scholarly journals Histopathological study of Effects of continuous injection of dexamethasone on wound healing in rabbits

2011 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
F. Salim
Keyword(s):  
Wound Healing ◽  
Control Animal ◽  
Inflammatory Cell ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Study ◽  
Continuous Injection ◽  
Made In

In this study 16 rabbit was divided in to two groups equally the first group was daily injected by dexamethasone(1 mg/Kg) for 21 days,the second group was left without any treatment as a control. An excision was made in the skin of rabbits and then sutured with silk band 2.5cm.The skin spicemen were taken for histopathological sections for wound healing evaluation at postoperative days 3,7and 14. The scars of the dexamethasone-treated group were less formation. The epithelization ,collagenization and the inflammatory cell infiltration was less intense in the dexamethasone- treated group in compare with the control animal.

scholarly journals Topical Application of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) Accelerates Wound Healing in Diabetic Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Abdulmohsen H. Al-Elq ◽  
Mir Sadat-Ali ◽  
Mohamed Elsharawy ◽  
Ibrahim Al-Habdan ◽  
Fatin Othman Al-Aqeel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Collagen Deposition ◽  
Peptide Group ◽  
Diabetic Rabbits

Objective. Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.Materials and Methods. Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.Results. There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.Conclusion. Topical application of SHMSP improves wound healing in diabetic rabbits.

scholarly journals The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study

2021 ◽  
pp. 153473462110552
Author(s):  
Başak Büyük ◽  
Cemre Aydeğer ◽  
Yasemen Adalı ◽  
Hüseyin Avni Eroğlu
Keyword(s):  
Wound Healing ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Female Rats ◽  
Cell Infiltration ◽  
Fibroblast Proliferation ◽  
Accelerate Wound Healing ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound

Background: Wound healing has a vital importance for the organism and various agents are used to accelerate wound healing. Although the effect of boron on wound healing is known, its mechanisms are not completely clear yet. In this study, the effect of boron in the Ephrin /Eph pathway will be evaluated. Methods: Forty adult female rats were used in the study. A full-thickness excisional wound model was created in all groups divided as Control, Fito, Boron and Plu groups. After the applications performed twice a day and lasting 7 days, skin tissues obtained and evaluated histopathological (inflammatory cell infiltration, oedema, and fibroblast proliferation density) and immunohistochemical (TNF-α, EphrinA1, EphrinB1, EphrinB2 and EphB4). Results: Inflammatory cell infiltration score was found to be higher in the Fito group compared to Boron group (p = .018). Fibroblast proliferation density was higher in Plu group than Boron group (p = .012). While TNF-α was lower in boron group than Plu (p = .027) and Fito (p = .016) groups, EphrinA1 was higher in Boron group than Plu group (p = .005). EphrinB1 expression was higher in Boron group compared to Plu (p = .015) and Fito (p = .015) groups, and the same difference was also observed in EphrinB2 (p values .000). Similarly, EphB4 immunoreactivity was higher in the Boron group compared to Plu (p = .000) and Fito (p = .002). Conclusion: One of the mechanisms of action of boron in wound healing is to increase EphrinB1, EphrinB2 and EphB4. Low TNF-α and histopathological findings indicate that boron limits extensive wound healing.

The effect of OK-432 (Picibanil) injection on the histopathology of nasal turbinate

2015 ◽  
Vol 129 (12) ◽  
pp. 1208-1212
Author(s):  
S Sengul ◽  
İ Kaygusuz ◽  
M M Akin ◽  
Ş Yalcin ◽  
T Karlidag ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Cell Loss ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Saline Control ◽  
Histopathological Changes ◽  
Nasal Turbinate ◽  
Treatment Groups ◽  
Significant Difference

