Screening of Piper Species for Resistance to Quick Wilt caused by Phytophthora capsici under Glasshouse Condition

2019 ◽  
Vol 106 (1-3) ◽  
Author(s):  
M. Chinnapappa ◽  
A. Ramar ◽  
L. Pugalendhi ◽  
P. Muthulakshmi ◽  
P. Vetrivelkalai
Keyword(s):  
Phytophthora Capsici ◽  
Glasshouse Condition

scholarly journals Facile Synthesis of Carboxymethyl Cellulose Coated Core/Shell SiO2@Cu Nanoparticles and Their Antifungal Activity against Phytophthora capsici

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 888
Author(s):  
Nguyen Thi Thanh Hai ◽  
Nguyen Duc Cuong ◽  
Nguyen Tran Quyen ◽  
Nguyen Quoc Hien ◽  
Tran Thi Dieu Hien ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Carboxymethyl Cellulose ◽  
Chemical Reduction ◽  
Low Cost ◽  
Phytophthora Capsici ◽  
Reduction Process ◽  
Spherical Shape ◽  
Core Shell ◽  
Cu Nanoparticles ◽  
Average Size

Cu nanoparticles are a potential material for creating novel alternative antimicrobial products due to their unique antibacterial/antifungal properties, stability, dispersion, low cost and abundance as well as being economical and ecofriendly. In this work, carboxymethyl cellulose coated core/shell SiO2@Cu nanoparticles (NPs) were synthesized by a simple and effective chemical reduction process. The initial SiO2 NPs, which were prepared from rice husk ash, were coated by a copper ultrathin film using hydrazine and carboxymethyl cellulose (CMC) as reducing agent and stable agent, respectively. The core/shell SiO2@Cu nanoparticles with an average size of ~19 nm were surrounded by CMC. The results indicated that the SiO2@Cu@CMC suspension was a homogenous morphology with a spherical shape, regular dispersion and good stability. Furthermore, the multicomponent SiO2@Cu@CMC NPs showed good antifungal activity against Phytophthora capsici (P. capsici). The novel Cu NPs-based multicomponent suspension is a key compound in the development of new fungicides for the control of the Phytophthora disease.

scholarly journals Physiological and Biochemical Responses of Ageratum conyzoides, Oryza sativa f. spontanea (Weedy Rice) and Cyperus iria to Parthenium hysterophorus Methanol Extract

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1205
Author(s):  
Mst. Motmainna ◽  
Abdul Shukor Juraimi ◽  
Md. Kamal Uddin ◽  
Norhayu Binti Asib ◽  
A. K. M. Mominul Islam ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Methanol Extract ◽  
Parthenium Hysterophorus ◽  
Carotenoid Content ◽  
Weedy Rice ◽  
Ageratum Conyzoides ◽  
Test Species ◽  
Biochemical Responses ◽  
Glasshouse Condition ◽  
Physiological And Biochemical

The current study was designed to investigate the effect of Parthenium hysterophorus L. methanol extract on Ageratum conyzoides L., Oryza sativa f. spontanea (weedy rice) and Cyperus iria L. in glasshouse condition. Here, Parthenium hysterophorus methanol extract at 20, 40, and 60 g L−1 concentrations was applied on the test species to examine their physiological and biochemical responses at 6, 24, 48 and 72 h after spraying (HAS). The phytotoxicity of P. hysterophorus was strong on A. conyzoides compared to weedy rice and Cyperus iria at different concentrations and exposure times. There was a reduction in photosynthesis rate, stomatal conductance, transpiration, chlorophyll content and carotenoid content when plants were treated with P. hysterophorus extract concentrations. Exposure to P. hysterophorus (60 g L−1) at 24 HAS increased malondialdehyde (MDA) and proline content by 152% and 130%, respectively, in A. conyzoides compared with control. The activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) were also increased in the presence of P. hysterophorus extract. Present findings confirm that the methanol extract of P. hysterophorus can disrupt the physiological and biochemical mechanism of target weeds and could be used as an alternative to chemical herbicides.

scholarly journals A Genome-Wide Analysis of Pathogenesis-Related Protein-1 (PR-1) Genes from Piper nigrum Reveals Its Critical Role during Phytophthora capsici Infection

