scholarly journals Prevalence of hepatitis C virus RNA in patients with chronic renal diseases

1998 ◽  
Vol 4 (3) ◽  
pp. 548-553
Author(s):  
O. Shaker ◽  
H. Aboul Fadl ◽  
M. K. El Hatw
Keyword(s):  
Renal Failure ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Chronic Renal Failure ◽  
Obstructive Uropathy ◽  
Renal Diseases ◽  
Hcv Rna ◽  
Coding Region ◽  
Group I ◽  
Chronic Renal Diseases

The prevalence of hepatitis C virus [HCV] RNA in 80 patients with chronic renal diseases was determined. Two sets of primers from the non-coding region of the hepatitis C virus were used. The products [188 bp] amplified by polymerase chain reaction were visualized by 2% agarose gel electrophoresis stained with ethidium bromide. The patients were classified into four groups. Group I comprised 40 adult patients with end-stage renal disease, 31 of whom were positive for HCV-RNA [77.5%] ; group II, 22 children with glomerulopathies, 15 of whom were positive [68.2%] ; group III, 9 children with chronic renal failure of unverified etiology, 6 of whom were positive [66.6%] ; group IV, 9 children with chronic renal failure due to obstructive uropathy of whom 3 [33.3%] were positive. We conclude that HCV may infect a high percentage of patients with chronic renal failure or renal parenchymatous disease

Hepatitis C virus infection in patients with chronic liver disease or chronic renal failure and blood donors in Thailand

1994 ◽  
Vol 44 (3) ◽  
pp. 287-292 ◽  
Author(s):  
Pairoj Luengrojanakul ◽  
Kriengsak Vareesangthip ◽  
Termchai Chainuvati ◽  
Kazumoto Murata ◽  
Fumio Tsuda ◽  
...  
Keyword(s):  
Renal Failure ◽  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Chronic Renal Failure ◽  
Virus Infection ◽  
Chronic Liver Disease ◽  
Blood Donors ◽  
Chronic Liver ◽  
Hepatitis C Virus Infection

scholarly journals Genomic and Phylogenetic Analysis of Hepatitis C Virus Isolates from Argentine Patients: a Six-Year Retrospective Study

2000 ◽  
Vol 38 (12) ◽  
pp. 4560-4568 ◽  
Author(s):  
J. F. Quarleri ◽  
B. H. Robertson ◽  
V. L. Mathet ◽  
M. Feld ◽  
L. Espı́nola ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Fragment Length Polymorphism ◽  
Hcv Rna ◽  
Coding Region ◽  
Serum Samples ◽  
Phylogenetic Relatedness ◽  
Clade 1 ◽  
Virus Isolates ◽  
Rflp Typing

Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23.0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5′ untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.

scholarly journals Genomic-Scale Interaction Involving Complementary Sequences in the Hepatitis C Virus 5′UTR Domain IIa and the RNA-Dependent RNA Polymerase Coding Region Promotes Efficient Virus Replication

Viruses ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 17 ◽  
Author(s):  
Elodie Rance ◽  
Jerome Tanner ◽  
Caroline Alfieri
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Progeny Virus ◽  
Hcv Rna ◽  
Genomic Rna ◽  
Coding Region ◽  
Rna Dependent Rna Polymerase ◽  
High Affinity

The hepatitis C virus (HCV) genome contains structured elements thought to play important regulatory roles in viral RNA translation and replication processes. We used in vitro RNA binding assays to map interactions involving the HCV 5′UTR and distal sequences in NS5B to examine their impact on viral RNA replication. The data revealed that 5′UTR nucleotides (nt) 95–110 in the internal ribosome entry site (IRES) domain IIa and matching nt sequence 8528–8543 located in the RNA-dependent RNA polymerase coding region NS5B, form a high-affinity RNA-RNA complex in vitro. This duplex is composed of both wobble and Watson-Crick base-pairings, with the latter shown to be essential to the formation of the high-affinity duplex. HCV genomic RNA constructs containing mutations in domain IIa nt 95–110 or within the genomic RNA location comprising nt 8528–8543 displayed, on average, 5-fold less intracellular HCV RNA and 6-fold less infectious progeny virus. HCV genomic constructs containing complementary mutations for IRES domain IIa nt 95–110 and NS5B nt 8528–8543 restored intracellular HCV RNA and progeny virus titers to levels obtained for parental virus RNA. We conclude that this long-range duplex interaction between the IRES domain IIa and NS5B nt 8528–8543 is essential for optimal virus replication.

scholarly journals Characterization of Hepatitis C Virus Subgenomic Replicon Resistance to Cyclosporine In Vitro

2007 ◽  
Vol 81 (11) ◽  
pp. 5829-5840 ◽  
Author(s):  
John M. Robida ◽  
Heather B. Nelson ◽  
Zhe Liu ◽  
Hengli Tang
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cellular Protein ◽  
Live Cells ◽  
Hcv Rna ◽  
Coding Region ◽  
Subgenomic Replicon ◽  
Cyclophilin B

ABSTRACT Treatment of hepatitis C virus (HCV) infection has been met with less than satisfactory results due primarily to its resistance to and significant side effects from alpha interferon (IFN-α). New classes of safe and broadly acting treatments are urgently needed. Cyclosporine (CsA), an immunosuppressive and anti-inflammatory drug for organ transplant patients, has recently been shown to be highly effective in suppressing HCV replication through a mechanism that is distinct from the IFN pathway. Here we report the selection and characterization of HCV replicon cells that are resistant to CsA treatment in vitro, taking advantage of our ability to sort live cells that are actively replicating HCV RNA in the presence of drug treatments. This resistance is specific to CsA as the replicon cells most resistant to CsA were still sensitive to IFN-α and a polymerase inhibitor. We demonstrate that the resistant phenotype is not a result of general enhanced replication and, furthermore, that mutations in the coding region of HCV NS5B contribute to the resistance. Interestingly, a point mutation (I432V) isolated from the most resistant replicon was able to rescue a lethal mutation (P540A) in NS5B that disrupts its interaction with its cofactor, cyclophilin B (CypB), even though the I432V mutation is located outside of the reported CypB binding site (amino acids 520 to 591). Our results demonstrate that CsA exerts selective pressure on the HCV genome, leading to the emergence of resistance-conferring mutations in the viral genome despite acting upon a cellular protein.

Hepatitis C Virus Infection among Chronic Renal Failure Patients Pre – Dialysis Therapy in Hodiedah City, Yemen

2017 ◽  
Vol 18 (1) ◽  
pp. 1-6
Author(s):  
Mansoor Awfi ◽  
Mohammed Al-Kamarany ◽  
Rasheed Mohsen ◽  
Mohammed Hilali ◽  
Waleed Khader ◽  
...  
Keyword(s):  
Renal Failure ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Chronic Renal Failure ◽  
Virus Infection ◽  
Hepatitis C Virus Infection ◽  
Dialysis Therapy

Seropositivity to hepatitis C virus (HCV) in Saudi children with chronic renal failure maintained on haemodialysis

1992 ◽  
Vol 12 (2) ◽  
pp. 217-219 ◽  
Author(s):  
M. Al-Mugeiren ◽  
F. Z. Al-Faleh ◽  
S. Ramia ◽  
S. Al-Rasheed ◽  
M. A. Mahmoud ◽  
...  
Keyword(s):  
Renal Failure ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Chronic Renal Failure
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