scholarly journals Comparison Of Total Serum Sulfhydryl and Glutathione S Transferase Activities in Patients With Oral Cavity Malignancies and Healthy Control Individuals

2020 ◽  
Vol Volume: 2 Issue: 2 (Volume: 2 Issue: 2) ◽  
pp. 72-75
Author(s):  
Şeyda Belli- Şeyda Belli, Halit Demir, Bilge Özdemir, Canan demir
Keyword(s):  
Oral Cavity ◽  
Total Serum ◽  
Glutathione S Transferase ◽  
Healthy Control

Analysis of glutathione S-transferase genes polymorphisms and the risk of schizophrenia in a sample of Iranian population

2011 ◽  
Vol 7 (2-4) ◽  
pp. 199-203 ◽  
Author(s):  
Farah Lotfi Kashani ◽  
Dor Mohammad Kordi-Tamandani ◽  
Roya Sahranavard ◽  
Mohammad Hashemi ◽  
Farzaneh Kordi-Tamandani ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Case Control Study ◽  
Gene Interaction ◽  
Case Control ◽  
Glutathione S Transferase ◽  
Chain Reaction ◽  
Healthy Control ◽  
Glutathione S Transferases ◽  
Polymerase Chain ◽  
Control Study

Glutathione S-transferases (GSTs) are major intracellular antioxidants, which, impaired in their function, are involved in the progress of schizophrenia (SCZ). The aim of this case-control study was to investigate the association between the polymorphism of glutathione S-transferases M1 (GSTM1), T1 (GSTT1), the glutathione S-transferase P1 gene (GSTP1) and SCZ. We isolated genomic DNA from peripheral blood of 93 individuals with SCZ and 99 healthy control subjects' genotypes analyzing them for GSTM1, GSTT1 and GSTP1 using polymerase chain reaction. The analysis of the gene–gene interaction between GSTs indicated that the magnitude of the association was greater for the combined AG/GSTT1 & GSTM1 genotypes (OR = 2.51; 95% CI: 1.13–5.63, P = 0.02). The AG and combined AG + GG genotypes of GSTP1 increased the risk of SCZ (OR = 1.83; 95% CI: 0.94–3.75 and OR = 1.71; 95% CI: 0.92–3.19, respectively). The genotypes of GSTT/NULL, NULL/GSTM and NULL/NULL increased the risk of SCZ (OR = 2.05; 95% CI: 0.9–4.74; OR = 2.0; 95% CI: 1.68–2.31; and OR = 1.8; 95% CI: 0.57–2.46, respectively). The present study supports previous data that suggest that impairment in the function of GSTs genes may increase the risk of SCZ.

scholarly journals Some Biochemical parameters Of People With Atopic Eczema And There Comparison With Normal People

2019 ◽  
Vol 23 (10) ◽  
pp. 1
Author(s):  
Azeez Khalid Hameed ◽  
Buraq Hikmat Alwan
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Atopic Eczema ◽  
Total Protein ◽  
Serum Bilirubin ◽  
Blood Analysis ◽  
Total Serum ◽  
Control Group ◽  
Healthy Control ◽  
And Control

The present study aimed to identify the biochemical changes in sera of patients with Atopic Eczema, which included some blood analysis included serum urea, uric acid, creatinine, cholesterol, blood glucose, albumin, total protein, calcium, GPT, ALP, total serum bilirubin. Samples were collected from 19 August to 19 December in Salahelddin Hospital in tikrit city, the study included 94 sample, 65 from patients with Atopic Eczema, and 29 healthy people as a control group, the results were compared between the Atopic Eczema patients group and the healthy control group, and there was elevation in blood urea, uric acid, ceatinine, total protein, GPT, ALP, TSB,( 34.9+22.10), (5.49 + 4.38), (0.788+ 0.669), (7.34 +6.41), (13.38 +6.96), (13.63+ 8.52), (0.831+0.190),  respectively. And there was no elevation in,  total albumin, cholesterol, calcium, blood glucose, (4.595+4.321), (161.5+ 148.1), (8.96 + 9.13), (109.3+ 105.3), respectively when compared between patients group and control group.   http://dx.doi.org/10.25130/tjps.23.2018.161

scholarly journals Immunoprotective Effect of Seabuckthorn (Hippophae rhamnoides) and Glucomannan on T-2 Toxin-Induced Immunodepression in Poultry

