scholarly journals Effect of Lawsonia inermis extract on the pathological changes of skin infection by Streptococcus pyogens in lab. Mice

2011 ◽  
Vol 10 (1) ◽  
pp. 45
Author(s):  
B. M. M. Al-Mehna And E. A. H. Kadhum
Keyword(s):  
Skin Infection ◽  
Recovery Period ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Diffusion Method ◽  
Histopathological Study ◽  
Alcoholic Extract ◽  
Lawsonia Inermis

In this study, the antibacterial effects of aqueous and alcoholic extracts of Lawsonia inermis leaves against Streptococcus pyogenes were investigated in vitro by using agar well diffusion method and in vivo using laboratory mice by treating it with prepared ointment from these extracts & compared the effects wih Gentamicin . Results showed that both extracts demonstrated antimicrobial activity against the tested organism but the efficiency of the extracts was significantly affected by the solvent used in the extraction as well as the concentration of extract. Alcoholic extracts had the highest antibacterial activity it exhibited an inhibition zone 18.2-28.2 mm in comparison with 26.2 mm for gentamicin .A prepared ointment from alcoholic extract in a concentration 5% was tested for treatment of the experimentally infected skin of mice by S. pyogenes using scratching . The recovery period was 11 days in compared with 10 days for gentamicin. Biopsy was taken from the infected area to detect the pathological effects occurred by tested bacteria ,also biopsy was again taken after the treatment with the prepared ointment to evaluate the anti-inflammatory ,antibacterial and wound healing activity of Lawsonia inermis. Histopathological study showed after 3days from skin infection with Streptococcus pyogens hypremia, swelling, congestion and pus formation also abscessation While in treated animals with henna extracts gross skin lesions disappeare after 11 days from infection and on day 10 showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells when compared with the controls which showed inflammatory cells, scanty collagen fibres and fibroblasts.

scholarly journals Antibacterial and Toxicity Evaluation of Eastern Medicine Formulation Eczegone for the Management of Eczema

Dose-Response ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 155932582095679
Author(s):  
Muhammad Amjad Chishti ◽  
Ejaz Mohi-Ud-Din ◽  
Shahbaz Ahmad Zakki ◽  
Muhammad Rahil Aslam ◽  
Sheraz Siddiqui ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Safety Evaluation ◽  
Diffusion Method ◽  
In Vitro Toxicity ◽  
Klebsiella Pneumonia ◽  
Lawsonia Inermis ◽  
Disk Diffusion Method ◽  
Skin Keratinocytes

The present study was conducted to evaluate the antibacterial activity, in vitro and in vivo cytotoxicity, cell viability and safety of Eastern Medicine coded medicinal formulation Eczegone comprising extracts of Azadirachta indica (Azin) , Fumaria indica (Fuin) , Sphaeranthus indicus (Spin) and Lawsonia inermis (Lain). This work also evaluated antibacterial activity of Eczegone formulation having above mentioned plants ethanolic extracts against different bacteria’s by disk diffusion method. In vitro toxicity of Eczegone formulation was investigated by using human skin keratinocytes HaCaT cell line, crystal violet stained cells, and methyl tetrazolium cytotoxicity (MTT) assay. In vivo acute oral and dermal cytotoxicity was determined by using Swiss albino mice and albino rabbits, respectively. The Eczegone formulation showed antibacterial activity against 3 gram negative bacteria including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris and a gram positive Staphylococcus aureus. We didn’t observe any toxic effect of Eczegone formulation on the skin keratinocytes. Furthermore, the Ezcegone formulation was non-irritant according to draize score (OECD TG404, 2002). After rigorous safety evaluation by in vitro and in vivo acute oral and dermal toxicity analysis, we concluded that Eczegone formualtion possessses antibacterial effects and is safe, non-toxic, non-irritant, and the drug would be subjected for further biochemical and clinical studies.

