scholarly journals Enhancement effect of menthoxypropandiol and menthol on the penetration of indomethacin through Yucatan micropig skin in vitro.

1997 ◽  
Vol 12 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Makiko Fujii ◽  
Shizuka Yamanouchi ◽  
Kazumi Nagakura ◽  
Yasuhiro Takeda ◽  
Mitsuo Matsumoto
Keyword(s):  
Enhancement Effect ◽  
Yucatan Micropig

Enhancement Effect ofp‐Menthane‐3,8‐diol on In Vitro Permeation of Antipyrine and Indomethacin Through Yucatan Micropig Skin

2004 ◽  
Vol 30 (6) ◽  
pp. 673-677 ◽  
Author(s):  
Makiko Fujii ◽  
Yasuhiro Takeda ◽  
Minako Yoshida ◽  
Mitsuo Matsumoto ◽  
Yoshiteru Watanabe
Keyword(s):  
Enhancement Effect ◽  
In Vitro Permeation ◽  
Yucatan Micropig

scholarly journals Asialoglycoprotein Receptor-Targeted Superparamagnetic Perfluorooctylbromide Nanoparticles

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Yang Li ◽  
Chao Feng Yang ◽  
Hui Zuo ◽  
Ao Li ◽  
Sushant Kumar Das ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Rat Liver ◽  
Liver Parenchyma ◽  
Asialoglycoprotein Receptor ◽  
Enhancement Effect ◽  
Receptor Imaging ◽  
Significant Difference ◽  
Mean Size

Background. The decrease in asialoglycoprotein receptor (ASGPR) levels is observed in patients with chronic liver disease and liver tumor. The aim of our study was to develop ASGPR-targeted superparamagnetic perfluorooctylbromide nanoparticles (M-PFONP) and wonder whether this composite agent could target buffalo rat liver (BRL) cells in vitro and could improve R2 ∗ value of the rat liver parenchyma after its injection in vivo. Methods. GalPLL, a ligand of ASGPR, was synthesized by reductive amination. ASGPR-targeted M-PFOBNP was prepared by a film hydration method coupled with sonication. Several analytical methods were used to investigate the characterization and safety of the contrast agent in vitro. The in vivo MR T2 ∗ mapping was performed to evaluate the enhancement effect in rat liver. Results. The optimum concentration of Fe3O4 nanoparticles inclusion in GalPLL/M-PFOBNP was about 52.79 µg/mL, and the mean size was 285.6 ± 4.6 nm. The specificity of GalPLL/M-PFOBNP for ASGPR was confirmed by incubation experiment with fluorescence microscopy. The methyl thiazolyl tetrazolium (MTT) test showed that there was no significant difference in the optical density (OD) of cells incubated with all GalPLL/M-PFOBNP concentrations. Compared with M-PFOBNP, the increase in R2 ∗ value of the rat liver parenchyma after GalPLL/M-PFOBNP injection was higher. Conclusions. GalPLL/M-PFOBNP may potentially serve as a liver-targeted contrast agent for MR receptor imaging.

scholarly journals Effects of HSPA8, an evolutionarily conserved oviductal protein, on boar and bull spermatozoa

Reproduction ◽  
2009 ◽  
Vol 137 (2) ◽  
pp. 191-203 ◽  
Author(s):  
Roslyn M A Elliott ◽  
Rhiannon E Lloyd ◽  
Alireza Fazeli ◽  
Edita Sostaric ◽  
A Stephen Georgiou ◽  
...  
Keyword(s):  
Soluble Protein ◽  
Protein Fraction ◽  
Mammalian Species ◽  
Surface Proteins ◽  
Apical Plasma Membrane ◽  
Enhancement Effect ◽  
Soluble Protein Fraction ◽  
Sperm Survival ◽  
Pre Treatment