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
T. Ramasamy ◽  
C. Varshneya ◽  
V. C. Katoch
Keyword(s):  
Treated Group ◽  
Phagocytic Index ◽  
Total Serum ◽  
Control Group ◽  
Humoral Immune ◽  
Healthy Control ◽  
Group A ◽  
Humoral Immune Reaction ◽  
Ppm Level ◽  
Immunoglobulin Levels

The present investigation was undertaken to study the immunoprotective effect of seabuckthorn berries and glucomannan against T-2 toxin-induced immunodepression in 15-day-old chicks. T-2 toxin was produced in the laboratory by growingFusarium sporotrichioidesMTCC 2081 on wheat. T-2 toxin was fed to birds at 1 ppm level of the diet. The powdered seabuckthorn berries were added at 400 and 800 ppm levels, and glucomannan added at 1 g/kg of feed. All the treatments were continued up to 28 days. The immunoprotective effects of seabuckthorn and glucomannan were assessed by evaluating humoral immune reaction against NCD vaccine (haemagglutination test and immunoglobulin estimation), serum immunoglobulin levels, phagocytic index, and DTH reaction against DNFB between day 25 and day 28 of experiment. There was significant (P<.05) decrease in non-specific immunity in T-2 toxin-treated group as evidenced by a reduction in phagocytic index, DTH reaction, HI titer, and total serum Ig compared to the healthy control group. A significant increase (P<.05) in HI titer and total serum Ig was seen in seabuckthorn and glucomannan fed group. A significant (P<.05) increase in DTH reaction and non-specific immune response was seen in seabuckthorn and glucomannan fed birds. The present investigation revealed that the seabuckthorn alone protected the immunosuppressant action of T-2 toxin, but seabuckthorn and glucomannan in combination provided an additive protection against T-2 toxicity.

scholarly journals Glutathione-S-Transferase M1 and T1 Gene Polymorphisms and Susceptibility to the Progression of Liver Fibrosis in Hcv-Infected Patients in Taiwan

2014 ◽  
Vol 33 (3) ◽  
pp. 259-264 ◽  
Author(s):  
Fang-Chun Sun ◽  
Ya-Chung Jeng ◽  
Meng-Shiou Lee ◽  
Chen-Fan Wen ◽  
Tsung-Ming Chen ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Odds Ratio ◽  
Gene Polymorphisms ◽  
Healthy Controls ◽  
Hepatitis C Virus Infection ◽  
Glutathione S Transferase ◽  
Significant Relation ◽  
Gstt1 Gene ◽  
Healthy Control ◽  
T1 Gene

Abstract Background: This study was conducted to evaluate the influence of glutathione-S-transferase (GST) M1 and T1 polymorphisms in 184 patients with different stages of liver fibrosis and hepatitis C virus infection and 173 healthy control subjects. Methods: DNA samples were extracted from whole blood, and the polymorphisms of GSTM1 and GSTT1 were determined with PCR using fluorescence-labeled Taq Man probes. Associations between specific genotypes and progression of liver fibrosis were examined by use of the logistic regression analysis to calculate the odds ratio (OR) and 95% confidence intervals (CI). Results: Results show that no differences were found between the frequencies of GSTM1 (49.8% versus 50.2%) and GSTT1 (52.2% versus 47.8%) null genotypes in HCV-infected pa tients and healthy controls, respectively. In addition, there was also no significant relation between the frequency of GSTM1 or GSTT1 gene polymorphisms and fibrosis stage as classified by the METAVIR group. Conclusions: The combined GSTM1 and GSTT1 null genotypes showed an association between GSTM1 [-]/GSTT1 [- ] and progression of liver fibrosis.

