scholarly journals Effect of upstream DNA architecture on transcription of a human LINE-1 retrotransposon sequence.

2001 ◽  
Vol 13 (1/2) ◽  
pp. 1-6
Author(s):  
Masaru MIYANO ◽  
Takahiro OKABE ◽  
Takahiro KUSAKABE
Keyword(s):  
Retrotransposon Sequence ◽  
Human Line

Quantification of expression of linked cloned genes in a simian virus 40-transformed xeroderma pigmentosum cell line

1985 ◽  
Vol 5 (7) ◽  
pp. 1685-1693
Author(s):  
M Protić-Sabljić ◽  
D Whyte ◽  
J Fagan ◽  
B H Howard ◽  
C M Gorman ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Lines ◽  
Xeroderma Pigmentosum ◽  
Phenotypic Expression ◽  
Simian Virus 40 ◽  
Fibroblast Cell ◽  
Simian Virus ◽  
Selective Medium ◽  
Normal Human ◽  
Human Line

We wished to determine whether simian virus 40 (SV40)-transformed xeroderma pigmentosum cells, despite their defective DNA repair, were suitable for DNA-mediated gene transfer experiments with linked genes. Expression of a nonselectable gene (cat, coding for chloramphenicol acetyltransferase [CAT]) linked to a selectable gene (gpt, coding for xanthine-guanine phosphoribosyltransferase [XPRT]) in the plasmid pSV2catSVgpt was quantified after transfection of SV40-transformed xeroderma pigmentosum [XP20s(SV40)] and normal human [GM0637(SV40)] fibroblast cell lines. A novel autoradiographic assay with [3H]xanthine incorporation showed 0.5 to 0.7% phenotypic expression of XPRT in both cell lines. Without selection, transient CAT activity was 20 times greater in the GM0637(SV40) than in the XP20s(SV40) cells, and transient XPRT activity was 5 times greater. Both of these transient activities were increased and equalized in both cell lines by transfection with pRSVcat or pRSVgpt. Genotypic transformation to gpt+ occurred at a frequency of 2 X 10(-4) to 4 X 10(-4) in both cell lines with pSV2catSVgpt. After 2 to 3 months in selective medium, stable expression of the (nonselected) cat gene was found in 11 (92%) of 12 gpt-containing clones derived from GM0637(SV40) cells and in 13 (81%) of 16 gpt-containing clones from XP20s(SV40) cells. However, the levels of CAT activity did not correlate with those of XPRT activity, and both of these activities varied more than 100-fold among different clones. Copies (1 to 4) of the gpt gene were integrated in four clones of the GM0637(SV40) cells having an XPRT activity of 1 to 5 nmol/min per mg, but 5 to 80 copies were integrated in four XP20s(SV40) clones with an XPRT activity of 0.8 to 1.8 nmol/min per mg. This study shows that XP20s(SV40) is as suitable for gene transfer experiments as the normal human line GM0637(SV40).

scholarly journals The Female Line in the Bible. Ratzinger’s Deepening of the Church’s Understanding of Tradition and Mary

Religions ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 310
Author(s):  
Mary Frances McKenna
Keyword(s):  
Holy Spirit ◽  
Full Potential ◽  
Human Being ◽  
Salvation History ◽  
Adam And Eve ◽  
The Bible ◽  
Female Line ◽  
Dei Verbum ◽  
Male Line ◽  
Human Line

This paper explores the female line in the Bible that Joseph Ratzinger identifies as running in parallel to, and being indispensable for, the male line in the Bible. This female line expands the understanding of Salvation History as described by Dei Verbum so that it runs not just from Adam through to Jesus, but also from Adam and Eve to Mary and Jesus, the final Adam. Ratzinger’s female line demonstrates that women are at the heart of God’s plan for humanity. I illustrate that this line is evident when Ratzinger’s method of biblical interpretation is applied to the women of Scripture. Its full potential comes into view through Ratzinger’s development of the Christian notion of person: Person as revealed by Jesus Christ is relatedness without reserve with God and is fully applicable to the human being through Christ. I argue that together, the male and female lines in the Bible form the human line in the Bible, in which the male line represents “the humanity”, every human being, while the female line represents the communal aspect of humanity. Moreover, I contend that Christianity’s notion of mother in relation to God (as Father, Son and Holy Spirit) should be understood through Mary’s response at the Annunciation. Mother in relation to God is to be understood through the Incarnation when Mary, as person, lived her life wholly in relation with and for God.

scholarly journals Lung genotoxicity of benzo(a)pyrene in vivo involves reactivation of LINE-1 retrotransposon and early reprogramming of oncogenic regulatory networks

2019 ◽  
Vol 317 (6) ◽  
pp. L816-L822
Author(s):  
A. A. I. Hassanin ◽  
M. Tavera-Garcia ◽  
B. Moorthy ◽  
G. D. Zhou ◽  
K. S. Ramos
Keyword(s):  
Regulatory Network ◽  
Regulatory Networks ◽  
Intratracheal Instillation ◽  
Cre Recombinase ◽  
Oncogenic Signaling ◽  
Nucleosome Remodeling ◽  
Expression Of Genes ◽  
Dna Deletions ◽  
Human Line

Several lines of evidence have implicated long interspersed nuclear element-1 (LINE-1) retroelement in the onset and progression of lung cancer. Retrotransposition-dependent mechanisms leading to DNA mobilization give rise to insertion mutations and DNA deletions, whereas retrotransposition-independent mechanisms disrupt epithelial programming and differentiation. Previous work by our group established that tobacco carcinogens such as benzo(a)pyrene (BaP) reactivate LINE-1 in bronchial epithelial cells through displacement of nucleosome remodeling and deacetylase (NuRD) corepressor complexes and interference with retinoblastoma-regulated epigenetic signaling. Whether LINE-1 in coordination with other genes within its regulatory network contributes to the in vivo genotoxic response to BaP remains largely unknown. Evidence is presented here that intratracheal instillation of ORFeusLSL mice with BaP alone or in combination with adenovirus (adeno)-CRE recombinase is genotoxic to the lung and associated with activation of the human LINE-1 transgene present in these mice. LINE-1 reactivation modulated the expression of genes involved in oncogenic signaling, and these responses were most pronounced in female mice compared with males and synergized by adeno-CRE recombinase. This is the first report linking LINE-1 and genes within its oncogenic regulatory network with early sexually dimorphic responses of the lung in vivo.

scholarly journals Nucleoside Analogue Reverse Transcriptase Inhibitors Differentially Inhibit Human LINE-1 Retrotransposition

PLoS ONE ◽  
2008 ◽  
Vol 3 (2) ◽  
pp. e1547 ◽  
Author(s):  
R. Brad Jones ◽  
Keith E. Garrison ◽  
Jessica C. Wong ◽  
Erick H. Duan ◽  
Douglas F. Nixon ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Nucleoside Analogue ◽  
Reverse Transcriptase Inhibitors ◽  
Human Line

Human LINE retrotransposons generate processed pseudogenes

10.1038/74184 ◽  
2000 ◽  
Vol 24 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Cécile Esnault ◽  
Joël Maestre ◽  
Thierry Heidmann
Keyword(s):  
Processed Pseudogenes ◽  
Human Line

scholarly journals Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein

Mobile DNA ◽  
2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Mark Sokolowski ◽  
Cecily B DeFreece ◽  
Geraldine Servant ◽  
Kristine J Kines ◽  
Dawn L deHaro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Orf2 Protein ◽  
Endonuclease Domain ◽  
Human Line
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