AbstractObjective:This study aimed to assess the histopathological effect of OK-432 (Picibanil) on rabbit nasal turbinates.Methods:A total of 21 rabbits were divided into 3 treatment groups and various parts of both nasal turbinates were injected with 0.5 ml OK-432, 0.2 ml OK-432 or 0.6 ml saline (control). Bilateral nasal turbinates were later excised and studied under light microscopy to assess any histopathological changes.Results:Animals in the 0.2 ml and 0.5 ml OK-432 groups exhibited mild ciliary loss, goblet cell loss and epithelial damage, and a marked increase in inflammatory cell infiltration, submucosal vascularisation and fibrosis. There was a significant difference in histopathological changes between the two OK-432 treated groups. In addition, each OK-432 treated group had significantly more inflammatory cell infiltration, increased submucosal vascularisation and fibrosis compared with controls.Conclusion:The marked fibrosis observed in OK-432-injected turbinates may be responsible for a reduction in turbinate size.

scholarly journals Thymosin β4-sulfoxide attenuates inflammatory cell infiltration and promotes cardiac wound healing

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Mark A. Evans ◽  
Nicola Smart ◽  
Karina N. Dubé ◽  
Sveva Bollini ◽  
James E. Clark ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Thymosin Β4 ◽  
Cardiac Wound Healing

scholarly journals Histopathological study of genital organs in a naturally infected mare with dourine

2019 ◽  
Vol 26 (01) ◽  
pp. 17-22
Author(s):  
Mungun-Ochir B ◽  
Nyamdolgor U ◽  
Baatarjargal P ◽  
Soyolmaa G ◽  
Altanchimeg A
Keyword(s):  
Peripheral Nerve ◽  
Myelin Sheath ◽  
Inflammatory Cell ◽  
Deep Layer ◽  
Nerve Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Study ◽  
Vaginal Mucosa ◽  
Study Objective

The present study aimed to investigate pathological lesions occurred in both central and peripheral nervous systems in mare infected naturally with dourine. Nine years old mare, which was positive by PCR, examination of reproductive organ swabs and immune chromatographic testing and showed visible neurological signs of dourine was selected in this study. Objective of this study was to examine the patho-morphological changes in all genital organs of a naturally infected mare with T. equiperdum. Sections were stained with Hematoxylin- Eosin or other special staining solutions, and then observed under light microscopy. Histopathology results: Slight infiltration of inflammatory cell into peripheral nerve was observed. There was fragmented nuclei like microorganisms within the nerve fiber in the Utero-ovary ligament. Vagina: While inflammatory cell infiltration was seen in the vaginal mucosa, micro abscesses were also seen in the superficial of mucosa. There wasmicroorganism like exocytosis structure in the vaginal mucosa. There was perivascular and peripheral neural inflammatory cell infiltration in the vaginal deep layer. Inflammatory cell infiltration and myelin sheath degeneration were observed in the peripheral nerve of vulva and inflammatory cell infiltration, myelin sheath degeneration and microorganism or fragmented nuclei were observed in the peripheral nerve of vulva. Inflammatory cells infiltrated into the deep layer of vulva. Cross section of vagina nerve. CD20 positive cells (lymphocyte B) and CD3 positive cells (lymphocyte T) in nerve fibers Нийлүүлгийн өвчтэй гүүний эмгэг морфологийн судалгаа Хураангуй: Бидний судалгаанд полимеразын гинжин урвал (ПГУ) болон иммунохромотографийн түргэнтестээр (ИХТ) эерэг дүн үзүүлж, нийлүүлгийн өвчний мэдрэлийн илэрхий шинж тэмдэг үзүүлж буй 4 настай хээр гүүнд эмгэг анатомийн задлан шинжилгээ хийж, тархи, захын мэдрэлийн судлууд, дотор эрхтнүүд, арьс, тунгалгийн зангилаанууд болон үржлийн эрхтнүүдээс дээж авч, 10 хувийн буфержүүлсэн формалинд бэхжүүлэн, MNS 5451:2005 стандартын дагуу дээжийг боловсруулж, парафинд цутган, зүсмэгийг гематоксилин-эозиноор будаж, микроскопын шинжилгээ хийв. Өвчилсөн гүүний нүүрний зүүн талын мэдрэл саажиж, зүүн чих унжсан, дээд уруулын булчин баруун тийш мурийсан, доод уруулын булчин бага зэрэг саажсан зэрэг шинж тэмдэг үзүүлсэн байлаа. Үлэмж бүтцийн шинжилгээнийдүнгээр гүүний зүүн талын дээд уруулын өргөгч булчин, доод уруулын буулгагч булчин хатанхайрсан, бусад эрхтнүүд болох элэг, уушги, зүрх, бөөр, дэлүү, үржлийн эрхтэнд эмгэг өөрчлөлт илэрсэнгүй. Бичил бүтцийн судалгааны дүнгээр бүхий л захын мэдрэлүүд, үржлийн зарим эрхтнүүдийн мэдрэлийг хамарсан мэдрэлийн ширхгийн эмгэгшил буюу нейропати илэрсэн болно. Энэхүү судалгааны дүн нь байгалийн нөхцөлд нийлүүлгийн өвчнөөр өвчилсөн гүүнд эмгэг гистологийн өөрчлөлтийг нарийвчлан судалсан анхны үр дүнгүүдийн нэг юм. Түлхүүр үг: Гистологи, лимфоцит, иммуногистохими

