scholarly journals Studying of fertilization-independent embryo- and endospermogenesis in maize embryo sacs

2020 ◽  
Author(s):  
M. I. Chumakov ◽  
I. V. Volokhina ◽  
Yu. S. Gusev ◽  
S. I. Mazilov ◽  
V. V. Fadeev ◽  
...  
Keyword(s):  
Experimental Analysis ◽  
Genes Expression ◽  
Before And After ◽  
Maize Embryo

The literature and experimental analysis of maize embryo and endospermogenesis genes expression before and after pollination will be presented. Data on the CRISPR/Cas9 editing of maize embryo- and endospermogenesis genes are discussed.

STUDY OF CXCL12, CCR4, EGFR GENE EXPRESSION IN MIGRATING MYELOMONOBLASTIC LEUKEMIA CELLS BEFORE AND AFTER CHEMOTHERAPY

2019 ◽  
Vol 1 (1) ◽  
pp. 100-104
Author(s):  
A. B. Filina ◽  
O. A. Svitich ◽  
Yu. I. Ammur ◽  
A. K. Golenkov ◽  
E. F. Klinushkina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Mononuclear Cells ◽  
Boyden Chamber ◽  
External Stimulation ◽  
Healthy Donors ◽  
Genes Expression ◽  
Before And After ◽  
Chemokine Cxcl12 ◽  
And Migration ◽  
Cell Chemotaxis

Аim. A study of CXCL12 effect on the migration of mononuclear cells isolated from healthy patients, from patients with myelomonoblastic leukemia before and after chemotherapy and the study of CCR4, EGFR and CXCL12 genes expression after exposure to CXCL12. Materials and methods. The chemotaxis of mononuclear cells (MNCs) of healthy donors and patients with myelomonoblastic leukemia was studied in a Boyden chamber, followed by isolation of RNA, reverse transcription and PCR-RV. Results. A significant increase in myelomonoblasic cell chemotaxis towards CXCL12 after chemotherapy was demonstrated, as well as a decrease in the expression of this chemokine in tumor cells before chemotherapy after exposure to CXCL12. Сonclusion. Presumably, the tumor cells themselves produce CXCL12 in large amounts, which is necessary for the disturbance of intercellular interactions and further intravasation, whose production may decrease with external stimulation by the same chemokine. CXCL12 also helps to increase the expression level of EGFR and CCR4, which leads to increased tumor proliferation and migration of tumor cells.

scholarly journals Expression of Longevity Genes Induced by a Low-Dose Fluvastatin and Valsartan Combination with the Potential to Prevent/Treat “Aging-Related Disorders”

2019 ◽  
Vol 20 (8) ◽  
pp. 1844 ◽  
Author(s):  
Miodrag Janić ◽  
Mojca Lunder ◽  
Srdjan Novaković ◽  
Petra Škerl ◽  
Mišo Šabovič
Keyword(s):  
Low Dose ◽  
Telomerase Activity ◽  
Treatment Discontinuation ◽  
Placebo Control ◽  
Middle Aged ◽  
Beneficial Effects ◽  
Genes Expression ◽  
Longevity Genes ◽  
Before And After ◽  
Stored Blood

The incidence of aging-related disorders may be decreased through strategies influencing the expression of longevity genes. Although numerous approaches have been suggested, no effective, safe, and easily applicable approach is yet available. Efficacy of low-dose fluvastatin and valsartan, separately or in combination, on the expression of the longevity genes in middle-aged males, was assessed. Stored blood samples from 130 apparently healthy middle-aged males treated with fluvastatin (10 mg daily), valsartan (20 mg daily), fluvastatin-valsartan combination (10 and 20 mg, respectively), and placebo (control) were analyzed. They were taken before and after 30 days of treatment and, additionally, five months after treatment discontinuation. The expression of the following longevity genes was assessed: SIRT1, PRKAA, KLOTHO, NFE2L2, mTOR, and NF-κB. Treatment with fluvastatin and valsartan in combination significantly increased the expression of SIRT1 (1.8-fold; p < 0.0001), PRKAA (1.5-fold; p = 0.262) and KLOTHO (1.7-fold; p < 0.0001), but not NFE2L2, mTOR and NF-κB. Both fluvastatin and valsartan alone significantly, but to a lesser extent, increased the expression of SIRT1, and did not influence the expression of other genes. Five months after treatment discontinuation, genes expression decreased to the basal levels. In addition, analysis with previously obtained results revealed significant correlation between SIRT1 and both increased telomerase activity and improved arterial wall characteristics. We showed that low-dose fluvastatin and valsartan, separately and in combination, substantially increase expression of SIRT1, PRKAA, and KLOTHO genes, which may be attributed to their so far unreported pleiotropic beneficial effects. This approach could be used for prevention of ageing (and longevity genes)–related disorders.

