scholarly journals Antifungal activity of lipopeptides from endophytic strains of the genus Bacillus sp. against the fungus Stagonospora nodorum (Berk.)

2020 ◽  
Author(s):  
E. R. Sarvarova ◽  
E. A. Cherepanova ◽  
I. V. Maksimov
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Pathogenic Fungus ◽  
Stagonospora Nodorum ◽  
Bacillus Sp ◽  
Genus Bacillus ◽  
Antibiotic Effect

The direct antibiotic effect of lipopeptides from four endophytic strains on the germination of spores of the pathogenic fungus Stagonospora nodorum (Berk.) was found and the minimum inhibitory concentration (MIC) of these lipopeptides was determined.

scholarly journals Two Symmetrical Squarylium Cyanine Dyes: Synthesis, Photophysics and Antifungal Activity in Saccharomyces cerevisiae

2020 ◽  
Vol 3 (1) ◽  
pp. 106
Author(s):  
Vanessa S. D. Gomes ◽  
João C. C. Ferreira ◽  
Renato E. F. Boto ◽  
Paulo Almeida ◽  
Maria João M. F. Sousa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Emission Spectroscopy ◽  
Inhibitory Concentration ◽  
Analytical Techniques ◽  
Cyanine Dyes ◽  
Absorption And Emission ◽  
Nir Absorption ◽  
Absorption And Emission Spectroscopy

Two squarylium cyanine dyes were synthesized and characterized by the usual analytical techniques, including Vis-NIR absorption and emission spectroscopy. Their antifungal activity was evaluated, through the obtention of minimum inhibitory concentration (MIC) values, using yeasts of the species Saccharomyces cerevisiae as a biological model.

ANTIFUNGAL TOPICAL GEL OF LEAF EXTRACT OF AMARANTHUS VIRIDIS L. FOR TREATMENT OF CUTANEOUS CANDIDIASIS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 39-44
Author(s):  
A. P. Pandit ◽  
◽  
K. S. Khandagale ◽  
V. C Nakhate ◽  
N. N Dharmadhikari
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
High Viscosity ◽  
Good Choice ◽  
Zone Of Inhibition ◽  
Minimum Fungicidal Concentration ◽  
Topical Gel ◽  
Cutaneous Candidiasis

The objective of the study was to prepare antifungal gel using leaves of Amaranthus viridis for the treatment of cutaneous candidiasis. The leaves were studied for pharmacognostic evaluation. The powder of leaves was tested for phytoconstituents. The plant extract was evaluated for the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and antifungal activity. Gel was prepared and evaluated for pH, viscosity, homogeneity and grittiness. MIC and MFC of extract were both found to be 600 mg. The zone of inhibition of extract was obtained at 21.2±0.2mm, which confirmed antifungal activity, due to presence of phenolic compound. Gel exhibited good antifungal activity, good spreadability, extrudability and high viscosity. Thus, gel loaded with leaves extract of A. viridis is a good choice for the treatment of cutaneous candidiasis.

scholarly journals Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A–C and Their Antimicrobial Activity

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2653 ◽  
Author(s):  
José Cheel ◽  
Jan Hájek ◽  
Marek Kuzma ◽  
Kumar Saurav ◽  
Iva Smýkalová ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Pathogenic Fungus ◽  
High Yield ◽  
Preparative Hplc ◽  
Efficient Tool ◽  
Cyclic Lipopeptides ◽  
Potential Applications ◽  
Polymeric Resins ◽  
Weak Antibacterial Activity

Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, Desmonostoc muscorum CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (1–3), which were further repurified by semi-preparative HPLC, affording 1, 2, and 3, with a purity of 86%, 92%, and 90%, respectively. The chemical identities of 2–3 were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas 1 was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data. Owing to the high yield (50 mg), compound 2 was broadly screened for its antimicrobial potential exhibiting a strong antifungal activity against Alternaria alternata, Monographella cucumerina, and Aspergillus fumigatus, with minimum inhibitory concentration (MIC) values of 0.58, 2.34, and 2.34 µg/mL; respectively, and weak antibacterial activity against Bacillus subtilis with a MIC value of 37.5 µg/mL. Compounds 1 and 3 were tested only against the plant pathogenic fungus Sclerotinia sclerotiorum due to their low yield, displaying a moderate antifungal activity. The developed chromatographic method proved to be an efficient tool for obtaining muscotoxins with potent antifungal properties.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

In vitro antifungal activity of luliconazole against nondermatophytic moulds

2019 ◽  
Vol 58 (5) ◽  
pp. 703-706 ◽  
Author(s):  
Jun Maeda ◽  
Hiroyasu Koga ◽  
Kou Yuasa ◽  
Daisuke Neki ◽  
Yasuko Nanjoh ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Drug Resistant ◽  
Fusarium Spp ◽  
Microdilution Method ◽  
In Vitro Antifungal Activity

Abstract In vitro antifungal activity of luliconazole against nondermatophytic moulds causing superficial infections was compared with that of five classes of 12 topical and systemic drugs. The minimum inhibitory concentration (MIC) of the drugs against the genera of Neoscytalidium, Fusarium, Aspergillus, Scedosporium, and Alternaria was measured via modified microdilution method. In results, the nondermatophytic moulds were found to be less susceptible to drugs to which Neoscytalidium spp. and Fusarium spp. were typically drug resistant. However, luliconazole was effective against all the genera tested, including afore-mentioned two species, and had the lowest MICs among the drugs tested.

