scholarly journals Evaluation of Ethyl Acetate Extract of Some Botanicals against Rice Weevil Sitophilus oryzae L. (Coleoptera: Curculionidae) in Stored Maize

2021 ◽  
Vol 108 (june) ◽  
pp. 1-6
Author(s):  
Anandhabhairavi N ◽  
◽  
Shanthi M ◽  
Chinniah C ◽  
Geetha R ◽  
...  

In the present study, botanicals (11 nos.) were extracted using ethyl acetate solvent (mid-polar) and evaluated for their fumigant toxicity and repellent activity at 5% concentration against rice weevil, Sitophilus oryzae L. in comparison with Acorus calamus.The results revealed that all the botanicals were effective against Sitophilus oryzae compared to untreated control. Mentha spicata 5% ethyl acetate extract exhibited 83.33% fumigant toxicity after 72 hours of treatment with maximum repellency rate of 76.11%. Ocimum sanctum displayed 80.00% fumigant toxicity with the repellency rate of 76.11% and grouped under Class IV. Vitex negundo 5% treatment caused 83.33% fumigant toxicity, and it was statistically on par with the M. spicata and the repellency rate was 72.78 against S. oryzae. Curcuma longa caused effective repellency rate of 75.56% and was grouped under Class IV. Hence, it is concluded that 5% ethyl acetate extract of M. spicata, V. negundo and O.sanctum were toxic against S. oryzae in stored maize.

2001 ◽  
Vol 20 (4) ◽  
pp. 317-320 ◽  
Author(s):  
Byung-Ho Lee ◽  
Won-Sik Choi ◽  
Sung-Eun Lee ◽  
Byeoung-Soo Park

Author(s):  
Ofem Eteng ◽  
C.A. MOSES ◽  
J. ENOBONG ◽  
A.J. AKAMO ◽  
D.I. AKINLOYE ◽  
...  

Abstract. Eteng OE, Moses CA, Enobong J, Akamo AJ, Akinloye DI, Ugbaja RN, Akinloye OA. 2020. Protective effects of Curcuma longa rhizomes ethyl acetate extract against alcohol induced oxidative stress and nephrotoxicity in female Wistar rats. Biofarmasi J Nat Prod Biochem 21: 5-12. This study aimed to evaluate the protective effect of Curcuma longa Linn. (syn. Curcuma domestica Val.) rhizomes ethyl acetate extract (CLREAE) facing alcohol-induced oxidative stress and nephrotoxicity. Thirty female (30) Wistar rats were categorized randomly into six groups. Groups 1, 2, 3, 4, 5 and 6 were treated with normal saline; 20% ethanol; 100 mg of CLREAE + 20% ethanol; 200 mg of CLREAE + 20%; 350 mg of CLREAE + 20% ethanol and 350 mg of CLREAE respectively for 14 days. A significant (p<0.05) decrease in the SOD, CAT and GPx activities and GSH concentration of rat treated with only 20% ethanol were found when compared to the normal control group, whereas a significant (P<0.05) increase in the groups pretreated with different doses of the CLREAE were also found when compared to groups with only 20% ethanol treatment. Thus, comparing to the normal control group, treatment with the CLREAE fetched a significant (p<0.05) decrease in the renal biomarkers (creatinine and urea).  Whilst, comparing to the groups with 20% methanol treatment, a significant (p<0.05) increase happened in the groups pretreated with different doses of the CLREAE. There was a significant (p<0.05) decrease on Kidney MDA level in rats pretreated with different doses of CLREAE compared with the normal control. It was shown in the results of the histology that there was a physiologic recovery in the kidney tissues as groups were treated with different doses of the CLREAE. Evidenced by reduced necrosis of tubular and glomerular epithelial, the signs of protection against toxicity were found on the rats. The study suggested that through in vivo free radical scavenging ability, the CLREAE has protective effects against alcohol-induced oxidative stress and nephrotoxicity in female Wistar rats.


2001 ◽  
Vol 57 (6) ◽  
pp. 548-553 ◽  
Author(s):  
Sung-Eun Lee ◽  
Byoung-Ho Lee ◽  
Won-Sik Choi ◽  
Byeoung-Soo Park ◽  
Jeong-Gyu Kim ◽  
...  

Molecules ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 361 ◽  
Author(s):  
Sung-Woong Kim ◽  
Hyo-Rim Lee ◽  
Myeong-Jin Jang ◽  
Chan-Sik Jung ◽  
Il-Kwon Park

2017 ◽  
Vol 4 (9) ◽  
pp. 1574 ◽  
Author(s):  
Karishma Agarwal ◽  
Dhananjay Kumar Singh ◽  
Jyotshna Jyotshna ◽  
Ateeque Ahmad ◽  
Karuna Shanker ◽  
...  