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1007
Author(s):  
Divya Kattupalli ◽  
Asha Sreenivasan ◽  
Eppurathu Vasudevan Soniya
Keyword(s):  
Phytophthora Capsici ◽  
Regulatory Elements ◽  
Piper Nigrum ◽  
Binding Motif ◽  
Altered Expression ◽  
Genome Wide ◽  
A Genome ◽  
Pathogenesis Related Protein ◽  
Pathogenesis Related

Black pepper (Piper nigrum L.) is a prominent spice that is an indispensable ingredient in cuisine and traditional medicine. Phytophthora capsici, the causative agent of footrot disease, causes a drastic constraint in P. nigrum cultivation and productivity. To counterattack various biotic and abiotic stresses, plants employ a broad array of mechanisms that includes the accumulation of pathogenesis-related (PR) proteins. Through a genome-wide survey, eleven PR-1 genes that belong to a CAP superfamily protein with a caveolin-binding motif (CBM) and a CAP-derived peptide (CAPE) were identified from P. nigrum. Despite the critical functional domains, PnPR-1 homologs differ in their signal peptide motifs and core amino acid composition in the functional protein domains. The conserved motifs of PnPR-1 proteins were identified using MEME. Most of the PnPR-1 proteins were basic in nature. Secondary and 3D structure analyses of the PnPR-1 proteins were also predicted, which may be linked to a functional role in P. nigrum. The GO and KEGG functional annotations predicted their function in the defense responses of plant-pathogen interactions. Furthermore, a transcriptome-assisted FPKM analysis revealed PnPR-1 genes mapped to the P. nigrum-P. capsici interaction pathway. An altered expression pattern was detected for PnPR-1 transcripts among which a significant upregulation was noted for basic PnPR-1 genes such as CL10113.C1 and Unigene17664. The drastic variation in the transcript levels of CL10113.C1 was further validated through qRT-PCR and it showed a significant upregulation in infected leaf samples compared with the control. A subsequent analysis revealed the structural details, phylogenetic relationships, conserved sequence motifs and critical cis-regulatory elements of PnPR-1 genes. This is the first genome-wide study that identified the role of PR-1 genes during P. nigrum-P. capsici interactions. The detailed in silico experimental analysis revealed the vital role of PnPR-1 genes in regulating the first layer of defense towards a P. capsici infection in Panniyur-1 plants.

scholarly journals Characteristic of the Pepper CaRGA2 Gene in Defense Responses against Phytophthora capsici Leonian

2013 ◽  
Vol 14 (5) ◽  
pp. 8985-9004 ◽  
Author(s):  
Ying-Li Zhang ◽  
Qing-Li Jia ◽  
Da-Wei Li ◽  
Jun-E Wang ◽  
Yan-Xu Yin ◽  
...  
Keyword(s):  
Phytophthora Capsici ◽  
Defense Responses

scholarly journals A PCR-based Assay for Distinguishing between A1 and A2 Mating Types of Phytophthora capsici

2017 ◽  
Vol 142 (4) ◽  
pp. 260-264
Author(s):  
Ping Li ◽  
Dong Liu ◽  
Min Guo ◽  
Yuemin Pan ◽  
Fangxin Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mating Type ◽  
Phytophthora Capsici ◽  
Mating Types ◽  
Sequence Repeat ◽  
Chain Reaction ◽  
Plant Parasite ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Microsatellite Diversity

Sexual reproduction in the plant parasite Phytophthora capsici Leonian requires the interaction of two distinct mating types, A1 and A2. Co-occurrence of these mating types can enhance the genetic diversity of P. capsici and alter its virulence or resistance characteristics. Using an intersimple sequence repeat (ISSR) screen of microsatellite diversity, we identified, cloned, and sequenced a novel 1121-base pair (bp) fragment specific to the A1 mating type of P. capsici. Primers Pcap-1 and Pcap-2 were designed from this DNA fragment to specifically detect the A1 mating type. Polymerase chain reaction (PCR) using these primers amplified an expected 997-bp fragment from known A1 mating types, but yielded a 508-bp fragment from known A2 mating types. This PCR-based assay could be adapted to accurately and rapidly detect the co-occurrence of A1 and A2 P. capsici mating types from field material.

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