scholarly journals Positive Regulation of Macromolecule Metabolic Process Belongs to the Main Mechanisms Crucial for Porcine Oocytes Maturation

2017 ◽  
Vol 5 (1) ◽  
pp. 15-31 ◽  
Author(s):  
Wiesława Kranc ◽  
Piotr Celichowski ◽  
Joanna Budna ◽  
Ronza Khozmi ◽  
Artur Bryja ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Metabolic Process ◽  
Developmental Competence ◽  
Positive Regulation ◽  
Genes Expression ◽  
Before And After ◽  
Genes Encoding ◽  
Compound Process ◽  
Maturational Stage

SummaryThe mammalian oocytes maturation is the compound process that involves morphological and molecular changes. These modifications include storage of macromolecules, which are crucial for proteins biosynthesis during periimplantation stages of embryo development. This study was aimed to investigate the genes expression profile encoding macromolecules important for regulation of proper porcine oocytes maturation.The porcine oocytes were collected from large ovarian follicles and analyzed both before and after in vitro maturation (IVM). Additionally, to check the developmental competence status, brilliant crezyl blue test (BCB) was performed. The obtained cDNA was used for biotin labeling and fragmentation by AffymetrixGeneChip® WT Terminal Labeling and Hybridization (Affymetrix). The preliminary analysis of the scanned chips was performed using AffymetrixGeneAtlasTM Operating Software. The created CEL files were imported into downstream data analysis software.In results, we found expression of 419 different genes, 379 genes were down-regulated and 40 genes were up-regulated in relation to the oocyte transcriptome before in vitro procedure. We observed up-regulation of all genes involved in “positive regulation of macromolecule metabolic process” before IVM as compared to transcriptional profile analyzed after IVM.In conclusion, we suggested that genes encoding proteins involved in macromolecule metabolism are important for achieving of porcine oocytes maturational stage. Moreover, the “activity of macromolecules metabolism” is much more increased in immature oocytes.

scholarly journals Knockdown of ANGPTL4 May Reduce the Expression of LPL during Differentiation of 3T3-L1 Preadipocyte

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhao X ◽  
◽  
Zhang F ◽  
Liu Y ◽  
Liu H ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Energy Homeostasis ◽  
Adipocyte Differentiation ◽  
Adipocyte Size ◽  
Preadipocyte Differentiation ◽  
Similar Expression Pattern ◽  
Genes Expression ◽  
Before And After ◽  
Ppar Signaling

Adipose tissue is a complex, highly metabolically active tissue. The expansion of adipose tissue is mainly affected by two processes: the number of adipocytes and the change of adipocyte size. Adipocyte differentiation is regulated by multiple factors. 3T3-L1 preadipocyte line has become the most widely studied cell line for adipocyte proliferation, differentiation and regulation of related genes. In our previous study, we found that ANGPTL4 was related to preadipocyte differentiation through PPAR signaling pathway. Recent studies have shown that ANGPTL4 has functions of regulating oncogenesis, vascular permeability, glucose homeostasis, lipid metabolism, energy homeostasis. In this study, we observed the changes of key genes expression before and after inhibiting ANGPTL4 to explore how ANGPTL4 influence on lipid metabolism. Results shows that, ANGPTL4 and LPL have similar expression pattern. We suspect that, ANGPTL4 and LPL may form a pathway, when ANGPTL4 is suppressed it may block the pathway and inhibit LPL.

