scholarly journals In vitro cultivation, aquaculture and methods of transformation of water caltrop Trapa L.

2020 ◽  
Author(s):  
M. A. Panfilova ◽  
E. V. Mikhaylova ◽  
Kh. G. Musin ◽  
B. R. Kuluev
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Biolistic Transformation ◽  
In Vitro Cultivation ◽  
Optimal Conditions

In vitro and aquarium cultures of water caltrop were obtained. Optimal conditions of cultivation were determined. The cultures were subjected to Agrobacterium rhizogenes-mediated and biolistic transformation.

scholarly journals Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

2021 ◽  
Vol 209 (06) ◽  
pp. 43-52
Author(s):  
Marina Markova ◽  
Elena Somova
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Optimal Time ◽  
In Vitro Cultivation ◽  
Initiation Time ◽  
Multiplication Factor ◽  
Optimal Conditions ◽  
Prunus Domestica ◽  
Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

scholarly journals Mycelial growth and sporulation of Apoharknessia eucalyptorum on different culture media

2019 ◽  
Vol 45 (3) ◽  
pp. 272-278
Author(s):  
Alexandre Techy de Almeida Garrett ◽  
Álvaro Figueredo Santos ◽  
Afonso Figueiredo Filho ◽  
Evandro Vagner Tambarussi ◽  
Arthur Birais Gonçalves ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
In Vitro Cultivation ◽  
Malt Extract ◽  
Optimal Conditions ◽  
Southern Brazil ◽  
Eucalyptus Dunnii ◽  
White Mycelium

ABSTRACT Apoharknessia eucalyptorum was first described in 2017 and identified on leaves of Eucalyptus dunnii in Southern Brazil. However, information about in vitro cultivation for complementary studies is lacking. In the present study, leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media: malt extract agar (MEA); potato dextrose agar (PDA); V8 juice agar (V8); and bean dextrose agar (BEAN), under constant lighting. Apoharknessia eucalyptorum caused leaf blight on the inoculated leaves. The best conditions for mycelial growth were at 25°C on PDA, BEAN and MEA. Considering sporulation, optimal conditions were 25°C for all tested media and 20°C for PDA and BEAN. Colony characteristics changed with temperature; at 15°C colonies formed a fluffy mycelium, whereas at 25°C mycelium spread across the medium forming dark margins lined by dirty-white mycelium and conidia. The conditions indicated for in vitro growth and sporulation of A. eucalyptorum are the culture media MEA, PDA and BEAN at 25°C

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris
Keyword(s):  
Treponema Pallidum ◽  
In Vitro Cultivation

scholarly journals What quantitative mechanical loading stimulates in vitro cultivation best?

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Jerry Natenstedt ◽  
Aimee C Kok ◽  
Jenny Dankelman ◽  
Gabrielle JM Tuijthof
Keyword(s):  
Mechanical Loading ◽  
In Vitro Cultivation

scholarly journals Acanthamoeba spp. as Agents of Disease in Humans

2003 ◽  
Vol 16 (2) ◽  
pp. 273-307 ◽  
Author(s):  
Francine Marciano-Cabral ◽  
Guy Cabral
Keyword(s):  
Cerebrospinal Fluid ◽  
Cutaneous Lesion ◽  
Laboratory Diagnosis ◽  
Immune Defense ◽  
In Vitro Cultivation ◽  
Cutaneous Lesions ◽  
Trophozoite Stage ◽  
Biopsy Material ◽  
Pathogenic Potential

SUMMARY Acanthamoeba spp. are free-living amebae that inhabit a variety of air, soil, and water environments. However, these amebae can also act as opportunistic as well as nonopportunistic pathogens. They are the causative agents of granulomatous amebic encephalitis and amebic keratitis and have been associated with cutaneous lesions and sinusitis. Immuno compromised individuals, including AIDS patients, are particularly susceptible to infections with Acanthamoeba. The immune defense mechanisms that operate against Acanthamoeba have not been well characterized, but it has been proposed that both innate and acquired immunity play a role. The ameba's life cycle includes an active feeding trophozoite stage and a dormant cyst stage. Trophozoites feed on bacteria, yeast, and algae. However, both trophozoites and cysts can retain viable bacteria and may serve as reservoirs for bacteria with human pathogenic potential. Diagnosis of infection includes direct microscopy of wet mounts of cerebrospinal fluid or stained smears of cerebrospinal fluid sediment, light or electron microscopy of tissues, in vitro cultivation of Acanthamoeba, and histological assessment of frozen or paraffin-embedded sections of brain or cutaneous lesion biopsy material. Immunocytochemistry, chemifluorescent dye staining, PCR, and analysis of DNA sequence variation also have been employed for laboratory diagnosis. Treatment of Acanthamoeba infections has met with mixed results. However, chlorhexidine gluconate, alone or in combination with propamidene isethionate, is effective in some patients. Furthermore, effective treatment is complicated since patients may present with underlying disease and Acanthamoeba infection may not be recognized. Since an increase in the number of cases of Acanthamoeba infections has occurred worldwide, these protozoa have become increasingly important as agents of human disease.

Sign in / Sign up

Export Citation Format

Share Document