Transgenic expression of human INS gene in Ins1/Ins2 double knockout mice leads to insulin underproduction and diabetes in some male mice

10.2741/2171 ◽  
2007 ◽  
Vol 12 (1) ◽  
pp. 1586 ◽  
Author(s):  
Melis Karaca
Keyword(s):  
Knockout Mice ◽  
Male Mice ◽  
Double Knockout ◽  
Transgenic Expression

3-Deazaadenosine Inhibits Vasa Vasorum Neovascularization in Aortas of ApoE-/-/LDL-/- Double Knockout Mice

2008 ◽  
Vol 180 (S 1) ◽  
Author(s):  
AC Langheinrich ◽  
D Sedding ◽  
M Kampschulte ◽  
J Wilhelm ◽  
W Haberbosch ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Vasa Vasorum ◽  
Double Knockout

scholarly journals Increase in SGLT1-mediated transport explains renal glucose reabsorption during genetic and pharmacological SGLT2 inhibition in euglycemia

2014 ◽  
Vol 306 (2) ◽  
pp. F188-F193 ◽  
Author(s):  
Timo Rieg ◽  
Takahiro Masuda ◽  
Maria Gerasimova ◽  
Eric Mayoux ◽  
Kenneth Platt ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Plasma Concentrations ◽  
Sglt2 Inhibitor ◽  
Double Knockout ◽  
Glucose Reabsorption ◽  
Urinary Glucose ◽  
Sglt2 Inhibition ◽  
Renal Glucose Reabsorption ◽  
Glucose Excretion ◽  
Free Plasma

In the kidney, the sodium-glucose cotransporters SGLT2 and SGLT1 are thought to account for >90 and ∼3% of fractional glucose reabsorption (FGR), respectively. However, euglycemic humans treated with an SGLT2 inhibitor maintain an FGR of 40–50%, mimicking values in Sglt2 knockout mice. Here, we show that oral gavage with a selective SGLT2 inhibitor (SGLT2-I) dose dependently increased urinary glucose excretion (UGE) in wild-type (WT) mice. The dose-response curve was shifted leftward and the maximum response doubled in Sglt1 knockout (Sglt1−/−) mice. Treatment in diet with the SGLT2-I for 3 wk maintained 1.5- to 2-fold higher urine glucose/creatinine ratios in Sglt1−/− vs. WT mice, associated with a temporarily greater reduction in blood glucose in Sglt1−/− vs. WT after 24 h (−33 vs. −11%). Subsequent inulin clearance studies under anesthesia revealed free plasma concentrations of the SGLT2-I (corresponding to early proximal concentration) close to the reported IC50 for SGLT2 in mice, which were associated with FGR of 64 ± 2% in WT and 17 ± 2% in Sglt1−/−. Additional intraperitoneal application of the SGLT2-I (maximum effective dose in metabolic cages) increased free plasma concentrations ∼10-fold and reduced FGR to 44 ± 3% in WT and to −1 ± 3% in Sglt1−/−. The absence of renal glucose reabsorption was confirmed in male and female Sglt1/Sglt2 double knockout mice. In conclusion, SGLT2 and SGLT1 account for renal glucose reabsorption in euglycemia, with 97 and 3% being reabsorbed by SGLT2 and SGLT1, respectively. When SGLT2 is fully inhibited by SGLT2-I, the increase in SGLT1-mediated glucose reabsorption explains why only 50–60% of filtered glucose is excreted.

P2.55 Mstn/Dysf double knockout mice gain muscle mass but no strength

2011 ◽  
Vol 21 (9-10) ◽  
pp. 676-677
Author(s):  
V. Schoewel ◽  
S. Adams ◽  
C. Herrmann ◽  
U. Zacharias ◽  
M. Boschmann ◽  
...  
Keyword(s):  
Muscle Mass ◽  
Knockout Mice ◽  
Double Knockout

scholarly journals Integrin α1/Akita double-knockout mice on a Balb/c background develop advanced features of human diabetic nephropathy

2012 ◽  
Vol 81 (11) ◽  
pp. 1086-1097 ◽  
Author(s):  
Ling Yu ◽  
Yan Su ◽  
Paisit Paueksakon ◽  
Huifang Cheng ◽  
Xiwu Chen ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Knockout Mice ◽  
Double Knockout

scholarly journals Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

2004 ◽  
Vol 164 (6) ◽  
pp. 2279-2288 ◽  
Author(s):  
Derek Paisley ◽  
Stephen Banks ◽  
Jim Selfridge ◽  
Neil F. McLennan ◽  
Ann-Marie Ritchie ◽  
...  
Keyword(s):  
Dna Damage ◽  
Male Infertility ◽  
Prion Protein ◽  
Knockout Mice ◽  
Double Knockout

Lutein and zeaxanthin reduce A2E and iso-A2E levels and improve visual performance in Abca4/bco2 double knockout mice

2021 ◽  
pp. 108680
Author(s):  
Ranganathan Arunkumar ◽  
Aruna Gorusupudi ◽  
Binxing Li ◽  
J. David Blount ◽  
Uzoamaka Nwagbo ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Visual Performance ◽  
Double Knockout

3-Deazaadenosine inhibits vasa vasorum neovascularization in aortas of ApoE−/−/LDL−/− double knockout mice

2009 ◽  
Vol 202 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Alexander C. Langheinrich ◽  
Daniel G. Sedding ◽  
Marian Kampschulte ◽  
Regina Moritz ◽  
Jochen Wilhelm ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Vasa Vasorum ◽  
Double Knockout
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