Background: Ocimum sanctum and Ocimum kilimandscharicum are cultivated in Indian subcontinent both for the religious and medicinal properties. Traditionally, the leaves have been reported for their enormous therapeutic potentials but the roots which are otherwise considered as a waste part have not been explored for their pharmacological activity. Methods: Total phenolic content, free radical scavenging and ferric reducing antioxidant potential of various extracts from Ocimum sanctum and Ocimum kilimandscharicum were assessed and compared. In vitro antioxidant activity was estimated spectrophotometrically and the most potent ethyl acetate extract was chemically characterized by developing the chemical fingerprint and quantifying the probable constituents with the help of HPLC/LC-MS techniques. Results: The ethyl acetate extract of both the species exhibited significant free radical scavenging potential and also reduced the ferric ions. It was observed that ethyl acetate extract have superior ferric reducing potential than other tested extracts, which were evidenced by high ferrous sulphate equivalent value of 77.05 ± 1.54 and 80.98 ± 0.80 at 100 µg/ml for O. sanctum and O. kilimandscharium respectively. The ferric reducing capacity of ethyl acetate extract for both the species was also evidenced by an elevated optical density of 1.64 ± 0.12 and 2.14 ± 0.08. Ocimum sanctum exhibited better antioxidant capacity (11.31 ± 0.20 AScE) as compared to Ocimum kilimandscharium (9.08 ± 0.27 AScE). The total phenolic and flavonoid content were estimated by spectrophotometric method and tentatively characterized by HPLC/LC-MS profiling which revealed the presence of rosmarinic acid, caffeic acid along with its derivatives such as caffeoyl-dihydroxyphenyllactoyl-tartaric acid. Conclusion: The ethyl acetate extract of both the species being rich in phenolic and flavonoid contents exhibited potent antioxidant activity. The presence of flavonoid in ethyl acetate extracts further co-relates the antioxidative properties of roots extracts.  To the best of our knowledge and understanding this is the first report of comparative chemical profiling by RP-HPLC/LC-MS and antioxidant potential of roots of two Ocimum species. Peer Review Details Peer review method: Single-Blind (Peer-reviewers: 02) Peer-review policy Plagiarism software screening?: Yes Date of Original Submission: 27 July 2017 Date accepted: 23 August 2017 Peer reviewers approved by: Dr. Lili Hami Editor who approved publication: Dr. Phuc Van Pham  


2017 ◽  
Vol 107 (4) ◽  
pp. 543-549 ◽  
Author(s):  
T. Aydin ◽  
N. Bayrak ◽  
E. Baran ◽  
A. Cakir

AbstractInsecticidal effects of the dichloromethane, ethyl acetate, acetone, ethanol and methanol extracts of Humulus lupulus (hops) L. cones and its principal components, xanthohumol was investigated on five stored pests, Sitophilus granarius (L.), Sitophilus oryzae (L.), Acanthoscelides obtectus (Say.), Tribolium castaneum (Herbst) and Lasioderma serricorne (F.). The mortality of adults of the insects treated with 2, 5, 5, 10 and 20 mg ml̠−1 concentrations of the extracts and xanthuhumol was counted after 24, 48, 72, 96 and 120 h. In order to determine the toxic effects of the substances tested against all tested insects, durations for 50% mortality of the adults, and LD50 values were also determined in the first 48 h by probit analysis. Our results also showed that xanthohumol was more toxic against the pests in comparison with the extracts applications. LD50 values for xanthohumol were found to be low dose as compared with the extracts. Xanthohumol was more toxic against S. granarius (L.) with 6.8 µg of LD50 value. Among the extracts, methanol extract was less effective than other extracts against all tested insects. The ethyl acetate extract of H. lupulus cones was the most effective extract against the tested pests. The quantitative amounts of xanthohumol in the extracts were determined using a high-performance liquid chromatography. The quantitative data indicated that amount of xanthohumol in the extracts increased with increase of polarity of the solvents used from methanol to dichloromethane. The methanol extract contained the high amount of xanthohumol with 5.74 g/100 g extract (0.46 g/100 g plant sample).


Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
RM Marçal ◽  
DM Ptak ◽  
RR Krempser ◽  
MR Krempser ◽  
SV Floresta ◽  
...  

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