scholarly journals Anaerobic Bioremediation of Tetrachloroethylene-contaminated Soil by Using an Anaerobic Enrichment Culture.

2000 ◽  
Vol 23 (4) ◽  
pp. 232-237
Author(s):  
Toshiya KOMATSU ◽  
Tsuyoshi DOHI ◽  
Kiyoshi MOMONOI
Keyword(s):  
Contaminated Soil ◽  
Enrichment Culture ◽  
Anaerobic Bioremediation

Full-scale anaerobic bioremediation of trinitrotoluene (TNT) contaminated soil

1995 ◽  
Vol 51-52 (1) ◽  
pp. 625-633 ◽  
Author(s):  
S. B. Funk ◽  
D. L. Crawford ◽  
R. L. Crawford ◽  
G. Mead ◽  
W. Davis-Hoover
Keyword(s):  
Contaminated Soil ◽  
Full Scale ◽  
Anaerobic Bioremediation

scholarly journals Degradation of pendimethalin by the yeast YC2 and determination of its two main metabolites

RSC Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 491-497 ◽  
Author(s):  
Yajie Han ◽  
Zonggui Tang ◽  
Huifang Bao ◽  
Dongmei Wu ◽  
Xiaolin Deng ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Yeast Strain ◽  
Enrichment Culture ◽  
Soil P

In this study, we isolated a yeast strain, YC2, by enrichment culture from pendimethalin-contaminated soil.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil

1994 ◽  
Vol 40 (6) ◽  
pp. 432-438 ◽  
Author(s):  
William T. Stringfellow ◽  
Michael D. Aitken
Keyword(s):  
Contaminated Soil ◽  
Genetic Relatedness ◽  
Enrichment Culture ◽  
Acid Methyl Ester ◽  
Pseudomonas Stutzeri ◽  
Maximum Activity ◽  
Resting Cells ◽  
Phenanthrene Degradation ◽  
Naphthoic Acid ◽  
P 16

Two species of bacteria, identified as Pseudomonas stutzeri (P-16) and Pseudomonas saccharophila (P-15) by fatty acid methyl ester analysis, were found in a phenanthrene enrichment culture of a creosote-contaminated soil. The organisms are shown to be physiologically dissimilar, and their genetic relatedness is discussed. Phenanthrene degradation by both organisms followed Michaelis–Menten kinetics, allowing for the determination of half-saturation (Ks) and maximum activity coefficients, using nonlinear regression. Both organisms utilized kinetically similar enzymes for phenanthrene uptake and oxidation, as evidenced by similar Ks coefficients of approximately 0.2 mg/L and temperature optima of 40 °C, but levels of expression differed with different media. Each organism degraded phenanthrene via salicylic acid, but patterns of intermediate metabolism were shown to differ. P-15 excreted 1-hydroxy-2-naphthoic acid during growth on phenanthrene and demonstrated Michaelis–Menten kinetics for the oxidation of 1-hydroxy-2-naphthoic acid by resting cells. P-16 excreted only trace amounts of 1-hydroxy-2-naphthoic acid and demonstrated linear kinetics in response to 1-hydroxy-2-naphthoic acid concentration. P-15 was found to form thick biofilms on phenanthrene crystals and was characterized by a hydrophobic cell surface, whereas P-16 grew mostly in suspension and was hydrophilic. Neither organism produced significant amounts of biosurfactants when grown on phenanthrene. The implications of these findings for the design of systems to remediate contaminated soil are discussed.Key words: phenanthrene, 1-hydroxy-2-naphthoic acid, biodegradation, kinetics, polycyclic aromatic hydrocarbons.

scholarly journals Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil

2014 ◽  
Vol 3 (2) ◽  
pp. 63-75 ◽  
Author(s):  
HY Ismail ◽  
UJJ Ijah ◽  
ML Riskuwa ◽  
II Allamin
Keyword(s):  
Molecular Weight ◽  
Contaminated Soil ◽  
Rhizosphere Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Lablab Purpureus ◽  
Degrading Bacteria ◽  
Spent Engine Oil

Biodegradation of spent engine oil (SEO) by bacteria isolated from the rhizosphere of Cajan cajan and Lablab purpureus was investigated. It was with a view to determining most efficient bacterial species that could degrade SEO in phytoremediation studies. Hydrocarbon degrading bacteria were isolated and identified by enrichment culture technique using oil agar supplemented with 0.1% v/v SEO. Total heterotrophic and oil utilizing bacterial count showed the occurrence of large number of bacteria predominantly in the rhizosphere soil, ranging between 54×108 - 144×108 CFU/g and 4×108- 96×108 CFU/g respectively. Percentage of oil utilizing bacteria ranged between 0% (uncontaminated non rhizosphere soil) to 76% (contaminated rhizosphere). Turbidimetrically, five bacterial species namely Pseudomonas putrefacience CR33, Klebsiella pneumonia CR23, Pseudomonas alcaligenes LR14, Klebsiella aerogenes CR21, and Bacillus coagulans CR31 were shown to grow maximally and degraded the oil at the rate of 68%, 62%, 59%, 58%and 45% respectively. Chromatographic analysis using GC-MS showed the presence of lower molecular weight hydrocarbons in the residual oil (indicating degradation) after 21 days, whereas the undegraded oil (control) had higher molecular weight hydrocarbons after the same period. The species isolated were shown to have high ability of SEO biodegradation and therefore could be important tools in ameliorating SEO contaminated soil. DOI: http://dx.doi.org/10.3126/ije.v3i2.10515 International Journal of the Environment Vol.3(2) 2014: 63-75

scholarly journals An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

2019 ◽  
Vol 68 (2) ◽  
pp. 193-201 ◽  
Author(s):  
FATIMA MUCCEE ◽  
AMINA EJAZ
Keyword(s):  
Contaminated Soil ◽  
Phylogenetic Trees ◽  
High Efficiency ◽  
Thermophilic Bacteria ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Soil Samples ◽  
Biochemical Characteristics ◽  
Antibiotic Resistance Profile ◽  
Fermentation Test

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

Biodegradation of heptadecane in hydrocarbon polluted dune sands using a newly-isolated thermophilic bacterium, Brevibacillus borstelensis TMU30: statistical evaluation and process optimization

RSC Advances ◽  
2015 ◽  
Vol 5 (42) ◽  
pp. 33414-33422 ◽  
Author(s):  
Bahram Khazra ◽  
Seyyed Mohammad Mousavi ◽  
Sadaf Mehrabi ◽  
Maryam Hashemi ◽  
Seyed Abbas Shojaosadati
Keyword(s):  
Process Optimization ◽  
Contaminated Soil ◽  
Statistical Evaluation ◽  
Petroleum Refinery ◽  
Enrichment Culture ◽  
Thermophilic Bacterium ◽  
Soil Samples ◽  
Dune Sands ◽  
Degrading Bacterium

An enrichment culture was established to isolate a thermophilic hydrocarbon-degrading bacterium from contaminated soil samples from the Tehran Petroleum Refinery.

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