scholarly journals OCCURRENCE OF TICK-BORNE PATHOGENS IN STRAY DOGS FROM HAVANA, CUBA

2018 ◽  
Vol 3 (4) ◽  
pp. 158-159
Author(s):  
B. G. Corona ◽  
A. A. Díaz-Sánchez ◽  
M. L. Meli ◽  
E. V. Cañizares ◽  
L. R. Arias ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Real Time ◽  
Real Time Pcr ◽  
Ixodid Tick ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
Rickettsia Felis ◽  
Stray Dogs

The article presents the results of examination of stray dogs from Havana, Cuba for six ixodid tick-borne diseases. Analysis was carried  out using real-time PCR. Overall 107 dogs, 95 (89.09 %) were infected.  41 dogs (38.31 %), 66 (61.68 %), 28 (26.17 %) and 40 (37.38 %)  were found to be infected with Anaplasma platys, Ehrlichia canis,  Rickettsia spp. and Hepatozoon canis, respectively. Sequence analysis of 16S rRNA and groEL genes for Rickettsia spp. revealed 99 % identity  with Rickettsia felis. There were no dogs infected with A. phagocytophilum and Borrelia spp.

scholarly journals Polyphasic characterization of Bacillus species from anthrax outbreaks in animals from South Africa and Lesotho

2016 ◽  
Vol 10 (08) ◽  
pp. 814-823 ◽  
Author(s):  
Kgaugelo Edward Lekota ◽  
Ayesha Hassim ◽  
Joseph Mafofo ◽  
Jasper Rees ◽  
Farai Catherine Muchadeyi ◽  
...  
Keyword(s):  
South Africa ◽  
Sequence Analysis ◽  
16S Rrna ◽  
Real Time ◽  
Real Time Pcr ◽  
Polyphasic Approach ◽  
Bacillus Species ◽  
Identification System ◽  
Conventional Pcr ◽  
Biolog System

Introduction: Bacillus anthracis is the causative agent of anthrax, a disease endemic in regions of Northern Cape Province and Kruger National Park of South Africa. Accurate identification of virulent B. anthracis is essential but challenging due to its close relationship with other members of B. cereus group. This study characterized B. anthracis and Bacillus species that were recovered from animals and the environment where animals died of anthrax symptoms in southern Africa using a polyphasic approach. Methodology: For this purpose, 3 B. anthracis and 10 Bacillus isolates were subjected to microbiology tests, BiologOmniLog identification system (Biolog), 16S ribosomal RNA (rRNA) sequence analysis, polymerase chain reaction (PCR) detection of protective antigen (pag) and capsule (cap) regions, and real-time PCR using hybridization probes targeting chromosomal, pag, and capC genes. Results: The Bacillus isolates were non-hemolytic, non-motile, and susceptible to penicillin, which is typical of B. anthracis, but resistant to gamma phage, unlike typical B. anthracis. The Biolog system and 16S rRNA gene sequence analysis identified most of the Bacillus isolates as B. endophyticus (7 of 10). Conventional PCR revealed that most of the Bacillus isolates contained capBCA gene regions. This highlights the limitation of the specificity of conventional PCR and the fact that the real-time PCR is more specific and reliable for anthrax diagnosis. Conclusions: Real-time PCR, 16S rRNA sequencing, and confirmatory microbiology tests including phage resistance distinguished Bacillus isolates from B. anthracis in this study. Identification of B. anthracis should be done using a polyphasic approach.

scholarly journals Molecular detection of pathogens in ticks and fleas collected from companion dogs and cats in East and Southeast Asia

2020 ◽  
Author(s):  
Viet-Linh Nguyen ◽  
Vito Colella ◽  
Grazia Greco ◽  
Fang Fang ◽  
Wisnu Nurcahyo ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Animal Species ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
Rickettsia Felis ◽  
Companion Dogs ◽  
Babesia Vogeli ◽  
East And Southeast Asia ◽  
Vector Borne

