scholarly journals THE DYNAMICS OF ORTHOHANTAVIRUS HANTAAN STRAINS REPLICATION ON THE MODEL OF MOUSE PERITONEAL MACROPHAGES

2018 ◽  
Vol 3 (4) ◽  
pp. 47-52
Author(s):  
A. B. Pott ◽  
O. V. Iunikhina ◽  
I. N. Lyapun ◽  
G. G. Kompanets
Keyword(s):  
Cell Culture ◽  
Fluorescent Antibody ◽  
Hemorrhagic Fever ◽  
Peritoneal Macrophages ◽  
Hantaan Virus ◽  
Field Of Vision ◽  
Significant Difference ◽  
Apodemus Mice ◽  
Mouse Peritoneal Macrophages

Orthohantaviruses (Orthohantavirus genus, Hantaviridae family) are the causative agents of a widespread natural focal infection in the  Russian Federation, hemorrhagic fever with renal syndrome (HFRS).  An important role in the persistence of orthohantavirus in reservoir  hosts among other immunological responses, as well as in the  spread of the virus in the infected organism, is played by infected  macrophages, which, along with the vascular endothelium, are the main targets for orthohantaviruses.The aimof our study was to investigate the characteristics of replication of orthohantavirus Hantaan strains isolated from  Apodemus mice and detect the influence of different values of  multiplicity of infection (MOI) on replication dynamics of orthohantaviruses on cell culture.Materials and methods. We used 4 strains of Hantaan virus, isolated from A. agrarius (n = 2) and A. peninsulae (n = 2), captured in the different areas of Primorsky Krai of Russia. The  modeling of infection was performed on the primary cell culture of  mouse peritoneal macrophages with different MOI (from 10 to 0.1).  The assessing of infection was conducted via indirect fluorescent  antibody assay, and results were expressed as rate of antigen- positive cells per all cells in the field of vision. Results.Common dynamics of orthohantavirus infection on this in vitro model was characterized by periodically increased rates of  infected cells after 2, 4, 6 и 8 hours post infection (p.i.). Replication  of A. agrarius-borne strains was more intensive compare with  viruses, isolated from A. peninsulae, in the time point 4 hour p.i. on  the background the same MOI the statistically significant difference  of rate of antigen-positive cell 24.9 ± 2.38 % vs 15.2 ± 1.87 % (t =  3.20; p = 0.001414) was observed. Additionally, the decrease of MOI was followed by determined decrease of replication effectivity.Conclusion. The results of our study showed the significant phenotyping heterogeneity of orthohantavirus Hantaan strains,  isolated from Apodemus mice, resulting in different rates of  replication in the culture of mouse peritoneal macrophages.

scholarly journals A Critical Role of Activin A in Maturation of Mouse Peritoneal Macrophages in vitro and in vivo

2009 ◽  
Vol 6 (5) ◽  
pp. 387-392 ◽  
Author(s):  
Yinan Wang ◽  
Xueling Cui ◽  
Guixiang Tai ◽  
Jingyan Ge ◽  
Nan Li ◽  
...  
Keyword(s):  
Critical Role ◽  
Peritoneal Macrophages ◽  
Activin A ◽  
Mouse Peritoneal Macrophages

scholarly journals Immunomodulatory Action of Triterpene Glycosides Isolated from the Sea Cucumber Actinocucumis typica. Structure-Activity Relationships

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Evgeny A. Pislyagin ◽  
Dmitry L. Aminin ◽  
Alexandra S. Silchenko ◽  
Sergey A. Avilov ◽  
Pelageya V. Andryjashchenko ◽  
...  
Keyword(s):  
Peritoneal Macrophages ◽  
Triterpene Glycosides ◽  
Cytotoxic Activities ◽  
Ros Formation ◽  
Low Toxicity ◽  
Low Cytotoxicity ◽  
Immunomodulatory Action ◽  
Mouse Peritoneal Macrophages ◽  
Stimulation Of

Stimulation of lysosomal activity and ROS formation in mouse peritoneal macrophages by five triterpene glycosides, typicosides A1 (1), A2 (2), B1 (3), C1 (4) and C2 (5) has been studied and compared with their cytotoxic activities. Glycosides 1–3 possess moderate activities, but the most cytotoxic glycoside 5 is not active. Typicoside C1 (4), with low toxicity, was proved to be the most active concerning stimulation of ROS formation. This is the first example of a triterpene glycoside from sea cucumbers with low cytotoxicity, but which demonstrates a strong immunostimulatory effect on mouse peritoneal macrophages in vitro.

scholarly journals Dynamics of leukotriene C production by macrophages.

