Effect of pH-variation on insertion and ion channel formation of human calcitonin into planar lipid bilayers

10.2741/1945 ◽  
2006 ◽  
Vol 11 (1) ◽  
pp. 2035 ◽  
Author(s):  
Silvia Micelli
Keyword(s):  
Ion Channel ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Human Calcitonin ◽  
Channel Formation ◽  
Effect Of Ph ◽  
Ph Variation

Effect of Sterol Side Chain on Ion Channel Formation by Amphotericin B in Lipid Bilayers

Biochemistry ◽  
2014 ◽  
Vol 53 (19) ◽  
pp. 3088-3094 ◽  
Author(s):  
Yasuo Nakagawa ◽  
Yuichi Umegawa ◽  
Tetsuro Takano ◽  
Hiroshi Tsuchikawa ◽  
Nobuaki Matsumori ◽  
...  
Keyword(s):  
Ion Channel ◽  
Amphotericin B ◽  
Lipid Bilayers ◽  
Side Chain ◽  
Channel Formation

Channel Formation in Planar Lipid Bilayers by a Neurotoxic Fragment of the β-Amyloid Peptide

1994 ◽  
Vol 202 (2) ◽  
pp. 1142-1148 ◽  
Author(s):  
T. Mirzabekov ◽  
M.C. Lin ◽  
W.L. Yuan ◽  
P.J. Marshall ◽  
M. Carman ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Amyloid Peptide ◽  
Planar Lipid Bilayers ◽  
Channel Formation ◽  
Β Amyloid ◽  
Β Amyloid Peptide

scholarly journals A consensus segment in the M2 domain of the hP2X7 receptor shows ion channel activity in planar lipid bilayers and in biological membranes

2012 ◽  
Vol 1818 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Cristina Alves Magalhães de Souza ◽  
Pedro Celso Nogueira Teixeira ◽  
Robson Xavier Faria ◽  
Oxana Krylova ◽  
Peter Pohl ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Bilayers ◽  
Biological Membranes ◽  
Planar Lipid Bilayers ◽  
Channel Activity

scholarly journals Cation channel conductance and pH gating of the innate immunity factor APOL1 are governed by pore-lining residues within the C-terminal domain

2020 ◽  
Vol 295 (38) ◽  
pp. 13138-13149 ◽  
Author(s):  
Charles Schaub ◽  
Joseph Verdi ◽  
Penny Lee ◽  
Nada Terra ◽  
Gina Limon ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Plasma Membrane ◽  
Lipid Bilayers ◽  
Cation Channel ◽  
Planar Lipid Bilayers ◽  
Channel Formation ◽  
Cation Selectivity ◽  
Terminal Domain ◽  
Immunity Factor ◽  
Pore Lining

The human innate immunity factor apolipoprotein L-I (APOL1) protects against infection by several protozoan parasites, including Trypanosoma brucei brucei. Endocytosis and acidification of high-density lipoprotein–associated APOL1 in trypanosome endosomes leads to eventual lysis of the parasite due to increased plasma membrane cation permeability, followed by colloid-osmotic swelling. It was previously shown that recombinant APOL1 inserts into planar lipid bilayers at acidic pH to form pH-gated nonselective cation channels that are opened upon pH neutralization. This corresponds to the pH changes encountered during endocytic recycling, suggesting APOL1 forms a cytotoxic cation channel in the parasite plasma membrane. Currently, the mechanism and domains required for channel formation have yet to be elucidated, although a predicted helix-loop-helix (H-L-H) was suggested to form pores by virtue of its similarity to bacterial pore-forming colicins. Here, we compare recombinant human and baboon APOL1 orthologs, along with interspecies chimeras and individual amino acid substitutions, to identify regions required for channel formation and pH gating in planar lipid bilayers. We found that whereas neutralization of glutamates within the H-L-H may be important for pH-dependent channel formation, there was no evidence of H-L-H involvement in either pH gating or ion selectivity. In contrast, we found two residues in the C-terminal domain, tyrosine 351 and glutamate 355, that influence pH gating properties, as well as a single residue, aspartate 348, that determines both cation selectivity and pH gating. These data point to the predicted transmembrane region closest to the APOL1 C terminus as the pore-lining segment of this novel channel-forming protein.

Interaction of acidic amphiphilic α-helical peptides with lipid bilayers and their ion channel formation

1993 ◽  
pp. 681-684
Author(s):  
Taichi Tanaka ◽  
Kenji Matsumoto ◽  
Sannamu Lee ◽  
Gohsuke Sugihara ◽  
Haruhiko Aoyagi ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Bilayers ◽  
Channel Formation ◽  
Helical Peptides

scholarly journals Planar lipid bilayers in recombinant ion channel research

Methods ◽  
2018 ◽  
Vol 147 ◽  
pp. 206-212 ◽  
Author(s):  
Jacqueline Maher ◽  
Marcus Allen
Keyword(s):  
Ion Channel ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers

scholarly journals Reported channel formation by prion protein fragment 106-126 in planar lipid bilayers cannot be reproduced

FEBS Letters ◽  
2000 ◽  
Vol 474 (2-3) ◽  
pp. 255-256 ◽  
Author(s):  
Maria Manunta ◽  
Beat Kunz ◽  
Erika Sandmeier ◽  
Philipp Christen ◽  
Hansgeorg Schindler
Keyword(s):  
Prion Protein ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Channel Formation ◽  
Protein Fragment
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