques. Four human volunteers’ were recruited and trained to collect mosquitoes in the four tents from 18:00-24:00 for three months. Two tents were selected randomly for human alone and two for cattle baited collections in the first night and then rotated to minimize the variation due to location of tents and collectors skill. The tent trial was done close to the shore of the Lake Abaya to minimize the interference of other animals on mosquito movement. The peak biting hour of malaria vectors was assessed within a Chano village from 18:00-6:00. Mosquito collation was done both indoor and outdoor by HLC. Morphological speciation of Anopheles mosquitoes was done. The sporozoite infectivity status of Anopheles pharoensis was examined using enzyme-linked immuno-sorbent assay technique. The data was analyzed using a Generalized Estimating Equations with a negative binomial distribution. Results An. pharoensis, An. gambiae complex and An. tenebrosus were the three species documented during the trial. Keeping cattle together with human collector inside the tent attracted 42% ( P < 0.001) more An. pharoensis compared to human alone tent. Also, keeping cattle outside near to a tent with human at 1 m distance, attracted the entering An. pharoensis into the tent inside with human by 46% ( P = 0.002) than human in a tent with no cattle outside the same tent. The impact was not significant for An. gambiae complex and An. tenebrosus. Anopheles pharoensis and An. gambiae complex showed early night biting activity with peak biting from 19.00-20:00 which was significant for both An. gambiae complex ( P < 0.001) and An. pharoensis ( P = 0.015). Anopheles gambiae complex was mainly biting humans outdoor in the village. Conclusions Finally, keeping cattle within and close to human dwellings could increase malaria vectors bite exposure particularly to the zoophilic malaria vector An. pharoensis and, hence deployment of cattle far from human residence could be recommended to reduce the human exposure. The outdoor and early hours biting behavior of the An. gambiae complex could be a threat for success of current indoor based interventions and hence, tools could be designed to reduce this threat.

scholarly journals New insights into malaria vector bionomics in Lao PDR: a nationwide entomology survey

2020 ◽  
Author(s):  
Sebastien Marcombe ◽  
Santi Maithaviphet ◽  
Julie Bobichon ◽  
Nothasin Phommavan ◽  
Simone Nambanya ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Sibling Species ◽  
Rural Areas ◽  
Lao Pdr ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Morphological Identification ◽  
Anopheles Mosquitoes ◽  
Malaria Burden ◽  
Human Landing

Abstract Background . In Laos, the malaria burden remains high despite a significant reduction of cases during the last decade. In the context of the disease elimination by 2030, a nationwide entomological survey was conducted to better understand the distribution, abundance and behavior of major malaria vectors ( Anopheles spp.) in the country. Methods . Mosquito collections were implemented in ten villages from ten provinces during the rainy and dry seasons of 2014 and 2015 by using human landing catch (HLC) and cow bait collection (CBC) methods. After morphological identification in the field, molecular identification of the sibling species of Anopheles mosquitoes from the Funestus, Leucosphyrus, and Maculatus groups were determined using PCR specific alleles. A screening of Plasmodium falciparum and P. vivax infections in the vectors was carried out by quantitative PCR assays. Results. A total of 14,146 adult mosquitoes representing 25 different Anopheles species were collected and morphologically identified. Molecular identification revealed the presence of 12 sibling species within the main primary vector groups including An. maculatus s.s., An. rampae , An. sawadwongporni , An. pseudowillmori , An. dravidicus , An. minimus s.s., An. aconitus , An. pampanai , An. harrisoni , An. dirus s.s., An. baimaii , An. nemophilous . Anopheles maculatus and An. minimus were predominant during both the dry and rainy seasons, but showed highly zoophilic preferences (Zoophilic index of 98% and 95%, respectively). Overall, 22% of the total malaria vectors were collected between 10:00 PM and 5:00 AM indoors when people are sleeping. Twenty-seven percent of primary and secondary vectors were collected outdoors before 10:00 PM or after 5:00 AM, times when people are usually awake and outdoors. Only two specimens were positive for P. falciparum , one An. aconitus from Phongsaly and one An. minimus from Vientiane Province Conclusions. The results indicate that people living in rural areas in Laos are constantly exposed to malaria vectors throughout the year and specifically outdoors. The use of LLINs/IRS remains important but innovative tools and new strategies are needed to address locally, the early and outdoor malaria transmission. Lack of expertise in general entomological methods may further exacerbate the situation.

