scholarly journals DNA barcode of the Lanzones scale insect, Unaspis mabilis Lit & Barbecho (Hemiptera: Diaspididae)

2020 ◽  
Vol 14 (3) ◽  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Analyses ◽  
Dna Barcode ◽  
Scale Insect ◽  
Coi Gene ◽  
Species Identity ◽  
Management Options ◽  
Nucleotide Sequence Alignment ◽  
Coi Sequences

The mitochondrial cytochrome c oxidase subunit 1 (COI) nucleotide sequences of Unaspis mabilis Lit & Barbecho (Hemiptera: Diaspididae), are provided for the first time. The total genomic DNA of each scale insect was extracted from individuals infesting lanzones leaves from three selected sites in Los Baños, Laguna. A partial COI gene amplicon with approximately 750 bp was obtained using the primer pair PcoF1 and LepR1. Nucleotide sequence alignment showed no variation among the COI sequences from all the samples. BLASTn search yielded no significant match with any of the available sequences for Unaspis species. The closest hit was Aulacaspis tubercularis Newstead (GenBank Accession No. HM474091) with 87.4% nucleotide similarity. Nonetheless, phylogenetic analyses revealed that generated COI sequences from U. mabilis form a monophyletic clade with U. yanonensis and U. euonymi, with closer proximity to the former. These findings also strengthen the species status of U. mabilis under the genus Unaspis. The DNA barcodes generated from this study (GenBank Acc. Nos. MN114099, MN14101, and MN114102), could, therefore, be used to verify the species identity of other lanzones scale accessions, as well as monitor the distribution and spread of U. mabilis, which would greatly influence possible pest management options. KEYWORDS: cytochrome C oxidase I, COI, Lansium domesticum Correa, lanzones

scholarly journals First results of Central Asiatic Euchalcia DNA-studies: comparison of nucleotide sequence differences in COI between Euchalcia herrichi and Euchalcia gyulai (Lepidoptera: Noctuidae)

2020 ◽  
Vol 6 ◽  
pp. 1-4
Author(s):  
Stanislav K Korb
Keyword(s):  
Nucleotide Sequence ◽  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
First Results ◽  
The Difference ◽  
Coi Sequences ◽  
Lepidoptera Noctuidae ◽  
Two Populations

We submitted first results of the DNA studies of the Central Asiatic owlet moths of the genus Euchalcia. Standard cytochrome C oxidase subunit I (COI) gene fragments were sequenced for DNA barcoding of six specimens belonging to Euchalcia herrichi and Euchalcia gyulai. We compared the received sequences between discussed species and with two European Euchalcia species (E. variabilis and E. consona). We found no variability within the COI sequences of the samples collected in the same locality (Alai Mts., Kyrgyzstan), whereas the difference in COI sequences between two populations (Ketmen Mts., Kazakhstan and Alai Mts., Kyrgyzstan) was 0.005.

The use of DNA barcode evidence for inferring species of Chlorophthalmus (Aulopiformes, Chlorophthalmidae) in the Indo-West Pacific

2014 ◽  
Vol 65 (11) ◽  
pp. 1027 ◽  
Author(s):  
Martin F. Gomon ◽  
Robert D. Ward ◽  
Stephanie Chapple ◽  
Joshua M. Hale
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Morphological Evidence ◽  
Undescribed Species ◽  
West Pacific ◽  
Oxidase Subunit ◽  
Variable Nature ◽  
Gene Coi ◽  
Coi Sequences

Recent studies have revealed evidence that the identities and distributions of several Indo-West Pacific species of Chlorophthalmus, as redefined by Sato and Nakabo (2002a), are inaccurately understood and reported in the literature. The current confusion is mostly attributable to the meristic conservatism of the genus and the individually variable nature of the morphology in those species. An analysis of the DNA barcode region of cytochrome c oxidase subunit I sequences was employed to independently group specimens into natural species assemblages, providing evidence for verifying or correcting species concepts and identities. A re-examination of the morphology of vouchers in the resultant 12 groupings identified features corroborating the distinctiveness of 10 of the 12 groups at the species level. Each of the other two groups comprised two presumed species on the basis of morphological evidence that do not appear to be separable by cytochrome c oxidase subunit I gene (COI) sequences. Two undescribed species of Chloropthalmus are now known to inhabit slope waters of Australia, and a further two undescribed species were identified elsewhere.

scholarly journals First results of Central Asiatic Euchalcia DNA-studies: comparison of nucleotide sequence differences in COI between Euchalcia herrichi and Euchalcia gyulai (Lepidoptera: Noctuidae)

2020 ◽  
Vol 6 ◽  
pp. 1-4
Author(s):  
Stanislav Korb
Keyword(s):  
Nucleotide Sequence ◽  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
First Results ◽  
The Difference ◽  
Coi Sequences ◽  
Lepidoptera Noctuidae ◽  
Two Populations

We submitted first results of the DNA studies of the Central Asiatic owlet moths of the genus Euchalcia. Standard cytochrome C oxidase subunit I (COI) gene fragments were sequenced for DNA barcoding of six specimens belonging to Euchalcia herrichi and Euchalcia gyulai. We compared the received sequences between discussed species and with two European Euchalcia species (E. variabilis and E. consona). We found no variability within the COI sequences of the samples collected in the same locality (Alai Mts., Kyrgyzstan), whereas the difference in COI sequences between two populations (Ketmen Mts., Kazakhstan and Alai Mts., Kyrgyzstan) was 0.005.