AbstractTicks and fleas are considered amongst the most important arthropod vectors of medical and veterinary concern due to their ability to transmit pathogens to a range of animal species including dogs, cats and humans. By sharing a common environment with humans, companion animal-associated parasitic arthropods may potentially transmit zoonotic vector-borne pathogens (VBPs). This study aimed to molecularly detect pathogens from ticks (n = 392) and fleas (n = 248) collected from companion dogs and cats in East and Southeast Asia. Of the 392 ticks tested, 37 (9.4%) scored positive for at least one pathogen with Hepatozoon canis being the most prevalent (5.4%), followed by Ehrlichia canis (1.8%), Babesia vogeli (1%), Anaplasma platys (0.8%) and Rickettsia spp. (1%) [including Rickettsia sp. (0.5%), Rickettsia asembonensis (0.3%), Rickettsia felis (0.3%)]. Out of 248 fleas tested, 106 (42.7%) were harboring at least one pathogen with R. felis being the most common (19.4%), followed by Bartonella spp. (16.5%), Rickettsia asembonensis (10.9%) and Candidatus Rickettsia senegalensis (0.4%). Furthermore, 35 Rhipicephalus sanguineus sensu lato ticks were subjected to phylogenetic analysis, of which 34 ticks belonged to the tropical and only one belonged to the temperate lineage (Rh. sanguineus sensu stricto). Our data reveals the circulation of different VBPs in ticks and fleas of dogs and cats from Asia, including zoonotic agents, which may represent a potential risk to animal and human health.Author summaryTicks and fleas are among the most important vectors of pathogens infesting many animal species including humans worldwide. Although a number of vector-borne pathogens have been detected in dogs and cats from East and Southeast Asia, investigation in ticks and fleas collected from them are scant. In order to provide an overview of the pathogens circulating in ticks and fleas from companion dogs and cats in Asia, ticks (n = 392) and fleas (n = 248) were collected from privately-owned dogs and cats from China, Taiwan, Indonesia, Malaysia, Singapore, Thailand, Philippines and Vietnam and molecularly screened for the presence of pathogens. Overall, multiple pathogens were found in ticks (i.e., Babesia vogeli, Hepatozoon canis, Ehrlichia canis, Anaplasma platys and Rickettsia spp.) and fleas (i.e., Rickettsia spp. and Bartonella spp.) from the sampling areas. Of the ticks tested, 9.4% scored positive for at least one pathogen and of fleas 42.7% harbored at least one pathogen with Rickettsia felis being the most common (19.4%). Overall, of the detected pathogens, R. felis stood out as the most important due to its zoonotic potential. The result of this study should increase awareness among pet owners and veterinary practitioners regarding the importance of ticks and fleas, and their transmitted pathogens.

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR

2021 ◽  
pp. 81-86
Author(s):  
Marcus Beissner ◽  
Richard Odame Phillips ◽  
Gisela Bretzel
Keyword(s):  
16S Rrna ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr

Monitoring of Microbial Community Inhabiting a Low-Grade Copper Sulphide Ore by Quantitative Real-Time PCR Analysis of 16S rRNA Genes

2007 ◽  
Vol 20-21 ◽  
pp. 539-542 ◽  
Author(s):  
Francisco Remonsellez ◽  
F. Galleguillos ◽  
Sonestie Janse van Rensburg ◽  
G.F. Rautenbach ◽  
Pedro A. Galleguillos ◽  
...  
Keyword(s):  
16S Rrna ◽  
Real Time ◽  
Real Time Pcr ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Low Grade ◽  
Copper Sulphide ◽  
Quantitative Real Time Pcr ◽  
Sulphide Ore ◽  
Heap Bioleaching

Microbial heap bioleaching is being used as an industrial process to recover copper from low grade ores. It is known that a consortium of different microorganisms participates in this process. Therefore identification and quantification of communities inhabiting heap bioleaching operations is a key step for understanding the dynamics and role of these microorganisms in the process. A quantitative real-time PCR approach was used to investigate the microbial dynamics in this process. To study the microbial population inhabiting a low-grade copper sulphide ore bioleaching industrial heap process at Escondida Mine in Chile, 16S rRNA genetic libraries were constructed using bacterial and archaeal universal primers. Phylogenetic analyses of sequences retrieved from genetic libraries showed that the community is mainly composed by microoganisms related to Acidithiobacillus ferrooxidans (2 strains), Acidithiobacillus thiooxidans, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and the archaea Ferroplasma. Specific primers for real-time PCR determination were designed and tested to amplify each of the sequences obtained by cloning. Standard curves for real time PCR were performed using plasmid DNA from selected clones. This methodology is actually being used to monitor relevant microorganisms inhabiting this low-grade copper sulphide ore bioleaching industrial heap.

Shell-vial culture and real-time PCR applied to Rickettsia typhi and Rickettsia felis detection

2015 ◽  
Vol 31 (11) ◽  
pp. 1747-1754 ◽  
Author(s):  
Ferran Segura ◽  
Immaculada Pons ◽  
Júlia Pla ◽  
María-Mercedes Nogueras
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Shell Vial

Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia

2008 ◽  
Vol 97 (10) ◽  
pp. 1376-1380 ◽  
Author(s):  
Andreas Ohlin ◽  
Anders Bäckman ◽  
Maria Björkqvist ◽  
Paula Mölling ◽  
Margaretha Jurstrand ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Real Time ◽  
Real Time Pcr ◽  
Rrna Gene ◽  
The 16S Rrna Gene
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