scholarly journals Chewing time and chewing strokes in different dentofacial deformities

2012 ◽  
Vol 24 (2) ◽  
pp. 130-133 ◽  
Author(s):  
Melissa Nara de Carvalho Picinato-Pirola ◽  
Francisco Veríssimo de Mello-Filho ◽  
Luciana Vitaliano Voi Trawitzki
Keyword(s):  
Oral Cavity ◽  
Class Ii ◽  
Class Iii ◽  
Dentofacial Deformities ◽  
Dentofacial Deformity ◽  
Healthy Control ◽  
Opening And Closing

PURPOSE: To verify whether the number of chewing strokes and the chewing time are influenced by dentofacial deformities in habitual free mastication. METHODS: Participants were 15 patients with diagnosis of class II dentofacial deformity (GII), 15 with class III (GIII), and 15 healthy control individuals with no deformity (CG). Free habitual mastication of a cornstarch cookie was analyzed, considering the number of chewing strokes and the time needed to complete two mastications. Strokes were counted by considering the opening and closing movements of the mandible. The time needed to consume each bite was determined using a digital chronometer, started after the placement of the food in the oral cavity and stopped when each portion was swallowed. RESULTS: There were no differences between groups regarding both the number of strokes and the chewing time. However, with regards to the number of strokes, CG and GII presented a significant concordance between the first and the second chewing situation, which was not observed in GIII. The analysis of time showed significant concordance between the first and second chewing situation in CG, reasonable concordance in GII, and discordance in GIII. CONCLUSION: Dentofacial deformities do not influence the number of chewing strokes or the chewing time. However, class III individuals do not show uniformity regarding these aspects.

scholarly journals GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD

2014 ◽  
Vol 64 (1) ◽  
pp. 117-129 ◽  
Author(s):  
Irena Žuntar ◽  
Roberta Petlevski ◽  
Slavica Dodig ◽  
Sanja Popović-Grle
Keyword(s):  
Genetic Polymorphisms ◽  
Odds Ratio ◽  
Statistical Difference ◽  
Smoking History ◽  
Total Serum ◽  
Wild Type ◽  
Glutathione S Transferase ◽  
Mutant Genotype ◽  
Larger Sample ◽  
Gst Activity

Abstract The aim of this study was to test the hypothesis that glutathione- S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non- -smokers, ex-smokers, and smokers) with stable COPD (n = 30) and sex/age matched controls (n = 60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed a statistical difference (p < 0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p = 0.002). In this study, the GSTP1 mutant genotype of exon 5 (GG), as well as GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT), were suggested to be genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and joint GSTM1/GSTT1 null genotypes were not disease associated. Serum GST was not associated with GST genotypes and COPD or smoking history in our study subjects. Conclusions drawn from the study should be further supported and clarified by studies with larger sample sizes.

scholarly journals Correlation between malondialdehyde and metanephrine in patients with acute lymphoblastic leukemia

2014 ◽  
Vol 11 (3) ◽  
pp. 1217-1225
Author(s):  
Baghdad Science Journal
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Serum Level ◽  
Blood Cell ◽  
Biochemical Parameters ◽  
Lymphoblastic Leukemia ◽  
Serum Glucose ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Cell Count ◽  
Healthy Control

Acute lymphoblastic leukemia (ALL) is one of the most common diseases , so in this study the serum level of malondialdehyde and its relationship with metanephrine was investigated in acute lymphoblastic leukemia patients over one month of treatment. Some biochemical parameters (serum glucose , total serum protein , malondialdehyde ,vitamin C, and metanephrine) changed as well as white blood cell count and blood hemoglobinlevelswere analyzed in sixty patients diagnosed with acute lymphoblastic leukemia over one month of treatment compared to healthy control group.Statistically significant increases (p

scholarly journals Polymorphisms of the GSTT1 and GSTM1 genes in women of central Serbia: Absence of association with uterine myoma

