scholarly journals Application Efficacy of Vectobac WDG Against Larval Aedes aegypti Using Thermal Fog Technology

2018 ◽  
Vol 34 (1) ◽  
pp. 75-77
Author(s):  
J. A. Knapp ◽  
C. M. Waits ◽  
A. K. C. Briley ◽  
J. E. Cilek ◽  
A. G. Richardson ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Open Field ◽  
Forest Canopy ◽  
Larval Mortality ◽  
Tropical Environment ◽  
Bacillus Thuringiensis Israelensis ◽  
Key West ◽  
Operational Efficacy

ABSTRACT The effectiveness of thermal fog application of Bacillus thuringiensis israelensis (Vectobac® WDG) against larval Aedes aegypti was evaluated in open and forest canopied environments in northeastern Florida, using a handheld IGEBA® TF34 thermal fogger (with water conversion kit). The product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. After application, late-2nd to early-3rd instars of Ae. aegypti were added to each cup. Complete larval mortality occurred 48 h after treatment, 8 m away from the spray line in the open field while 63.4% larval mortality was achieved at this same distance in the forest canopy at 72 h posttreatment. Operational efficacy was then evaluated in an urban tropical environment in Key West, FL. In this study, empty plastic cups were placed in cryptic areas of a commercial lobster and crab trap yard. The 0.1-ha area was treated with Vectobac WDG at the maximum label rate using the same equipment. Aedes aegypti larval mortality from treated cups was >99% at 24 h posttreatment, whereas complete mortality was achieved at 48 h after application.

scholarly journals Ground Applications of Vectobac® WDG with A1 Super-Duty Mist Sprayer® and Micronair® AU5000 Atomizer for Suppression of Aedes aegypti Populations in the Florida Keys

2021 ◽  
Vol 37 (4) ◽  
pp. 271-279
Author(s):  
Heidi L. Murray ◽  
Catherine A. Pruszynski ◽  
Lawrence J. Hribar
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Florida Keys ◽  
Application Rate ◽  
Residential Area ◽  
Distilled Water ◽  
Bacillus Thuringiensis Israelensis ◽  
Key West ◽  
F1 Generation

ABSTRACT Since 2011, the Florida Keys Mosquito Control District (FKMCD) has used the WALS® application strategy with VectoBac® WDG containing Bacillus thuringiensis israelensis via helicopter in Key West for the control of Aedes aegypti larval populations. In 2018, FKMCD conducted a study to determine the effectiveness of using a trailer-mounted A1 Super Duty Mist Sprayer® (A1 Mist Sprayers) with a Micronair® AU5000 (Micron Group) atomizer to apply VectoBac WDG by ground at the rate of 0.5 lb/acre (0.56 kg/ha). Bioassay cups were placed in a residential area encompassing open, moderate, and heavy cover scenarios between 0 and 300 ft (0–91.44 m) perpendicular to the spray line. An application rate of 0.5 lbs/acre (0.56 kg/ha) was used. Bioassay cups were collected after application and returned to the laboratory where 100 ml of distilled water and 10 F1 generation Ae. aegypti larvae were added. Laval mortality was monitored at 2, 4, and 24 h. Three separate runs were completed during the summer of 2018. Average larval mortality at 24 h was >90%. The field trial demonstrated sufficient efficacy to introduce this method of larviciding into operational use.

scholarly journals Efeito residual de apresentações comerciais dos larvicidas temefos e Bacillus thuringiensis israelensis sobre larvas de Aedes aegypti em recipientes com renovação de água

2005 ◽  
Vol 38 (4) ◽  
pp. 316-321 ◽  
Author(s):  
Ricardo José Soares Pontes ◽  
Ana Cláudia Ferreira Regazzi ◽  
José Wellington Oliveira Lima ◽  
Lígia Regina Sansígolo Kerr-Pontes
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Bacillus Thuringiensis Israelensis

Avaliou-se o efeito residual do temefos (apresentações comerciais A, B, C) e Bacillus thuringiensis israelensis (D e E) sobre larvas de Aedes aegypti, em recipientes com renovação de água. Utilizaram-se 44 béqueres de 1.000ml (8 para cada apresentação e 4 controles). Em cada béquer introduziram-se diariamente 25 larvas. Após 24 horas, contavam-se as larvas mortas, esvaziavam-se os béqueres até 200ml, repunha-se o volume original e acrescentavam-se novas larvas. A duração do efeito residual máximo (100% de mortalidade) foi: A-19; B-39; C-40; D-8; E-19 dias. A razão de mortalidade permaneceu equivalente entre todos os larvicidas durante 25 dias; B e C mostraram RM 2,40 vezes maior do que E entre 46-95 dias; B, comparado com A, mostrou RM 1,90-7,51 vezes maior entre 26-95 dias. Conclui-se pela maior eficácia de duas apresentações do temefos, mesmo em uma situação epidemiológica de longa exposição ao produto e com renovação de água dos recipientes.