Fucoidan reduces inflammatory response in a rat model of hepatic ischemia–reperfusion injury

2015 ◽  
Vol 93 (11) ◽  
pp. 999-1005 ◽  
Author(s):  
Xiao-jing Li ◽  
Qi-fa Ye
Keyword(s):  
Signaling Pathway ◽  
Inflammatory Cell ◽  
Ischemia Reperfusion ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Sulfated Polysaccharides ◽  
Control Group ◽  
Mrna Levels ◽  
Inflammatory Signaling

Ischemia–reperfusion (I/R) injury after a liver transplant is a major cause of severe complications that lead to graft dysfunction. Fucoidan, a complex of sulfated polysaccharides derived from marine brown algae, demonstrated antiapoptotic as well as potential anti-inflammatory properties in previous studies. Fucoidan has also shown protective effects on I/R-injured kidney and heart. However, whether fucoidan can attenuate hepatic I/R injury has not been examined. To clarify the role of fucoidan in hepatic I/R injury, Sprague–Dawley rats were subjected to sham operation or ischemia followed by reperfusion with treatment of saline or fucoidan (50, 100, or 200 mg·(kg body mass)−1·d−1). The fucoidan-treated group showed decreased levels of alanine aminotransferase and aspartate aminotransferase compared with the control group. Myeloperoxidase and malondialdehyde activities and mRNA levels of CD11b in the fucoidan-treated group were significantly decreased. Hepatocellular swelling/necrosis, sinusoidal/vascular congestion, and inflammatory cell infiltration were also attenuated in the fucoidan group. The expression of TNF-α, IL-6, IL-1β, CXCL-10, VCAM-1, and ICAM-1 were markedly decreased in the samples from the fucoidan-treated group. Fucoidan largely prevented activation of the inflammatory signaling pathway, compared with the control group. In summary, fucoidan can protect the liver from I/R injury through suppressing activation of the inflammatory signaling pathway, as well as the expression of inflammatory mediators, and inflammatory cell infiltration.

A Case of Bullous Amyloidosis Associated with an Inflammatory Cell Infiltration and Prominent Pruritus

2008 ◽  
Vol 70 (3) ◽  
pp. 269-273
Author(s):  
Taisuke KAMIYAMA ◽  
Yoshihiro KAWAGUCHI ◽  
Masami SASAKI ◽  
Masamichi SATOU ◽  
Kumiko MIURA ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration

scholarly journals The Symmetric 3D Organization of Connective Tissue around Implant Abutment: A Key-Issue to Prevent Bone Resorption

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Connective Tissue ◽  
Inflammatory Cell ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Collagen Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Oral Epithelium ◽  
Implant Abutment

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.

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