scholarly journals Improved Adventitious Shoot Organogenesis and Callus Production from Leaf Explants of Petunia hybrida L. by Benzyladenine (BA) and Naphthalene Acetic Acid (NAA)

2019 ◽  
Vol 8 (4) ◽  
pp. 73-85
Author(s):  
F. Nazari ◽  
Keyword(s):  
Acetic Acid ◽  
Petunia Hybrida ◽  
Shoot Organogenesis ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Naphthalene Acetic Acid ◽  
Callus Production ◽  
Adventitious Shoot Organogenesis

scholarly journals Adventitious shoot organogenesis from leaf explants of Portulaca pilosa L.

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shuangyan Chen ◽  
Yuping Xiong ◽  
Xincheng Yu ◽  
Jinhui Pang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Shoot Organogenesis ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Adventitious Shoot Organogenesis

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

Adventitious shoot organogenesis and encapsulation technology in hop (Humulus lupulus L.)

2020 ◽  
Vol 270 ◽  
pp. 109416 ◽  
Author(s):  
Claudia Maria Liberatore ◽  
Margherita Rodolfi ◽  
Deborah Beghè ◽  
Andrea Fabbri ◽  
Tommaso Ganino ◽  
...  
Keyword(s):  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Humulus Lupulus ◽  
Humulus Lupulus L ◽  
Adventitious Shoot Organogenesis

scholarly journals Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.)

2014 ◽  
Vol 32 (2) ◽  
pp. 170-179 ◽  
Author(s):  
Hernando Criollo ◽  
Margarita Perea ◽  
Mariano Toribio ◽  
Johanna Muñoz
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Plant Propagation ◽  
Cotyledonary Leaf

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 mM of NAA were added to the medium with sucrose (50.0 and 263.1 mM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 mM of NAA ; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 mM of NAA + 263.1 mM of sucrose (32%).

scholarly journals Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Tetraploid Watermelon

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 211-213 ◽  
Author(s):  
Michael E. Compton ◽  
D.J. Gray
Keyword(s):  
Shoot Organogenesis ◽  
Citrullus Lanatus ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Mature Seed ◽  
Regeneration Medium ◽  
Breeding Lines ◽  
Cotyledon Explants ◽  
Explant Source ◽  
Adventitious Shoot Organogenesis

Cotyledon explants of four watermelon [Citrullus lanatus (Thunb.) Mataum. & Nakai] breeding lines (F92U8, SP90-1, SP90-2, and SP90-4) were prepared from mature seed or from 2-, 4-, 6-, 8-, or 10-day-old seedlings. Explants were incubated on shoot regeneration medium for 8 weeks followed by 4 weeks on shoot elongation medium. The four genotypes differed in their ability to produce shoots at each explant age. The highest frequency with which F92U8 (66%) and SP90-2 (60%) explants produced shoots was for 2-day-old seedlings. Fewer explants formed shoots when established from mature seed or seedlings older than 2 days. In contrast, the percentage of SP90-4 explants that produced shoots was highest when cotyledons were obtained from 4-day-old seedlings (40%), but the response was less than the optimum for F92U8 and SP90-2. SP90-1 cotyledon explants exhibited the poorest response of the four breeding lines (<11% produced shoots), with little difference in response among the explant ages tested. The number of shoots per responding explant also depended on the age of the explant source. Explants from 2- to 4-day-old seedlings produced the most shoots. Fewer shoots formed on cotyledons from mature seed or seedlings older than 4 days.

scholarly journals Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant

2011 ◽  
Vol 77 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Arzu Ucar Turker ◽  
Esra Canserver Multu
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Skin Diseases ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Solanum Dulcamara ◽  
Petiole Explants ◽  
Indole 3 Acetic Acid

<em>Solanum dulcamara</em> L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.

Sign in / Sign up

Export Citation Format

Share Document