scholarly journals Features of the initial stages of the relationship between barley, phytopathogenic fungus Fusarium culmorum and rhizobacteria Pseudomonas fluorescens 2137

2020 ◽  
Author(s):  
A. I. Shaposhnikov ◽  
N. A. Vishnevskaya ◽  
V. Yu. Shakhnazarova ◽  
D. S. Syrova ◽  
E. V. Borodina ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Root Exudates ◽  
Plant Defence ◽  
Fusarium Culmorum ◽  
Phytopathogenic Fungus ◽  
The Relationship

It was shown that enhanced colonization of barley's roots by Fusarium culmorum in the presence of Pseudomonas fluorescens 2137 may be due to the composition of root exudates. Strain 2137 can enhance expression of plant defence gene PAL.

scholarly journals The Role of Root Exudates of Barley Colonized by Pseudomonas fluorescens in Enhancing Root Colonization by Fusarium culmorum

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 366
Author(s):  
Nadezhda Vishnevskaya ◽  
Vlada Shakhnazarova ◽  
Alexander Shaposhnikov ◽  
Olga Strunnikova
Keyword(s):  
Amino Acids ◽  
Pseudomonas Fluorescens ◽  
Root Exudates ◽  
Fusarium Culmorum ◽  
Ultra Performance Liquid Chromatography ◽  
Root Surface ◽  
Phytopathogenic Fungus ◽  
Quantitative Composition ◽  
Experimental Conditions ◽  
In The Beginning

The aim of this study was to find out why after joint inoculation of the substrate with the phytopathogenic fungus Fusarium culmorum and the antagonistic bacterium Pseudomonas fluorescens the amount of the fungus on the root surface in the beginning of the colonization was greater on the roots colonized by the bacterium than on control roots. This phenomenon is especially interesting because joint inoculation with P. fluorescens was always followed by a considerable decrease in the incidence of Fusarium root rot. In two experiments barley was grown in sterile vermiculite inoculated only with F. culmorum, only with P. fluorescens and jointly with the fungus and the bacterium. In the control, vermiculite was not inoculated with any microorganisms. After the removal from the vermiculite, barley plants were transferred into deionized water for the collection of root exudates. The duration of barley growth in the vermiculite and in the water was different in the two experiments. The exudates were tested for their ability to elicit chemotropism in F. culmorum and influence its growth. We did not observe any chemotropism of F. culmorum towards barley root exudates. However, the exudates of the barley colonized by the bacterium stimulated the growth of fungal germ tubes. Using an ultra-performance liquid chromatography system, we found that experimental conditions influenced the quantitative composition of the exudates. The amount of amino acids in the solution of exudates decreased considerably after a prolonged growth of control barley in water, while the presence of P. fluorescens resulted in a considerably increase of the amount of amino acids in the exudates. The exudates of barley colonized by P. fluorescens contained much more glucose, lactic acid and several amino acids than the exudates of control barley. These components are known to be necessary for the growth of F. culmorum. Their presence in the exudates of barley colonized by P. fluorescens seems to be the reason of a more active colonization by the fungus of barley roots colonized by the bacterium.

Activation of protective reactions in barley plants during colonization of roots with the phytopathogenic fungus Fusarium culmorum in the presence of Pseudomonas fluorescens 2137

2020 ◽  
Author(s):  
A.I. Shaposhnikov ◽  
◽  
N.A. Vishnevskaya ◽  
V.Yu. Shakhnazarova ◽  
D.S. Syrova ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fusarium Culmorum ◽  
Phytopathogenic Fungus ◽  
Protective Response ◽  
Host Protection

The expression of the PAL gene, one of the host protection genes, in sterile barley plants and colonized F. culmorum and P. fluorescens 2137 were assessed. The obtained results indicate that strain 2137 may cause a more active protective response (1.5-2.1 fold) in barley than a phytopathogenic fungus.

Aromatic Carboxylic Acids in Barley-Root Exudates and Their Influence on the Growth of Fusarium culmorum and Pseudomonas fluorescens

2020 ◽  
Vol 56 (3) ◽  
pp. 344-351 ◽  
Author(s):  
A. I. Shaposhnikov ◽  
V. Yu. Shakhnazarova ◽  
N. A. Vishnevskaya ◽  
E. V. Borodina ◽  
O. K. Strunnikova
Keyword(s):  
Pseudomonas Fluorescens ◽  
Carboxylic Acids ◽  
Root Exudates ◽  
Fusarium Culmorum ◽  
Barley Root ◽  
Aromatic Carboxylic Acids

Ароматические карбоновые кислоты корневых экссудатов ячменя и их влияние на рост Fusarium culmorum и Pseudomonas fluorescens