Anti-inflammatory properties of the carotenoids and polyphenols of pumpkin (Cucurbita moschata Duchesne)

2018 ◽  
Vol 7 (3) ◽  
pp. 100-106
Author(s):  
Bouamar Sarah ◽  
Mokhtar Meriem ◽  
Bouziane Nabil ◽  
Boukazzoula Kamel ◽  
Riazi Ali
Keyword(s):  
Inflammatory Cells ◽  
Histopathological Study ◽  
Cucurbita Moschata ◽  
Membrane Stabilization ◽  
In Vivo Test ◽  
Reactive Protein ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

The purpose of this study was to investigate the anti-inflammatory activities of carotenoids and polyphenols extracts of pumpkin (Cucurbita moschata Duchesne). The evaluation was conducted using in vitro (BSA: bovine serum albumin and HRBC: Human Red Blood Cell membrane stabilization) and in vivo (Carrageenan-Induced Inflammation) tests. Pumpkin extracts obtained with organic solvents exhibited a good denaturation inhibition with 66 and 53% in the presence of 1000 μg/mL of carotenoids and polyphenols, respectively. In the HRBC membrane stabilization test, carotenoids had a better protection effect than polyphenols (66.17% vs 53.88%). In the in vivo test, polyphenol and carotenoid extracts were able to suppress the carrageenan-induced increases in paw thickness; carotenoids exerted a higher inhibition than the standard diclofenac. Both natural compounds decreased also C-Reactive Protein (CRP) production and lymphocytes, granulocytes and mid-sized cells. The histopathological study showed an accumulation of infiltrated inflammatory cells after the injection of carrageenan, but this inflammatory response was reduced in the pumpkin-treated animals. Pumpkin carotenoids and polyphenols reduced the neutrophils in the inflamed tissues. These findings suggest that polyphenols and carotenoids of pumpkins repre-sent good candidates as anti-inflammatory drugs.

scholarly journals Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

2019 ◽  
Vol 21 (1) ◽  
pp. 77 ◽  
Author(s):  
Bo Song ◽  
Ruiteng Song ◽  
Min Cheng ◽  
Hairong Chu ◽  
Fang Yan ◽  
...  
Keyword(s):  
Psoriasis Vulgaris ◽  
Animal Experiments ◽  
Inflammatory Cells ◽  
Percutaneous Treatment ◽  
Skin Lesions ◽  
Inflammatory Factors ◽  
Psoriatic Skin ◽  
Medical Materials

An exopolysaccharides/calcipotriol (EPS/CPT) emulsion was prepared using bacterial EPS as emulsifier, sunflower oil as an oil phase and CPT as the loaded drug, and the effect of this emulsion on psoriasis vulgaris treatment was evaluated. An EPS composed of mannose (70.56%) and glucose (29.44%) was obtained from the marine mangrove bacteria Bacillus amyloliquefaciens ZWJ (Zhu Wenjing) strain. The EPS has significant emulsifying activity at the concentration of 1.5%. The prepared EPS/CPT emulsion has small and stable particle size, with a drug content of 0.00492%, and good spreading properties. The in vitro drug release results revealed that the emulsion showed a certain sustained release effect. In vitro and in vivo animal experiments show that the EPS/CPT emulsion can effectively treat psoriasis vulgaris by increasing the accumulation of CPT in psoriatic skin lesions and reducing the levels of inflammatory cells and inflammatory factors (TNF and IL6). Additionally, it has a certain effect on reducing the side effects associated with CPT. This study lays a foundation for the research of EPS in the topical application of medical materials and treatment of psoriasis.

Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

2020 ◽  
Vol 18 ◽  
Author(s):  
Zirui Zhang ◽  
Shangcong Han ◽  
Panpan Liu ◽  
Xu Yang ◽  
Jing Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Tissue Injury ◽  
Inflammatory Cells ◽  
Pcr Analysis ◽  
Protective Effects ◽  
Deep Tissue ◽  
Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

Traditional Herbal Medicines, Newer Herbs and Other Novel Approaches Integrated in Herbal Medicine for Atopic Dermatitis-A Narrative Review

2020 ◽  
Vol 15 (3) ◽  
pp. 194-208
Author(s):  
Pravin Kumar ◽  
Dinesh Kumar Sharma ◽  
Mahendra Singh Ashawat
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicines ◽  
Developed Countries ◽  
Skin Lesions ◽  
Scientific Data ◽  
Herbal Drugs ◽  
Biological Drugs ◽  
Alternative Treatment Option

Atopic Dermatitis (AD) is a prolonged reverting skin ailment with characteristically distributed skin lesions. In the previous decades, researchers had shown a marked interest in AD due to its increased prevalence in developed countries. Although different strategies including biological and immune modulators are available for the treatment of AD, each has certain limitations. The researchers had shown considerable interest in the management of AD with herbal medicines. The establishment of herbal drugs for AD might eliminate local as well as systemic adverse effects associated with long term use of corticosteroids and also higher cost of therapy with biological drugs. The present review discusses the traditional East Asian herbal medicines and scientific data related to newer herbal extracts or compositions for the treatment of AD. In vivo animal models and in vitro cell cultures, investigated with herbal medicines to establish a possible role in AD treatment, have also been discussed in the paper. The paper also highlights the role of certain new approaches, i.e. pharmacopuncture, a combination of allopathic and herbal medicines; and novel carriers (liposomes, cubosomes) for herbal drugs on atopic skin. In conclusion, herbal medicines can be a better and safe, complementary and alternative treatment option for AD.

scholarly journals Therapeutic effects of EGF-modified curcumin/chitosan nano-spray on wound healing

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Yue Li ◽  
QingQing Leng ◽  
XianLun Pang ◽  
Huan Shi ◽  
YanLin Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Chitosan Nanoparticles ◽  
Skin Lesions ◽  
In Vitro Assays ◽  
Therapeutic Effects ◽  
Skin Defects ◽  
Post Operation

Abstract Dermal injury, including trauma, surgical incisions, and burns, remain the most prevalent socio-economical health care issue in the clinic. Nanomedicine represents a reliable administration strategy that can promote the healing of skin lesions, but the lack of effective drug delivery methods can limit its effectiveness. In this study, we developed a novel nano-drug delivery system to treat skin defects through spraying. We prepared curcumin-loaded chitosan nanoparticles modified with epidermal growth factor (EGF) to develop an aqueous EGF-modified spray (EGF@CCN) for the treatment of dermal wounds. In vitro assays showed that the EGF@CCN displayed low cytotoxicity, and that curcumin was continuously and slowly released from the EGF@CCN. In vivo efficacy on wound healing was then evaluated using full-thickness dermal defect models in Wistar rats, showing that the EGF@CCN had significant advantages in promoting wound healing. On day 12 post-operation, skin defects in the rats of the EGF@CCN group were almost completely restored. These effects were related to the activity of curcumin and EGF on skin healing, and the high compatibility of the nano formulation. We therefore conclude that the prepared nano-scaled EGF@CCN spray represents a promising strategy for the treatment of dermal wounds.

scholarly journals Optimized Chitosan/Anion Polyelectrolyte Complex Based Inserts for Vaginal Delivery of Fluconazole: In Vitro/In Vivo Evaluation

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 227 ◽  
Author(s):  
Bayan Darwesh ◽  
Hibah Aldawsari ◽  
Shaimaa Badr-Eldin
Keyword(s):  
Vaginal Delivery ◽  
Polyelectrolyte Complex ◽  
Inflammatory Cells ◽  
Antifungal Drugs ◽  
Vaginal Candidiasis ◽  
Histological Evaluation ◽  
Anionic Polymer ◽  
In Vivo Evaluation

