scholarly journals In vitro evaluation of some halophilic bacterial isolates as biofertilizers

2020 ◽  
Author(s):  
E. A. Samsonova ◽  
I. M. Ibrahim ◽  
Yu. P. Fedonenko ◽  
S. A. Konnova
Keyword(s):  
Heavy Metal ◽  
Nitrogen Fixation ◽  
Halophilic Bacteria ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Bacterial Isolates ◽  
In Vitro Evaluation

For six strains of halophilic bacteria, nitrogen fixation ability, production of phytohormones and siderophores, the solubilization of phosphates, and heavy metal resistance were revealed.

scholarly journals Bioremediation of lead using bacterial isolates and study their plant relieving effect on wheat under metal contamination

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Prashakha J. Shukla ◽  
Vishwa R. Vyas
Keyword(s):  
Heavy Metal ◽  
Stress Condition ◽  
Inhibitory Concentration ◽  
Anthropogenic Activities ◽  
Wheat Plant ◽  
Cost Effective ◽  
Heavy Metal Resistance ◽  
Metal Resistance

Increasing concentration of heavy metals due to various anthropogenic activities is a serious problem. To overcome this issue, many chemical and physical methods are available but they are either directly or indirectly harmful to nature. By these methods more quality of chemicals are wasted. So, bioremediation is the best method to remove pollutants. It is an eco-friendly and cost-effective process. A low concentration of heavy metal is required to plant for their growth and metabolic process but at higher concentration, plants do not survive. With the use of microbes, we can survive plants at certain levels. During this work heavy metal tolerating microorganism was isolated and purified. Various tests were performed like staining, minimum inhibitory concentration, multiple heavy metal resistance, multiple antibiotic resistance, biochemical test, DNA isolation, in vitro examination of the wheat plant under the stress condition of lead (1000ppm).

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

scholarly journals Analysis of the Genome of the Heavy Metal Resistant and Hydrocarbon-Degrading Rhizospheric Pseudomonas qingdaonensis ZCR6 Strain and Assessment of Its Plant-Growth-Promoting Traits

2021 ◽  
Vol 23 (1) ◽  
pp. 214
Author(s):  
Daria Chlebek ◽  
Tomasz Płociniczak ◽  
Sara Gobetti ◽  
Agata Kumor ◽  
Katarzyna Hupert-Kocurek ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Growth Promotion ◽  
Sulfur Metabolism ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Minimal Inhibitory Concentrations ◽  
Plant Growth Promoting

The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3(PO4)2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5, 15, and 10 mM metal, respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis.

scholarly journals Response of the callus cells of fir (Abies nordmanniana) to in vitro heavy metal stress

2017 ◽  
Vol 59 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Katarzyna Nawrot-Chorabik
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Stress ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Stress Factors ◽  
Negative Effects ◽  
Abies Nordmanniana ◽  
Long Time

Abstract The aim of the presented research was to investigate the effect of three heavy metals - lead, cadmium and copper - on the callus cells of Abies nordmanniana. The toxicity degree and toxicity effect of the selected heavy metals was determined on the embryonic level. On the basis of the spectrometric analyses as well as macroscopic and microscopic observations, this research referred to the accumulation of heavy metals in tissues, assuming that this mechanism is related to the acquisition of tolerance by cells exposed to this type of abiotic stress. Moreover, the effect of the genotype of fir on the cell defence, that is, the induction of tolerance, was analysed. Understanding of the issues related to the heavy metal resistance of plant genotypes in future may contribute to the selection of genotypes of individuals that are more resistant to stress factors, particularly in the multi-directional and rational forest management. The results showed that lead (20 mg l-1), which proved to be the most toxic amongst the three examined heavy metals, has the most severe negative effects on the tissue of fir trees. Copper (20 mg l-1) was accumulated for a long time in the cells of fir trees, and it was not degraded or excreted outside the tissues even after three weeks of in vitro culture. Of the three tested genotypes, G14 had the greatest tendency to accumulate each of the examined metals, that is, it appeared to be the least tolerant genotype.

scholarly journals Antibiotic and Heavy Metal Resistance of Bacteria Isolated from Diseased Mud Crab (Scylla serrata)

2015 ◽  
Vol 3 (3) ◽  
pp. 1-15
Author(s):  
Cik Syahrizawati M Z. ◽  
Ikhwanuddin M. ◽  
Wendy W. ◽  
Zulhisyam AK ◽  
Lee SW
Keyword(s):  
Heavy Metal ◽  
Vibrio Parahaemolyticus ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Edwardsiella Tarda ◽  
Scylla Serrata ◽  
Bacterial Isolates ◽  
Mud Crab ◽  
Salmonella Spp ◽  
Klebsiella Spp

