scholarly journals The effect of 17% ethylenediaminetetraacetic acid and 10% citric acid solutions with manual and ultrasonic agitation on smear layer removal and intraradicular dentine erosion: a scanning electron microscope evaluation

2020 ◽  
Vol 47 (1) ◽  
pp. 7-13
Author(s):  
Alessandro Rodrigo MAGGIONI ◽  
Helena Rosa Campos RABANG ◽  
Brenda Paula Figueiredo De Almeida GOMES ◽  
Luiza Gonçalves ROMA ◽  
Isabel Coelho Gomes CAMÕES
Author(s):  
Dasarapu Srujan ◽  
Gaurav Malhotra ◽  
Pradeep Shukla ◽  
Prerna Kataria ◽  
Mona Dagar

Background: Smear layer removal and collagen fiber exposure may improve regeneration which can be accomplished by use of root biomodifiers. These enhance the degree of connective tissue attachment to denuded roots. The objective of this in vitro study was to evaluate and compare novel root canal irrigant and other root biomodifiers for smear layer removal on periodontally involved human teeth. Materials and Methods: Forty human teeth were collected and stored in saline. After scaling and root planing, two samples were obtained from each tooth. A total of 100 dentin blocks were randomly divided into four groups: Mixture of tetracycline, acid and detergent (MTAD), tetracycline hydrochloride (TTC HCl), citric acid (CA), and ethylenediaminetetraacetic acid (EDTA). The agents were applied for 3 min by active burnishing. Immediately following treatment, the specimens were rinsed, dehydrated, fixed and prepared for scanning electron microscope and were examined at × 3500 magnification. Sampaio’s index was evaluated by the previously trained blind examiner using photomicrographs.  Groups  were  compared  using  analysis  of  variance  followed  by  Tukey’s  post?hoc  test. Results: Mixture of tetracycline, acid, and detergent is most efficacious in removing the smear layer and showed statistically significant dentinal tubules opening, followed by EDTA, TTC HCl, and CA. Conclusion: Mixture of tetracycline, acid and detergent and conventional root biomodifiers used in the study alters the dentin surface by smear layer removal and exposure of dentinal tubules. Hence, MTAD as a root biomodifier may have a significant role in periodontal regeneration. Key words: Citric acid, ethylenediaminetetraacetic acid, MTAD, root biomodifier, scanning electron microscope study, tetracycline hydrochloride


2014 ◽  
Vol 02 (03) ◽  
pp. 138-144
Author(s):  
Atul Jain ◽  
Sonal Sinha ◽  
Rachana Bahuguna ◽  
Neeraj Kumar ◽  
Raju Chauhan

Abstract Aim: This study was carried out to evaluate the combined effect of taper and different irrigating agents on the cleanliness of root canal. Materials & Methods: 45 extracted mandibular premolars were divided into three groups, according to the taper produced, 2%, 4% and 6% with Hero Shaper files respectively, which were further divided into subgroups, according to the irrigation regime followed. The teeth were split and subjected to SEM analysis. Results: The results showed that the canals prepared to a taper of 6% and irrigated with 5.2% NaOCl and 25% Citric acid had less debris and smear layer, which was statistically significant. Conclusion: It was concluded that a taper of 6% facilitates irrigant delivery in the canal and 25% Citric acid is more potent demineralising agent than 17% EDTA.


2008 ◽  
Vol 34 (11) ◽  
pp. 1396-1400 ◽  
Author(s):  
Alessandra Sverberi Carvalho ◽  
Carlos Henrique Ribeiro Camargo ◽  
Marcia Carneiro Valera ◽  
Samira Esteves Afonso Camargo ◽  
Maria Nadir Gasparoto Mancini

2017 ◽  
Vol 18 (12) ◽  
pp. 1135-1143
Author(s):  
Prem P Kar ◽  
Sandhya A Khasnis ◽  
Krisnamurthy H Kidiyoor

ABSTRACT Aim The aim of the study was to compare the cleaning efficacy (debris and smear layer removal) of two multifile rotary systems (MTwo and Silk) and two single-file rotary systems (F6 Skytaper and NeoNiTi). Materials and methods Eighty mesial canals of mandibular first molars were cleaned and shaped using four nickel–titanium (NiTi) rotary instruments to size # 25 and 3% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA). Samples were randomly divided into four equal groups (n = 20) according to instrumentation: Group I, Mtwo; group II, Silk; group III, F6 Skytaper; group IV, NeoNiTi. Samples were split longitudinally and examined under scanning electron microscope (SEM) for debris and smear layer removal in coronal, middle, and apical thirds of each root canal. Results F6 skytaper and Mtwo groups showed significantly higher debris removal than Silk and NeoNiTi groups in apical third of root canal as well as when compared with NeoNiTi group in middle third. F6 Skytaper group showed significantly higher debris and smear layer removal than Silk group in coronal third. There was statistically significant difference among all thirds of root canal in terms of debris removal in Silk and NeoNiTi groups. There was statistically significant difference among all thirds of root canal in F6 Skytaper and NeoNiTi groups in terms of smear layer removal. Conclusion F6 Skytaper single-file rotary instrumentation showed the maximum cleaning efficacy followed by Mtwo multifile rotary instrumentation in all thirds of root canal. Clinical significance F6 Skytaper rotary instrument is most efficient followed by Mtwo rotary instrument among all rotary instruments. How to cite this article Kar PP, Khasnis SA, Kidiyoor KH. Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel-titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study. J Contemp Dent Pract 2017;18(12):1135-1143.


2021 ◽  
Vol 11 (24) ◽  
pp. 11976
Author(s):  
Mohannad Nassar ◽  
Md. Sofiqul Islam ◽  
Smriti Aryal A C ◽  
Hatem Mostafa El-Damanhoury ◽  
Salvatore Sauro ◽  
...  

Phytic acid (IP6) has the ability to etch tooth structure due to its acidity and chelating properties. This study aimed to evaluate the micro-tensile bond strength (μTBS) of resin-based cement to enamel and dentin using manufacturer-provided etchants, phosphoric acid (PA) for enamel and a mixture of 10% citric acid and 3% ferric chloride (CAF) for dentin and compare them to IP6. Enamel surfaces were etched for 30 s with either PA or IP6 (1%, 5% or 10%). Dentin surfaces were etched for 15 s with either CAF or 1% IP6. Pre-polymerized composite blocks were bonded to enamel and dentin surfaces. After 24 h, the specimens were sectioned into beams and subjected to μTBS testing. De-bonded specimens and smear layer removal were analyzed with a scanning electron microscope. Etching enamel with 1% and 5% IP6 resulted in μTBS values that are comparable to the control (PA) (p > 0.05), while 10% IP6 negatively affected the μTBS (p < 0.05). The μTBS to dentin etched with 1% IP6 was higher compared to the control (CAF) (p = 0.001). IP6 effectively removed enamel and dentin smear layer, and at specific concentrations, it may be a suitable alternative conditioner agent for bonding resin-based cement to enamel and dentin.


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