Opinion of the Scientific Panel on genetically modified organisms [GMO] on a request from the Commission related to the safety of foods and food ingredients derived from insect-protected genetically modified maize MON 863 and MON 863 × MON 810, for which

EFSA Journal ◽  
2004 ◽  
Vol 2 (4) ◽  
pp. 50 ◽  
Author(s):  
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Food Ingredients ◽  
Mon 810

scholarly journals Maize Plants Infestation by Fusarium spp. and Deoxynivalenol in Genetically Modified Corn Hybrid and Traditional Maize Cultivars

2011 ◽  
Vol 60 (4) ◽  
pp. 317-321 ◽  
Author(s):  
MAREK SELWET
Keyword(s):  
European Corn Borer ◽  
Genetically Modified ◽  
Pathogenic Fungi ◽  
Genetically Modified Maize ◽  
Corn Borer ◽  
Maize Cultivars ◽  
Maize Plants ◽  
Maize Cob ◽  
Grain Contamination ◽  
Mon 810

The objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against European corn borer (Ostrinia nubilalis Hbn) as well as selected non-modified cultivars. The plant material comprised the following genetically modified maize cultivar: DKC 3421 YG (MON 810) and non-modified cultivars obtained from Smolice Plant Breeding Ltd., IHAR Group: Junak (FAO 210-220), Prosna (FAO 220), SMH (FAO 230), Baca (FAO 220). Prior to harvesting, the occurrence of maize cob fusariosis was determined in the 89 (BBCH) developmental ripening stage. Microbiological assessment was carried out on grains selected from cobs characterized by various pathological symptoms. In 2008, a total of 133 isolates was obtained from the examined samples of infected maize plants, of which 51 isolates were species-identified, while in 2009, the total of 123 isolates were determined, of which 63 were species-identified. In both experimental years, the majority of isolates contained fungi from the Fusarium genus. The performed analysis of mean levels of cob contamination by fusarioses revealed that DKC 3421 YG (MON 810) and SMH (FAO 230) cultivars showed the smallest levels of contamination as well as the lowest percent of cob contamination per plant, while Junak (FAO 210-220) and Baca (FAO 220) cultivars were characterized by the highest degree of contamination. The lowest deoxynivalenol concentrations were determined in years 2008 and 2009 in the case of the DKC 3421 YG (MON 810) cultivar, whereas Prosna (FAO 220) cultivar was characterized by the highest deoxynivalenol concentration.

scholarly journals Detection of Genetically Modified Organisms in Foods by Protein- and DNA-Based Techniques: Bridging the Methods

2002 ◽  
Vol 85 (3) ◽  
pp. 787-791 ◽  
Author(s):  
Gert van Duijn ◽  
Ria van Biert ◽  
Henriette Bleeker-Marcelis ◽  
Ineke van Boeijen ◽  
Abdi Jama Adan ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Matrix Effects ◽  
Polyclonal Antibodies ◽  
Genetically Modified ◽  
False Negative ◽  
Detection Methods ◽  
Vegetable Crops ◽  
Food Ingredients ◽  
Negative Results ◽  
Maize Varieties

Abstract According to European Commission (EC) Regulation 1139/98, foods and food ingredients that are to be delivered to the final consumer in which either protein or DNA resulting from genetic modification is present, shall be subject to additional specific labeling requirements. Since 1994, genetically altered tomatoes, squash, potatoes, canola, cotton, and soy have been on the market. Recently, insect-resistant and herbicide-tolerant maize varieties have been introduced. Soy and maize are 2 of the most important vegetable crops in the world. During the past 4 years, both protein- and DNA-based methods have been developed and applied for detection of transgenic soy and maize, and their derivatives. For protein-based detection, specific monoclonal and polyclonal antibodies have been developed; for immunochemical detection, Western blot analysis and enzyme-linked immunosorbent assays are the most prominent examples. For detection of genetically modified organisms (GMOs) at the level of DNA, polymerase chain reaction-based methods are mainly used. For these reactions, highly specific primer sets are needed. This study compares the principally different methods. Specificity of methods and the possible risks of false-positive or false-negative results are considered in relation to sampling, matrix effects, and food processing procedures. In addition, quantitative aspects of protein- and DNA-based GM detection methods are presented and discussed. This is especially relevant as EC regulation 49/2000, which defines a threshold for an unintentional comingling of 1%, came into force on April 10, 2000.

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