scholarly journals Effect of oven and freeze drying on antioxidant activity, total phenolic and total flavonoid contents of fig (Ficus carica L.) leaves

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2114-2121
Author(s):  
I.M. Elshaafi ◽  
K.H. Musa ◽  
N. Abdullah Sani
Keyword(s):  
Freeze Drying ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Flavonoid ◽  
Ficus Carica ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Oven Drying ◽  
Freeze Dried

Effect of drying processes on the antioxidant activities, total phenolic (TPC) and total flavonoid (TFC) contents of three fig (Ficus carica L.) leaves’ cultivars namely Brown Turkey Masuri 6 (BTM 6), Masui Dauphine Jumbo (MD-J) and Taiwan Golden Fish Jumbo (TGF-J) were studied. Oven drying which was conducted at 40oC, 50oC, 60oC and freeze drying at -80oC were run for 48 hrs. Antioxidant activities were evaluated using radical-scavenging capacity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric-reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays. TPC and TFC were evaluated using Folin-Ciocaltue and aluminum chloride assays respectively. It was found that using oven drying at 40oC revealed significantly (p<0.05) the highest antioxidant activities of the three fig cultivars followed by oven drying at 50oC, freeze dried and oven drying at 60oC respectively. Fresh leaves revealed the significantly lowest antioxidant activities for all antioxidant assays and for all fig cultivars. Regardless of the drying process, fig cultivar BTM 6 revealed significantly (p<0.05) the highest antioxidant activities followed by TGF-J and MD-J. Positive correlations between antioxidant, TPC and TFC activities of fig leaves extracts were observed at oven drying at 40oC, oven drying at 60oC and freeze drying but not with oven drying at 50oC and fresh samples. High levels of antioxidant activities were obtained in F. carica L. leave samples (all cultivars), indicating that the leaves have potential as a source of natural antioxidants compounds.

scholarly journals Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Omidreza Firuzi ◽  
Katayoun Javidnia ◽  
Maryam Gholami ◽  
Mohammad Soltani ◽  
Ramin Miri
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

Antioxidant Activities of Mirabilis jalapa L. Seed Epicarp Extract

2012 ◽  
Vol 550-553 ◽  
pp. 1768-1772
Author(s):  
Xin Hong Wang ◽  
Jing Tao Dai
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mirabilis Jalapa

Mirabilis jalapa L. (Nyctaginaceae) is a traditional specie and widely distributed in most parts of China. Its leaves, roots, seed and flowers are used as drugs pharmacological activity. This is the first report on the biological activity against activities of scavenging free radical and ferric reducing antioxidant power (FRAP). Mirabilis jalapa L. seed epicarp (MJSE) was immersed with ethanol (or methanol, ethyl acetate, water) in the ultrasonic instrument, then determination of total phenolic content (TPC) and total flavonoid content (TFC) of these extracts were performed. Furthermore, antioxidant activities of these extracts were evaluated by using DPPH radical scavenging, hydroxyl radical scavenging and FRAP. The TPC, TFC, FRAP and scavenging activities against DPPH and OH radical were in the following order ethyl acetate >ethanol>methanol>water. The TPC of ethyl acetate extract is 4.24 mg GAE /g of dry power weight (DPW), and TFC 0.39 mg RE/ g DPW. ethyl acetate extract exhibit high free radical scavenging rate, IC50 of DPPH and OH assay were 6.62 mg DPW/ml and 3.49 mg DPW/ml, respectively. The TPC values, IC50 obtained from DPPH assay and FRAP assay were highly correlated with correlation of determination (R2 = 0.9878, R2 = 0.9419). The present study confirmed that MJSE has a strong antioxidant activity and can be considered to be safe and an inexpensive source of natural antioxidants and drugs.

scholarly journals Phytochemical and technological characterization of canistel dehydrated pulp: a new potential food ingredient

