Spheroids of stem cells as endochondral templates for improved bone engineering

10.2741/4683 ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 1969-1986 ◽  
Author(s):  
Leandra Santos Baptista
Keyword(s):  
Stem Cells ◽  
Bone Engineering

Feasibility of Defect Tunable Bone Engineering Using Electroblown Bioactive Fibrous Scaffolds with Dental Stem Cells

2018 ◽  
Vol 4 (3) ◽  
pp. 1019-1028 ◽  
Author(s):  
Nandin Mandakhbayar ◽  
Ahmed El-Fiqi ◽  
Khandmaa Dashnyam ◽  
Hae-Won Kim
Keyword(s):  
Stem Cells ◽  
Dental Stem Cells ◽  
Bone Engineering ◽  
Fibrous Scaffolds

Coculture of Vascular Endothelial Cells and Adipose-Derived Stem Cells as a Source for Bone Engineering

2012 ◽  
Vol 69 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Xian Zhao ◽  
Liu Liu ◽  
Fu-Ke Wang ◽  
De-Pin Zhao ◽  
Xiao-Ming Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Adipose Derived Stem Cells ◽  
Bone Engineering

scholarly journals Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1154
Author(s):  
Raúl Rosales-Ibáñez ◽  
Nieves Cubo-Mateo ◽  
Amairany Rodríguez-Navarrete ◽  
Arely M. González-González ◽  
Tomás E. Villamar-Duque ◽  
...  
Keyword(s):  
Stem Cells ◽  
3D Printing ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Axial Direction ◽  
Dental Pulp Stem Cells ◽  
Bone Engineering ◽  
Lateral Direction ◽  
Alizarin Red

The search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. A 100% pure Mw: 84,500 ± 1000 PCL was selected. 5 × 10 × 5 mm3 parallelepiped scaffolds were designed as a wood-pilled structure composed of 20 layers of 250 μm in height, in a non-alternate order ([0,0,0,90,90,90°]). 3D printing was made at 170 °C. Swine dental pulp stem cells (DPSCs) were extracted from lower lateral incisors of swine and cultivated until the cells reached 80% confluence. The third passage was used for seeding on the scaffolds. Phenotype of cells was determined by flow Cytometry. Live and dead, Alamar blue™, von Kossa and alizarin red staining assays were performed. Scaffolds with 290 + 30 μm strand diameter, 938 ± 80 μm pores in the axial direction and 689 ± 13 μm pores in the lateral direction were manufactured. Together, cell viability tests, von Kossa and Alizarin red staining indicate the ability of the printed scaffolds to support DPSCs attachment, proliferation and enable differentiation followed by mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be though to be used for further modelling and preclinical experiments in bone engineering studies.

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

Repair of deep skin burn defects with cultured epidermal stem cells and acellular amniotic membranes

2010 ◽  
Vol 34 (8) ◽  
pp. S39-S39
Author(s):  
Dewu Liu ◽  
Honglan Xiong ◽  
Yuangui Mao ◽  
Peixin Huang ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epidermal Stem Cells ◽  
Skin Burn ◽  
Amniotic Membranes
Sign in / Sign up

Export Citation Format

Share Document