Angiogenic biomaker study in osteosarcoma

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e21507-e21507
Author(s):  
P. C. Pavoni-Ferreira ◽  
A. S. Petrilli ◽  
M. T. Alves ◽  
R. Jesus-Garcia Filho ◽  
S. R. Toledo
Keyword(s):  
Metronomic Chemotherapy ◽  
High Dose Chemotherapy ◽  
Marker Genes ◽  
High Dose ◽  
Lower Grade ◽  
Vegf Receptor ◽  
Blood Samples ◽  
Genes Expression ◽  
Before And After ◽  
Prospective Assessment

e21507 Background: This study represents a prospective assessment of angiogenesis genes mRNA expression in tumors and blood from patients treated with pre- and post-operative Brazilian osteosarcoma protocol (GCBTO 2006) that introduce metronomic chemotherapy (anti-angiogenic) in order to try to increase survival of osteosarcoma patients. Methods: Tumor samples from 27 patients were analyzed before and after chemotherapy to determine VEGFA, VEGFR1, VEGFR2, PDGFC, SDF1 and TSP1 genes expression profile by Quantitative Real Time PCR. Also, blood samples of these patients were investigated pre- and post-chemotherapy and at the end of high-dose chemotherapy trying to evaluate potential for proangiogenic factors and antiangiogenic factor (TSP1) which could be used to monitor treatment activity. Results: Of all six genes studied pre- and post- chemotherapy, in tumor samples, only SDF1 and VEGFR2 were underexpressed. SDF1 gene has the lowest expression at all. In tumor samples, TSP1 and VEGFA expression was higher than SDF1, VEGFR2 and PDGFC expression in biopsies and surgeries (P=0.001). VEGFR1 expression was higher than VEGFR2 expression (P=0.001). PDGFC and VEGFR1 overexpression were associated with necrosis grade I and II (Huvos score) (P=0.005). VEGFA and TSP1 were overexpressed in 96% and 92% of surgery samples, respectively. In blood samples from biopsy, surgery and end of treatment there were no statistically significant changes in the marker genes expression. Conclusions: The study suggests an association between PDGFC and VEGFR1 overexpression and lower grade necrosis. TSP1 and VEGFA were the most expressed genes in all tumor samples but TSP1 was lower than VEGFA in biopsies and VEGFA was lower than TSP1 in surgery (P=0.001). Although VEGFR2 is the primary receptor of VEGF, VEGFR1 was the most expressed VEGF receptor. No significant financial relationships to disclose.

Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shaghayegh Pishkari ◽  
Razie Hadavi ◽  
Ameneh Koochaki ◽  
Javad Razaviyan ◽  
Mahdi Paryan ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Medullary Thyroid Carcinoma ◽  
Target Genes ◽  
Critical Role ◽  
Luciferase Assay ◽  
Medullary Thyroid ◽  
Genes Expression ◽  
Before And After ◽  
New Strategy

Abstract Objectives The aim of the present study was to investigate the expression of AXL and mTOR genes and their targeting microRNAs (miRNAs) including miR-34a and miR-144 in Medullary Thyroid Carcinoma (MTC) cell line, TT, and determine the effect of these two miRNAs on their target genes to introduce new molecular markers or therapeutics. Methods The expression of miR-34a, miR-144, and their targets genes including AXL and mTOR was evaluated by quantitative Real-time PCR. Luciferase assay was performed to confirm the interaction between miRNAs and their target mRNAs. The expression level of AXL and mTOR was evaluated before and after miRNAs induction in TT cell line compared with Cos7 as control cells. Results The expression of AXL and mTOR were up-regulated significantly, while miR-34a and miR-144 were down-regulated in TT cell line compared to Cos7. After transduction, the overexpression of miR-34a and 144 caused down-regulation of both genes. Luciferase assay results showed that the mTOR is targeted by miR-34a and miR-144 and the intensity of luciferase decreased in the presence of miRNAs. Conclusions Based on the results of the present study and since AXL and mTOR genes play a critical role in variety of human cancers, suppression of these genes by their targeting miRNAs, especially miR-34a and miR-144, can be propose as a new strategy for MTC management. However, more studies are needed to approve the hypothesis.

scholarly journals Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1328
Author(s):  
Ahmed H. Abed ◽  
Esraa F. Hegazy ◽  
Sherif A. Omar ◽  
Rehab M. Abd El-Baky ◽  
Ahmed A. El-Beih ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antimicrobial Effect ◽  
Multidrug Resistant ◽  
Haemolytic Activity ◽  
Bacterial Isolates ◽  
Phenotypic Properties ◽  
Genes Expression ◽  
Before And After ◽  
Mild Reduction ◽  
Staphylococcus Spp

