Non-coding RNAs and embryo implantation

Zeng-Ming Yang

doi:10.2741/e313

Non-Coding RNAs in Endometrial Physiopathology

International Journal of Molecular Sciences ◽

10.3390/ijms19072120 ◽

2018 ◽

Vol 19 (7) ◽

pp. 2120 ◽

Cited By ~ 30

Author(s):

Alessandro La Ferlita ◽

Rosalia Battaglia ◽

Francesca Andronico ◽

Salvatore Caruso ◽

Antonio Cianci ◽

...

Keyword(s):

Endometrial Cancer ◽

Human Genome ◽

Human Genome Project ◽

Embryo Implantation ◽

Genome Project ◽

Rna Molecules ◽

Cellular Processes ◽

Non Coding Rnas ◽

The Human Genome Project ◽

Heterogeneous Class

The Human Genome Project led to the discovery that about 80% of our DNA is transcribed in RNA molecules. Only 2% of the human genome is translated into proteins, the rest mostly produces molecules called non-coding RNAs, which are a heterogeneous class of RNAs involved in different steps of gene regulation. They have been classified, according to their length, into small non-coding RNAs and long non-coding RNAs, or to their function, into housekeeping non-coding RNAs and regulatory non-coding RNAs. Their involvement has been widely demonstrated in all cellular processes, as well as their dysregulation in human pathologies. In this review, we discuss the function of non-coding RNAs in endometrial physiology, analysing their involvement in embryo implantation. Moreover, we explore their role in endometrial pathologies such as endometrial cancer, endometriosis and chronic endometritis.

Download Full-text

The mRNA and lncRNA landscape of the non-pregnant endometrium during the oestrus cycle in dairy goat

Animal Production Science ◽

10.1071/an18426 ◽

2019 ◽

Vol 59 (10) ◽

pp. 1803 ◽

Cited By ~ 2

Author(s):

Xiaorui Liu ◽

Lei Zhang ◽

Jiuzeng Cui ◽

Sicheng Che ◽

Yuexia Liu ◽

...

Keyword(s):

Target Genes ◽

Embryo Implantation ◽

Differentially Expressed ◽

Dairy Goat ◽

Dairy Goats ◽

Global Trends ◽

Oestrus Cycle ◽

Complex Process ◽

Non Coding Rnas ◽

Cyclic Changes

Cyclic changes in the endometrium are essential for embryo implantation in mammals; many studies report that such changes constitute a complex process involving numerous molecular mediators. In the present study, goat endometria at oestrus Day 5 and oestrus Day 15 were selected to systematically analyse the transcriptome using strand-specific Ribo-Zero RNA sequencing. Over 120 million high-quality paired-end reads were generated and 440400 transcripts were identified in the endometrial tissue of dairy goats. In total, 489 differentially expressed mRNAs and 854 differentially expressed long non-coding RNAs were identified when comparing the endometrium at goat endometria at oestrus Day 5 and oestrus Day 15. Neurotensin was found to play a potentially important role in the non-pregnant goat endometrium during the oestrus cycle. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses of the cis-target genes of the differentially expressed long non-coding RNAs showed that GO:0005198 (structural molecule activity) and ko04510 (focal adhesion) might be involved in cyclic endometrial changes. Taken together, the resulting transcriptomic profiles elucidate global trends in mRNA and lncRNA expression in non-pregnant endometria during the oestrus cycle in dairy goats.

Download Full-text

Transcriptomic Analysis of Human Endometrium Stromal Cells During Early Embryo Implantation (Within 48 Hour) in Vitro

10.21203/rs.3.rs-99005/v1 ◽

2020 ◽

Author(s):

Shuo Han ◽

Minghui Liu ◽

Shan Liu ◽

Yuan Li

Keyword(s):

Stromal Cells ◽

Embryo Implantation ◽

Regulation Of Gene Expression ◽

Early Embryo ◽

Endometrial Stromal Cells ◽

Non Coding Rnas ◽

Essential Prerequisite ◽

Key Pathways ◽

Post Implantation

Abstract BackgroundSuccessful embryo implantation is an essential prerequisite for pregnancy. Previous studies have shown that DNA methylation, histone, chromatin structure, and non-coding RNAs, microRNAs may participate in the regulation of gene expression during embryo implantation. However, the transcriptome changes of human endometrial stromal cells during early embryo implantation are not well characterized. MethodsWe cultured human endometrial stromal cells and simulated the process of embryo implantation in vitro. We further analyzed the endometrial transcriptome patterns of endometrial stromal cells in the pre-implantation and post-implantation phase. We identified comprehensive transcriptomic profile of two endometrium stromal cells in particular developmental stage that may reflect the potential mechanism of embryo implantation. ResultsA total of 592 differentially expressed genes were identified after embryo implantation. Additionally, we identified key pathways (including TP53 and EGF signal pathway) that may regulate embryo-endometrium interactions; our findings may serve as a foundation for targeted studies on endometrial receptivity and embryo implantation loss. ConclusionOur work showed the transcriptome changes of endometrial stromal cells within 48 hours after implantation which provides key insights into the crucial features of transcriptional regulation in the stepwise embryo development.

Download Full-text