scholarly journals Impact of sugarcane irrigation on malaria vector Anopheles mosquito fauna, abundance and seasonality in Arjo-Didessa, Ethiopia

2020 ◽  
Author(s):  
Assalif Demissew Shifera ◽  
Dawit Hawaria ◽  
Solomon Kibret ◽  
Abebe Animut ◽  
Arega Tsegaye ◽  
...  
Keyword(s):  
Species Composition ◽  
Malaria Vector ◽  
Mosquito Species ◽  
Malaria Vectors ◽  
Anopheline Mosquito ◽  
Chi Square ◽  
Anopheles Species ◽  
Mosquito Abundance ◽  
Anopheles Mosquitoes ◽  
The Impact

Abstract Background: Although irrigation activities are increasing in Ethiopia, limited studies evaluated their impact on malaria vector mosquito composition, abundance and seasonality. This study aimed at evaluating the impact of sugarcane irrigation on species composition, abundance and seasonality of malaria vectors. Methods : Adult Anopheles mosquitoes were collected using CDC light traps from three irrigated and three non-irrigated clusters in and around Arjo-Didessa sugarcane irrigation scheme in southwestern Ethiopia. Mosquito collections were conducted in four seasons: two wet and two dry, in 2018 and 2019. Mosquito species composition, abundance and seasonality were compared between irrigated and non-irrigated clusters. Anopheles mosquitoes were identified to species using morphological keys and An. gambiae s.l to sibling species using PCR. Chi-square was used to analyze the association between Anopheles species occurrence and environmental and seasonal parameters. Results: Overall, 2,108 female Anopheles mosquitoes comprising of six species were collected. Of these, 92.7% (n=1954) were from irrigated clusters and 7.3% (n=154) from the non-irrigated. An. gambiae s.l was the most abundant (67.3%) followed by An. coustani complex (25.3 %) and An. pharoensis (5.7%). PCR based identification revealed that 74.7% (n=168) of the An. gambiae s.l were An. arabiensis and 22.7% (n=51) An. amharicus . Density of An. gambiae s.l. (both indoor and outdoor) was higher in irrigated than non-irrigated clusters. The overall anopheline mosquito abundance during the wet seasons (87.2%; n=1837) was higher than the dry seasons (12.8%; n=271). Conclusion : The ongoing sugarcane irrigation activities in Arjo-Didessa created conditions suitable for increased malaria transmitting Anopheles species diversity and abundance. This in turn could drive malaria transmission in Arjo-Didessa and its environs in both dry and wet seasons. Thus, currently practiced malaria vector interventions need to be strengthened and consider larval source management to reduce vector abundance in the irrigated areas. Key Words: Malaria, Irrigation, Anopheles mosquitoes, vector density, An. amharicus , Ethiopia

scholarly journals Malaria in migrant agricultural workers in western Ethiopia: entomological assessment of malaria transmission risk

2021 ◽  
Author(s):  
Sisay Dugassa Lemma ◽  
Mathew Murphy ◽  
Sheleme Chibsa ◽  
Yehualashet Tadesse ◽  
Gedeon Yohannes ◽  
...  
Keyword(s):  
Vector Control ◽  
Malaria Transmission ◽  
Anopheles Arabiensis ◽  
Agricultural Development ◽  
Transmission Risk ◽  
Malaria Vectors ◽  
Residential Housing ◽  
Host Seeking ◽  
Human Landing ◽  
Western Ethiopia