2021 ◽  
Vol 10 (1) ◽  
pp. e16410111577
Author(s):  
Fernando Antônio Anjo ◽  
Bianka Rocha Saraiva ◽  
Camilla Yara Langer Ogawa ◽  
Ana Carolina Pelaes Vital ◽  
Francielle Sato ◽  
...  
Keyword(s):  
Food Industry ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Technological Characteristic ◽  
Food Ingredient ◽  
Tropical Fruit ◽  
Antioxidant Power ◽  
Hygroscopic Properties ◽  
Freeze Dried

Canistel (Pouteria campechiana (Kunth) Baehni) is a tropical fruit with a yellow pulp due to the presence of carotenoids, which may be interesting for application by the food industry. The aim of this research was to analyze the phytochemical and technological characteristic of dehydrated canistel pulp by thermal dehydration and freeze-drying techniques. Test for antioxidant activities (DPPH and ABTS radical scavenging; ferric reducing antioxidant power - FRAP), total polyphenols, flavonoids, carotenoids content (UV-Vis, FTIR and Raman spectroscopy), color, and hygroscopic properties were performed. The freeze-dried sample showed a higher carotenoids concentration, more intense coloring and better hydration properties than the thermally dehydrated sample. Functional groups such as water, carotenoids, primary and secondary amides, aldehydes groups, phenolic acids, aromatic rings, cellulose, polysaccharides and niacin were identified by FTIR in both samples. Ingredient obtained by freeze drying showed better technological characteristics, allowing its use by the food industry.

scholarly journals Application of Heating on the Antioxidant and Antibacterial Properties of Malaysian and Australian Stingless Bee Honey

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1365
Author(s):  
Nurul Ainaa Farhanah Mat Ramlan ◽  
Aina Syahirah Md Zin ◽  
Nur Fatihah Safari ◽  
Kim Wei Chan ◽  
Norhasnida Zawawi
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Heat Treatments ◽  
Stingless Bee ◽  
Total Phenolic ◽  
Antioxidant Power

In the honey industry, heat treatments are usually applied to maintain honey’s quality and shelf life. Heat treatment is used to avoid crystallisation and allow the easy use of honey, but treatment with heat might affect the antioxidant and antibacterial activities, which are the immediate health effects of honey. This study will determine the effect of heat treatment on Malaysian and Australian stingless bee honey (SBH) produced by the common bee species in both countries. Eighteen honey samples were subjected to heat at 45 °C, 55 °C and 65 °C for one hour and subsequently analysed for their total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and minimum inhibitory concentration (MIC). The results show that all samples had high TPC, TFC and antioxidant activities before the treatment. The heat treatments did not affect (p < 0.05) the TPC, TFC and antioxidant activities in most samples, but did inhibit the antibacterial activities consistently in most of the samples, regardless of the bee species and country of origin. This study also confirms a strong correlation between TPC and TFC with FRAP activities for the non-heated and heated honey samples (p < 0.05). Other heat-sensitive bioactive compounds in SBH should be measured to control the antibacterial properties present.

scholarly journals Review on in vitro antioxidant activities of Curcuma species commonly used as herbal components in Indonesia

Food Research ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 286-293 ◽  
Author(s):  
Abdul Rohman ◽  
H. Widodo ◽  
E. Lukitaningsih ◽  
M. Rafi ◽  
Nurrulhidayah A.F. ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
In Vitro Tests ◽  
Antioxidant Power ◽  
Ferric Thiocyanate ◽  
Herbal Components

