scholarly journals Safety evaluation of the food enzyme β‐cyclodextrin glucanotransferase from Escherichia coli strain WCM105xpCM6420

EFSA Journal ◽  
2020 ◽  
Vol 18 (10) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Safety Evaluation ◽  
Coli Strain ◽  
Cyclodextrin Glucanotransferase

scholarly journals Safety evaluation of the food enzyme α‐cyclodextrin glucanotransferase from Escherichia coli strain WCM105xpCM703

EFSA Journal ◽  
2020 ◽  
Vol 18 (10) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Safety Evaluation ◽  
Coli Strain ◽  
Cyclodextrin Glucanotransferase

scholarly journals Safety evaluation of the food enzyme maltogenic amylase from genetically modified Escherichia coli (strain BLASC)

EFSA Journal ◽  
2019 ◽  
Vol 17 (7) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Modified ◽  
Safety Evaluation ◽  
Coli Strain ◽  
Maltogenic Amylase

scholarly journals Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Escherichia coli strain K‐12 W3110 (pWKLP)

EFSA Journal ◽  
2021 ◽  
Vol 19 (4) ◽  
Author(s):  
◽  
Claude Lambré ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Modified ◽  
Safety Evaluation ◽  
Coli Strain ◽  
K 12

scholarly journals Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Julia Ittensohn ◽  
Jacqueline Hemberger ◽  
Hannah Griffiths ◽  
Maren Keller ◽  
Simone Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein C ◽  
Interleukin 1 ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Reporter Construct ◽  
Regulation Of Expression ◽  
E Coli ◽  
Strain Cft073 ◽  
Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

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