Molecular epidemiology of Pseudomonas Aeruginosa

2002 ◽  
Vol 7 (1-3) ◽  
pp. e354 ◽  
Author(s):  
David, P. Speert
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology

scholarly journals 132 Molecular epidemiology and dynamics of chronic Pseudomonas aeruginosa populations in Cystic Fibrosis lungs

2006 ◽  
Vol 5 ◽  
pp. S30
Author(s):  
L. Jelsbak ◽  
A. Frost ◽  
H.K. Johansen ◽  
L. Thomsen ◽  
L. Yang ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology

scholarly journals Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e17064 ◽  
Author(s):  
Simone Lanini ◽  
Silvia D'Arezzo ◽  
Vincenzo Puro ◽  
Lorena Martini ◽  
Francesco Imperi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Hospital Outbreak

Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas aeruginosa isolates from Chinese hospitals

2010 ◽  
Vol 35 (5) ◽  
pp. 486-491 ◽  
Author(s):  
Jie Wang ◽  
Jian-ying Zhou ◽  
Ting-ting Qu ◽  
Ping Shen ◽  
Ze-qing Wei ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Carbapenem Resistance

Molecular epidemiology of Pseudomonas Aeruginosa

10.2741/a929 ◽  
2002 ◽  
Vol 7 (5) ◽  
pp. e354-361 ◽  
Author(s):  
David P Speert
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology

Molecular epidemiology and carbapenem resistance of Pseudomonas aeruginosa isolated from patients with burns

2021 ◽  
Vol 30 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Younes Khalili ◽  
Mohammad Yousef Memar ◽  
Safar Farajnia ◽  
Khosro Adibkia ◽  
Hossein Samadi Kafil ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Real Time ◽  
Real Time Pcr ◽  
Random Amplified Polymorphic Dna ◽  
Carbapenem Resistance ◽  
Efflux Pumps ◽  
Common Mechanism ◽  
Care Hospital ◽  
Burn Care

Objective: The aim of this study was to investigate the molecular epidemiology and carbapenem resistance mechanisms of Pseudomonas aeruginosa isolated from patients with burns in Azerbaijan, Iran. Method: Pseudomonas aeruginosa was isolated from 38 patients with burns. Disk diffusion and agar dilution methods were used to determine antibiotic susceptibility patterns. The overproduction of AmpC β-lactamase and efflux pumps were detected by phenotypic methods. The presence of carbapenemase-encoding genes was detected by multiplex polymerase chain reaction (PCR). Expression of the OprD gene and MexAB efflux pumps were also evaluated with real-time PCR. Random amplified polymorphic DNA typing (RAPD-PCR) was used for genotyping of carbapenem-resistant Pseudomonas aeruginosa (CRPA). Results: Minimum inhibitory concentration (MIC) assays demonstrated high levels of resistance to all classes of antibiotics except colistin and polymyxin B. The initial screening by carbapenem disks indicated 24 isolates (63.15%) as CRPA. Different mechanisms of carbapenem resistance were observed, including carbapenemase production (8.4%), overexpression of AmpC (25%) and decreased expression of OprD (75%). The overexpression of MexAB efflux pumps was detected in 19 (79.1%) isolates by phenotypic assay or real-time PCR. The resistance to carbapenem was multifactorial in most cases (58.3%). The RAPD genotyping revealed different patterns with nine clusters. Conclusion: According to our results, the prevalence of CRPA is at an alarming level. Our results did not demonstrate an epidemic clone. The most common mechanism of carbapenem resistance was decreased expression of OprD. Therefore, we suggest a reconsideration in the management of CRPA infections of patients in our burn care hospital in Azerbaijan, Iran.

Molecular epidemiology of outbreaks and containment of drug-resistant Pseudomonas aeruginosa in a Tokyo hospital

2007 ◽  
Vol 13 (6) ◽  
pp. 418-422 ◽  
Author(s):  
Jun-ichiro Sekiguchi ◽  
Katsuji Teruya ◽  
Kurii Horii ◽  
Emi Kuroda ◽  
Hisami Konosaki ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Drug Resistant

scholarly journals Molecular Epidemiology of Multi-Drug Resistant Pseudomonas aeruginosa Isolates from Hospitalized Patients in Greece

2020 ◽  
Vol 8 (11) ◽  
pp. 1652
Author(s):  
Olga Pappa ◽  
Anastasia Maria Kefala ◽  
Kyriaki Tryfinopoulou ◽  
Marios Dimitriou ◽  
Kostas Kostoulas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Financial Resources ◽  
Drug Resistant ◽  
Hospital Acquired Infections ◽  
Antimicrobial Sensitivity ◽  
Tertiary Hospitals ◽  
Hospital Acquired ◽  
First Time ◽  
Hospital Transmission

Resistant Pseudomonas aeruginosa isolates are one of the major causes of both hospital-acquired infections (HAIs) and community-acquired infections (CAIs). However, management of P. aeruginosa infections is difficult as the bacterium is inherently resistant to many antibiotics. In this study, a collection of 75 P. aeruginosa clinical isolates from two tertiary hospitals from Athens and Alexnadroupolis in Greece was studied to assess antimicrobial sensitivity and molecular epidemiology. All P. aeruginosa isolates were tested for susceptibility to 11 commonly used antibiotics, and the newly introduced Double Locus Sequence Typing (DLST) scheme was implemented to elucidate the predominant clones. The tested P. aeruginosa isolates presented various resistant phenotypes, with Verona Integron-Mediated Metallo-β-lactamase (VIM-2) mechanisms being the majority, and a new phenotype, FEPR-CAZS, being reported for the first time in Greek isolates. DLST revealed two predominant types, 32-39 and 8-37, and provided evidence for intra-hospital transmission of the 32-39 clone in one of the hospitals. The results indicate that DLST can be a valuable tool when local outbreaks demand immediate tracking investigation with limited time and financial resources.

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