Abstract Background Ethiopia has made great strides in malaria control over the last two decades. However, this progress has not been uniform and one concern has been reported high rates of malaria transmission in large agricultural development areas in western Ethiopia. Improved vector control is one way this transmission might be addressed, but little is known about malaria vectors in this part of the country. Methods To better understand the vector species involved in malaria transmission and their behaviour, human landing collections were conducted in Dangur woreda, Benishangul-Gumuz, between July and December 2017. This period encompasses the months with the highest rain and the peak mosquito population. Mosquitoes were identified to species and tested for the presence of Plasmodium sporozoites. Results The predominant species of the Anopheles collected was Anopheles arabiensis (1,733; i.e. 61.3% of the entire Anopheles), which was also the only species identified with sporozoites (Plasmodium falciparum and Plasmodium vivax). Anopheles arabiensis was collected as early in the evening as 18:00h-19:00h, and host-seeking continued until 5:00h-6:00h. Nearly equal numbers were collected indoors and outdoors. The calculated entomological inoculation rate for An. arabiensis for the study period was 1.41 infectious bites per month. More An. arabiensis were collected inside and outside worker’s shelters than in fields where workers were working at night. Conclusions Anopheles arabiensis is likely to be the primary vector of malaria in the agricultural development areas studied. High rates of human biting took place inside and outdoor near workers’ residential housing. Improved and targeted vector control in this area might considerably reduce malaria transmission.

scholarly journals Malaria in Migrant Agricultural Workers in Dangur District, Benishangul-gumuz, Ethiopia. Entomological Assessment of Malaria Transmission Risk

2020 ◽  
Author(s):  
Sisay Dugassa Lemma ◽  
Mathew Murphy ◽  
Sheleme Chibsa ◽  
Yehualashet Tadesse ◽  
Gedeon Yohannes ◽  
...  
Keyword(s):  
Vector Control ◽  
Malaria Transmission ◽  
Anopheles Arabiensis ◽  
Agricultural Development ◽  
Transmission Risk ◽  
Malaria Vectors ◽  
Residential Housing ◽  
Host Seeking ◽  
Human Landing ◽  
Primary Vector

Abstract Background: Ethiopia has made great strides in malaria control. However, this progress has not been uniform and one concern has been reported high rates of malaria transmission in large agricultural development areas in western Ethiopia. Improved vector control is one way this transmission might be addressed, but little is known about malaria vectors in this part of the country. Methods: To better understand the vector species involved in malaria transmission and their behavior, human landing collections were conducted in Dangur woreda, Benishangul-Gumuz, between July and December 2017. Mosquitoes were identified to species and tested for presence of Plasmodium sporozoites. Results: The predominant species collected was Anopheles arabiensis (61.3% of all Anopheles), which was also the only species identified with sporozoites (P. falciparum and P. vivax). Anopheles arabiensis was collected as early in the evening as 18:00h-19:00h, and host-seeking continued until 5:00h-6:00h. Nearly equal numbers were collected indoors and outdoors. The calculated entomological inoculation rate for An. arabiensis for the study period was 1.41 infectious bites per month. More An. arabiensis were collected inside and outside worker’s shelters than in fields where workers were working at night. Conclusions: An. arabiensis is likely to be the primary vector of malaria in the agricultural development areas studied. High rates of human biting took place inside and outdoor near workers’ residential housing. Improved and targeted vector control in this area might considerably reduce malaria transmission.

scholarly journals Malaria Transmission around the Memve’ele Hydroelectric Dam in South Cameroon: A Combined Retrospective and Prospective Study, 2000–2016

2019 ◽  
Vol 16 (9) ◽  
pp. 1618
Author(s):  
Lili R. Mbakop ◽  
Parfait H. Awono-Ambene ◽  
Stanislas E. Mandeng ◽  
Wolfgang E. Ekoko ◽  
Betrand N. Fesuh ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Malaria Vector ◽  
Disease Transmission ◽  
Disease Surveillance ◽  
Protein Detection ◽  
Malaria Vectors ◽  
Dam Construction ◽  
Vector Species ◽  
Human Landing ◽  
And Control

