Comparison of Engorged Culex quinquefasciatus Collection and Blood-Feeding Pattern among Four Mosquito Collection Methods in Puerto Barrios, Guatemala, 2007

2010 ◽  
Vol 26 (3) ◽  
pp. 332-336 ◽  
Author(s):  
Rebekah J. Kent ◽  
Ana Silvia Gonzalez Reiche ◽  
Maria Eugenia Morales-Betoulle ◽  
Nicholas Komar
Keyword(s):  
Culex Quinquefasciatus ◽  
Blood Feeding ◽  
Feeding Pattern ◽  
Mosquito Collection ◽  
Collection Methods

scholarly journals Assessment of Mosquito Collection Methods for Dengue Surveillance

2020 ◽  
Author(s):  
Triwibowo Ambar Garjito ◽  
Lulus Susanti ◽  
Mujiyono Mujiyono ◽  
Mega Tyas Prihatin ◽  
Dwi Susilo ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Mosquito Species ◽  
Statistical Significance ◽  
Mixed Data ◽  
Adult Mosquito ◽  
Published Data ◽  
Field Samples ◽  
Mosquito Collection ◽  
Human Landing ◽  
Collection Methods

Abstract BackgroundSeveral methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupal collection, various ovitraps, whole night collection using human landing methods, and larval collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods MethodsA mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used, including morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data (FAMD) was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.ResultsA total of 44,675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2, which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found between either any methods or the incidence.ConclusionsThere was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.

scholarly journals An assessment of mosquito collection techniques for xenomonitoring of anopheline-transmitted Lymphatic Filariasis in Ghana

Parasitology ◽  
2018 ◽  
Vol 145 (13) ◽  
pp. 1783-1791 ◽  
Author(s):  
Millicent Opoku ◽  
Corrado Minetti ◽  
Worlasi D. Kartey-Attipoe ◽  
Sampson Otoo ◽  
Joseph Otchere ◽  
...  
Keyword(s):  
Lymphatic Filariasis ◽  
Close Contact ◽  
Wuchereria Bancrofti ◽  
Northern Region ◽  
Trapping Efficiency ◽  
Mosquito Collection ◽  
Control And Prevention ◽  
Specific Species ◽  
High Degree ◽  
Collection Methods

AbstractMonitoring vectors is relevant to ascertain transmission of lymphatic filariasis (LF). This may require the best sampling method that can capture high numbers of specific species to give indication of transmission. Gravid anophelines are good indicators for assessing transmission due to close contact with humans through blood meals. This study compared the efficiency of an Anopheles gravid trap (AGT) with other mosquito collection methods including the box and the Centres for Disease Control and Prevention gravid, light, exit and BioGent-sentinel traps, indoor resting collection (IRC) and pyrethrum spray catches across two endemic regions of Ghana. The AGT showed high trapping efficiency by collecting the highest mean number of anophelines per night in the Western (4.6) and Northern (7.3) regions compared with the outdoor collection methods. Additionally, IRC was similarly efficient in the Northern region (8.9) where vectors exhibit a high degree of endophily. AGT also showed good trapping potential for collecting Anopheles melas which is usually difficult to catch with existing methods. Screening of mosquitoes for infection showed a 0.80–3.01% Wuchereria bancrofti and 2.15–3.27% Plasmodium spp. in Anopheles gambiae. The AGT has shown to be appropriate for surveying Anopheles populations and can be useful for xenomonitoring for both LF and malaria.