Free radicals, reactive nitrogen species (RNS) and reactive oxygen species (ROS) have been known to contribute several degenerative diseases such as cardiovascular diseases, aging, certain types of cancers, rheumatoid arthritis, neurodegenerative, and diabetes mellitus. In order to overcome the negative effects of these radicals, some scientists have explored some natural antioxidants from plants and it's by-products. The antioxidant can be defined as any substances or samples capable of inhibiting free radical reactions in the oxidation reaction. Due to curcuminoids contained, Curcuma species such as Curcuma longa, Curcuma heyneana, Curcuma mangga, and Curcuma xanthorriza were commonly used for herbal components in some traditional medicine. Several in vitro tests been introduced and used to measure antioxidant activities, namely radical scavenging assay using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6 -sulfonic acid) (ABTS•+), ferric reducing antioxidant power (FRAP), ferric-thiocyanate, phosphomolybdenum method, cupric ion reducing antioxidant capacity, metal chelating power, beta-carotene bleaching linoleic-ferric-thiocyanate, and thiobarbituric acid methods. This review highlighted the antioxidant activities in vitro of C. longa, C. heyneana, C. mangga, and C. xanthorriza through several tests. To perform this review, several repute databases were analyzed and used. From this review, it can be stated that Curcuma species have powerful antioxidant activities, therefore they could be potential sources of natural antioxidants and can be used as food supplements.

scholarly journals Extraction, Purification and In Vitro Antioxidant Activity Evaluation of Phenolic Compounds in California Olive Pomace

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 174
Author(s):  
Hefei Zhao ◽  
Roberto J. Avena-Bustillos ◽  
Selina C. Wang
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
The United States ◽  
Total Phenolic ◽  
Olive Pomace ◽  
Antioxidant Power ◽  
Practical Applications ◽  
Crude Extracts

Olive pomace (OP) is a valuable food byproduct that contains natural phenolic compounds with health benefits related to their antioxidant activities. Few investigations have been conducted on OP from the United States while many studies on European OP have been reported. OP of Arbequina, the most common cultivar from California, was collected and extracted by water, 70% methanol and 70% ethanol, followed by purification using macroporous absorbing resin. Results showed that the extractable total phenolic content (TPC) was 36–43 mg gallic acid equivalents (GAE)/g in pitted, drum-dried defatted olive pomace (DOP), with major contributions from hydroxytyrosol, oleuropein, rutin, verbascoside, 4-hydroxyphenyl acetic acid, hydroxytyrosol-glucoside and tyrosol-glucoside. Macroporous resin purification increased TPC by 4.6 times the ethanol crude extracts of DOP, while removing 37.33% total sugar. The antioxidant activities increased 3.7 times Trolox equivalents (TrE) by DPPH and 4.7 times TrE by ferric reducing antioxidant power (FRAP) in the resin purified extracts compared to the ethanol crude extracts. This study provided a new understanding of the extraction of the bioactive compounds from OP which could lead to practical applications as natural antioxidants, preservatives and antimicrobials in clean-label foods in the US.

scholarly journals Phenolic Compounds, Antioxidant Activities, and Inhibitory Effects on Digestive Enzymes of Different Cultivars of Okra (Abelmoschus esculentus)

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1276
Author(s):  
Ding-Tao Wu ◽  
Xi-Rui Nie ◽  
Dan-Dan Shen ◽  
Hong-Yi Li ◽  
Li Zhao ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Digestive Enzymes ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Inhibitory Effects ◽  
Antioxidant Power ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

In this study, the phenolic profiles and bioactivities of five representative cultivars of okra collected in China were investigated. Noticeable variations of phenolic compounds and their bioactivities were observed among these different cultivars of okra. The contents of total flavonoids (TFC) in “Shuiguo”, “Kalong 8”, “Kalong 3”, “Wufu”, and “Royal red” ranged from 1.75 to 3.39 mg RE/g DW, of which “Shuiguo” showed the highest TFC. Moreover, five individual phenolic compounds were found in okra by high performance liquid chromatography analysis, including isoquercitrin, protocatechuic acid, quercetin-3-O-gentiobioside, quercetin, and rutin, while isoquercitrin and quercetin-3-O-gentiobioside were detected as the main phenolic compounds in okra. Moreover, all tested okra exhibited significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity, 2,2’-azino-bis (3-ethylenzthiazoline-6-sulphonic acid) radical scavenging capacity, and ferric reducing antioxidant power) and inhibitory effects on digestive enzymes (lipase, α-glucosidase, and α-amylase). Indeed, “Shuiguo” exhibited much better antioxidant activities and inhibitory activities on digestive enzymes, which might be attributed to its high TFC. Results suggested that okra, especially “Shuiguo”, could be developed as natural antioxidants and inhibitors against hyperlipidemia and hyperglycemia in the fields of functional foods and pharmaceuticals, which could meet the increasing demand for high-quality okra with health-promoting properties in China.