2020 ◽  
Vol 56 (3) ◽  
pp. 292-300
Author(s):  
А. И. Шапошников ◽  
В. Ю. Шахназарова ◽  
Н. А. Вишневская ◽  
Е. В. Бородина ◽  
О. К. Струнникова
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fusarium Culmorum

scholarly journals Accumulation of cinnamic acid and vanillin in eggplant root exudates and the relationship with continuous cropping obstacle

2011 ◽  
Vol 10 (14) ◽  
pp. 2659-2665 ◽  
Author(s):  
Chen Shaoli ◽  
Zhou Baoli ◽  
Lin Shanshan ◽  
Li Xia ◽  
Ye Xueling
Keyword(s):  
Root Exudates ◽  
Cinnamic Acid ◽  
Continuous Cropping ◽  
The Relationship

scholarly journals Pheromone-Induced G2 Arrest in the Phytopathogenic Fungus Ustilago maydis

2003 ◽  
Vol 2 (3) ◽  
pp. 494-500 ◽  
Author(s):  
Tatiana García-Muse ◽  
Gero Steinberg ◽  
José Pérez-Martín
Keyword(s):  
Cell Cycle ◽  
Ustilago Maydis ◽  
Phytopathogenic Fungus ◽  
Microtubule Organization ◽  
Mating Response ◽  
Cycle Arrest ◽  
Synthetic Pheromone ◽  
Genes Encoding ◽  
The Relationship ◽  
Corn Smut

ABSTRACT In the corn smut fungus Ustilago maydis, pathogenic development is initiated when two compatible haploid cells fuse and form the infectious dikaryon. Mating is dependent on pheromone recognition by compatible cells. In this report, we set out to evaluate the relationship between the cell cycle and the pheromone response in U. maydis. To achieve this, we designed a haploid pheromone-responsive strain that is able to faithfully reproduce the native mating response in nutrient-rich medium. Addition of synthetic pheromone to the responsive strain induces the formation of mating structures, and this response is abolished by mutations in genes encoding components of the pheromone signal transduction cascade. After recognition of pheromone, U. maydis cells arrest the cell cycle in a postreplicative stage. Visualization of the nucleus and microtubule organization indicates that the arrest takes place at the G2 phase. Chemical-induced cell cycle arrest and release in the presence of pheromone further support this conclusion.

The influence of soils with different textures on development, colonization capacity and interactions between Fusarium culmorum and Pseudomonas fluorescens in soil and on barley roots

2014 ◽  
Vol 389 (1-2) ◽  
pp. 131-144 ◽  
Author(s):  
Olga K. Strunnikova ◽  
Nadezhda A. Vishnevskaya ◽  
Anastasia S. Ruchiy ◽  
Vlada Yu. Shakhnazarova ◽  
Nikolai I. Vorobyov ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fusarium Culmorum

scholarly journals Modelling the relationship between environmental factors, transcriptional genes and deoxynivalenol mycotoxin production by strains of two Fusarium species

2010 ◽  
Vol 8 (54) ◽  
pp. 117-126 ◽  
Author(s):  
M. Schmidt-Heydt ◽  
R. Parra ◽  
R. Geisen ◽  
N. Magan
Keyword(s):  
Environmental Factors ◽  
Control Strategies ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Mycotoxin Production ◽  
Contour Maps ◽  
Complex Interactions ◽  
Time Changes ◽  
First Time ◽  
The Relationship

The effect of changes in temperature/water activity ( a w ) on growth, deoxynivalenol (DON) production and trichothecene gene cluster expression (18 genes) for strains of Fusarium culmorum and Fusarium graminearum was studied. The expression data for six key transcription genes ( TRI4, TRI5 , TRI6, TRI10, TRI12 and TRI13 ) were analysed using multiple regression analyses to model the relationship between these various factors for the first time. Changes in a w and temperature significantly ( p = 0.05) affected growth and DON. Microarray data on expression of these genes were significantly related to DON production for both strains. Multi-regression analysis was done and polynomial models found to best fit the relationship between actual/predicted DON production relative to the expression of these TRI genes and environmental factors. This allowed prediction of the amounts of DON produced in two-dimensional contour maps to relate expression of these genes to either a w or temperature. These results suggest complex interactions between gene expression ( TRI genes), environmental factors and mycotoxin production. This is a powerful tool for understanding the role of these genes in relation to environmental factors and enables more effective targeted control strategies to be developed.

Extracellular proteinases from the phytopathogenic fungus Fusarium culmorum

2006 ◽  
Vol 42 (3) ◽  
pp. 298-303 ◽  
Author(s):  
E. V. Ievleva ◽  
T. A. Revina ◽  
N. N. Kudryavtseva ◽  
A. V. Sof’in ◽  
T. A. Valueva
Keyword(s):  
Fusarium Culmorum ◽  
Phytopathogenic Fungus ◽  
Extracellular Proteinases
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