Redescription of Triplectides misakianus (Matsumura 1931) (Trichoptera, Leptoceridae) in Japan with notes on its habitat

Zoosymposia ◽  
2016 ◽  
Vol 10 (1) ◽  
pp. 234-242
Author(s):  
NOBUYUKI KATSUMA ◽  
RYOICHI B. KURANISHI
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Oxidase Subunit ◽  
First Time ◽  
Mitochondrial Cytochrome C

Triplectides misakianus (Matsumura 1931) is redescribed on the basis of adults, larvae, and pupae collected in Japan. This report describes the pupal morphology of this species for the first time. A 658-bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene as a potential “DNA barcode” is provided. The habitat and conservation of the species are discussed.

scholarly journals Molecular identification and phylogenetic relationships of seven Satyrinae butterflies in Bangladesh using Cytochrome c oxidase subunit I gene

2019 ◽  
Vol 8 (1) ◽  
pp. 67-74
Author(s):  
Ananna Ghosh ◽  
Muhammad Sohel Abedin ◽  
Abdul Jabber Howlader ◽  
Md Monwar Hossain
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Identification ◽  
Phylogenetic Trees ◽  
Coi Gene ◽  
Neighbor Joining ◽  
Oxidase Subunit ◽  
Traditional Classification ◽  
Nymphalid Butterfly ◽  
Coi Sequences

The Satyrinae is a subfamily of Nymphalid butterfly, which is morphologically and ecologically the most diverse group, occurring in all habitats. In the present study, Cytochrome c oxidase subunit I (COI) gene of seven species of Satyrinae was sequenced, aligned, and used to construct phylogenetic trees. The molecular identification of these Satyrinae species was confirmed by comparing the related sequences in the National Center for Biotechnology Information (NCBI) GenBank. The base compositions of the COI sequences were 39.07% T, 16.44% C, 29.83% A, and 14.64% G, revealing a strong AT bias (68.9%). The sequence distance among Satyrinae species ranged from 0.09% to 0.18%. Phylogenetic trees were constructed by the neighbor-joining (NJ) and maximum likelihood (ML) methods, using Orthetrum sabina as an outgroup. Both trees had almost identical topologies. The sampled species in Satyrinae exhibited the following relationships: Melanitis leda + [(Mycalesis mineus+(Mycalesis gotama+Mycalesis anaxias)) + (Ypthima baldus + (Lethe chandica+Elymnias hypermnestra))], suggesting that M. leda might be distantly related with the rest of the Satyrinae species. This clustering result is almost identical to current traditional classification. This study confirms that the COI based DNA barcoding is an efficient method for the identification of butterflies including Satyrinae species and, as such, may further contribute effectively to biodiversity and evolutionary research. Jahangirnagar University J. Biol. Sci. 8(1): 67-74, 2019 (June)

A new species of sponge-inhabiting barnacle Neoacasta (Archaeobalanidae, Acastinae) in Kyushu, Japan

Crustaceana ◽  
2020 ◽  
Vol 93 (11-12) ◽  
pp. 1515-1532
Author(s):  
Meng-Chen Yu ◽  
Takefumi Yorisue ◽  
Gregory A. Kolbasov ◽  
Benny K. K. Chan
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Shell Morphology ◽  
Coi Gene ◽  
Cytochrome C Oxidase I ◽  
Morphological Examination ◽  
A New Species ◽  
I Gene

Abstract This paper describes a new species of sponge-inhabiting barnacle, Neoacasta liui sp. nov., collected in Fukuoka, Kyushu, Japan. In the present study we used both the morphological examination and DNA barcode (cytochrome c oxidase I gene) to distinguish this new species from acastine species including its congeners from Neoacasta Kolbasov, 1993. Neoacasta liui sp. nov. is similar to Neoacasta glans (Lamarck, 1818) in shell morphology, but the divergence in COI gene between them reached inter-specific differences. N. liui sp. nov. is characterized by externally latticed, narrow scutum, beak-shaped tergum with wider spur, and lack of six inwardly prominent teeth on margin of basis. N. glans having an Indo-West Pacific distribution may represent a complex of cryptic species.

scholarly journals DNA barcoding of fishes from River Song, Dehradun, Uttarakhand using mitochondrial cytochrome-c oxidase-I gene

