scholarly journals Protective Effects of Cherry Extract on Malondialdehyde Levels, Catalase Activity, and Edema Induced by Middle Cerebral Artery Occlusion in a Rat Stroke Model

2020 ◽  
Vol 8 (3) ◽  
pp. 1-9
Author(s):  
Firoozeh Alavian ◽  
◽  
Kimia Alavian ◽  
Saeedeh Ghiasvand ◽  
Leila Rezaeian ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Catalase Activity ◽  
Artery Occlusion ◽  
Protective Effects ◽  
Stroke Model ◽  
Cerebral Artery Occlusion

Melatonin and calpeptin synergistically protect against post-reperfusion injury in a rat middle cerebral artery occlusion stroke model

2021 ◽  
Vol 4 (4) ◽  
pp. 592-612
Author(s):  
Ye Feng ◽  
Qian Xu ◽  
Raymond Tak Fai Cheung
Keyword(s):  
Cerebral Ischemia ◽  
Middle Cerebral Artery ◽  
Reperfusion Injury ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Neurological Deficit ◽  
Infarct Volume ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Cerebral Artery Occlusion

Cerebral ischemia induces oxidative injury and increases the intracellular calcium ion concentration to activate several calcium-dependent proteases such as calpains. Calpain activation leads to various necrotic and apoptotic processes. Calpeptin is a potent, cell-permeable calpain inhibitor. As a strong antioxidant and free radical scavenger, melatonin shows beneficial effect in rodent models of focal cerebral ischemia when given prior to ischemia or reperfusion. This study was focused on the neuroprotective effects of melatonin and/or calpeptin given after onset of reperfusion. For this purpose, right-sided middle cerebral artery occlusion (MCAO) for 90 minutes followed by 24 or 72 hours of reperfusion was performed in male Sprague Dawley rats, then, melatonin 50 or 150 µg/kg, calpeptin 10, 15 or 50 µg/kg or a combination of melatonin 50 µg/kg plus calpeptin 15 or 50 µg/kg were injected via an intracerebroventricular route at 15 minutes after onset of reperfusion. Melatonin or calpeptin tended to reduce the relative infarct volume and significantly decreased the neurological deficit at 24 hours. The combination achieved a greater protection than each of them alone. Melatonin, calpeptin or the combination all decreased Fluoro-Jade B (FJB)+ degenerative neurons and cleaved/total caspase-3 ratio at 24 hours. These treatments did not significantly impact the density of surviving neurons and ED-1+ macrophage/activated microglia. At the 72-hour-reperfusion, melatonin or the combination decreased the relative infarct volume and neurological deficit. Nevertheless, only the combination reduced FJB+ degenerating neurons at 72 hours. In conclusion, a combination of melatonin and calpeptin exerted synergistic protection against post-reperfusion injury in a rat MCAO stroke model.

Abstract T MP111: Gpr124 Regulates Post-Stroke Cerebrovascular Integrity

Stroke ◽  
2014 ◽  
Vol 45 (suppl_1) ◽  
Author(s):  
Michael Mancuso ◽  
Junlei Chang ◽  
Carolina Maier-Albers ◽  
Cynthia Kosinski ◽  
Xibin Liang ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Blood Brain Barrier ◽  
Cerebral Artery ◽  
Hemorrhagic Transformation ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Post Stroke ◽  
Adult Mice ◽  
Cerebral Artery Occlusion

