Zebrafish embryo extract counteracts human stem cell senescence

10.2741/s528 ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 89-104 ◽  
Author(s):  
Carlo Ventura
Keyword(s):  
Stem Cell ◽  
Zebrafish Embryo ◽  
Cell Senescence ◽  
Human Stem Cell ◽  
Embryo Extract

scholarly journals Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells

2019 ◽  
Vol 20 (11) ◽  
pp. 2646 ◽  
Author(s):  
Federica Facchin ◽  
Francesco Alviano ◽  
Silvia Canaider ◽  
Eva Bianconi ◽  
Martina Rossi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Zebrafish Embryo ◽  
Human Mesenchymal Stem Cells ◽  
Cell Senescence ◽  
Embryo Extract ◽  
Early Developmental

Stem cells undergo senescence both in vivo, contributing to the progressive decline in self-healing mechanisms, and in vitro during prolonged expansion. Here, we show that an early developmental zebrafish embryo extract (ZF1) could act as a modulator of senescence in human mesenchymal stem cells (hMSCs) isolated from both adult tissues, including adipose tissue (hASCs), bone marrow (hBM-MSCs), dental pulp (hDP-MSCs), and a perinatal tissue such as the Wharton’s Jelly (hWJ-MSCs). In all the investigated hMSCs, ZF1 decreased senescence-associated β-galactosidase (SA β-gal) activity and enhanced the transcription of TERT, encoding the catalytic telomerase core. In addition, it was associated, only in hASCs, with a transcriptional induction of BMI1, a pleiotropic repressor of senescence. In hBM-MSCs, hDP-MSCs, and hWJ-MSCs, TERT over-expression was concomitant with a down-regulation of two repressors of TERT, TP53 (p53), and CDKN1A (p21). Furthermore, ZF1 increased the natural ability of hASCs to perform adipogenesis. These results indicate the chance of using ZF1 to modulate stem cell senescence in a source-related manner, to be potentially used as a tool to affect stem cell senescence in vitro. In addition, its anti-senescence action could also set the basis for future in vivo approaches promoting tissue rejuvenation bypassing stem cell transplantation.

An Update on Human Stem Cell-Based Therapy in Parkinson';s Disease

2016 ◽  
Vol 11 (7) ◽  
pp. 561-568 ◽  
Author(s):  
Clara González ◽  
Sonia Bonilla ◽  
Ana Isabel Flores ◽  
Eva Cano ◽  
Isabel Liste
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Cell Based Therapy

scholarly journals A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells

2019 ◽  
Vol 97 ◽  
pp. 272-280 ◽  
Author(s):  
Punn Augsornworawat ◽  
Leonardo Velazco-Cruz ◽  
Jiwon Song ◽  
Jeffrey R. Millman
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Islet Cells ◽  
Three Dimensional ◽  
Human Stem Cell
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