2013 ◽  
Vol 65 (2) ◽  
pp. 415-420 ◽  
Author(s):  
S. Arsenijevic ◽  
Biljana Ljujic ◽  
Ivana Stosic ◽  
D. Grujicic ◽  
D. Marinkovic ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Significant Risk ◽  
Uterine Myoma ◽  
Glutathione S Transferase ◽  
Cellular Protection ◽  
Healthy Control ◽  
Gst Polymorphism

Since glutathione S-transferase (GST) enzymes are involved in cellular protection, we aimed to determine the distribution of GSTT1 and GSTM1 null genotypes in women in central Serbia in order to assess the risk of development of uterine myoma. The study consisted of 34 clinically diagnosed uterine myoma patients and 35 healthy control women. Analyses of GST polymorphism were carried out by multiplex PCR. Our results showed no significant differences in the GSTT1 and GSTM1 null genotypes between the patients and controls. Using the GSTT1 positive/GSTM1 positive combination as reference, there was no statistically significant risk of uterine myoma with the combination of GSTT1 null and GSTM1 null genotypes. We conclude that polymorphism of both GSTT1 and GSTM1 genes, alone or in combination, did not present the main risk for uterine myoma in women from central Serbia.

scholarly journals Inflammatory Cytokines, Proteins, and White Blood Cells in Burned Patients Affected with Second and Third Degree of Burn

2018 ◽  
Vol 26 (5) ◽  
pp. 103-119
Author(s):  
Dakhel Ghani Omran AL-Watify
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
The Body ◽  
Total Serum ◽  
Control Groups ◽  
Burned Patient ◽  
Male Group ◽  
Healthy Control ◽  
Patient Groups ◽  
Second Degree

Burns are associated with increasing metabolic activities of the body organs. High metabolism rates are initiated by activation of different inflammatory reactions and cellular mediators( cytokines). The present research was conducted to evaluate the inflammatory markers , proteins , and white blood cells in thermal burned patients . A total number of burned patients was 60 from both sexes ( 30 males and 30 females), then, the patients were classified according to severity of burn into four subgroups , the first burned male group involved 15 burned males affected with second degree of burn, and the second burned male group(15) was affected with third degree of burn . Similarly, the same classification was applied on burned females in this study. Thirty healthy subjects (15 males, 15 females) were selected as control groups. All ages of patients and controls were ranged between 2 5-35 years old.   It was well found that the levels of C- reactive protein ( CRP) were markedly increased ( p < 0 . 05 ) in all patient groups in a comparison with their normal counterparts . Results of interleukin – 6 ( IL-6 ) were significantly heightened ( p< 0. 05) in most burned patient groups except male group affected with second degree of burn ,since, they did not show a significant increase ( p>0.0 5 ). Concentrations of total serum protein , albumin , and globulin were tend to decrease significantly ( p < 0 . 0 5 ) in most burned groups when compared with those healthy control groups.   Regarding total white blood cells (WBCs) count, data obtained from the present study indicated a significant increase (p<0.05) of total WBCs in all burned patient groups as matching with control groups. Furthermore, differential WBCs explained the follow-  ing abnormalities: lymphocyte levels were significantly decreased (p<0.05) in all patient groups. Inversely, values of monocytes indicated an insignificant elevation (p>0.05) in most burned groups except burned male group affected with third degree of burn which showed a significant increase (p<0.05) in comparison with healthy groups. Statistical analysis of t-test indicated that granulocytes pointed out a significant elevation (p<0.05) Neutrophils level in all studied groups. Inversely, values of eosinophils were signifi- cantly reduced (p<0.05) in most burned groups compared to healthy control groups. Sig- nificant and insignificant decreases were found in the levels of basophils of all burned groups when compared to those of control group. 

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