scholarly journals STANDARDIZATION OF MEDIUM FOR SYNTHETIC SEED GERMINATION OF SALVIA SCLAREA L.

2015 ◽  
Vol 2 (1) ◽  
pp. 118-120
Author(s):  
Durgha H ◽  
Ramya G ◽  
Gogul Ramanth M ◽  
Thirugnanasampandan R
Keyword(s):  
Seed Germination ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Salvia Sclarea

Young nodal explants (0.5-1cm) of Salvia sclarea L. was used for synthetic seed preparation.Synthetic seeds were prepared using 5% sodium alginate and 1.11% calcium chloride. Seed germination was observed on MS medium fortified with 1.4µM GA3+4.4µM BA after twenty days of culture. Further multiple shoot induction was observed after fifteen days of shootinduction.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

scholarly journals An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

2015 ◽  
Vol 15 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Jyothi Abraham ◽  
T. Dennis Thomas
Keyword(s):  
Age Groups ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Multiple Shoot Induction ◽  
Elephantopus Scaber ◽  
Cotyledonary Node Explants

An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoots per explant. The highest frequency of rooting (100%) and mean number of roots (3.3 per shoot) were observed when the shoots were transferred to MS medium supplemented with 1.0 mg L-1 IBA. The plantlets raised in vitro were acclimatized and transferred to soil with a 92% success rate. The protocol described here may be utilized for multiplication and conservation of elite clones of E. scaber.

scholarly journals Synthetic seeds - A novel approach for the conservation of endangered C. spiralis wt. and C. pusilla

2013 ◽  
Vol 48 (1) ◽  
pp. 39-42 ◽  
Author(s):  
KSR Murthy ◽  
MC Reddy ◽  
R Kondamudi
Keyword(s):  
Sodium Alginate ◽  
Multiple Shoot ◽  
Nutrient Composition ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Shoot Length ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Novel Approach ◽  
Alginate Matrix

In the present study best suitable explant for encapsulation and effect of nutrient composition of alginate matrix on regrowth performance of encapsulated explants of endangered C. spiralis and C. pusilla were studied. Among all the explants tested, good sprouting frequency was observed with shoot tips in both the species. Where as, multiple shoot induction with maximum shoot length was noticed with nodes. Sprouting frequency and viability of explants were reported in both the encapsulation mixtures tested. But, the significant increase in sprouting percentage, maximum number of shoots formed for each encapsulated explant and maximum shoot length were achieved with the encapsulation matrix prepared with Murashige and Skoog (MS) + 3 mg/L Benzyle amino purine (BAP) + 3 % sucrose + 3 % sodium alginate in both the species C. spiralis and C. pusilla. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15412 Bangladesh J. Sci. Ind. Res. 48(1), 39-42, 2013

scholarly journals In Vitro Mass Multiplication and Assessment of Genetic Stability of In Vitro Raised Artemisia absinthium L. Plants Using ISSR and SSAP Molecular Markers

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
B. Kour ◽  
G. Kour ◽  
S. Kaul ◽  
M. K. Dhar
Keyword(s):  
Molecular Markers ◽  
Genetic Stability ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Direct Regeneration ◽  
Naphthaleneacetic Acid ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Artemisia Absinthium

The present investigations were made attempting to develop a rapid, reliable, and reproducible in vitro regeneration protocol for Artemisia absinthium L., a medicinal plant of Kashmir Himalayas. Out of several auxin-cytokinin combinations tested, Murashige and Skoog’s (MS) medium supplemented with 0.5 mgL−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mgL−1 kinetin (Kn) was found to be the best for the callus induction. On the other hand, 4.5 mgL−1 6-benzylaminopurine (BAP) and 0.5 mgL−1 1-α-naphthaleneacetic acid (NAA) in the medium resulted in maximum shoot induction from the callus. Similarly, BAP and NAA at a concentration of 1.5 mgL−1 and 0.5 mgL−1, respectively, proved to be the best for the multiple shoot induction from nodal explants. Numerous shoots were obtained from nodal explants after third subculture. In vitro rooting was maximum on medium containing indole-3-butyric acid (IBA) at 0.5 mgL−1. The genetic stability of the in vitro raised plants of Artemisia absinthium was assessed using the intersimple sequence repeat (ISSR) and sequence-specific amplification polymorphism (SSAP) molecular markers. Both markers were able to detect the somaclonal variations in the callus regenerated plants, while no variation was detected in the plants regenerated from the nodal explants. SSAP has been found to be more useful in detection of variability as compared to ISSR molecular marker. The results of present study concluded that the direct regeneration protocol will be useful for the production of true to type plants of this medicinally important plant. This will go a long way in reducing the pressure on the natural populations for the secondary metabolite production, especially for extraction of essential oils.

