scholarly journals The influence of cultivation conditions of promising Bacillus subtilis strains on their ability to produce antifungal metabolites

2020 ◽  
Author(s):  
T. M. Sidorova ◽  
A. M. Asaturova ◽  
A. I. Khomyak
Keyword(s):  
Bacillus Subtilis ◽  
Nutrient Medium ◽  
Antifungal Compounds ◽  
Cultivation Temperature ◽  
Cultivation Conditions ◽  
Iturin A ◽  
Antifungal Metabolites ◽  
Medium Acidity

Antifungal compounds, including surfactin and iturin A, are accumulated by B. subtilis BZR336g strain at the cultivation temperature of 20.0-25.0 °C and the nutrient medium acidity pH8.0, for B. subtilis BZR517 strain these parameters are 30.0-35.0 °C and pH8.0-10.0, respectively.

scholarly journals Optimization of laboratory cultivation conditions for the synthesis of antifungal metabolites by bacillus subtilis strains

2020 ◽  
Vol 27 (7) ◽  
pp. 1879-1885
Author(s):  
Tatyana M. Sidorova ◽  
Anzhela M. Asaturova ◽  
Anna I. Homyak ◽  
Natalya A. Zhevnova ◽  
Margarita V. Shternshis ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cultivation Conditions ◽  
Antifungal Metabolites

scholarly journals Physiological and biochemical aspects of the fungicidal action of promising biocontrol Bacillus subtilis strains against phytopathogenic fungi pp. Fusarium and Pyrenophora

2020 ◽  
Vol 21 ◽  
pp. 00016
Author(s):  
Marina Pavlova ◽  
Anzhela Asaturova ◽  
Valeria Allakhverdian ◽  
Tatyana Sidorova
Keyword(s):  
Bacillus Subtilis ◽  
Culture Fluid ◽  
Phytopathogenic Fungi ◽  
Fungicidal Action ◽  
Iturin A ◽  
Antifungal Metabolites ◽  
Physiological And Biochemical

The article presents some aspects of the interaction between biocontrol Bacillus subtilis strains and phytopathogenic fungi Fusarium and Pyrenophora. The presence of antifungal metabolites complexes in the culture fluid of the strains, including surfactin and iturin A has been found. The nature of the changes in the mycelium of phytopathogenic fungi is examined when co-cultivated with B.subtilis strains.

Cloning, Sequencing, and Characterization of the Genetic Region Relevant to Biosynthesis of the Lipopeptides Iturin A and Surfactin in Bacillus subtilis

2003 ◽  
Vol 47 (4) ◽  
pp. 272-277 ◽  
Author(s):  
Shiyi Yao ◽  
Xuewen Gao ◽  
Norbert Fuchsbauer ◽  
Wolfgang Hillen ◽  
Joachim Vater ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Iturin A ◽  
Genetic Region

Influence of the Production of Two Lipopeptides, Iturin A and Surfactin S1, on Oxygen Transfer During Bacillus subtilis Fermentation

1996 ◽  
pp. 571-579
Author(s):  
Choukri Hbid ◽  
Philippe Jacques ◽  
Hary Razafindralambo ◽  
Mpanya Kalonda Mpoyo ◽  
Eric Meurice ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oxygen Transfer ◽  
Iturin A

scholarly journals Bacillus spp.; perspectiva de su efecto biocontrolador mediante antibiosis en cultivos afectados por fitopatógenos

Nova ◽  
2011 ◽  
Vol 9 (16) ◽  
pp. 177 ◽  
Author(s):  
Cristian Layton ◽  
Edna Maldonado ◽  
Luisa Monroy ◽  
Lucía Constanza Corrales Ramírez MSC ◽  
Ligia Consuelo Sánchez Leal MSC
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Bacillus Sp ◽  
Bacillus Brevis ◽  
Iturin A ◽  
Bacillus Spp