Cry4Ba and Cyt1Aa proteins from Bacillus thuringiensis israelensis: Interactions and toxicity mechanism against Aedes aegypti

Toxicon ◽  
2015 ◽  
Vol 104 ◽  
pp. 83-90 ◽  
Author(s):  
Jihen Elleuch ◽  
Samir Jaoua ◽  
Frédéric Darriet ◽  
Fabrice Chandre ◽  
Slim Tounsi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Bacillus Thuringiensis Israelensis ◽  
Toxicity Mechanism

scholarly journals Pengaruh Bacillus thuringiensis israelensis Sebagai Larvasida Vektor Demam Berdarah Dengue (DBD) Terhadap Benur Udang Vaname (Litopenaeus vannamei)

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jeany Audina Suryaningkunti ◽  
Endah Setyaningrum ◽  
G. Nugroho Susanto
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Litopenaeus Vannamei ◽  
Bacillus Thuringiensis Israelensis

ABSTRAKPenyakit endemis yang menyebabkan angka kematian tertinggi hampir di seluruh provinsi di Indonesia adalah demam berdarah dengue (DBD). Vektor utama dalam penyebaran penyakit DBD adalah nyamuk Aedes aegypti. Usaha pembibitan udang banyak dikembangkan di Lampung namun usahanya tidak diikuti dengan penyelamatan lingkungan, sehingga muncul wabah DBD dari usaha pembibitan tersebut. Salah satu pengendalian vektor penyakit DBD dengan menggunakan larvasida Bti. Selain harus efektif membunuh larva nyamuk, Bti juga harus aman bagi organisme non target seperti udang vaname (Litopenaeus vannamei).Tujuan dari penelitian ini untuk mengetahui pengaruh Bti sebagai larvasida vektor DBD terhadap mortalitas benur udang vaname (L. vannamei). Penelitian ini dilaksanakan pada bulan November sampai Desember 2018 di Laboratorium Zoologi II, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan yang diberikan yaitu kontrol (tidak diberi Bti), dan penambahan Bti 20 ppm, 40 ppm, 60 ppm, 80 ppm, serta 100 ppm. Parameter yang diamati adalah mortalitas benur udang vaname (L. vannamei), pertumbuhan berupa berat dan panjang, kelulushidupan serta kualitas air selama pemeliharaan. Data pertumbuhan yang diperoleh dianalisis dengan ANOVA, sedangkan data persentase mortalitas, kelulushidupan dan kualitas air yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa Bti dengan berbagai konsentrasi tidak memberikan  pengaruh secara nyata terhadap pertumbuhan (p>0,05), persentase mortalitas dan kelulushidupan benur udang vaname (L. vannamei) sehingga larvasida tersebut dapat digunakan untuk pemberantasan vektor DBD pada hatchery dan tambak udang vaname.Kata kunci : Bacillus thuringiensis israelensis, benur udang vaname (L. vannamei), pertumbuhan, mortalitas, kelulushidupan

scholarly journals Pengaruh Bacillus thuringiensis israelensis Sebagai Larvasida Vektor Demam Berdarah Dengue (DBD) Terhadap Ikan Guppy (Poecilia reticulata)

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Nita Apriyani ◽  
Endah Setyaningrum ◽  
G. Nugroho Susanto
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Poecilia Reticulata ◽  
Bacillus Thuringiensis Israelensis

ABSTRAK Penyakit tular vektor merupakan salah satu masalah kesehatan masyarakat yang utama di Indonesia, diantaranya penyakit Demam Berdarah Dengue (DBD) yang ditularkan oleh Aedes aegypti. Untuk mengurangi efek negatif insektisida, dewasa ini pemberantasan vektor diupayakan dengan penggunaan agen biologi yaitu menggunakan Bacillus thuringiensis israelensis (Bti) yang lebih ramah lingkungan dan tidak menyebabkan resisten vektor. Selain harus efektif membunuh larva nyamuk, Bti juga harus tidak membahayakan biota perairan lainnya yang sehabitat seperti ikan Guppy (Poecilia reticulata). Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh Bti terhadap mortalitas ikan Guppy sebagai organisme non target vektor DBD. Penelitian ini dilakukan di Laboratorium Zoologi, Jurusan Biologi, Fakultas Matematika dan Ilmu pengetahuan Alam, Universitas Lampung. Dilaksanakan pada Desember – Februari 2019. Metode penelitian yang digunakan adalah eksperimental skala laboratoris berupa Rancangan Acak Lengkap (RAL) yaitu konsentrasi Bti sebagai perlakuan dengan empat kali ulangan. Perlakuan yang diuji yaitu kontrol ( tidak diberi Bti), dan pemberian Bti dengan konsentrasi 20 ppm, 40 ppm, 60 ppm, 80 pmm, dan 100 ppm. Hasil penelitian menunjukkan pemberian Bti pada konsentrasi berbeda tidak berpengaruh terhadap kematian ikan Guppy dan tidak berpengaruh secara signifikan terhadap pertumbuhan berat total ikan Guppy.Kata kunci : Demam Berdarah Dengue (DBD), Bacillus thuringiensis israelensis, ikan Guppy.

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