scholarly journals Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

2013 ◽  
Vol 47 (4) ◽  
pp. 433-436
Author(s):  
Balasundaram Janarthanam ◽  
Sumathi Ethiraj
Keyword(s):  
Growth Response ◽  
Average Length ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Average Height ◽  
Ms Medium ◽  
Farm Yard Manure ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Indole 3 Acetic Acid

A protocol for multiple shoot induction and plant regeneration from nodal explants of Ocimum citriodorum has been developed. Nodal explants inoculated on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l Benzyl adenine (BAP) and 0.025 mg/l indole -3- acetic acid (IAA) showed better growth response (80%) and produced 15.2 ± 1.28 shoots per explant with an average length of 6.17 ± 0.29 cm after 35 days. Roots were induced after transfer to half strength MS medium supplemented with 0.5 mg/l Indole -3- butyric acid (IBA) produced 6.0 ± 1.0 roots with an average height of 4.9 ± 0.26 cm after 30 days. Plantlets with well developed root and shoot systems were successfully acclimated (80 %) and established in earthen pots containing mixture of soil, vermiculite and farm yard manure (1:1:1). Bangladesh J. Sci. Ind. Res. 47(4), 433-436, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.8547

scholarly journals DIRECT ORGANOGENESIS OF STEVIA REBAUDIANA IN VITRO USING NODAL EXPLANTS

AGROFOR ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Kasundi Mekhala GUNASENA ◽  
Sandun SENARATH
Keyword(s):  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Basal Medium ◽  
Stevia Rebaudiana ◽  
Multiple Shoot ◽  
Seedling Height ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Shoot Bud

Stevia rebaudiana Bertoni is a medicinal herb belonging to the family ofAsteraceae. It is a natural sweetener plant, which is estimated to be 300 timessweeter than cane sugar. In this study, reliable protocol was developed for directorganogenesis of S. rebaudiana using in vitro derived nodal explants. Seeds werecollected from mother plants and they were surface sterilized. To optimize thesurface sterilization procedure, dark color (fertile seeds) seeds were surfacesterilized using different concentrations and in different exposure time ofcarbendazim and sodium hypochlorite (Clorox). Out of different combinations0.2% carbendazim for 5 minutes, 10% sodium hypochlorite for 10 minutes and70% ethanol each followed by two successive washings in sterile distilled waterwas found to be the best for surface sterilization. Two sets of seeds (fresh, stored)were cultured on MS basal medium supplemented with different concentrations ofGA3 for seed germination. According to the results seed viability was lost withtime and it affected seed germination. Seed germination was not affected by GA3,but seedling height was affected by it. Seeds germinated on MS mediumsupplemented with 3.0 mg/L GA3 showed the highest seedling height after 10days. MS basal medium supplemented with different concentrations of BAP andKin were tested for shoot bud and multiple shoot induction. Out of different mediaMs basal medium supplemented with 2.0 mg/L BAP was found to be the bestmedium for shoot bud and multiple shoot induction within 60 days.

scholarly journals Alginate Encapsulation ofBegoniaMicroshoots for Short-Term Storage and Distribution

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Hamidou F. Sakhanokho ◽  
Cecil T. Pounders ◽  
Eugene K. Blythe
Keyword(s):  
Sodium Alginate ◽  
Calcium Chloride ◽  
Survival Rates ◽  
Synthetic Seeds ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Seed Formation ◽  
Significant Difference ◽  
Regenerated Plantlets

Synthetic seeds were formed from shoot tips of twoin vitrogrownBegoniacultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant combination into 100 mM calcium chloride (CaCl2·H2O) solution for 30 or 45 min. Both control and encapsulated shoots were transferred into sterile Petri dishes and stored at 4°C or 22°C for 0, 2, 4, 6, or 8 weeks. Conversion of synthetic seeds into plantlets for both storage environments was assessed in MS medium or peat-based substrate. No significant difference was found between the 30 and 45 min CaCl2·H2O treatments or the two cultivars. Encapsulation of explants improved survival rate over time irrespective of the medium type or storage environment. Survival rates of 88, 53, 28, and 11% for encapsulated microshoots versus 73, 13, 0, and 0% for control explants were achieved in microshoots stored for 2, 4, 6, and 8 weeks, respectively. The best results were obtained when synthetic seeds were stored at 4°C and germinated on MS medium. Regenerated plantlets were successfully established in potting soil.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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