scholarly journals Characterization of Novel Cellulosome Complex of Clostridium cellulovorans TCW from Coffee Pulp Waste

2020 ◽  
Vol 107 (Special) ◽  
Keyword(s):  
Clostridium Cellulovorans ◽  
Coffee Pulp ◽  
Coffee Pulp Waste

scholarly journals Characterisation of Arabica Coffee Pulp - Hay from Kintamani - Bali as Prospective Biogas Feedstocks

2018 ◽  
Vol 164 ◽  
pp. 01039 ◽  
Author(s):  
Roy Hendroko Setyobudi ◽  
Satriyo Krido Wahono ◽  
Praptiningsih Gamawati Adinurani ◽  
Ahmad Wahyudi ◽  
Wahyu Widodo ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Raw Materials ◽  
Raw Material ◽  
Arabica Coffee ◽  
Coffee Pulp ◽  
Preservation Technology ◽  
Volatile Solids ◽  
Coffee Pulp Waste ◽  
Alternative Solutions

The huge amount of coffee pulp waste is an environmental problem. Anaerobic fermentation is one of the alternative solutions. However, availability of coffee pulp does not appear for year-round, whereas biogas needs continuous feedstocks for digester stability. This research uses coffee pulp from Arabica Coffee Factory at Mengani, Kintamani, Bali–Indonesia. The coffee pulp was transformed into coffee pulp-hay product by sun drying for preservations to extend the raw materials through the year. Characterization of coffee pulp-hay was conducted after to keep for 15 mo for review the prospect as biogas feedstocks. Several parameters were analyzed such as C/N ratio, volatile solids, carbohydrate, protein, fat, lignocellulose content, macro-micro nutrients, and density. The review results indicated that coffee pulp-hay is prospective raw material for biogas feedstock. This well-proven preservation technology was able to fulfill the continuous supply. Furthermore, some problems were found in the recent preliminary experiment related to the density and fungi growth in the conventional laboratory digester. Further investigation was needed to implement the coffee pulp – hay as biogas feedstocks.

Structural characterization of native and oxidized procyanidins (condensed tannins) from coffee pulp (Coffea arabica) using phloroglucinolysis and thioglycolysis-HPLC-ESI-MS

2021 ◽  
Vol 340 ◽  
pp. 127830
Author(s):  
Jorge E. Wong-Paz ◽  
Sylvain Guyot ◽  
Pedro Aguilar-Zárate ◽  
Diana B. Muñiz-Márquez ◽  
Juan C. Contreras-Esquivel ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Coffea Arabica ◽  
Condensed Tannins ◽  
Coffee Pulp ◽  
Esi Ms

Evaluation of coffee pulp as substrate for polygalacturonase production in solid state fermentation

2020 ◽  
pp. 117
Author(s):  
Rosa Amarilis Rodríguez Frómeta ◽  
José Laencina Sánchez ◽  
José María Ros García
Keyword(s):  
Cell Wall ◽  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Growth Substrate ◽  
Optimal Temperature ◽  
Coffee Pulp ◽  
Polygalacturonase Production ◽  
Optimal Ph

The purpose of this research is to evaluate the coffee pulp, a by-product of coffee processing, as substrate for polygalacturonase production by solid state fermentation. In addition, it is a way to take advantage of the coffee pulp. Characterization of the coffee pulp revealed a high content of nutrients for fungi growth, such as reducing sugars (5.4% of dry pulp), proteins (9.4% of dry pulp), pectins (20.5% of dry pulp), which are inducers of pectic hydrolases production and source of carbon after degradation, and caffeine (1.4% of dry pulp), among others. The characterization of the cell-wall of coffee pulp revealed, after polysaccharides fractionation, the content in cell-wall pectins (25.5%), hemicelluloses (11.5%) and cellulosic residue (44%). A strain of Aspergillus niger, called van Thiegem, has been selected as a good producer of polygalacturonases (60 U/ml) using the coffee pulp as the growth substrate. During fermentation, reducing sugars, caffeine and phenolic compounds were consumed till almost exhaustion. A partial characterization of the polygalacturonase using high methoxyl pectin as substrate indicates an optimal pH of 4.0 and 45oC as optimal temperature, which are good values for the use of the enzyme in vegetable processing, including coffee processing.