scholarly journals Inclusion Complexes of Concentrated Orange Oils and β-Cyclodextrin: Physicochemical and Biological Characterizations

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5109
Author(s):  
Cynthia Torres-Alvarez ◽  
Sandra Castillo ◽  
Eduardo Sánchez-García ◽  
Carlos Aguilera González ◽  
Sergio Arturo Galindo-Rodríguez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inclusion Complexes ◽  
Inhibitory Concentration ◽  
Entrapment Efficiency ◽  
Ft Ir ◽  
Ir Analysis

Concentrated orange oils (5x, 10x, 20x) are ingredients used in different industries as components of flavors and aromas due to their great organoleptic qualities. This research focuses on the search for alternative uses for their application through encapsulation in inclusion complexes with β-cyclodextrin (β–CD). Inclusion complexes of concentrated orange oils (COEO) and β–CD were developed by the co-precipitated method in ratios of 4:96, 12:88, and 16:84 (w/w, COEO: β–CD). The best powder recovery was in the ratio 16:84 for the three oils, with values between 82% and 84.8%. The 20x oil in relation 12:88 showed the highest entrapment efficiency (89.5%) with 102.3 mg/g of β–CD. The FT-IR analysis may suggest an interaction between the oil and the β–CD. The best antioxidant activity was observed in the ratio 12:88 for the three oils. The antifungal activity was determined for all the inclusion complexes, and the 10x fraction showed the highest inhibition at a concentration of 10 mg/mL in ratios 12:88 and 16:84. Antibacterial activity was determined by the minimum inhibitory concentration (MIC) and was found at a concentration of 1.25 mg/mL in ratios 12:88 and 16:84 for 5x and 20x oils.

scholarly journals Antifungal Activity of Essential Oil ofEucalyptus camaldulensisDehnh. against SelectedFusariumspp.

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Martin Muthee Gakuubi ◽  
Angeline W. Maina ◽  
John M. Wagacha
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Inhibitory Concentration ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Minimum Fungicidal Concentration ◽  
Potential Use

The objective of this study was to evaluate the antifungal activity of essential oil (EO) ofEucalyptus camaldulensisDehnh. against fiveFusariumspp. commonly associated with maize.The essential oil had been extracted by steam distillation in a modified Clevenger-type apparatus from leaves ofE. camaldulensisand their chemical composition characterized by gas chromatography mass spectrometry. Poisoned food technique was used to determine the percentage inhibition of mycelial growth, minimum inhibitory concentration, and minimum fungicidal concentration of the EO on the test pathogens. Antifungal activity of different concentrations of the EO was evaluated using disc diffusion method. The most abundant compounds identified in the EO were 1,8-cineole (16.2%),α-pinene (15.6%),α-phellandrene (10.0%), and p-cymene (8.1%). The EO produced complete mycelial growth inhibition in all the test pathogens at a concentration of 7-8 μL/mL after five days of incubation. The minimum inhibitory concentration and minimum fungicidal concentration of the EO on the test fungi were in the range of 7-8 μL/mL and 8–10 μL/mL, respectively. These findings confirm the fungicidal properties ofE. camaldulensisessential oils and their potential use in the management of economically importantFusariumspp. and as possible alternatives to synthetic fungicides.

scholarly journals Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Fresh Water Fish ◽  
Minimum Fungicidal Concentration ◽  
Kaempferia Galanga ◽  
Activity Test

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

Phytochemical Screening and Antifungal Activity of Kulantrillo De Allambre (Adiantum capillus-veneris) Extract as a Formulated Vaginal Cream Against Candida albicans

10.17158/234 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Kathleen G. Bersabal ◽  
Kyrie Marie D. Deluao ◽  
Meg C. Ibarra ◽  
Nessa Karla C. Jerusalem
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Physical Properties ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Antifungal Property ◽  
Vaginal Cream ◽  
Adiantum Capillus Veneris

Candida albicans is a fungus that is normally present on the skin and in mucous membranes such as vagina, mouth, or rectum. This fungus also can travel through the blood stream and affect the throat, intestines, and heart valves. It becomes an infectious agent when there is some change in the body environment that allows it to grow out of control; thus, causing a modern day disease brought on by antibiotics, drugs, processed foods, sugar and poor diet. Folkloric practice suggests that Kulantrillo de Allambre (Adiantum capillus-veneris) is effective as antifungal medication against Candida albicans; therefore, this study was undertaken to verify such claim. This effort included the phytochemical screening of active constituents, susceptibility and minimum inhibitory concentration tests involving the plant. To prove that Kulantrillo de Allambre possesses antifungal activity, a formulation of vaginal cream out of the plant extracts was tested. Alkaloids, saponins, steroids, flavonoids, tannins and anthraquinones were performed following the method described by Guevara et al. (2005). Kirby-Bauer Method was employed for the susceptibility test and two-fold agar dilution was employed for the MIC. Vaginal cream was formulated following the method of Ansel (2004). Tests revealed the presence of flavonoids only, which exhibit the antifungal property. Kirby-Bauer Method revealed that both test extract and positive control showed strong inhibition equivalent to 17.71 ± 6.3183 mm and 23.54 ± 4.9629 mm, respectively. Minimum Inhibitory Concentration of the plant extract was 50,000 ug/mL. Statistical analysis showed that there is no significant difference between the antifungal property of plant extract and positive control. Both test drugs have similar antifungal property, suggesting that the plant material is a good antifungal agent. The physical properties of vaginal cream conformed to the standard of physical properties of commercialized product available in the market.

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