scholarly journals Quantifying cell-adhesion strength with micropipette manipulation: principle and application

10.2741/1386 ◽  
2004 ◽  
Vol 9 (1-3) ◽  
pp. 2183 ◽  
Author(s):  
Jin-Yu Shao
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Micropipette Manipulation

Characterization of the Effect of Geometry on Single Cell Adhesion Strength Using a Microfluidic Device

2007 ◽  
Author(s):  
Kevin V. Christ ◽  
Kyle B. Williamson ◽  
Kristyn S. Masters ◽  
Kevin T. Turner
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Adhesion Strength ◽  
Single Cells ◽  
Microfluidic Channel ◽  
Reynolds Numbers ◽  
Microfluidic Channels ◽  
Low Reynolds Numbers ◽  
Physiological Processes ◽  
Micropipette Manipulation

Cell adhesion plays a crucial role in a number of fundamental physiological processes and is important in the development of implantable biomaterials. Cell adhesion strength has previously been measured using a range of techniques, including population assays (e.g., centrifugation [1], hydrodynamic flow [2]) and single-cell methods (e.g., AFM [3], micropipette manipulation [4]). Population studies are unable to provide detailed information about individual cell behavior, while the single-cell methods are often time-consuming and difficult to perform. Microfluidic channels present a way to generate well-defined stress fields on cells [5]. The small dimensions of these channels result in low Reynolds numbers that allow for the generation of sufficiently large stresses to detach well-spread cells under laminar flow conditions. In the present work, a microfluidic channel was used to controllably load adhered single-cells to detachment and measure the adhesion strength. Using this assay, the effect of cell geometry on adhesion strength was investigated.

Hydrodynamically-Confined Microflows for Cell Adhesion Strength Measurement

2010 ◽  
Author(s):  
Kevin V. Christ ◽  
Kevin T. Turner
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Cell Behavior ◽  
Shear Stresses ◽  
Strength Measurement ◽  
Coated Surfaces ◽  
Biology Research ◽  
Standard Techniques

Cell adhesion plays a fundamental role in numerous physiological and pathological processes, and measurements of the adhesion strength are important in fields ranging from basic cell biology research to the development of implantable biomaterials. Our group and others have recently demonstrated that microfluidic devices offer advantages for characterizing the adhesion of cells to protein-coated surfaces [1,2]. Microfluidic devices offer many advantages over conventional assays, including the ability to apply high shear stresses in the laminar regime and the opportunity to directly observe cell behavior during testing. However, a key disadvantage is that such assays require cells to be cultured inside closed microchannels. Assays based on closed channels restrict the types of surfaces that can be examined and are not compatible with many standard techniques in cell biology research. Furthermore, while techniques for cell culture in microchannels have become common, maintaining the viability of certain types of cells in channels remains a challenge.

scholarly journals In-flow measurement of cell–cell adhesion using oscillatory inertial microfluidics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 1612-1620 ◽  
Author(s):  
Baris R. Mutlu ◽  
Taronish Dubash ◽  
Claudius Dietsche ◽  
Avanish Mishra ◽  
Arzu Ozbey ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Flow Measurement ◽  
Microfluidic System ◽  
Suspended Cell ◽  
Inertial Microfluidics ◽  
Cell Clusters ◽  
System P ◽  
Freely Suspended ◽  
Cell Cell

Cell–cell adhesion strength of freely suspended cell clusters can be measured using an oscillatory inertial microfluidic system.

Evaluation of human osteoblast-like cell adhesion strength on Ti substrates functionalized by bioactive peptide grafting

2006 ◽  
Vol 39 ◽  
pp. S575
Author(s):  
A. Bagno ◽  
M. Dettin ◽  
A. Piovan ◽  
P. Brun ◽  
R. Gambaretto ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Bioactive Peptide ◽  
Human Osteoblast

A Convenient and Efficient Centrifugation Assay for Comparing Cell Adhesion Strength on Uncharted Surfaces

2020 ◽  
Vol 10 (4) ◽  
pp. 462-468
Author(s):  
Xuan Zhou ◽  
Xin Zhou ◽  
Yichen Du ◽  
Xiaohua Shi ◽  
Pan You ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Population Level ◽  
Cell Types ◽  
Laboratory Equipment ◽  
Substrate Adhesion ◽  
Associated Proteins ◽  
Cell Affinity ◽  
Cell Substrate Adhesion ◽  
Different Cell Types

Regulating cell-substrate adhesion is of fundamental importance in biomaterial design and development. While an increasing number of approaches are being developed to quantify cell adhesion strength, only a fraction of these techniques provide measurements that are simple and sensitive at the living cell population level. In our previous study, the expression of adhesion-associated proteins in fibroblasts was found to change on ion-implanted silicone rubber; however, the actual effects of the modified surfaces on cellular mechanical properties remain to be probed. Here, we proposed a convenient method to compare the cell adhesion strength on various surfaces, for multiple adhesion periods and with different cell types. This method requires only common laboratory equipment. In addition, we introduced a new parameter, ECS50, which is appropriate for screening optimum centrifugal conditions when the cell affinity of the surface as a control is initially unknown. This parameter is helpful for further exploration of cell affinity on all the biomaterials of interest. The results demonstrate that this centrifugation assay is simple, efficient and adaptable in investigating the overall adhesion strength of living cells under various conditions, and therefore, it is a valid way to develop adhesion-controlled biointerface materials in the future.

scholarly journals Regulation of Cell Adhesion Strength by Peripheral Focal Adhesion Distribution

2011 ◽  
Vol 101 (12) ◽  
pp. 2903-2911 ◽  
Author(s):  
Kranthi Kumar Elineni ◽  
Nathan D. Gallant
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Focal Adhesion
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