scholarly journals Pro- and Antioxidant Activity in the Blood of Adolescents of Different Ethnic Groups

2019 ◽  
Vol 3 (6) ◽  
pp. 23-28
Author(s):  
N. A. Kurashova ◽  
E. A. Kudeyarova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
The Body ◽  
Endocrine Disorders ◽  
Antioxidant Protection ◽  
Secondary Products ◽  
Adaptive Capabilities ◽  
Conjugated Trienes

Background. Processes of antioxidant protection in different ethnicities, living in the same geographical conditions, are of undoubted scientific interest. The ratio of activity of oxidative processes and antioxidant components not only reflects, but also largely determines the metabolism and adaptive capabilities of the organism. The key issue in understanding the regulatory and adaptive role of lipid peroxidation reactions in the body is the idea of the existence of a certain balance with the antioxidant defense system.Aims: to study the features of the system of lipid peroxidation and antioxidant protection in adolescents of different ethnicities, living in Eastern Siberia.Materials and methods. Parameters of lipid peroxidation and antioxidant protection were determined in the blood serum by spectrophotometric methods.Results. We found that adolescent Buryat boys had statistically significant decrease in primary and secondary products of lipid peroxidation: ketodienes and conjugated trienes, malondialdehyde, and components of antioxidant protection: retinol, α-tocopherol, the level of total antioxidant activity of blood and reduced glutathione, in comparison with Caucasian adolescents. In Caucasian adolescents with endocrine disorders, there was a statistically significant increase in the level of ketodienes and conjugated trienes, as well as the activity of superoxide dismutase against the background of a decrease of reduced glutathione, tocopherol, and retinol. We revealed a significant increase in the concentration of ketodienes and conjugated trienes and a decrease of the oxidized glutathione in adolescents with endocrine disorders in comparison with healthy adolescents of Buryat ethnicity.Conclusions. Studying of the processes of lipid peroxidation and antioxidant system of the organism can be used as an additional criterion for complex examination of practically healthy adolescents, which significantly enhance the representation about the adaptation possibilities of the organism to external conditions and can become a basis for effective examination of reproductive health in the future.

scholarly journals INFLUENCE OF «METISEVIT» ON THE ACTIVITY OF ENZYME AND NONENZYME LINK OF ANTIOXIDANT PROTECTION UNDER THE BULL’S BODY CADMIUM LOADING

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 52-59
Author(s):  
B.V. Gutyj ◽  
Y. Lavryshyn ◽  
V. Binkevych ◽  
O. Binkevych ◽  
О. Paladischuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Cadmium Stress ◽  
Antioxidant Defense System ◽  
The Body ◽  
Antioxidant Protection ◽  
Young Cattle

The article contains the research results of the effect of cadmium chloride on the indexes of enzyme and nonenzyme systems of  antioxidant defense system in young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E. It is established that feeding calves at a dose of toxicant 0.04 mg / kg activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E in the blood of experimental animals decreased throughout the experiment. The lowest indicators of antioxidant in the blood of young cattle is set on the twenty -fourth day of the experiment, which is associated with increased activation of lipid peroxidation and the balance between antioxidant system and lipid peroxidation intensity. Given the cadmium load of young cattle it is used a new integrated drug with antioxidant action «Metisevit», which includes metifen, sodium selenite and vitamin E wich is founded as stimulating effects on the activity of antioxidant protection. In particular,it is established probable increase in activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, vitamin A and vitamin E in the blood of young cattle, which has performed cadmium stress. These changes occur through comprehensive action components of the drug «Metisevit» that leads to the normalization of metabolic processes and free radical in the body of the bull. The results of the research indicate antioxidant drug «Metisevit» in the application of its young cattle and the validity of his administration to improve the body's antioxidant status of chronic cadmium toxicosis.

scholarly journals Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

2019 ◽  
Vol 4 (1) ◽  
pp. 14-18
Author(s):  
Nadezhda A. Kurashova ◽  
Ekaterina A. Kudeyarova ◽  
Ekaterina O. Kuznetsova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Antioxidant Protection ◽  
Defense System ◽  
Total Antioxidant ◽  
Peroxidation Processes

Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

scholarly journals Influence of feed additive “Butaselmevit-Plus” on antioxidant status of rats in conditions of oxidative stress

2019 ◽  
Vol 21 (90) ◽  
pp. 76-81 ◽  
Author(s):  
T. V. Martyshuk ◽  
B. V. Gutyj
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Antioxidant Properties ◽  
The Body ◽  
Feed Additive ◽  
Antioxidant Protection ◽  
Feed Supplement ◽  
Experimental Group