Introduction: GPR124/TEM5 is an orphan G-protein coupled receptor (GPCR) with a large extracellular domain. We and others have previously demonstrated that GPR124 exerts CNS-specific angiogenesis regulation with knockout mice exhibiting embryonic lethality from hemorrhagic glomeruloid vascular malformations in forebrain and neural tube (c.f. Kuhnert et al., Science , Nov 12;330(6006):985-9 . (2010)). Hypothesis: GPR124 regulates adult angiogenesis and blood-brain barrier (BBB) integrity during homeostasis or after stroke. Methods: To bypass GPR124 embryonic lethality, we generated GPR124 conditional knockout (cko) mice allowing temporally-regulated deletion. Tamoxifen treatment of GPR124 flox/- ; ROSA-CreER mice versus GPR124 flox/+; ROSA-CreER littermate controls allowed GPR124 cko versus heterozygosity, respectively, in adult mice. GPR124 deletion was followed by analyses of microvascular structure and patterning and blood-brain barrier (BBB) integrity). GPR124 cko mice versus controls were also subjected to 60 minute middle cerebral artery occlusion (MCAO) and effects on stroke volume, survival and microvascular structure assessed. Results: GPR124 deletion in neonatal or adult mice was well-tolerated without impairment of postnatal vascular patterning, BBB maturation or BBB integrity. However, GPR124 cko mice subjected to the middle cerebral artery occlusion (MCAO) stroke model exhibited impaired survival and a profound microvascular hemorrhagic transformation that was confined to the infarct region relative to wild-type controls. GPR124 cko stroke vasculature also exhibited numerous cellular and architectural defects relative to controls that will be discussed. Conclusions: GPR124 deletion is well tolerated in adult mice but results in marked hemorrhagic transformation in the MCAO stroke model. GPR124 represents a novel receptor whose function is essential for cerebrovascular integrity in the post-stroke setting, with attendant therapeutic implications.

Water-soluble fullerene derivatives (C60(OH)18-22) decrease infarct volume and edema after middle cerebral artery occlusion in rat

2016 ◽  
Vol 2 (1) ◽  
pp. 80-89
Author(s):  
Masiha Aryafar ◽  
Mohammad Taghi Mohammadi ◽  
Zeinab Sadat Sobhani ◽  
Mahsa Sarami Foroshani ◽  
Javad Rasouli Vani
Keyword(s):  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Infarct Volume ◽  
Artery Occlusion ◽  
Water Soluble ◽  
Protective Effects ◽  
Fullerene Derivatives ◽  
Reperfusion Period ◽  
Cerebral Artery Occlusion

Overproduction of oxygen free radicals (ROS) and oxidative stress during reperfusion period plays a critical role in pathophysiology of cerebral ischemia-reperfusion injury. Since the antioxidant properties of fullerene nanoparticles has been extensively demonstrated, we assessed the possible protective effects of water-soluble fullerene derivatives (C60(OH)18-22) on infarct volume and edema after middle cerebral artery occlusion in rat.Twenty four rats were randomly divided into three groups (each group; n=8): sham, control ischemia (IR) and ischemic treatment groups. Cerebral ischemia was induced by 90 min middle cerebral artery occlusion (MCAO) followed by 24 hours reperfusion. Fullerene nanoparticles were given orally at dose of 1mg/kg immediately after termination of MCAO and the beginning of reperfusion period. The brains were processed for histopathological assessment and quantitation of the infarct volume (TTC staining method). Finally, the brain hemispheres were weighed as an index of brain edema.There was no infarction and edema in right hemispheres of sham rats. MCAO induced brain infarction both in cortex (261±23mm3) and subcortex (138±23mm3) and also right hemispheres weights (23%) of control ischemic rats. Administration of fullerene reduced the infarction both in cortex (49±26mm3) and subcortex (43±21mm3) and also the value of right hemisphere weight (20%) of ischemic treatment rats. Additionally in histopathological assessment, the perivascular areas decreased in ischemic treatment rats.The findings of present study are revealing the protective effects of water-soluble fullerene derivatives against reperfusion-induced injury such as infarction and edema after middle cerebral artery occlusion in rat.

scholarly journals Middle Cerebral Artery Occlusion Model in Rodents: Methods and Potential Pitfalls

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Fudong Liu ◽  
Louise D. McCullough
Keyword(s):  
Ischemic Stroke ◽  
Middle Cerebral Artery ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Occlusion Model ◽  
Cerebral Artery Occlusion ◽  
Paradoxical Results ◽  
Age And Sex

A variety of animal models have been developed for modeling ischemic stroke. The middle cerebral artery occlusion (MCAO) model has been utilized extensively, especially in rodents. While the MCAO model provides stroke researchers with an excellent platform to investigate the disease, controversial or even paradoxical results are occasionally seen in the literature utilizing this model. Various factors exert important effects on the outcome in this stroke model, including the age and sex of the animal examined. This paper discusses emerging information on the effects of age and sex on ischemic outcomes after MCAO, with an emphasis on mouse models of stroke.

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