scholarly journals Phytochemicals and In-Vitro Antioxidant Activities of Aloe Vera Gel

2020 ◽  
Vol 44 (1) ◽  
pp. 33-41
Author(s):  
Md Nazim Uddin ◽  
Subrata Chandra Roy ◽  
Abdulla All Mamun ◽  
Kanika Mitra ◽  
Md Zahurul Haque ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Aloe Vera ◽  
The Other ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Aloe Vera Gel ◽  
Gallic Acid Equivalent

The phytochemicals (total phenolic, tannin, flavonoid, alkaloid, and saponin) contents in the Aloe vera gel derived from the leaf of Aloe vera (L.) Burm. f. (Synonym Aloe barbadensismiller) were extracted and their antioxidant capacity was studied by Ferric reducing antioxidant power assay (FRAP), by free radical-scavenging capability using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Three different solvents with three different polarities CH3OH, CH3OH: HCl, CH3CH3OH: H2O were used at v/v ratio of 100, 98:2, 70:30, respectively. The acidified methanol solvent extracted the highest amounts of phytochemicals including total phenolic (4.64 mg gallic acid equivalent/g), tannin (3.84 mg tannic acid equivalent/g), alkaloid (662 mg piperine equivalent/g), and saponin (353 mg diosgenin equivalents/g) compared to the other two solvents. Similarly, in the extract with acidified methanol solvent, high level of total antioxidant activity (about 12 mg gallic acid equivalent/g) and scavenging effects expressed as 50% inhibition concentrations (IC50) for DPPH and ABTS assay were determined to be about 61 μg/mL and 371 μg/mL, respectively, which are higher than those with the other two solvents. The gel extract could be used as a potent antioxidant in medicine and food industries. Journal of Bangladesh Academy of Sciences, Vol. 44, No. 1, 33-41, 2020

scholarly journals Antioxidant Potential of Selected Korean Edible Plant Extracts

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yaejin Woo ◽  
Hyeji Lee ◽  
Yong-Seob Jeong ◽  
Ga Young Shin ◽  
Ja Gyeong Oh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Phenolic ◽  
Edible Plant ◽  
Camellia Japonica ◽  
Antioxidant Power

This study aimed to evaluate the antioxidant activity of various plant extracts. A total of 94 kinds of edible plant extracts obtained from the Korea Plant Extract Bank were screened for cytotoxicity, following which the total phenolic content of 24 shortlisted extracts was determined. Of these, extracts from three plants, namely, Castanea crenata (CC) leaf, Camellia japonica (CJ) fruit, and Viburnum dilatatum (VD) leaf, were examined for antioxidant capabilities by measuring radical scavenging activity, ferric reducing/antioxidant power, and lipid peroxidation inhibitory activity. In addition, cellular antioxidant activities of the three extracts were assessed by a cell-based dichlorofluorescein assay and antioxidant response element (ARE) reporter activity assay. The results demonstrated that all three extracts concentration-dependently scavenged free radicals, inhibited lipid peroxidation, reduced the cellular level of reactive oxygen species, and increased ARE-luciferase activity, indicating antioxidant enzyme-inducing potential. In particular, CJ extract showed significantly greater antioxidative activity and antimigratory effect in a breast cancer cell line compared to CC and VD extracts. Hence, CJ extract deserves further study for its in vivo functionality or biologically active constituents.

scholarly journals Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction

Marine Drugs ◽  
2018 ◽  
Vol 16 (8) ◽  
pp. 257 ◽  
Author(s):  
Marco Garcia-Vaquero ◽  
Gaurav Rajauria ◽  
Brijesh Tiwari ◽  
Torres Sweeney ◽  
John O’Doherty
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Laminaria Digitata ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Freeze Dried ◽  
Ultrasound Assisted

The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.

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