2013 ◽  
Vol 14 (3) ◽  
pp. 113-121
Author(s):  
Madhu Thapliyal ◽  
Bipin Kumar Sati ◽  
Ravi Kumar ◽  
Tribhuwan Chandra ◽  
Ashish Thapliyal
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Fresh Water ◽  
Fish Species ◽  
Molecular Taxonomy ◽  
Dna Barcode ◽  
Fish Fauna ◽  
Coi Gene ◽  
Cytochrome C Oxidase I ◽  
The Ganges

The entire Himalayan region is well known as a global hotspot for biodiversity. Many workers have documented different aspect of biodiversity.  The Uttarakhand Himalaya region, a part of Himalayan system, is very rich in fresh water aquatic biodiversity because it has many fresh water streams and rivers within a short distance of 200 miles. All these fresh water bodies harbor diverse aquatic fauna with fishes being the most extensively studied. There are many fish species reported by many authors in Uttarakhand. Many of the fishes have similar morphological characters (morpho-metrics) and are difficult to identify. Attempts have been made to generate the DNA barcode of fishes but most of the attempt are limited to major rivers i.e. the Ganges and the Yamuna. Besides these two prominent river (the Ganges & the Yamuna), there are many supporting streams and small tributaries that are also inhabited by many species. No “long term” and “entire river scan” for fish species has ever been conducted in Uttarakhand. Our research aims to generate a molecular database (DNA Barcode) for entire fish species fauna in each small river/stream of Uttarakhand.  Using a combination of morphometric & DNA bar-coding data will provide an efficient method for species level identification and contributes considerably to taxonomic and biodiversity research. The present study is first step towards establishing molecular taxonomy database of Uttarakhand based upon the bar coding pattern of cytochrome c oxidase I (COI) gene.  We carried out an “entire river scan” of River Song (a tributary of river Ganges).  50 samples were collected from various sampling sites along the entire stretch of river and analyzed.   Data was uploaded into the BOLD database and the analysis of data using this database suggests that the genetic variability (K2P distance) distribution of 50 individuals belonging with 15 species.The average intraspecific variation using K2P and NJ methods/models was assessed. The mean intraspecific distance was less than 1.45% for 32% of species. Mean K2P distance within species, genus and family was 0.1%, 16.3% and 21.70% respectively. Our results suggest that River Song has diverse fish fauna with substantial genetic diversity.  Data from Barillius species suggests that this species has a very high variation.  There is ahighly probability that as all the rivers are scanned new data on endemic and exotic fish fauna diversity will emerge which would be helpful in conservation efforts.

scholarly journals Case of Human Infestation with Dermanyssus gallinae (Poultry Red Mite) from Swallows (Hirundinidae)

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 299
Author(s):  
Georgios Sioutas ◽  
Styliani Minoudi ◽  
Katerina Tiligada ◽  
Caterina Chliva ◽  
Alexandros Triantafyllidis ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Clinical Symptoms ◽  
Skin Lesions ◽  
Coi Gene ◽  
Dermanyssus Gallinae ◽  
Poultry Red Mite ◽  
Human Form ◽  
Bayesian Phylogenetic Analysis

Dermanyssus gallinae (the poultry red mite, PRM) is an important ectoparasite in the laying hen industry. PRM can also infest humans, causing gamasoidosis, which is manifested as skin lesions characterized by rash and itching. Recently, there has been an increase in the reported number of human infestation cases with D. gallinae, mostly associated with the proliferation of pigeons in cities where they build their nests. The human form of the disease has not been linked to swallows (Hirundinidae) before. In this report, we describe an incident of human gamasoidosis linked to a nest of swallows built on the window ledge of an apartment in the island of Kefalonia, Greece. Mites were identified as D. gallinae using morphological keys and amplifying the Cytochrome C oxidase subunit I (COI) gene by PCR. Bayesian phylogenetic analysis and median-joining network supported the identification of three PRM haplogroups and the haplotype isolated from swallows was identical to three PRM sequences isolated from hens in Portugal. The patient was treated with topical corticosteroids, while the house was sprayed with deltamethrin. After one week, the mites disappeared and clinical symptoms subsided. The current study is the first report of human gamasoidosis from PRM found in swallows’ nest.

Four new species of the primitively segmented spider genus Songthela (Mesothelae, Liphistiidae) from Chongqing Municipality, China

Zootaxa ◽  
2022 ◽  
Vol 5091 (4) ◽  
pp. 546-558
Author(s):  
ZHAOYANG CHEN ◽  
FENGXIANG LIU ◽  
DAIQIN LI ◽  
XIN XU
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Oxidase Subunit ◽  
Chongqing Municipality ◽  
Males And Females

This paper reports four new species of the primitively segmented spider genus Songthela from Chongqing Municipality, China, based on morphological characters of both males and females: S. jinyun sp. nov., S. longbao sp. nov., S. serriformis sp. nov. and S. wangerbao sp. nov. We also provide the GenBank accession codes of mitochondrial DNA barcode gene, cytochrome c oxidase subunit I (COI), for the holotype of four new species for future identification.  

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