El presente estudio documental evalúa el efecto biocontrolador del género <em>Bacillus sp </em>contra hongos fitopatógenos de plantas, particularmente, a través de relaciones antagónicas inductoras de muerte celular en términos inminentemente naturales. <em>Fusarium oxysporum </em>se encuentra muy relacionado con casos de marchitez vascular y pudrición de raíz en variedad de plantas, obstrucción de los vasos que permiten la circulación vegetal hasta causar amarillamiento de las hojas por imposibilidad en el transporte de nutrientes, causal de grandes pérdidas económicas en el campo agrícola nacional. Se han establecido varios mecanismos para controlar este hongo micelial dentro de las que se encuentran el uso extensivo y variable de agroquímicos y pesticidas, práctica que por sus efectos nocivos con el medio ambiente se ha comenzado a reemplazar por empleo de especies del género <em>Bacillus. </em>La acción biocontroladora de este género bacteriano esta mediada por su perfil bioquímico ya que son productores de múltiples metabolitos biológicamente activos, en el caso de <em>Bacillus subtilis </em>de Iturin A y fengycin y en <em>Bacillus brevis de gramicidina S </em>(1-5) son capaces de inhibir el desarrollo y crecimiento normal de otros microorganismos, lo que sugiere su utilización para el biocontrol de plagas en aras al fortalecimiento de los actuales estándares de calidad en los procesos ambientales.

scholarly journals Activity of Fengycin and Iturin A Isolated From Bacillus subtilis Z-14 on Gaeumannomyces graminis Var. tritici and Soil Microbial Diversity

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiawen Xiao ◽  
Xiaojun Guo ◽  
Xinlei Qiao ◽  
Xuechao Zhang ◽  
Xiaomeng Chen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Relative Abundance ◽  
Gaeumannomyces Graminis ◽  
Control Effect ◽  
Soil Bacterial Diversity ◽  
Soil Microbial Diversity ◽  
Wheat Seedlings ◽  
Iturin A ◽  
Soil Microbial ◽  
Take All

Bacillus subtilis Z-14 can inhibit phytopathogenic fungi, and is used as a biocontrol agent for wheat take-all disease. The present study used the soil-borne fungus Gaeumannomyces graminis var. tritici (Ggt), which causes wheat take-all disease, and the soil microbial community as indicators, and investigated the antifungal effects of fengycin and iturin A purified from strain Z-14 using high performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, respectively. The results showed that fengycin destroyed the internal structure of Ggt cells by digesting the cytoplasm and organelles, forming vacuoles, and inducing hyphal shrinkage and distortion. Iturin A induced cell wall disappearance, membrane degeneration, intracellular material shrinkage, and hyphal fragmentation. A biocontrol test demonstrated a 100% control effect on wheat take-all when wheat seedlings were treated with fengycin at 100 μg/ml or iturin A at 500 μg/ml. Iturin A and fengycin both reduced the relative abundance of Aspergillus and Gibberella. At the genus level, iturin A reduced the relative abundance of Mortierella and Myrothecium, while fengycin reduced that of Fusarium. Only fengycin treatment for 7 days had a significant effect on soil bacterial diversity.

scholarly journals Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

2022 ◽  
Vol 8 (1) ◽  
pp. 80
Author(s):  
Yongmei Li ◽  
Mengyuan Xia ◽  
Pengbo He ◽  
Qiaoming Yang ◽  
Yixin Wu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Management Strategies ◽  
Citrus Fruit ◽  
Inhibition Zone ◽  
Penicillium Digitatum ◽  
Antifungal Compounds ◽  
Fruit Peel ◽  
Post Harvest

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.

Effect of cell culture models on the evaluation of anticancer activity and mechanism analysis of the potential bioactive compound, iturin A, produced by Bacillus subtilis

2019 ◽  
Vol 10 (3) ◽  
pp. 1478-1489 ◽  
Author(s):  
Haobin Zhao ◽  
Xixi Zhao ◽  
Shuzhen Lei ◽  
Yawen Zhang ◽  
Dongyan Shao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bacillus Subtilis ◽  
3D Culture ◽  
Bioactive Compound ◽  
Mechanism Analysis ◽  
Iturin A ◽  
Culture Cells ◽  
Culture Models ◽  
Cell Culture Models ◽  
Cells Cultured

Compared to 2D culture, cells cultured in 3D culture were more resistant to iturin A from Bacillus.

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