scholarly journals Evaluation of calcitonin gene-related peptide prolonged release tablet in vitro and in vivo.

1996 ◽  
Vol 11 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Toshihiko Kaminuma ◽  
Nobuhiko Ochiai ◽  
Ritsuko Ehama ◽  
Masahiro Tajima ◽  
Kazuo Watabe ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Prolonged Release ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Release Tablet

scholarly journals Evidence that α-Calcitonin Gene-Related Peptide Is a Neurohormone that Controls Systemic Lipid Availability and Utilization

Endocrinology ◽  
2007 ◽  
Vol 149 (1) ◽  
pp. 154-160 ◽  
Author(s):  
Rachel N. Danaher ◽  
Kerry M. Loomes ◽  
Bridget L. Leonard ◽  
Lynda Whiting ◽  
Debbie L. Hay ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Physiological Role ◽  
Calcitonin Gene Related Peptide ◽  
Physiological Regulation ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Motor Nerves ◽  
In Vivo Effects

α-Calcitonin gene-related peptide (αCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary physiological role has thus far remained obscure. Increased lipid content, particularly in skeletal muscle and liver, is strongly implicated in the pathogenesis of insulin resistance, but the physiological regulation of organ lipid is imperfectly understood. Here we report our systematic investigations of the effects of αCGRP on in vitro and in vivo indices of lipid metabolism. In rodents, levels of αCGRP similar to those in the blood markedly stimulated fatty acid β-oxidation and evoked concomitant mobilization of muscle lipid via receptor-mediated activation of muscle lipolysis. αCGRP exerted potent in vivo effects on lipid metabolism in muscle, liver, and the blood via receptor-mediated pathways. Studies with receptor antagonists were consistent with tonic regulation of lipid metabolism by an endogenous CGRP agonist. These data reveal that αCGRP is a newly recognized regulator of lipid availability and utilization in key tissues and that it may elevate the availability of intramyocellular free fatty acids to meet muscle energy requirements generated by contraction by evoking their release from endogenous triglyceride.

Comparison of the in vitro and in vivo pharmacology of adrenomedullin, calcitonin gene-related peptide and amylin in rats

1996 ◽  
Vol 314 (1-2) ◽  
pp. 115-121 ◽  
Author(s):  
William Vine ◽  
Kevin Beaumont ◽  
Bronislava Gedulin ◽  
Richard Pittner ◽  
Candace X. Moore ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

Gene transfer of calcitonin gene-related peptide to cerebral arteries

2000 ◽  
Vol 278 (2) ◽  
pp. H586-H594 ◽  
Author(s):  
Kazunori Toyoda ◽  
Frank M. Faraci ◽  
Andrew F. Russo ◽  
Beverly L. Davidson ◽  
Donald D. Heistad
Keyword(s):  
Cerebrospinal Fluid ◽  
Gene Transfer ◽  
Basilar Artery ◽  
Cultured Cells ◽  
Cerebral Arteries ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

Overexpression of calcitonin gene-related peptide (CGRP), an extremely potent vasodilator, to blood vessels is a possible strategy for prevention of vasospasm. We constructed an adenoviral vector that encodes prepro-CGRP (Adprepro-CGRP) and examined the effects of gene transfer on cultured cells and cerebral arteries. Transfection of Adprepro-CGRP to Cos-7 and NIH-3T3 cells increased CGRP-like immunoreactivity in media and produced an increase in cAMP in recipient cells. Five days after injection of Adprepro-CGRP into the cisterna magna of rabbits, the concentration of CGRP-like immunoreactivity increased by 93-fold in cerebrospinal fluid. In basilar artery, cAMP increased by 2.3-fold after Adprepro-CGRP compared with a control adenovirus. After transfection of Adprepro-CGRP, contraction of basilar artery in vitro to histamine and serotonin was attenuated, and relaxation to an inhibitor of cyclic nucleotide phosphodiesterase 3-isobutyl-1-methylxanthine was augmented compared with nontransduced arteries or arteries transfected with a control gene. Altered vascular responses were restored to normal by pretreatment with a CGRP1 receptor antagonist CGRP-(8–37). Thus gene transfer of prepro-CGRP in vivo overexpresses CGRP in cerebrospinal fluid and perivascular tissues and modulates vascular tone. We speculate that this approach may be useful in prevention of vasospasm after subarachnoid hemorrhage.

Presence and Function of the Calcitonin Gene-Related Peptide Receptor on Rat Pial Arteries Investigated In Vitro and In Vivo

Cephalalgia ◽  
2005 ◽  
Vol 25 (6) ◽  
pp. 424-432 ◽  
Author(s):  
KA Petersen ◽  
E Nilsson ◽  
J Olesen ◽  
L Edvinsson
Keyword(s):  
Cerebral Arteries ◽  
Vessel Diameter ◽  
Calcitonin Gene Related Peptide ◽  
Pial Arteries ◽  
Cranial Window ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
And Function

Calcitonin gene-related peptide (CGRP) and related peptides may be involved in migraine pathogenesis. To understand their vasomotor role in the cerebral circulation, we performed two studies, a pressurized arteriography study of the middle cerebral artery (MCA) and a genuine closed cranial window (gCCW) in vivo study. Using the pressurized arteriography model rat MCAs were mounted on micropipettes, pressurized to 85 mmHg and luminally perfused. The diameter responses to luminally and abluminally applied rat-αCGRP, rat-βCGRP, amylin and adrenomedullin were compared with the resting diameter. Only abluminally applied CGRP induced dilation of the cerebral arteries; Emax for αCGRP and βCGRP were 35 ± 0.5% and 10.8 ± 0.2%. These responses were blocked by CGRP8-37. The gCCW model allowed videomicroscopic visualization of the pial vessels in anaesthetized rats. Changes in vessel diameter to intravenously administered αCGRP and βCGRP were compared with pre-infusion baseline. Intravenous infusion of αCGRP and βCGRP in the highest dose induced dilation of the cerebral cortical pial arteries/arterioles of 40.3 ± 7.5% and 49.1 ± 8.4%, respectively. However, this was probably secondary to a decrease in blood pressure of 44.8 ± 3.3 mmHg and 49.2 ± 3.3 mmHg. Our results suggest that CGRP receptors are probably functional on the smooth muscle cells and not on the endothelium of rat cerebral arteries.

Induction of insulin resistance in vivo by amylin and calcitonin gene-related peptide

Diabetes ◽  
1990 ◽  
Vol 39 (2) ◽  
pp. 260-265 ◽  
Author(s):  
J. M. Molina ◽  
G. J. Cooper ◽  
B. Leighton ◽  
J. M. Olefsky
Keyword(s):  
Insulin Resistance ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

Tapentadol inhibits calcitonin gene-related peptide release from rat brainstem in vitro

Peptides ◽  
2014 ◽  
Vol 56 ◽  
pp. 8-13 ◽  
Author(s):  
Maria Cristina Greco ◽  
Lucia Lisi ◽  
Diego Currò ◽  
Pierluigi Navarra ◽  
Giuseppe Tringali
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Peptide Release ◽  
Rat Brainstem
Sign in / Sign up

Export Citation Format

Share Document