Dam constructions are considered a great concern for public health. The current study aimed to investigate malaria transmission in the Nyabessan village around the Memve’ele dam in South Cameroon. Adult mosquitoes were captured by human landing catches in Nyabessan before and during dam construction in 2000–2006 and 2014–2016 respectively, as well as in the Olama village, which was selected as a control. Malaria vectors were morphologically identified and analyzed for Plasmodium falciparum circumsporozoite protein detection and molecular identification of Anopheles (A.) gambiae species. Overall, ten malaria vector species were identified among 12,189 Anopheles specimens from Nyabessan (N = 6127) and Olama (N = 6062), including A. gambiae Giles (1902), A. coluzzii Coetzee (2013), A. moucheti Evans (1925), A. ovengensis Awono (2004), A. nili Theobald (1903), A. paludis Theobald (1900), A. zieanni, A. marshallii Theobald (1903), A. coustani Laveran (1900), and A. obscurus Grünberg (1905). In Nyabessan, A. moucheti and A. ovengensis were the main vector species before dam construction (16–50 bites/person/night-b/p/n, 0.26–0.71 infective bites/person/night-ib/p/n) that experienced a reduction of their role in disease transmission in 2016 (3–35 b/p/n, 0–0.5 ib/p/n) (p < 0.005). By contrast, the role of A. gambiae s.l. and A. paludis increased (11–38 b/p/n, 0.75–1.2 ib/p/n) (p < 0.01). In Olama, A. moucheti remained the main malaria vector species throughout the study period (p = 0.5). These findings highlight the need for a strong vector-borne disease surveillance and control system around the Memve’ele dam.

scholarly journals Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting

2020 ◽  
Author(s):  
Denis Escobar ◽  
Krisnaya Ascencio ◽  
Andrés Ortiz ◽  
Adalid Palma ◽  
Gustavo Fontecha
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analyses ◽  
Public Health Problem ◽  
Malaria Vectors ◽  
Morphological Identification ◽  
High Genetic Diversity ◽  
Homogeneous Population ◽  
Anopheles Species ◽  
Anopheles Mosquitoes ◽  
Endemic Areas

Abstract Background : Anopheles mosquitoes are the vectors of malaria, one of the most important infectious diseases in the tropics. More than 500 Anopheles species have been described worldwide, and more than 30 are considered a public health problem. In Honduras, information on the distribution of Anopheles spp. and its genetic diversity is scarce. This study aimed to describe the distribution and genetic diversity of Anopheles mosquitoes in Honduras. Methods : Mosquitoes were captured in 8 locations in 5 malaria endemic departments during 2019. Two collectionmethods were used. Adult anophelines were captured outdoors using CDC light traps and by aspiration of mosquitoes at rest. The morphological identification was performed using taxonomic keys. Genetic analyses included the sequencing of a partial region of the cytochrome oxidase I gene (COI) and the ribosomal internal transcribed spacer 2 (ITS2). Results : A total of 1320 anophelines were collected and identified through morphological keys. Seven Anopheles species were identified. Anopheles albimanus was the most widespread and abundant species (74.02%). To confirm the morphological identification of the specimens, 175 and 122 sequences were obtained for COI and ITS2 respectively. Both markers confirmed the morphological identification. COI showed a greater nucleotide diversity than ITS2 in all species. High genetic diversity was observed within the populations of An. albimanus while An. darlingi proved to be a highly homogeneous population. Phylogenetic analyses revealed clustering patterns in An. darlingi and An. neivai in relation to specimens from South America. New sequences for An. crucians , An. vestitipennis , and An. neivai are reported in this study. Conclusions : Here we report the distribution and genetic diversity of Anopheles species in endemic areas of malaria transmission in Honduras. According to our results, both taxonomic and molecular approaches are useful tools in the identification of anopheline mosquitoes. However, both molecular markers differ in their ability to detect intraspecific genetic diversity. These results provide supporting data for a better understanding of the distribution of malaria vectors in Honduras.

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