scholarly journals Effect of Bacillus bacteria on activity of pathogen-induced proteins in potato plants and increasing their resistance to Phytophthora infestans (Mont.) de Bary

2020 ◽  
Author(s):  
V. O. Tsvetkov ◽  
L. G. Yarullina ◽  
G. F. Burkhanova ◽  
A. V. Sorokan
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Transcriptional Activity ◽  
Defensive Compounds ◽  
Potato Plants ◽  
Bacterial Treatment ◽  
Inhibitor Genes ◽  
Late Blight Pathogen ◽  
Induced Proteins

We studied the effect of the Bacillus bacteria on the content and activity of defensive compounds in potato plants upon infection with late blight pathogen. Bacterial treatment had a stimulating effect on the concentration of H2O2 and transcriptional activity of hydrolase inhibitor genes.

scholarly journals Influence of the genus Bacillus bacteria on the content of H2O2 and the activity of hydrolases and their inhibitors in potato plants during Phytophthora infestans Mont. de Bary infection

2020 ◽  
Vol 23 ◽  
pp. 02010
Author(s):  
Lyubov G. Yarullina ◽  
Antonina V. Sorokan ◽  
Vyacheslav O. Tsvetkov ◽  
Guzel F. Burkhanova ◽  
Joanna N. Kalatskaja
Keyword(s):  
Phytophthora Infestans ◽  
Transcriptional Activity ◽  
Hydrolytic Enzymes ◽  
Potato Cultivar ◽  
Leaf Damage ◽  
Potato Plants ◽  
Development Of Resistance ◽  
Potato Resistance ◽  
Inhibitor Genes ◽  
Late Blight Pathogen

The authors studied the effect of treatment with bacteria Bacillus subtilis Cohn (strains 26D) and B. thuringiensis Berliner (strain B-6066) on the hydrogen peroxide (H2O2) content, the activity of hydrolytic enzymes and their protein inhibitors in potato plants (Solanum tuberosum L.) in connection with development of resistance to the late blight pathogen - oomycete Phytophthora infestans Mont. de Bary. Studies were carried out on potato plants of the susceptible Early Rose potato cultivar that were treated with a suspension of B. subtilis and B. thuringiensis bacteria (108 cells/ml) and infected with P. infestans (107 spores/ml). A decrease in the degree of leaf damage by oomycete was revealed under the influence of the genus Bacillus bacteria, depending on the strain. The increase in potato resistance to P. infestans infection was mediated by the stimulating effect of the B. subtilis 26D and the B. thuringiensis B-6066 bacteria on the concentration of H2O2, the modulating effect on the activity of hydrolytic enzymes and the enhancement of the transcriptional activity of protease and amylase inhibitor genes in plant tissues. Differences in the degree of activation of the transcriptional activity of hydrolase inhibitor genes by the B. subtilis 26D and the B. thuringiensis B-6066 bacteria were revealed, which suggests differential ways of forming the potato resistance to P. infestans under their influence.

The influence of co-treatment with Bacillus thuringiensis B-5351 and salicylic acid on the resistance of potato plants to Phytophthora infestans (Mont.) de Bary

2021 ◽  
pp. 109-130
Author(s):  
Antonina V. Sorokan ◽  
◽  
Guzel F. Burkhanova ◽  
Valentin Yu. Alekseev ◽  
Igor V. Maksimov ◽  
...  
Keyword(s):  
Late Blight ◽  
Transcriptional Activity ◽  
Proteinase Inhibitors ◽  
Combined Treatment ◽  
Bacterial Suspension ◽  
Plant Tissues ◽  
Potato Plants ◽  
Intact Plants ◽  
Gene Transcripts ◽  
Late Blight Pathogen

