Comparative analysis of NS1 antigen card test and ELISA in clinically suspected dengue fever patients at a tertiary hospital

2021 ◽  
Vol 19 (1) ◽  
pp. 19-22
Author(s):  
Rajeshwari K G ◽  
Keyword(s):  
Comparative Analysis ◽  
Dengue Fever ◽  
Tertiary Hospital ◽  
Ns1 Antigen ◽  
Card Test

scholarly journals Clinicohaematological profile of dengue in children: a hospital based study

2017 ◽  
Vol 4 (4) ◽  
pp. 1340 ◽  
Author(s):  
Irshad Abdul Majeed ◽  
K. Shreedhara Avabratha ◽  
Lokesha R. Gowda ◽  
Sadia Syeda
Keyword(s):  
Dengue Fever ◽  
Early Recognition ◽  
Tertiary Care ◽  
Clinical Manifestations ◽  
Haematological Parameters ◽  
Severe Dengue ◽  
Clinical Feature ◽  
Igm Elisa ◽  
Ns1 Antigen ◽  
Atypical Presentations

Background: Dengue fever is one of the most common arbo virus mediated outbreaks, being reported from different parts of the world. Now as the outbreaks are hitting different geographic locations, different clinical manifestations are being reported recently. The aim of this study is to document varied clinical manifestations and haematological parameters of dengue patients in a tertiary care centre.Methods: A total 130 cases of any of NS1 antigen, IgM card test positive or IgM ELISA positive dengue patients were included in this observational study. Clinical and haematological parameters were noted and analysed statistically.Results: Most common clinical feature was fever (100%) followed by headache (51.5%). Atypical features like seizures due to encephalitis was seen in a child with dengue. Seizure were present in 1.5% of cases, two children died due to severe dengue with shock and multi organ failure. In our study 26.92% of patients had thrombocytopenia. The mean Hb was 12.86 g/dl and platelet count was 104202/mm3.Conclusions: Fever and headache are the main features of dengue. However, one should be aware of different atypical presentations of dengue fever to diagnose and intervene timely. Early recognition of complication and timely intervention are required in the management of dengue cases. 

scholarly journals A Comparative Analysis of the Relative Efficacy of Vector-Control Strategies Against Dengue Fever

2014 ◽  
Vol 76 (3) ◽  
pp. 697-717 ◽  
Author(s):  
Marcos Amaku ◽  
Francisco Antonio Bezerra Coutinho ◽  
Silvia Martorano Raimundo ◽  
Luis Fernandez Lopez ◽  
Marcelo Nascimento Burattini ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Vector Control ◽  
Dengue Fever ◽  
Control Strategies ◽  
Relative Efficacy

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals Investigation of Dengue Fever Outbreak in a Rural Area of Islamabad, Pakistan: A Case Control Study

2021 ◽  
Author(s):  
Fawad Khalid Khan ◽  
Khurram Shahzad Akram ◽  
Ambreen Chaudhry ◽  
Mir Muhammad Hassan Bullo ◽  
zakir Hussain ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Nonstructural Protein ◽  
Health Department ◽  
Control Measures ◽  
Outbreak Investigation ◽  
Blood Samples ◽  
Breeding Sites ◽  
Nonstructural Protein 1 ◽  
Breeding Grounds ◽  
Ns1 Antigen

Background: In the second week of October 2019, five suspected cases of dengue fever were reported from union council Sohan, Islamabad rural (population 45,747) to the health department, Islamabad Capital Territory (ICT).  Outbreak investigation was conducted with the objectives to identify risk factors and to recommend control measures. Methods: Outbreak investigation was conducted from 17 th October to 25 th November 2019. A case was defined as, “fever and two or more of the following signs/symptoms; headache, retro-orbital pain, joint/bone pain, myalgia and petechial rash with NS1 test (Nonstructural Protein 1) positive during 8 th October to 25 th November 2019 among residents of Sohan”. Age and sex-matched controls were recruited from the same neighborhood. All cases were positive for NS1 antigen. Blood samples from five suspected cases were collected and tested for laboratory confirmation. Results:   A total of 547 households were surveyed and 85 cases were identified. The mean age was 34.4 years + 16.05 (range 3-71 years). The attack rate was 0.19% whereas the most affected age group was the 45-54 years (AR 0.43%). Males were predominantly affected (n=48 56.5%). Among all cases, 32% (n=27)) had stagnant water inside or around their houses (aOR 2.65, CI 1.20-5.83, P= 0.005), 33% (n=28) were using mosquito repellent (aOR 0.35, CI 0.17-0.70, P <0.001), 31% (n=26) used indoor residual spray insecticide (aOR 0.48, CI 0.24-0.97, P =0.041), and 73% (n=62) used full protective clothing (aOR 0.17, CI 0.05-0.58, P <0.001). All five blood samples were tested positive for NS-1 antigen. Conclusion: The presence of accumulated rainwater in pools and empty receptacles around houses acted as breeding grounds for Aedes aegypti mosquitos and was the most probable cause of this outbreak. Following our recommendations, the health department-initiated mosquito breeding sites control activities through residual insecticide spray and advocacy on the use of protective measures against mosquito bites.

scholarly journals Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

2019 ◽  
Vol 7 (5) ◽  
pp. 1670
Author(s):  
Sarita Otta ◽  
Bichitrananda Swain
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Rapid Test ◽  
Febrile Illness ◽  
Serum Samples ◽  
Serological Tests ◽  
Ns1 Antigen ◽  
Elisa Technique ◽  
Low Platelet Count ◽  
Sensitivity Specificity

Background: Dengue fever often presents as an undifferentiated febrile illness requiring a laboratory test for identification. Serological tests particularly on rapid kits for the detection of NS1Antigen, IgG and IgM antibodies are the most commonly performed test across the country.Methods: The serum samples of suspected dengue cases were tested by Rapid test kits for assessing all the three parameters as well as by ELISA for NS1 antigen test. The platelet count of the patients was obtained from automated coulter counter. The results thus obtained were analyzed in Excel format.Results: The serum samples from 304 suspected Dengue fever cases were received in the lab, of which 190 samples were positive either by rapid or ELISA and 176 when rapid card test was considered alone Highest seropositivity of dengue cases were observed in the age group of ≥60 years (79.2%) followed by 45-59 years (70.7%). On rapid test, 78 cases were NS1 antigen positive of which 60 cases were positive only for NS1 antigen. When NS1 rapid and ELISA tests when compared, 16 kit negative tests were positive on ELISA while 34 kit positive tests were ELISA negative.  Sensitivity, specificity, PPV and NPV when only NS1 was considered on rapid test kits when compared with ELISA were 78.9%, 87.8%, 63.8% and 93.8%. 33.5% of serologically positive cases of Dengue had low platelet count on admission while only among negative cases 17.2% had a low platelet.Conclusions: Rapid kits often show variable results thus needing a validation of them from end user. As a positive dengue test result is an essential prerequisite for diagnosis thus it is essential that for serological tests ELISA technique should be used for reporting. Thus, it also mandates more efforts at decentralization of NVBDCP to include both government and non government institutions.

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