Previous studies have shown that a soluble protein fraction derived from preparations of apical plasma membrane (APM) of the oviductal epithelium enhances the in vitro survival of mammalian spermatozoa. Here, we show that the survival enhancing property of the soluble protein fraction seems to depend significantly upon heat shock 70 kDa protein 8 (HSPA8 previously known as HSPA10). The following findings in the present study enabled us to draw this conclusion: first, using proteomic analysis, we identified a subset of 70 kDa oviductal surface proteins that bound to spermatozoa, one of which was HSPA8. Second, pre-treatment of the soluble protein fraction with anti-HSPA8 antibody reduced the 24 h (at 39 °C) sperm survival enhancement effect normally induced by the presence of 200 μg/ml soluble APM proteins. Third, complementary experiments showed that substituting the soluble protein fraction with bovine recombinant HSPA8 (0.5–2 μg/ml) also elicited the sperm survival effect. Finally, we also tested the effect of bovine recombinant HSPA8 on bull spermatozoa and found similar, dose-responsive, sperm survival promoting effects. The conserved nature of HSPA8 between mammalian species suggests that this protein may represent a common biological mechanism for the maintenance of sperm survival in the oviduct.

scholarly journals Impact of Sodium N-[8-(2-Hydroxybenzoyl)amino]-caprylate on Intestinal Permeability for Notoginsenoside R1 and Salvianolic Acids in Caco-2 Cells Transport and Rat Pharmacokinetics

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2990 ◽  
Author(s):  
Ying Li ◽  
Dandan Yang ◽  
Chunyan Zhu
Keyword(s):  
Membrane Permeability ◽  
Cell Monolayer ◽  
Salvianolic Acid B ◽  
Absorption Enhancement ◽  
Enhancement Effect ◽  
Absorption Enhancers ◽  
Salvianolic Acid ◽  
Salvianolic Acids

For drugs with high hydrophilicity and poor membrane permeability, absorption enhancers can promote membrane permeability and improve oral bioavailability. Sodium N-[8-(2-hydroxybenzoyl)amino]caprylate (SNAC) is a new kind of absorption enhancer that has good safety. To investigate the absorption enhancement effect of SNAC on non-polar charged and polar charged drugs and establish the absorption enhancement mechanism of SNAC, SNAC was synthesized and characterized. Two representative hydrophilic drugs—notoginsenoside R1 (R1) and salvianolic acids (SAs)—were selected as model drugs. In vitro Caco-2 cells transport and in vivo rat pharmacokinetics studies were conducted to examine the permeation effect of SNAC on R1 and SAs. R1, rosmarinic acid (RA), salvianolic acid B (SA-B) and salvianolic acid B (SA-A) were determined to compare the permeation enhancement of different drugs. The MTT assay results showed that SNAC had no toxicity to Caco-2 cells. The transepithelial electrical resistance (TEER) of Caco-2 cell monolayer displayed that SNAC facilitated passive transport of polar charged SAs through the membrane of epithelial enterocytes. The pharmacokinetics results demonstrated that area under the curve (AUC) of RA, SA-B and SA-A with administration of SAs containing SNAC was 35.27, 8.72 and 9.23 times than administration of SAs. Tmax of RA, SA-B and SA-A were also prolonged. The AUC of R1 with administration of R1 containing SNAC was 2.24-times than administration of R1. SNAC is more effective in promoting absorption of SAs than R1. The study demonstrated that SNAC significantly improved bioavailability of R1 and SAs. What’s more, the effect of SNAC on absorption enhancement of charged drugs was larger than that of non-charged drugs. The current findings not only confirm the usefulness of SNAC for the improved delivery of R1 and SAs but also demonstrate the importance of biopharmaceutics characterization in the dosage form development of drugs.

Gadolinium-Based Nanoparticles Can Overcome the Radioresistance of Head and Neck Squamous Cell Carcinoma Through the Induction of Autophagy

2020 ◽  
Vol 16 (1) ◽  
pp. 111-124 ◽  
Author(s):  
S. Simonet ◽  
C. Rodriguez-Lafrasse ◽  
D. Beal ◽  
S. Gerbaud ◽  
C. Malesys ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Cell Uptake ◽  
Enhancement Effect ◽  
Additional Information ◽  
New Formulation