Biocontrol agents based on strains of microorganisms that participate in mutualistic relationships with host plants, including those based on strains of endophytic bacteria, can be an alternative to chemical pesticides. There are endophytic B. thuringiensis strains that produce insect-toxic proteins and induce systemic resistance of plants to pathogens. An important issue is the possibility of regulation of the relationship of endophytic bacterial strains with the host-plant and their ability to induce plant defense reactions against pathogens by signaling molecules, including salicylic acid (SA). The aim of this work was to study the effect of SA and the endophytic bacterial strain B. thuringiensis B-5351 on the activity of potato plants reactions associated with signaling and biosynthesis of SA, as well as the activity of a potato trypsin inhibitor upon infection with the late blight pathogen. We used sterile test tube potato plants (Solanum tuberosum L.) of the Early Rose cultivar. 20-days-old potato plants were inoculated with a bacterial suspension of B. thuringiensis B-5351 (1*108 cells / ml) in distilled water, or in 1 μM SA solution by applying 5 μl of bacterial suspension to 4 upper leaves (20 μl / plant). Control plants were treated with distilled water, and some plants were treated with 1 μM SA solution in the same volume. On the 7th day after inoculation with B. thuringiensis B-5351, the plants were infected with oomycete P. infestans spores (5 μl of 1*105 spore/ ml suspension per leaf). To assay the damaged area, leaves were photographed; the images were analyzed using the ImageJ software. 24 h after infection with late blight pathogen spores, plants were fixed to assess the transcriptional activity of PR6, PR1 and PAL genes (See Table 1) (using CFX Connect Real-Time PCR Detection System Bio-Rad (USA)), and after 6, 24 and 48 h - to analyze the activity of plant proteinase inhibitors and the content of hydrogen peroxide. We estimated the number of colonyforming units (CFU) of microorganisms in plant tissues after their surface sterilization on the 7th day after inoculation of plants with B. thuringiensis B-5351 bacteria. The significance of the differences between the samples was accessed using Student’s t-test (p<0.05). Tables and Figures show data as the mean of the replicates and their standard deviations (M±SD). All experiments were conducted in three-five biological and three analytical replicates. In this work, we showed that SA presence increased the content of living cells of B. thuringiensis B-5351 bacteria in the internal tissues of plants (2,3±1,5×106 CFU/g) as compared to individual treatment with the bacterial strain under investigation (7,3±1,6×105 CFU/g) (See Table 2). Using the method of RAPD analysis, we established the identity of bacteria isolated from internal tissues of potato plants and the initial strain of B. thuringiensis B-5351 (See Fig. 1). Apparently, such titer of bacterial population regulated by plant signaling molecules in plant tissues is an important factor in the formation of plant resistance. Thus, we did not observe a decrease in the area of late blight symptoms on the leaves of potato plants treated only with B. thuringiensis B-5351, while under the individual action of SA this parameter decreased by half, and upon combined treatment of plants with SA and bacteria B. thuringiensis B-5351 - almost 4 times as compared to water-treated plants (See Fig. 2). Treatment with bacteria B. thuringiensis B-5351 increased the activity of proteinase inhibitors in potato plants only 6 h after infection with the late blight pathogen; in plants treated with bacteria cells of B. thuringiensis B-5351 together with SA, there was an almost a twofold increase in the activity of proteinase inhibitors after 6 and 24 h after infection with oomycete P. infestans. Under the influence of SA, a 15-20% increase in the content of H2O2 in intact plants was observed in all variants. In infected plants treated with SA, the H2O2 content exceeded the control values by more than 40%. In plants treated with a suspension of bacteria B. thuringiensis B-5351 and SA plants, the presence of the late blight pathogen caused a threefold increase in the H2O2 content 6 h after infection (See Fig. 3). In the variant with combined treatment with SA and bacteria B. thuringiensis B-5351 of noninfected plants, the content of PR6 gene transcripts exceeded the control level by 25%, as in infected plants treated with SA. Treatment of plants with SA together with bacteria B. thuringiensis B-5351 increased the number of PR6 gene transcripts by more than 50% relative to water-treated non-infected plants (See Fig. 4). In this work, we found that the strain of endophytic bacteria under investigation promoted the accumulation of transcripts of genes encoding PR1 and PAL proteins to the same extent as SA, both individually and when used together. In the presence of the late blight pathogen, more than a twofold (relative to this level in water-treated intact plants) increase in the content of PAL gene mRNA in potato plants was observed in cases of both individual and combined application of SA and B. thuringiensis B-5351 cells. Thus, treatment with B. thuringiensis B-5351 bacteria together with SA induces salicylate-dependent defense reactions (PR1, PAL), as well as an increase in the transcriptional activity of the PR6 gene, which, according to the literature, is a jasmonate-dependent gene. Our results showed the effectiveness of the composition based on live bacteria B. thuringiensis B-5351 and SA in protecting potato plants from late blight by inducing systemic defense responses in plants. Taking into account the insecticidal activity of the strain under investigation against Russian wheat aphids and Colorado potato beetle, which we previously described, the possibility of increasing plant resistance to late blight by biocontrol agent combining B. thuringiensis B-5351 with SA can open one of the approaches to the development of multifunctional protection of plants from biotic influences.

Endophytic bacteria Bacillus subtilis 26D reduce the damage to potato plants by late blight, stimulating the transcriptional activity of jasmonate-dependent genes

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 398-403
Author(s):  
A.V. Sorokan ◽  
G.F. Burkhanova ◽  
I.V. Maksimov
Keyword(s):  
Bacillus Subtilis ◽  
Jasmonic Acid ◽  
Late Blight ◽  
Endophytic Bacteria ◽  
Transcriptional Activity ◽  
Acid Biosynthesis ◽  
Anionic Peroxidase ◽  
Potato Plants ◽  
Protective Genes ◽  
Late Blight Pathogen

Bacillus subtilis 26D stimulated transcriptional activity of jasmonic acid biosynthesis genes and jasmonate-dependent protective genes, including anionic peroxidase involved in lignification, which increased their resistance to late blight pathogen.

scholarly journals Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type

2017 ◽  
Vol 2 (1) ◽  
pp. 90-91
Author(s):  
Virupaksh U. Patil ◽  
G. Vanishree ◽  
Debasis Pattanayak ◽  
Sanjeev Sharma ◽  
Vinay Bhardwaj ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Potato Late Blight ◽  
Complete Mitogenome ◽  
Late Blight Pathogen

scholarly journals EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans

2017 ◽  
Vol 12 (4) ◽  
pp. e1300733 ◽  
Author(s):  
Soriya Rin ◽  
Yuri Mizuno ◽  
Yusuke Shibata ◽  
Mayuka Fushimi ◽  
Shinpei Katou ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Nicotiana Benthamiana ◽  
Potato Late Blight ◽  
Late Blight Pathogen
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