scholarly journals Effects of Aloe Vera and Chitosan Nanoparticle Thin-Film Membranes on Wound Healing in Full Thickness Infected Wounds with Methicillin Resistant Staphylococcus Aureus

2018 ◽  
Vol 6 (1 JAN) ◽  
pp. 8-15 ◽  
Author(s):  
Reza Ranjbar ◽  
◽  
Alireza Yousefi
Keyword(s):  
Thin Film ◽  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aloe Vera ◽  
Full Thickness ◽  
Methicillin Resistant ◽  
Chitosan Nanoparticle ◽  
Infected Wounds

Abstract 10200: Translational Research of Novel Angiogenic Peptide for Wound Healing Drug; From Discovery to Clinical Trial

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Hironori Nakagami ◽  
Ryuichi Morishita
Keyword(s):  
Clinical Trial ◽  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Drug Development ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Skin Ulcer ◽  
Secondary Outcome ◽  
Double Blind Study ◽  
Full Thickness ◽  
Methicillin Resistant

We developed a novel cationic peptide, AG30/5C, with angiogenic properties. Since it has both angiogenic and antimicrobial actions, it might be a candidate peptide for wound healing drug. We conducted a physician-initiated clinical trial of AG30/5C for severe skin ulcer in patients as an open label study. The primary outcome is safety because it is first in human trial on early stage. AG30/5C was administered with patients with skin ulcer twice per day for two weeks, and ulcer size and Methicillin-Resistant Staphylococcus Aureus amount was quantified as a secondary outcome. Two patients was registered until now and the skin ulcer of first patient was completely cured after AG30/5C treatment. The Methicillin-Resistant Staphylococcus Aureus amount of both patients was decreased during the AG30/5C treatment. This result suggests the potential of AG30/5C as a wound healing drug, however, improvement of stability and cost efficiency are required toward drug development. Thus, we addressed to the metabolites of AG30/5C which can provide a further optimized compound, SR-0379. SR-0379 also enhanced proliferation, tube formation, migration and contraction on human dermal fibroblast cells via the PI3K-Akt-mTOR pathway through integrin-mediated interactions. We evaluated the effect of SR-0379 on two different would healing model in rats, the full-thickness defects in diabetic condition and an acutely infected wound with the full-thickness defects and inoculation with S aureus. Treatment of SR-0379 significantly accelerated the wound healing compared to that of FGF2. The beneficial effects of SR-0379 on wound healing would be explained by enhanced angiogenesis, granulation tissue formation, proliferation and antimicrobial activity. These results clearly demonstrated that SR-0379 may have a potential for drug development in wound repairing, in especially critical colonization condition. Based on these results, we now conducted the physician-initiated clinical trial for severe skin ulcer in patients as a double blind study.

scholarly journals Potential antimicrobial properties of the Ulva lactuca extract against methicillin-resistant Staphylococcus aureus-infected wounds: A review

2021 ◽  
pp. 1116-1123
Author(s):  
Nadya Fianny Ardita ◽  
Lenny Mithasari ◽  
Daris Untoro ◽  
Siti Isrina Oktavia Salasia
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Wound Infection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Healing Process ◽  
Ulva Lactuca ◽  
Wound Healing Process ◽  
Methicillin Resistant ◽  
Infected Wounds ◽  
Anti Inflammatory

Methicillin-resistant Staphylococcus aureus (MRSA), currently a major problem in hospitals worldwide, is one of the most common causes of nosocomial disease through surgical wound infection. MRSA-infected wounds have very low recovery rates and have become more problematic as some antibiotics are not effective against MRSA. Several antimicrobial and anti-inflammatory agents of green algae (Ulva lactuca) in the form of alkaloids, triterpenoids, steroids, saponins, and flavonoids have the potential to accelerate the wound healing process following MRSA wound infection. Various active compounds contained in the U. lactuca extract are thought to have multiple antibacterial and anti-inflammatory properties that can overcome the MRSA antimicrobial resistance and accelerate tissue growth in the wound healing process. This review aims to describe the potential of Ulva lactuca extract against MRSA-infected wound healing.

scholarly journals Thin Film Biosensor for Rapid Detection of mecA from Methicillin-resistant Staphylococcus aureus

2000 ◽  
Vol 46 (9) ◽  
pp. 1501-1504 ◽  
Author(s):  
Robert Jenison ◽  
Ayla Haeberli ◽  
Shao Yang ◽  
Barry Polisky ◽  
Rachel Ostroff
Keyword(s):  
Thin Film ◽  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

Curcumin Nanoparticles Improved Diabetic Wounds Infected With Methicillin-Resistant Staphylococcus aureus Sensitized With HAMLET