The purpose of the work was to investigate the effect of the feed supplement “Butaselmevit Plus” on the antioxidant status of the organism in rats under conditions of oxidative stress. The research was carried out on white, sexually-mature, young male rats of the Wistar line, with a body weight of 180–200 g, which was kept on a standard diet of the Institute vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Supplements. The animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; Group 2 (R1) – rats, affected with tetrachloromethane; Group 3 (R2) – rats, affected with tetrachloromethane and used as a feed additive “Butaselmevit-Plus”. The feed supplement “Butaselmevit-Plus” includes the fruits of thistle spotted, methionine, sodium selenite and vitamins A, E, D3. Significant violation of oxidative-antioxidant balance in animals under oxidative stress conditions, which is characterized, primarily, by activation of processes of radical lipid oxidation with excessive accumulation of both intermediate and final products of lipid peroxidation oxidation and inhibition of antioxidant defense system activity, is established. The development of oxidative stress leads to the inhibition of the activity of the glutathione system of antioxidant protection of the body of rats. This is evidenced by the low activity of glutathione peroxidase and the low level of reduced glutathione in the blood of experimental rats. The feed supplement “Butaselmeib-Plus” contributed to the activation of the system of antioxidant protection of the body of rats for tetrachloromethane poisoning, as evidenced by the increased activity of glutathione peroxidase and the level of reduced glutathione. In addition, in the blood of experimental rats, inhibition of lipid peroxidation and formation of free radicals was observed. It was determined that the level of lipids hydroperoxides on the 20th day of the experiment in blood of rats of experimental group R2 decreased by 35.7%, and the level of TBK-active products – by 21.6% relative to the indices of the first experimental group of rats. Thus, the feed supplement “Butaselmevit-Plus” when fed to rats for the development of oxidative stress inhibited lipid peroxidation, as indicated by the low level of lipids hydroperoxides and TBK-active products in their blood. This may be due to the fact that the feed additive includes two strong antioxidants, such as vitamin E and selenium, which in turn enhance the action of each other. It should also be noted that the antioxidant properties of thistle blisters, which, according to the literature, also possess antioxidant properties. It consists of vitamins B, A, E, K, precursors of vitamin D, carotenoids, macroelements – potassium, calcium, magnesium, ferrum and microelements – Cuprum, Zinc, Manganese, Iodine. The combined action of these biologically important elements exhibits high hepatoprotective and antioxidant activity.

scholarly journals ETHNIC FEATURES OF LIPID PROFILE AND LIPID PEROXIDATION IN FREESTYLE WRESTLERS

2019 ◽  
Vol 19 (2) ◽  
pp. 37-44
Author(s):  
N Kurashova ◽  
A Yureva ◽  
M Dolgikh ◽  
I Gutnik ◽  
L Kolesnikovа
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Profile ◽  
Ethnic Group ◽  
Ethnic Groups ◽  
Reduced Glutathione ◽  
The Body ◽  
Antioxidant Protection ◽  
Physiological Basis ◽  
Lipid Exchange ◽  
Metabolic Intensity

Aim. The article deals with the assessment of the lipid profile, lipid peroxidation, and antioxidant protection in freestyle wrestlers belonging to different ethnic groups. Materials and methods. The study involved freestyle male wrestlers aged 18–22 with the rank of the Candidate for Master of Sport and Master of Sport regularly practicing freestyle wrestling. We studied a serum consisting of blood plasma and erythrocyte hemolysate. Lipid exchange was studied with the help of the BTS-350 analyzer (Spain) and BioSystems (Spain) reagents. To establish aminotransferase in blood serum, we used Vital (Russia) reagents. The components of lipid peroxidation and antioxidant protection were established with the help of spectrophotometric and fluorometric methods. Results. Freestyle athletes of the Russian ethnic group are characterized by a significantly lower TG concentrations compared to Buryat athletes. In the system of lipid peroxidation and antioxidant protection, in athletes of the Russian ethnic group, we revealed a significant AOA, reduced glutathione and retinol compared to Buryat athletes. Conclusion. The data obtained prove a different degree of metabolic activity in athletes from various ethnic groups. The balance between lipid oxidation and antioxidant protection mostly determines metabolic intensity as well as adaptation capacities of the body. The study of metabolic changes in highly-skilled athletes from different ethnic groups can contribute to the deepening of knowledge about the physiological basis and pathogenetic effects of stress on the human body. This will be one of the factors for optimizing training and providing the search for the ways of increasing physical activity as a mean of preserving the nation and sports development.