A total of 320 bacteria isolated from marketable size and diseased mud crab (Scylla serrata) at a commercial farm. The isolated bacteria were Aeromonas spp. n = 70, Edwardsiella tarda n = 50, Vibrio alginolyticus n = 40, Vibrio parahaemolyticus n = 20, Salmonella spp. n = 70 and Klebsiella spp. n = 70. All the bacterial isolates were tested for antibiotic susceptibility against 16 types of antibiotics by using disk diffusion method. The antibiotics tested in this study were nalidixic acid (30 ?g/disk), oxolinic acid (2 ?g/disk), compound sulphonamides (300 ?g/disk), doxycycline (30 ?g/disk), tetracycline (30 ?g/disk), novobiocin (30 ?g/disk), chloramphenicol (30 ?g/disk), kanamycin (30 ?g/disk), sulphamethoxazole (25 ?g/disk), flumequine (30 ?g/disk), erythromycin (15 ?g/disk), ampicillin (10 ?g/disk), spiramycin (100 ?g/disk), oxytetracycline (30 ?g/disk), amoxycillin (25 ?g/disk) and fosfomycin (50 ?g/disk). Heavy metal resistance pattern of the present bacterial isolates was also characterized against mercury (Hg2+), chromium (Cr6+), copper (Cu2+), and Zinc (Zn2+) by using two fold agar dilution method. The percentage of antibiotic sensitivity of the present bacterial isolates was ranged from 12.5 % to 100 % in which most of the present bacteria isolates were not sensitive to ampicillin whereas all the bacteria isolates were sensitive to nalidixic acid, flumequine and oxytetracycline. Overall, the total of antibiotic sensitive case was reported as 72.7% whereas antibiotic resistance and intermediate sensitive case was recorded as 19.7% and 7.4 %, respectively. The Multiple Antibiotic Resistance (MAR) values were range of 0.03 to 0.29 in which Aeromonas spp (0.29) showed the highest value of MAR. This was followed by Salmonella spp. (0.21) and Klebsiella spp. (0.21), Edwardsiella tarda (0.20), Vibrio alginolyticus (0.09) and Vibrio parahaemolyticus (0.03). The MAR value indicated that the commercial S. serrata were not contaminated to the test antibiotics. Furthermore, low resistance activity of the present bacterial isolates to the tested heavy metals (Cr6+: 20.7 % to 30.8 %, Zn2+: 0 % to 40 %, Cu2+: 18.8 % to 25 % and Hg2+: 30 % to 33.3 %) was observed.

scholarly journals Heavy metal resistance properties of bacteria from different soil types in Horo Guduru Wollega, Ethiopia

2019 ◽  
Vol 5 (11) ◽  
pp. 320
Author(s):  
Melkamu T. Addisu ◽  
Adugna M. Bikila
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Agricultural Soils ◽  
Environmental Concern ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Soil Types ◽  
Resistant Bacteria ◽  
Bacterial Isolates

<p class="abstract"><strong>Background:</strong> The quality of life on earth is linked inseparably to the overall quality of the environment. Soil pollution with heavy metals has become a critical environmental concern due to its potential adverse ecological effects. The study explored the heavy metals resistance properties of bacteria isolated from fertilizer applied agricultural and non-agricultural soils.</p><p class="abstract"><strong>Methods:</strong> The soil samples were collected from both fertilizer applied agricultural soils and non-agricultural soils. After identification and characterization of the isolates from both soil types, six (6) similar bacterial isolates were selected to screen for resistance against Cobalt (Co<sup>+</sup>), Lead (Pb<sup>2+</sup>), Cromium (Cr<sup>+3</sup>), Mercury (Hg<sup>2+</sup>), Nickel (Ni<sup>2+</sup>), Cadmium (Cd<sup>2+</sup>) and Zinc (Zn<sup>2+</sup>) heavy metals. The minimum inhibitory concentration (MIC) for the bacterial isolates were determined by gradually increasing the concentration of heavy metals on agar plates until the isolates failed to show growth.  </p><p class="abstract"><strong>Results:</strong> The isolates from fertilizer applied agricultural soil showed the highest resistance against the selected heavy metals than those isolated from fertilizers not applied (nonagricultural) soils.</p><p><strong>Conclusions:</strong> From this result it can be seen that fertilizer has significant role in influencing the heavy metal resistance properties of bacteria and these heavy metal resistant bacteria can be useful for the bioremediation of heavy metal contaminated environment. </p>

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