scholarly journals High-throughput screening of "Cell-internalizing monoclonal antibody", a potent anti-tumor drug delivery carrier for antibody-drug conjugate

2014 ◽  
Vol 29 (4) ◽  
pp. 366-367
Author(s):  
Mai Yoshikawa
Keyword(s):  
Drug Delivery ◽  
Monoclonal Antibody ◽  
High Throughput ◽  
High Throughput Screening ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Drug Delivery Carrier ◽  
Tumor Drug Delivery ◽  
Antibody Drug

High-Throughput Platform to Identify Antibody Conjugation Sites from Antibody–Drug Conjugate Libraries

2020 ◽  
Vol 31 (4) ◽  
pp. 1199-1208 ◽  
Author(s):  
Sayumi Yamazoe ◽  
Jason M. Hogan ◽  
Sean M. West ◽  
Xiaodi A. Deng ◽  
Srikanth Kotapati ◽  
...  
Keyword(s):  
High Throughput ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Antibody Conjugation ◽  
Antibody Drug

scholarly journals A novel humanized MUC1 antibody–drug conjugate for the treatment of trastuzumab-resistant breast cancer

2021 ◽  
Author(s):  
Guang Wu ◽  
Lan Li ◽  
Yuxin Qiu ◽  
Wei Sun ◽  
Tianhao Ren ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Monoclonal Antibody ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Xenograft Model ◽  
Therapeutic Drug ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Her2 Breast Cancer ◽  
Antibody Drug

Abstract Mucin 1 (MUC1) has been regarded as an ideal target for cancer treatment, since it is overexpressed in a variety of different cancers including the majority of breast cancer. However, there are still no approved monoclonal antibody drugs targeting MUC1. In this study, we generated a humanized MUC1 (HzMUC1) antibody from our previously developed MUC1 mouse monoclonal antibody that only recognizes MUC1 on the surface of tumor cells. Furthermore, an antibody–drug conjugate (ADC) was generated by conjugating HzMUC1 with monomethyl auristatin (MMAE), and the efficacy of HzMUC1-MMAE on the MUC1-positive HER2+ breast cancer in vitro and in ‘Xenograft’ model was tested. Results from western blot analysis and immunoprecipitation revealed that the HzMUC1 antibody did not recognize cell-free MUC1-N in sera from breast cancer patients. Confocal microscopy analysis showed that HzMUC1 antibody bound to MUC1 on the surface of breast cancer cells. Results from mapping experiments suggested that HzMUC1 may recognize an epitope present in the interaction region between MUC1-N and MUC1-C. Results from colony formation assay and flow cytometry demonstrated that HzMUC1-MMAE significantly inhibited cell growth by inducing G2/M cell cycle arrest and apoptosis in trastuzumab-resistant HER2-positive breast cancer cells. Meanwhile, HzMUC1-MMAE significantly reduced the growth of HCC1954 xenograft tumors by inhibiting cell proliferation and enhancing cell death. In conclusion, our results indicate that HzMUC1-ADC is a novel therapeutic drug that can overcome trastuzumab resistance of breast cancer. HzMUC1-ADC should also be an effective therapeutic drug for the treatment of different MUC1-positive cancers in clinic.

Targeting Chemotherapy for Digestive Cancer Using Monoclonal Antibody Drug Conjugate

1993 ◽  
pp. 115-120
Author(s):  
Toshio Takahashi ◽  
Toshiharu Yamaguchi ◽  
Kazuya Kitamura ◽  
Akinori Noguchi ◽  
Eigo Otsuji ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Digestive Cancer ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Antibody Drug

scholarly journals High-Throughput, Multispecies, Parallelized Plasma Stability Assay for the Determination and Characterization of Antibody–Drug Conjugate Aggregation and Drug Release

ACS Omega ◽  
2017 ◽  
Vol 2 (8) ◽  
pp. 4207-4215 ◽  
Author(s):  
Kenneth R. Durbin ◽  
M. Shannon Nottoli ◽  
Nathaniel D. Catron ◽  
Nicole Richwine ◽  
Gary J. Jenkins
Keyword(s):  
Drug Release ◽  
High Throughput ◽  
Plasma Stability ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Antibody Drug

scholarly journals Follow-up Study of Patients Treated with Monoclonal Antibody-Drug Conjugate: Report of 77 Cases with Colorectal Cancer

1993 ◽  
Vol 84 (9) ◽  
pp. 976-981 ◽  
Author(s):  
Toshio Takahashi ◽  
Toshiharu Yamaguchi ◽  
Kazuya Kitamura ◽  
Akinori Noguchi ◽  
Mitsuyo Honda ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Monoclonal Antibody ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Follow Up Study ◽  
Antibody Drug
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