scholarly journals Hibiscus sabdariffaAffects Ammonium Chloride-Induced Hyperammonemic Rats

2007 ◽  
Vol 4 (3) ◽  
pp. 321-325 ◽  
Author(s):  
M. Mohamed Essa ◽  
P. Subramanian
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ammonium Chloride ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Alcoholic Extract ◽  
Hibiscus Sabdariffa ◽  
Protein Nitrogen ◽  
Antioxidant Effects ◽  
Brain Tissues

Hibiscus sabdariffa(HS) is an edible medicinal plant, indigenous to India, China and Thailand and is used in Ayurveda and traditional medicine. Alcoholic extract of HS leaves (HSEt) was studied for its anti-hyperammonemic and antioxidant effects in brain tissues of ammonium chloride-induced hyperammonemic rats. Oral administration of HSEt (250 mg kg−1body weight) significantly normalizes the levels of ammonia, urea, uric acid, creatinine and non-protein nitrogen in the blood. HSEt significantly reduced brain levels of lipid peroxidation products such as thiobarbituric acid and reactive substances (TBARS) and hydroperoxides (HP). However, the administered extract significantly increased the levels of antioxidants such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) in brain tissues of hyperammonemic rats. This investigation demonstrates significant anti-hyperammonemic and antioxidant activity of HS.

Indicators of chemiluminescent and bioluminescent tests of biological liquids in the assessment of physical health

2020 ◽  
Vol 65 (9) ◽  
pp. 541-546
Author(s):  
Svetlana Nikolaevna Derevtsova ◽  
A. A. Romanenko ◽  
O. A. Kolenchukova ◽  
L. V. Stepanova ◽  
V. G. Nikolaev ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Young Male ◽  
Antioxidant Defense System ◽  
The Body ◽  
Antioxidant Protection ◽  
Body Type ◽  
Sexual Characteristics ◽  
Body Sizes ◽  
Sex Inversion ◽  
Biological Liquids

The study includes anthropometry of 172 young male, obtained data on the length and body mass, measured the transverse diameters of the shoulders and pelvis, various body types was identified by the J.M. Tanner sexual dimorphism index (andromorphic, mesomorphic, gynecomorphic). The chemiluminescent and bioluminescent study of saliva and blood was conducted in the examined young male. We studied the indicators of the antioxidant defense system under the influence of stress. The antioxidant status of saliva was determined using the H2O2-luminol-dependent chemiluminescence method. Data on the activity of NAD (P) -dependent dehydrogenases in blood lymphocytes was obtained from a bioluminescent method of research. Young male of andromorphic body type had large overall and transverse body sizes. Indicators of antioxidant protection of saliva and blood in men of adolescence, the body type of the sexual dimorphism index J.M. Tanner was different. The persons of the andromorphic body type differed in terms of chemiluminescence in comparison with the young male of gynecomorphic body type. The results of bioluminescent blood tests suggest a violation of the catabolic and anabolic processes of carbohydrate and fat metabolism in young men of mesomorphic and gynecomorphic body types. Indicators of the system of antioxidant protection of saliva and blood reflect the sexual characteristics of the body of young male and can be used as additional criteria for diagnosing sex inversion and assessing the risk of developing socially attributed diseases.

scholarly journals FEATURES OF LIPID PEROXIDATION-ANTIOXIDANT PROTECTION IN ADOLESCENT GIRLS IN DIFFERENT GROUPS OF HEALTH

2015 ◽  
Vol 69 (3-4) ◽  
pp. 50-54
Author(s):  
L. I. Kolesnikova ◽  
B. Ya. Vlasov ◽  
O. V. Kravtsova ◽  
M. I. Dolgikh ◽  
L. V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Adolescent Girls ◽  
Antioxidant Defense System ◽  
Health State ◽  
Oxidized Glutathione ◽  
Antioxidant Protection ◽  
High Concentration ◽  
The Third ◽  
Unsaturated Lipids ◽  
The Village