2020 ◽  
pp. 153473462093307
Author(s):  
Saeed Taghavifar ◽  
Fatemeh Afroughi ◽  
Maryam Saadati Keyvan
Keyword(s):  
Staphylococcus Aureus ◽  
Topical Application ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Male Rats ◽  
Methicillin Resistant ◽  
Sterile Saline ◽  
Curcumin Nanoparticles ◽  
Significant Difference ◽  
Infected Wounds ◽  
Diabetic Wounds

Accurately orchestrated course of events normally observed in healing are not followed in diabetic wounds, and bacterial colonization/infection further messes up the process. Novel therapeutic options for treatment of infections caused by multidrug-resistant Staphylococcus aureus are urgently needed. HAMLET (human α-lactalbumin made lethal to tumor cells) has been reported to be able to sensitize bacterial pathogens to traditional antimicrobial agents. The aim was to assess the wound healing activity of curcumin nanoparticles in diabetic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) sensitized with HAMLET. Fifty male rats were randomized into 5 groups of 10 animals each. In CONTROL group, 0.1-mL sterile saline 0.9% solution was added to the wounds with no infection. In MRSA group, the wounds were infected with MRSA and only treated with 0.1-mL sterile saline 0.9% solution. In MRSA/HAMLET group, infected wounds were treated with HAMLET (100 µg). In MRSA/CNP group, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL curcumin nanoparticles. In MRSA/CNP/HAMLET group, animals with infected wounds were treated with topical application of 0.1 mL solution of curcumin nanoparticles (1 mg/mL) and HAMLET (100 µg). All test formulations were applied for 10 days, twice a day, starting from first treatment. Microbiological examination; planimetric, biochemical, histological, and quantitative morphometric studies; immunohistochemical staining for angiogenesis; determination of hydroxyproline levels; and reverse transcription polymerase chain reaction for caspase 3, Bcl-2, and p53 showed that there was significant difference between animals in MRSA/CNP/HAMLET group compared with other groups ( P < .05). Curcumin nanoparticles improved diabetic wounds infected with MRSA sensitized with HAMLET and had the potential to offer more attention to this safer agent for topical use in infected diabetic wounds.

Nanoemulsion is an effective antimicrobial for methicillin-resistant Staphylococcus aureus in infected wounds

Nanomedicine ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. 1177-1185 ◽  
Author(s):  
Zhengyi Cao ◽  
Theodore Spilker ◽  
Yongyi Fan ◽  
Linda M Kalikin ◽  
Susan Ciotti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Infected Wounds

scholarly journals Nano Spray TRISWHEAT (Teripang Super Wound Healing Agent) Penyembuh Luka Diabetes Mellitus yang Terinfeksi Bakteri MRSA (Methicillin Resistant Staphylococcus aureus) dengan Ekstrak Teripang

2018 ◽  
Vol 36 (1) ◽  
pp. 40
Author(s):  
Nada Hanifah ◽  
Yusuf Farid Achmad ◽  
Mellya Permatasari ◽  
Marista Kurniati ◽  
Ditya Tiwi Syafira ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Sea Cucumber ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Healing Process ◽  
Wound Healing Process ◽  
Methicillin Resistant ◽  
Wound Size

Sea Cucumber (Stichopus sp) belongs to Holothuroidea family. Sea Cucumber has lot of uses in the medicine sector, such as: for wound healing, anti-biotic, anti-bacteria, anti-tumor, and anti-coagulation. Diabetes mellitus is a disease characterized by high blood sugar levels with metabolic disorders. This disease can cause wounds called gangrene This research has purpose to prove the influence of giving nano spray of sea cucumber extract within 14 days. In the in vitro examination found 40% concentration of sea cucumber extract which is effective to resist the growth of Methicillin Resistant Staphylococcus aureus bacteria and examined to wistar mouse which are already diabetic inducted with Streptozotocin (STZ) and hurt with punch biopsy with the diameter of 0,8 cm on its back and the wound is infected with bacteria which is intradermal injected. Divided into 3 groups : (K-) no medical treatment, nano spray of sea cucumber extract (T), Nano spray penicillin (K+) to wistar mouse towards the speed of diabetic mellitus wound healing which is infected by Methicillin Resistant Staphylococcus aureus bacteria. Parameter used to measure the rate of wound healing is wound size and hitopathology examination.After it’s regularly given the medicine, the result of nano spray sea cucumber extract 40 % concetration category treatment on wound healing process the diameter of wound on the 3rd day 0.8 cm , on the 7th day 0.4 cm and on the 14th day  wound fully recover according to macros (wound size) and histopathology examination shows faster and better healing compared to treatment category of nano spray penicillin and control marked by score cell and lymphocyte in a small quantity.

Sign in / Sign up

Export Citation Format

Share Document