New Mosquito Collection Records for Southern Georgia

2001 ◽  
Vol 36 (2) ◽  
pp. 114-121 ◽  
Author(s):  
John P. Smith ◽  
Thomas G. Floore
Keyword(s):  
Culex Quinquefasciatus ◽  
Species Abundance ◽  
Abundant Species ◽  
Light Traps ◽  
Aedes Vexans ◽  
Detailed Report ◽  
Mosquito Collection ◽  
Abundance And Diversity ◽  
Southern Georgia

Twenty-nine species representing eight genera, numerous new county records and one state record, Mansonia titillans (Walker), were collected in a surveillance project conducted in 20 southern Georgia counties during the summer of 1997. A detailed report of species abundance and diversity is presented. Overall, as sampled by CO2 light traps, Culex quinquefasciatus Say was the most widely distributed species and Aedes vexans (Meigen) was the most abundant species followed closely by Aedes atlanticus Dyer and Knab.

scholarly journals Beat the heat: Culex quinquefasciatus regulates its body temperature during blood-feeding

2020 ◽  
Author(s):  
Joanna M. Reinhold ◽  
Ryan Shaw ◽  
Chloé Lahondère
Keyword(s):  
Heat Stress ◽  
Body Temperature ◽  
Culex Quinquefasciatus ◽  
Evaporative Cooling ◽  
Blood Feeding ◽  
The Body ◽  
List Type ◽  
Physiological Level ◽  
Wide Range ◽  
Warm Blood

AbstractMosquitoes are regarded as one of the most dangerous animals on earth. As they are responsible for the spread of a wide range of both human and animal diseases, research of the underlying mechanisms of their feeding behavior and physiology is critical. Among disease vector mosquitoes, Culex quinquefasciatus, which is a known carrier of West Nile virus and Western Equine Encephalitis, remains relatively understudied. As blood sucking insects, adaptations (either at the molecular or physiological level) while feeding on warm blood is crucial to their survival, as overheating can result in death due to heat stress. Our research aims to study how Cx. quinquefasciatus copes with heat associated with the ingestion of a warm blood-meal and to possibly uncover the adaptations this species uses to avoid thermal stress. Through the use of thermographic imaging, we analyzed the body temperature of Cx. quinquefasciatus while blood feeding. Infrared thermography has allowed us to identify a cooling strategy, evaporative cooling via the production of fluid droplets, and an overall low body temperature in comparison to the blood temperature during feeding. Understanding Cx. quinquefasciatus’ adaptations and various strategies that they employ to reduce their body temperature while blood-feeding constitutes the first step towards the discovery of potential targets of opportunity for their control.HighlightsMosquitoes have evolved to cope with heat stress associated with warm blood ingestionCulex quinquefasciatus displays heterothermy while blood-feedingThe abdominal temperature decreases due to evaporative cooling using urine dropletsOverall, the mosquito body temperature is much cooler than the ingested blood

scholarly journals Assessment of Mosquito Collection Methods for Dengue Surveillance

2020 ◽  
Author(s):  
Triwibowo Ambar Garjito ◽  
Lulus Susanti ◽  
Mujiyono Mujiyono ◽  
Mega Tyas Prihatin ◽  
Dwi Susilo ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Mosquito Species ◽  
Statistical Significance ◽  
Mixed Data ◽  
Adult Mosquito ◽  
Published Data ◽  
Field Samples ◽  
Mosquito Collection ◽  
Human Landing ◽  
Collection Methods

Abstract BackgroundSeveral methods exist to collect and assess the abundance of dengue vector mosquitoes, i.e. morning adult collection using an aspirator, pupal collection, various ovitraps, whole night collection using human landing methods, and larval collection. This diversity of methods might be a source of variability and lack of statistical significance when trying to correlate mosquito density and risk of dengue outbreak. There is also a lack of published data regarding the effectiveness of these methods MethodsA mosquito survey was conducted in 39 locations corresponding to 15 dengue endemic provinces in Indonesia. The larval surveys were performed by collecting at least a single Aedes larva from each container, and then reared up until hatching. Three adult mosquito sampling methods were also used, including morning resting collection, human landing collection, animal baited trap. All field samples were tested for dengue. Factor Analysis of Mixed Data (FAMD) was conducted to analyze the effectiveness of the collection methods against mosquito species and dengue incidence.ResultsA total of 44,675 mosquitoes were collected. The single larva method was the most efficient method. Out of a total of 89 dengue-positive pools, the most frequently encountered virus was DENV2, which made up half of the positive samples, followed by DENV3 and DENV1, respectively. FAMD showed that no correlation could be found between any methods and the presence of dengue virus in mosquitoes. Moreover, no correlation could be found between either any methods or the incidence.ConclusionsThere was no concistency in the efficacy of a given method and the incidence of dengue in the human population. There was no correlation between any of the parameters considered, i.e. method, incidence of dengue, location and presence of dengue virus in mosquitoes. This indicates that entomological factors are not reliable indicators.