1980 ◽  
Vol 152 (5) ◽  
pp. 1236-1247 ◽  
Author(s):  
C A Rouzer ◽  
W A Scott ◽  
A L Hamill ◽  
Z A Cohn
Keyword(s):  
Time Course ◽  
Culture Medium ◽  
Peritoneal Macrophages ◽  
Experimental Conditions ◽  
Silicic Acid Chromatography ◽  
Pg Release ◽  
Antigen Antibody ◽  
Mouse Peritoneal Macrophages ◽  
Hydroxyeicosatetraenoic Acids

A method for the radiochemical assay of LTC production by mouse peritoneal macrophages in vitro is presented. The method involves labeling macrophages in culture with [5,6,8,9,11,12,14,15-3H]20:4 followed by stimulation of arachidonic acid (20:4) release under the experimental conditions desired. Radiolabeled leukotriene C (LTC) is recovered from the culture medium by extraction and silicic acid chromatography in 40% yield with full retention of biological activity. Because this LTC is radiochemically pure, the quantity of LTC release may be estimated from the amount of radioactivity in the sample. Use of the radioassay to study parameters affecting LTC synthesis by macrophages indicated that the time course of LTC synthesis and its relationship to the dose of a phagocytic stimulus (zymosan) were very similar to those of prostaglandin (PG) release. LTC release was also similar to that of PG in that lower levels of both metabolites were produced by Corynebacterium parvum-elicited macrophages than by resident cells. Finally, LTC release was stimulated in response to a challenge with antigen-antibody complexes, but lower maximal levels were attained than those with zymosan. The data presented here are consistent with the hypothesis that challenge of macrophages with a phagocytic stimulus leads to the release of 20:4 by an inducible phospholipase. Cyclooxygenase and lipoxygenase then compete for the released 20:4, leading to the production of PG, hydroxyeicosatetraenoic acids, and LTC.

scholarly journals The interaction in vitro of Pneumocystis carinii with macrophages and L-cells.

1978 ◽  
Vol 147 (1) ◽  
pp. 157-170 ◽  
Author(s):  
H Masur ◽  
T C Jones
Keyword(s):  
Pneumocystis Carinii ◽  
Significant Degree ◽  
Peritoneal Macrophages ◽  
Mononuclear Phagocytes ◽  
Humoral Factors ◽  
L Cells ◽  
Contrast Microscopy ◽  
Typical Morphology ◽  
Mouse Peritoneal Macrophages

A model was developed for studying the interaction between Pneumocystis, rat-derived cells, and humoral factors. Pneumocystis were obtained in large quantity by bronchial lavage of steroid-treated rats. The trophozoite was the predominant form obtained, and it could readily be recognized by phase contrast microscopy. Organisms maintained a typical morphology for at least 3 days in culture, and 10-20% took up radiolabeled nucleotides. Pneumocystis readily adhered to cell surfaces in a similar manner in alveolar macrophages from steroid-treated or normal rats, mouse peritoneal macrophages, and L-cells. Adherent organisms were not interiorized to a significant degree in the absence of antipneumocystis serum. After addition of rabbit antipneumocystis serum, rapid interiorization of organisms occurred from the surface of macrophages but not L-cells. Organisms appeared to be promptly destroyed within macrophages after interiorization. Persisting or multiplying intracellular forms were not seen. Antipneumocystis serum did not morphologically alter Pneumocystis. These observations suggest a role for antibody and mononuclear phagocytes during the immune response to Pneumocystis.

scholarly journals MECHANISMS OF ACQUIRED RESISTANCE IN MOUSE TYPHOID

1966 ◽  
Vol 124 (4) ◽  
pp. 585-600 ◽  
Author(s):  
R. V. Blanden ◽  
G. B. Mackaness ◽  
F. M. Collins
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Normal Mouse ◽  
Acquired Resistance ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Humoral Factors ◽  
Intraperitoneal Route ◽  
Mouse Peritoneal Macrophages

Experiments in vitro comparing normal mouse peritoneal macrophages with cells from Salmonella typhimurium-infected mice have shown that the "immune" macrophages have conspicuously enhanced microbicidal properties. Whereas normal macrophages could inactivate only 50 to 60% of intracellular S. typhimurium pretreated with immune serum, cells from infected animals killed virtually all ingested organisms and did so at an accelerated rate. Macrophages from Listeria monocytogenes-infected mice were shown to possess similarly enhanced microbicidal activity against S. typhimurium. Furthermore, the growth of S. typhimurium in the liver and spleen was more effectively restricted in Listeria-infected mice than in animals vaccinated with heat-killed S. typhimurium, even though the Listeria-infected animals possessed no demonstrable cross-reacting antibody to S. typhimurium. The lack of resistance in the mice vaccinated with heat-killed organisms could not be attributed to any deficiency of humoral factors, since the serum from these animals was as effective at promoting phagocytosis and killing by macrophages as serum from actively infected (and demonstrably resistant) mice. Conversely, Salmonella-infected mice were totally resistant to intravenous challenge with L. monocytogenes. The level of resistance in individual animals was related to the numbers of residual Salmonellae remaining in the tissues; mice with heavier residual infections being the more resistant. Specific antiserum from mice vaccinated with heat-killed S. typhimurium was found to be significantly protective only when the intraperitoneal route of challenge was employed. The foregoing studies have been interpreted to mean that enhancement of the microbicidal ability of macrophages is the mechanism of major importance in acquired resistance to S. typhimurium infection in mice.

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