scholarly journals In-vitro analysis of maxillary first molars morphology using three dimensional Micro-CT imaging: considerations for restorative dentistry

2019 ◽  
pp. 75-81 ◽  
Author(s):  
Ismail Hakki Baltacioglu ◽  
◽  
Gulbike Demirel ◽  
Mehmet Eray Kolsuz ◽  
Kaan Orhan ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Ct Imaging ◽  
Micro Ct ◽  
Restorative Dentistry ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals In Vitro Analysis and In Vivo Tumor Targeting of a Humanized, Grafted Nanobody in Mice Using Pinhole SPECT/Micro-CT

2010 ◽  
Vol 51 (7) ◽  
pp. 1099-1106 ◽  
Author(s):  
I. Vaneycken ◽  
J. Govaert ◽  
C. Vincke ◽  
V. Caveliers ◽  
T. Lahoutte ◽  
...  
Keyword(s):  
Tumor Targeting ◽  
Micro Ct ◽  
Pinhole Spect ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Annika Osswald ◽  
Zhongke Sun ◽  
Verena Grimm ◽  
Grace Ampem ◽  
Karin Riegel ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Tumor Therapy ◽  
Three Dimensional ◽  
Tumor Spheroids ◽  
Gene Delivery Vectors ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals In vitro analysis of dental ceramics: evaluation of the radiopacity and chemical composition by raman spectroscopy

2018 ◽  
Vol 21 (2) ◽  
pp. 164
Author(s):  
Rodrigo Furtado De Carvalho ◽  
Fernando Luiz Goulart Cruz ◽  
Debora Pinto Antunes ◽  
Evane Gonçalves Toledo Júnior ◽  
Luiz Fernando Cappa De Oliveira ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Composition ◽  
Analysis Of Variance ◽  
Dental Ceramics ◽  
Restorative Dentistry ◽  
Digital Radiographs ◽  
Vitro Analysis ◽  
Post Hoc ◽  
In Vitro Analysis

<p><strong>Objective</strong>: This study compared the radiopacity of different ceramic systems by means of digital radiographs and evaluate the chemical composition of the samples by Raman spectroscopy. <strong>Material and Methods</strong>: The hypothesis tested was that there was a difference in radiopacity among the tested materials. Specimens were prepared for each ceramic tested: FLD - VM7 (VITA Zahnfabrik), LD - IPS Empress e.max Press (IPS Empress), AL - In Ceram Alumina (VITA Zahnfabrik), ALYZ - In Ceram Zirconia (VITA Zahnfabrik), YZ - Lava All Ceram (3M/ESPE), and MYZ - Zirconzahn (Talladium Brazil). The specimens were radiographed and submitted to radiographic density readings using a histogram tool. The spectrometer coupled to a petrographic microscope was used for Raman spectroscopy measurements. Analysis of variance (ANOVA) and a Tukey post-hoc test were used to compare radiopacity of the different materials. <strong>Results</strong>: For all tested materials, the radiopacity showed statistically significant differences, except YZ and MYZ. Lava All Ceram and ZirkonZahn had high radiopacity values and VM7 and IPS Empress e.max Press showed lower radiopacity than human dental structures. <strong>Conclusion</strong>: It was possible to conclude that radiopacity is closely linked to ceramic chemical composition.</p><p><strong>Keywords</strong></p><p>Dental ceramics; Radiopacity; Raman spectroscopy test; Restorative dentistry.</p>

scholarly journals Correlative imaging of collagen fibers and fibroblasts using CLEM optimized for picrosirius red staining and FIB/SEM tomography

Microscopy ◽  
2020 ◽  
Vol 69 (5) ◽  
pp. 324-329
Author(s):  
Shingo Hirashima ◽  
Keisuke Ohta ◽  
Tomonoshin Kanazawa ◽  
Akinobu Togo ◽  
Risa Tsuneyoshi ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Collagen Fibers ◽  
Target Area ◽  
Architectural Analysis ◽  
Correlative Imaging ◽  
Ultrathin Sections ◽  
Vitro Analysis ◽  
Picrosirius Red ◽  
In Vitro Analysis

Abstract Conventional imaging for three-dimensional (3D) ultra-architectural analysis of collagen fibers and fibroblasts is time-consuming and requires numerous ultrathin sections to search the target area. Currently, no method allows 3D ultra-architectural analysis of predetermined areas including spatial relationships between collagen fibers and fibroblasts in vitro. Herein, we developed a new method for in vitro analysis of the 3D ultrastructure of fibroblasts and collagen fibers using CLEM optimized for picrosirius red staining and FIB/SEM tomography. Collagen fibers were observed between, rather than on top of, stacked cells. This method offers the advantage of mesoscopic and ultrastructural analysis, thus minimizing bias and ensuring accurate observation.

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