(1) Background: Fluconazole, used orally for vaginal candidiasis, has reported gastrointestinal side effects. Therefore, researchers directed towards the drug vaginal delivery. However, vaginal delivery is limited by poor retention and leakage. Thus, this work aimed at exploring chitosan/anion polyelectrolyte complex (PEC) for the formulation of fluconazole vaginal inserts with controlled release and appreciable mucoadhesion. (2) Methods: PECs were prepared and assessed for interactions. Fluconazole PEC based vaginal inserts were prepared by lyophilization using mannitol. 3151 factorial design was applied to investigate the effect of the anion type and Chitosan/anion ratio on the inserts mucoadhesion and release properties. The optimized insert [based on 5:5 chitosan: anionic polymer (sodium alginate)] release was modulated by the release retardant; Compritol® 888. The selected formulation was subjected to microbiological and histological evaluation. (3) Results: Fluconazole inserts showed satisfactory drug content, acceptable friability percentages and highest swelling indices at six hours. Statistical analysis showed significant effect of the studied factors on detachment force and release properties. Microbiological assays revealed significantly higher antifungal activity of inserts compared to fluconazole solution. Reduced inflammatory cells were confirmed by histological evaluation. (4) Conclusion: CH/Alg based vaginal insert could be a promising platform for vaginal delivery of antifungal drugs used for vaginal candidiasis treatment.

Equilibrium distribution of radioxenon in tissue: xenon-hemoglobin association curve

1961 ◽  
Vol 16 (6) ◽  
pp. 1065-1070 ◽  
Author(s):  
Hadley L. Conn
Keyword(s):  
Partition Coefficients ◽  
Equilibrium Distribution ◽  
Hemoglobin Concentration ◽  
Gas Diffusion ◽  
In Vivo Studies ◽  
Diffusion Method ◽  
Body Tissues ◽  
Gas Diffusion Method

In vitro and in vivo studies were made of the equilibrium distribution of radioxenon in various organs and tissues of the dog and the xenon uptake compared with a water standard. Tissue-blood partition coefficients were calculated. The radioxenon-hemoglobin association curve was determined for dog and human hemoglobin and methemoglobin. The uptake of radioxenon by blood, due in particular to xenon-hemoglobin affinity, was appreciably greater than uptake either by water or by most other body tissues. Fat and brain were notable exceptions. Consequently, tissue-blood partition coefficients were about eight for fat, one for brain, and significantly less than one for other tissues studied. Acceptable accuracy for blood flow determinations with a radioxenon inert gas diffusion method would seem to depend on the use of a partition coefficient correction in turn corrected at least for the existing hemoglobin concentration. The uptake of xenon by hemoglobin had the characteristics of a solubility or a quasi-solubility phenomenon. The problem of the nature of the interaction is apparently not resolved. Submitted on June 19, 1961

Cigarette smoking, emphysema, and damage to alpha 1-proteinase inhibitor

1994 ◽  
Vol 266 (6) ◽  
pp. L593-L611 ◽  
Author(s):  
M. D. Evans ◽  
W. A. Pryor
Keyword(s):  
Cigarette Smoke ◽  
Proteinase Inhibitor ◽  
Inflammatory Cells ◽  
Lavage Fluid ◽  
Lung Lavage ◽  
Tissue Destruction ◽  
Phagocytic Cells ◽  
Lung Fluid

The proteinase-antiproteinase theory for the pathogenesis of emphysema proposes that the connective tissue destruction associated with emphysema arises from excessive proteinase activity in the lower respiratory tract. For this reason, the relative activities of neutrophil elastase and alpha 1-proteinase inhibitor (alpha 1-PI) are considered important. Most emphysema is observed in smokers; therefore, alpha 1-PI has been studied as a target for smoke-induced damage. Damage to alpha 1-PI in lung fluid could occur by several mechanisms involving species delivered to the lung by cigarette smoke and/or stimulated inflammatory cells. Oxidative damage to alpha 1-PI has received particular attention, since both cigarette smoke and inflammatory cells are rich sources of oxidants. In this article we review almost two decades of research on mechanistic studies of damage to alpha 1-PI by cigarette smoke and phagocytic cells in vitro, studies emphasizing the importance of elastinolytic activity in the pathogenesis of emphysema in vivo and studies of human lung lavage fluid to detect defects in alpha 1-PI at the molecular and functional levels.

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