scholarly journals Concentration of Free Amino Acids in Blood Plasma of Dairy Cows with Developing Hepatic Steatosis

2000 ◽  
Vol 69 (2) ◽  
pp. 93-99 ◽  
Author(s):  
A. Pechová ◽  
J. Illek ◽  
I. Liška ◽  
R. Halouzka ◽  
L. Pavlata
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Dairy Cows ◽  
Hepatic Steatosis ◽  
Free Amino Acids ◽  
Free Amino ◽  
In Blood Plasma

scholarly journals Possibilities for prediction of congenital fetal malformations and chromosomal abnormalities based on the determination of the levels of free amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women

2020 ◽  
pp. 152-158
Author(s):  
L. N. Keda ◽  
A. V. Naumov ◽  
V. YU. Smirnov
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Pregnant Women ◽  
Free Amino Acids ◽  
Free Amino ◽  
Chromosomal Abnormalities ◽  
Group I ◽  
Fetal Malformations ◽  
In Blood Plasma

Objective: to determine the possibilities for prediction and diagnosis of congenital fetal malformations and chromosomal abnormalities on the basis of the study of the levels of amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women with pathological conditions requiring artificial termination of pregnancy. Material and methods. The content of free amino acids and their nitrogen-containing derivatives was studied in 104 pregnant women having congenital malformations and chromosomal abnormalities in their fetuses at 13-22 weeks` gestation (group I) and 25 women with physiological pregnancy (group II). The amino acid level was determined by the high-performance liquid chromatography method. Results. The levels of 14 out of the 26 studied amino acids in the blood plasma of the pregnant women of group I were statistically higher than those of the women in group II. ROC analysis was used to determine six amino acids (glycine, α-aminobutyric acid, hydroxylysine, glutamic acid, citrulline, serine) and their threshold values which with high accuracy (85.3 %) allow of predicting congenital fetal malformations and chromosomal abnormalities. A prognostic model making it possible to determine high probability of congenital fetal anomalies based on the determination of the concentration of 5-hydroxytryptophan, glycine, asparagine, and serine in blood plasma of pregnant women has been developed. Conclusion. The study of the levels of amino acids and their nitrogen-containing derivatives in plasma of pregnant women at 13-22 weeks` gestation can be used for prenatal diagnosis of congenital fetal malformations and chromosomal abnormalities, as well as used as an additional criterion for making the decision on the necessity for artificial termination of pregnancy upon fetal medical indications.

scholarly journals FREE AMINO ACIDS OF BLOOD PLASMA AS AN INTEGRAL PARAMETER OF METABOLIC DISORDERS IN A LONG-TERM INTAKE OF SMALL DOSES OF LEAD ACETATE INTO THE ORGANISM

2017 ◽  
pp. 67-71
Author(s):  
V. M. Sheybak ◽  
A. Y. Pavliukovets ◽  
V. Yu. Smirnov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Lead Acetate ◽  
Male Rats ◽  
Integral Parameter ◽  
In Blood Plasma

Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.

PSII-8 Free amino acids in blood plasma of beef cattle under induced oxidative stress by hydrogen peroxide

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 315-316
Author(s):  
Bethaney M Penrod ◽  
Corbin R Fornes ◽  
Thu Dinh ◽  
Daniel Rivera
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Hydrogen Peroxide ◽  
Blood Plasma ◽  
Repeated Measures ◽  
Free Amino Acids ◽  
Free Amino ◽  
Fixed Effects ◽  
Block Design ◽  
In Blood Plasma

Abstract This study examined the effects of oxidative stress induced by hydrogen peroxide on free amino acids in blood plasma from beef heifers (n = 18). Cattle were supplemented with ground corn and soybean hulls to 310 to 456 kg of BW while grazing cool and warm-season pastures and were evaluated for preliminary oxidative markers; afterward, they were shipped and finished at a commercial feedlot in Iowa. Animals were blocked into three groups based on principal component analysis of oxidative stress markers and two treatments of either 20 mg hydrogen peroxide/kg BW (OX, n = 9) or 10 mL of saline (CON, n = 9) were equally and randomly assigned to animals within each group. On the day before slaughter, the OX and CON treatments were administered intravenously through the jugular vein. Blood samples were collected immediately before and 90 min after treatment, centrifuged into plasma, aliquoted, snap-frozen in liquid nitrogen, and stored at -80°C. Free amino acids in plasma were derivatized by propyl chloroformate, extracted in isooctane, and determined by gas chromatography – mass spectrometry. The data were analyzed as a randomized complete block design with repeated measures with treatment, time, and their interaction as fixed effects. Twenty-seven amino acids were quantified and sarcosine concentration was 1.5 mM greater in OX plasma than in that of CON (P = 0.064). Leucine, threonine, proline, asparagine, methionine, and α-aminobutyric acid were greater pre-treatment (P ≤ 0.006); whereas cystine was greater at 90 min post-treatment (P = 0.019). Although the effects of hydrogen peroxide injection on free amino acids in blood plasma were minimal, an increase in sarcosine concentration has been reported as a marker of induced oxidative stress.

A collaborative study on the determination of free amino acids in blood plasma

1980 ◽  
Vol 31 (5) ◽  
pp. 474-480 ◽  
Author(s):  
Alwyn P. Williams ◽  
David Hewitt ◽  
John E. Cockburn ◽  
Dorothy A. Harris ◽  
R. Andrew Moore ◽  
...  
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Collaborative Study ◽  
In Blood Plasma

The Free Amino Acids in Blood Plasma of Children with Kwashiorkor and Marasmus

1962 ◽  
Vol 11 (5) ◽  
pp. 517-524 ◽  
Author(s):  
GUILLERMO ARROYAVE ◽  
DOROTHY WILSON ◽  
CARLOTA DE FUNES ◽  
MOISéS BéHAR
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
In Blood Plasma

scholarly journals The determination of free amino acids in blood plasma

1969 ◽  
Vol 111 (3) ◽  
pp. 21P-21P ◽  
Author(s):  
D. K. Tommel ◽  
J. F. G. Vliegenthart ◽  
J. F. Arens
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
In Blood Plasma
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