Among the metabolic processes involved in the formation of physical and sexual development of adolescents recently the system of lipid peroxidation and antioxidant protection (LPO-AOP) supporting the redox state of organism and its adaptive response under the influence of different factors is highlighted. Background: The aim of this work is an analysis LPO-AOP-parameters in adolescent girls of different groups of health depending on where they live. Patients and methods: We examined 143 girls aged 15–17 years who had the second and the third groups of health and lived in the village and the city. For assessment of LPO-AOP parameters spectrophotometric, spectrofluorometric and statistical methods were used. Results: The combination of high concentration of unsaturated lipids with primary accumulation of lipid peroxidation products and the stable level of the end products of lipid peroxidation were found in girls rural both groups of health. State of the antioxidant defense system in girls of the village was characterized by a high content of its components, except for α-tocopherol. It concentration was reduced in girls of the third group of health. The levels of lipid peroxidation secondary intermediats were elevated and oxidized glutathione level was reduced in city girls of the third groups of health. Conclusion: The usage of oxidized glutathione for the prevention of exacerbations of chronic diseases in city girls of the third group of health is recommended.

scholarly journals The ratio of erythrocyte populations in the blood of puppies during postnatal oxidative stress

2019 ◽  
pp. 81-87
Author(s):  
M. Anfiorova ◽  
M. Broshkov ◽  
O. Danchuk
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Key Words ◽  
Schiff Bases ◽  
The Body ◽  
Peroxide Oxidation ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
End Products ◽  
Conjugated Trienes

The article presents new scientifi c data on the ratio of erythrocyte populations in puppies' blood during postnatal oxidative stress. It was established that the number of erythrocytes in the blood of one-day-old puppies was 5.64 ± 0.12 T / l, and half of these cells belonged to the population of "mature", 40% - "young" and 10% - "old". In day-old puppies, the content of lipid peroxidation products in erythrocyte hemolysates is quite high, which indicates the development of postnatal oxidative stress. Thus, the content of diene conjugates, ketodienes and Schiff bases is respectively 1,406 ± 0,023 Conv. units, 0.676 ± 0.004 Conv. units and 0.135 ± 0.003 Conv. units. From one to fi ve days age of puppies the number of erythrocytes in the blooddecrease by 1.5 times (p<0.001), mainly due to the decrease in the number of "mature" cells (by 1.6 times; p<0.001). The intensity of peroxide oxidation in the body of puppies even a month after birth is relatively high, as indicated by the content of Schiff bases in the erythrocytes of the blood of these animals - 0.185 ± 0.002 Conv.units. The inverse correlations of the content of Schiff bases in erythrocyte hemolysate with their number in the bloodstream were found to be r = -0.83 (p<0.001). It should also be noted the direct correlation of the content of diene conjugates in the blood of puppies with the number of erythrocytes in their blood (r = 0,58 p <0,05), but the content of ketodienes and conjugated trienes is inversely related to the content of "young" erythroid cells in the puppies' blood (r = -0.57 p <0.05). Consequently, post-natal adaptation of puppies results in the replacement of fetal erythrocytes by postnatal cells, and this process is associated with the intensifi cation of peroxide oxidation and accumulation of the end products of peroxidationin the cells. This process isaccompanied by an acceleration of the process of "aging" of young forms of erythrocytes, as a result of which prerequisites for the development of anemiaare created. Key words: erythrocytes, oxidative stress, puppies, Schiff bases, ketodienes, diene conjugates.

Effect of Plasmodium and Salmonella co-infection in a murine model

Open Medicine ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. 340-347 ◽  
Author(s):  
Geeta Shukla ◽  
Divya Singh ◽  
Lalita Sharma ◽  
Ashwani Koul ◽  
Praveen Rishi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Bacterial Load ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Control Groups ◽  
Biochemical Alterations ◽  
Selection Of

AbstractThe present study was designed to evaluate the effect of Plasmodium and Salmonella co-infection in LACA mice. The parasitaemic level, bacterial load, histological alterations and levels of oxidants/antioxidant activity were measured. Co-infected mice had a high parasitaemic level, increased bacterial load, and died earlier than Plasmodium-infected mice. Histologically, co-infected mice had more architectural damage in the liver, spleen, kidney, and brain than the control groups. The level of lipid peroxidation was significantly increased and the activities of antioxidative enzymes (superoxide dismutase and catalase) were decreased in all organs of co-infected mice compared to the control groups, indicating depression of the antioxidant defense system. The present study demonstrates more severe histological and biochemical alterations in co-infected mice, highlighting the importance of early diagnosis for selection of appropriate treatments and reducing the likelihood of further complications.

scholarly journals Specific features of biological oxidation processes in helminthiases of horses

2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The Body ◽  
Luminous Intensity ◽  
Radical Oxidation ◽  
Pathological Conditions ◽  
Oxidized Products

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.

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