A study on bioethanol production from cashew apple pulp and coffee pulp waste

2011 ◽  
Vol 35 (10) ◽  
pp. 4107-4111 ◽  
Author(s):  
Deepa Shenoy ◽  
Anjali Pai ◽  
R.K. Vikas ◽  
H.S. Neeraja ◽  
J.S. Deeksha ◽  
...  
Keyword(s):  
Bioethanol Production ◽  
Coffee Pulp ◽  
Cashew Apple ◽  
Coffee Pulp Waste

Acid hydrolysis and carbohydrates characterization of coffee pulp

1996 ◽  
Vol 9 (1-4) ◽  
pp. 1041-1044 ◽  
Author(s):  
G. Urbaneja ◽  
J. Ferrer ◽  
G. Paez ◽  
L. Arenas ◽  
G. Colina
Keyword(s):  
Acid Hydrolysis ◽  
Coffee Pulp

scholarly journals Optimization of protease production from surface-modified coffee pulp waste and corncobs using Bacillus sp. by SSF

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Selvam Kandasamy ◽  
Govarthanan Muthusamy ◽  
Senthilkumar Balakrishnan ◽  
Senbagam Duraisamy ◽  
Selvankumar Thangasamy ◽  
...  
Keyword(s):  
Protease Production ◽  
Bacillus Sp ◽  
Coffee Pulp ◽  
Coffee Pulp Waste ◽  
Surface Modified

scholarly journals The Effect of Mixed Biological Pretreatment and PEG 4000 on Reducing Sugar Production from Coffee Pulp Waste

2019 ◽  
Vol 10 (3) ◽  
pp. 453
Author(s):  
Toto Iswanto ◽  
Nuniek Hendrianie ◽  
Maya Shovitri ◽  
Ali Altway ◽  
Tri Widjaja
Keyword(s):  
Reducing Sugar ◽  
Sugar Production ◽  
Biological Pretreatment ◽  
Coffee Pulp ◽  
Peg 4000 ◽  
Coffee Pulp Waste

scholarly journals Characterization of Xylanolytic Enzymes in Clostridium cellulovorans: Expression of Xylanase Activity Dependent on Growth Substrates

2001 ◽  
Vol 183 (24) ◽  
pp. 7037-7043 ◽  
Author(s):  
Akihiko Kosugi ◽  
Koichiro Murashima ◽  
Roy H. Doi
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Xylanase Activity ◽  
Cellulolytic Bacterium ◽  
Clostridium Cellulovorans ◽  
Molecular Weights ◽  
Chromatography Analysis ◽  
Layer Chromatography ◽  
Activity Dependent

ABSTRACT Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions, the activity was present in both fractions. Each of these fractions contained at least two major and three minor xylanase activities. In both fractions, the pattern of xylan hydrolysis products was almost identical based on thin-layer chromatography analysis. The major xylanase activities in both fractions were associated with proteins with molecular weights of about 57,000 and 47,000 according to zymogram analyses, and the minor xylanases had molecular weights ranging from 45,000 to 28,000. High α-arabinofuranosidase activity was detected exclusively in the noncellulosomal fraction. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that cellulosomes derived from xylan-, cellobiose-, and cellulose-grown cultures had different subunit compositions. Also, when xylanase activity in the cellulosomes from the xylan-grown cultures was compared with that of cellobiose- and cellulose-grown cultures, the two major xylanases were dramatically increased in the presence of xylan. These results strongly indicated that C. cellulovorans is able to regulate the expression of xylanase activity and to vary the cellulosome composition depending on the growth substrate.

scholarly journals Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A.

1993 ◽  
Vol 175 (18) ◽  
pp. 5762-5768 ◽  
Author(s):  
M A Goldstein ◽  
M Takagi ◽  
S Hashida ◽  
O Shoseyov ◽  
R H Doi ◽  
...  
Keyword(s):  
Binding Protein ◽  
Protein A ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Clostridium Cellulovorans ◽  
Cellulose Binding
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