Staphylococcus species cause diseases in animals and humans. The prevalence and antimicrobial profiles of Staphylococcus spp. in animals and human samples in the Minya Governorate, Egypt, were determined, and resistance- and virulence-associated genes were observed in multidrug-resistant (MDR) isolates. Moreover, the antibacterial effect of carvacrol essential oil (EO) on the MDR isolates was studied. A total of 216 samples were aseptically collected from subclinically mastitic cow’s milk (n = 100), sheep abscesses (n = 25) and humans (n = 91). Out of 216 samples, a total of 154 single Staphylococcus species (71.3%) were isolated. The most frequent bacterial isolates were S. aureus (43%), followed by S. schleiferi (25%), S. intermedius (12%), S. xylosus (12%), S. haemolyticus (4.5%), S. epidermidis (2%) and S. aurecularis (1%). Haemolytic activity and biofilm production were detected in 77 and 47% of isolates, respectively. Antimicrobial susceptibility testing showed a high degree of resistance to the most commonly used antimicrobials in human and veterinary practices. The mecA, vanA, vanC1 and ermC resistance genes were detected in 93, 42, 83 and 13% of isolates, respectively. Moreover, hla, icaA and icaD virulence genes were detected in 50, 75 and 78% of isolates, respectively. Carvacrol effectively inhibited the growth of all tested isolates at concentrations of 0.1, 0.05 and 0.04% while a concentration of 0.03% inhibited 75% of isolates. Interestingly, some phenotypic changes were observed upon treatment with a carvacrol oil concentration of 0.03%. All the treated MDR Staphylococcus isolates changed from multidrug resistant to either susceptible or intermediately susceptible to 2–3 antimicrobials more than parental bacterial isolates. Real-time PCR was applied for the detection of the differential expression of mecA and vanC1 genes before and after treatment with carvacrol which revealed a mild reduction in both genes’ expression after treatment. Staphylococcus spp. Containing MDR genes are more likely to spread between humans and animals. From these results, carvacrol EO is a promising natural alternative to conventional antimicrobials for pathogens impacting human health and agriculture due to its potential antimicrobial effect on MDR pathogens; even in sub-lethal doses, carvacrol EO can affect their phenotypic properties and genes’ expression.

scholarly journals Consumer preferences before and after a food safety scare: An experimental analysis of the 2010 egg recall

Food Policy ◽  
2017 ◽  
Vol 66 ◽  
pp. 25-34 ◽  
Author(s):  
Tongzhe Li ◽  
John C. Bernard ◽  
Zachary A. Johnston ◽  
Kent D. Messer ◽  
Harry M. Kaiser
Keyword(s):  
Food Safety ◽  
Experimental Analysis ◽  
Consumer Preferences ◽  
Before And After

Expression of genes coding for chromatin-modifying enzymes in maize embryo sacs before and after pollination

Plant Gene ◽  
2020 ◽  
Vol 22 ◽  
pp. 100221 ◽  
Author(s):  
Irina Volokhina ◽  
Yury Gusev ◽  
Yelizaveta Moiseeva ◽  
Vladimir Fadeev ◽  
Alla Kolesova ◽  
...  
Keyword(s):  
Expression Of Genes ◽  
Before And After ◽  
Maize Embryo

scholarly journals Association of High Cardiovascular Fitness and the Rate of Adaptation to Heat Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Małgorzata Żychowska ◽  
Alicja Nowak-Zaleska ◽  
Grzegorz Chruściński ◽  
Ryszard Zaleski ◽  
Jan Mieszkowski ◽  
...  
Keyword(s):  
Heat Stress ◽  
Cardiorespiratory Fitness ◽  
Adaptive Response ◽  
Interleukin 10 ◽  
Cardiovascular Fitness ◽  
Blood Samples ◽  
Qrt Pcr ◽  
Genes Expression ◽  
Before And After ◽  
Adaptation To Heat

This study aimed to compare changes in genes expression associated with inflammation and apoptosis in response to heat stress caused by sauna between people with varying cardiorespiratory fitness levels. We hypothesis that high cardiorespiratory level caused higher positive changes after four weeks of sauna bathing. Blood samples were taken at rest before and after the first and last sauna sessions and 48 hours after the last sauna session and used to assay HSP70 (HSPA1A), HSP27 (HSPB1), interleukin 6 (IL6), and interleukin 10 (IL10) genes expression in blood with quantitative real-time qRT-PCR. Overall, small decreases in rest values of HSPA1A and IL6 mRNA, increase in HSPB1 mRNA, and a significant increase in IL10 mRNA were observed after four weeks of exposure to heat stress. Our findings suggest that an adaptive response to heat stress (an anti-inflammatory response) occurs faster in people with higher cardiorespiratory fitness.

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