Radiation therapy is a mainstay in the therapeutic management of Head and Neck Squamous Cell Carcinoma (HNSCC). Despite significant progress in this field, radioresistance still accounts for most treatment failures. Gadolinium-based nanoparticles (GBNs) have shown great promises as radiosensitizers but the underlying sensitizing mechanism is still largely unknown with regards to the disparities obtained in in vitro studies. In this study, we show that a new formulation of GBNs, AGuIX®, can radiosensitize HNSCC after cell uptake and further accumulation in lysosomes. Although radiation alone triggered late apoptosis and mitochondrial impairment, the pre-treatment with GBNs led to complex DNA damage and a specific increase of autophagic cell death. In addition, a significant radio-enhancement effect was obtained after the pre-conditioning of cells with a glutathione inhibitor before GBNs treatment and radiation exposure. Overall, our results provide additional information on the radio-enhancing properties of GBNs in the management of radioresistant HNSCC.

scholarly journals Evaluation of Yucatan Micropig Skin for Use as an in Vitro Model for Skin Permeation Study.

1997 ◽  
Vol 20 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Makiko FUJII ◽  
Shizuka YAMANOUCHI ◽  
Naohide HORI ◽  
Noriko IWANAGA ◽  
Naruko KAWAGUCHI ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Skin Permeation ◽  
Permeation Study ◽  
Vitro Model ◽  
Yucatan Micropig

scholarly journals Evaluation of in vitro percutaneous enhancement effect of papain and pequi oil on diclofenac sodium permeation through human skin

2008 ◽  
Vol 44 (2) ◽  
Author(s):  
Patrícia Santos Lopes ◽  
Claudinéia Aparecida Sales de Olive Pinto ◽  
André Rolim Baby ◽  
Maria Valéria Robles Velasco ◽  
Maria Elena Taqueda ◽  
...  
Keyword(s):  
Human Skin ◽  
Diclofenac Sodium ◽  
Enhancement Effect

Sex of co-twin affects the in vitro developmental competence of oocytes derived from 6- to 8-week-old lambs

2017 ◽  
Vol 29 (7) ◽  
pp. 1379 ◽  
Author(s):  
Jennifer M. Kelly ◽  
David O. Kleemann ◽  
Hayley McGrice ◽  
Jose A. Len ◽  
Karen L. Kind ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Least Square ◽  
Developmental Competence ◽  
In Vitro Fertilisation ◽  
Enhancement Effect ◽  
Limited Evidence ◽  
Intrinsic Factors ◽  
The Impact ◽  
Birth Type

Several intrinsic factors (age, genotype, liveweight) influence the reliability of juvenile in vitro fertilisation embryo transfer (JIVET) programs. Limited evidence indicates that variability between lambs is reduced in twin-born lambs. We examined the impact of birth type (single, twin, triplet) and sex of the co-twin (with age, birthweight and liveweight as covariates) on JIVET outcomes. Birth type did not influence any parameter studied. However, blastocysts produced, as a percentage of embryos cleaved or total cumulus–oocyte complexes collected, was higher (P < 0.05) for females born with a female co-twin (67.0 ± 6.1, 57.5 ± 6.0 respectively) compared with those born with a male co-twin (26.9 ± 6.5, 22.3 ± 6.2 respectively; least-square mean ± s.e.m.). Blastocyst rates for lambs born with a male co-twin did not differ significantly from lambs born either as singles (39.5 ± 6.7%, 34.6 ± 6.5% respectively) or triplets (43.1 ± 10.6%, 36.5 ± 10.3% respectively). Other parameters were not influenced by sex of the co-twin. These results are indicative of an enhancement effect of the female co-twin on oocyte development. From a practical perspective, selecting lambs for a JIVET program based on litter size and sex of the co-twin is warranted.

scholarly journals Variation Assessment for in Vitro Permeabilities through Yucatan Micropig Skin

2011 ◽  
Vol 34 (4) ◽  
pp. 555-561 ◽  
Author(s):  
Hiroyuki Takeuchi ◽  
Shuichi Terasaka ◽  
Takanobu Sakurai ◽  
Atsushi Furuya ◽  
Hidetoshi Urano ◽  
...  
Keyword(s):  
Variation Assessment ◽  
Yucatan Micropig
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