Host Feeding Pattern of Culex quinquefasciatus (Diptera: Culicidae) and Its Role in Transmission of West Nile Virus in Harris County, Texas

2007 ◽  
Vol 77 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Goudarz Molaei ◽  
Robert B. Tesh ◽  
Hilda Guzman ◽  
Yvonne Randle ◽  
Susan V. Real ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Culex Quinquefasciatus ◽  
Feeding Pattern ◽  
Host Feeding ◽  
Harris County ◽  
West Nile

scholarly journals Assessment of Synthetic Membranes for Artificial Blood Feeding of Culicidae

Insects ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 15
Author(s):  
Luciana S. Dias ◽  
Jonatas C. Caldeira ◽  
Luiz G. S. R. Bauzer ◽  
José B. P. Lima
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Public Health Problem ◽  
Blood Feeding ◽  
Pathogen Transmission ◽  
Collagen Membrane ◽  
Major Public Health Problem ◽  
Synthetic Membranes ◽  
Artificial Blood ◽  
Potential Pathogen

Potential pathogen transmission through hematophagy in Culicidae is a major public-health problem, and several studies have been performed to better understand this phenomenon. Research on these insects often requires the maintenance of colonies in the laboratory. Due to the hematophagic habits of these organisms, blood must be provided in order to guarantee the reproduction of individuals that constitute the colonies. Some species of mammals and birds are used as a direct blood source in many laboratories. Due to current bioethical parameters, the direct use of animals has been replaced by artificial blood feeding by using synthetic membranes to simulate animal skin. In this study, the efficiency of collagen and latex in the artificial feeding of mosquitoes of the Aedes aegypti and Culex quinquefasciatus species was evaluated and compared with Parafilm®, a standard membrane that is frequently used for this purpose. Important aspects of the feeding and reproduction of these insects were considered. For both species, latex showed the poorest performance. Collagen membrane performed well in most parameters, but was not as efficient as Parafilm® for fecundity in Aedes aegypti, and for the percentage of engorged females in Culex quinquefasciatus. We concluded that, although collagen is more resistant and easier to handle, Parafilm® was the most efficient among the three evaluated membranes for the artificial blood feeding of mosquitoes.

scholarly journals A global assessment of surveillance methods for dominant malaria vectors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bram van de Straat ◽  
Tanya L. Russell ◽  
Kyran M. Staunton ◽  
Marianne E. Sinka ◽  
Thomas R. Burkot
Keyword(s):  
Mosquito Species ◽  
Surveillance Data ◽  
Asia Pacific ◽  
Malaria Vectors ◽  
Asia Pacific Region ◽  
Vector Surveillance ◽  
Host Seeking ◽  
Frequency Of Use ◽  
Mosquito Collection ◽  
Collection Methods

AbstractThe epidemiology of human malaria differs considerably between and within geographic regions due, in part, to variability in mosquito species behaviours. Recently, the WHO emphasised stratifying interventions using local surveillance data to reduce malaria. The usefulness of vector surveillance is entirely dependent on the biases inherent in the sampling methods deployed to monitor mosquito populations. To understand and interpret mosquito surveillance data, the frequency of use of malaria vector collection methods was analysed from a georeferenced vector dataset (> 10,000 data records), extracted from 875 manuscripts across Africa, the Americas and the Asia-Pacific region. Commonly deployed mosquito collection methods tend to target anticipated vector behaviours in a region to maximise sample size (and by default, ignoring other behaviours). Mosquito collection methods targeting both host-seeking and resting behaviours were seldomly deployed concurrently at the same site. A balanced sampling design using multiple methods would improve the understanding of the range of vector behaviours, leading to improved surveillance and more effective vector control.

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