scholarly journals ANTIBACTERIAL ACTIVITY OF METHANOLIC RHIZOME EXTRACT OF ALPINIA CALCARATA ROSC. (ZINGIBERACEAE)

2016 ◽  
Vol 3 (1) ◽  
pp. 65-66
Author(s):  
Arumugasamy K ◽  
Nantha Kumar R ◽  
Abdul Kaffoor H ◽  
Shalimol A
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The methanolic rhizome extract of A. calcarata was evaluated for its antibacterial activities against five bacterial strains Pseudomonas aeuroginosa, Proteus vulgaris, Salmonella paratyphi, Bacillus thurungiensis and Staphylococcus faccealis. The extract has inhibited all the tested bacterial species with different manner at various concentration. However the higher level zone of inhibition in 400 (mg/ml) is significant against all the above said bacterial strains of these Salmonella paratyphi. Based on the present study it can be conculuded that the plant rhizome possess potent anti bacterial activity.

Synthesis and Antibacterial Activities of Amidine Substituted Monocyclic β-Lactams

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijuan Zhai ◽  
Lili He ◽  
Yuanbai Liu ◽  
Ko Ko Myo ◽  
Zafar Iqbal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Lead Target ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Clinical Interest

Background: Mononcyclic β-lactams are regarded as the most resistant class of β-lactams against a series of β-lactamases though possess limited antibacterial activity. Aztreonam being the first clinically approved monobactam needs broad-spectrum efficacy through structural modification. Objective: We strive to synthesize a number of monocyclic β-lactams by varying the substituents at N1, C3 and C4 positions of azetidinone ring and study the antimicrobial effect on variable bacterial strains. Methods: Seven new monobactam derivatives 23a-g, containing substituted-amidine moieties linked to the azetidinone ring via thiazole linker, were synthesized through multistep synthesis. The final compounds were investigated for their in vitro antibacterial activities using broth microdilution method, against ten bacterial strains of clinical interest. The minimum inhibitory concentrations (MICs) of newly synthesized derivatives were compared with aztreonam, ceftazidime and meropenem, existing clinical antibiotics. Results: All compounds 23a-g showed higher antibacterial activities (MIC 0.25 µg/mL to 64 µg/mL) against tested strains as compared to aztreonam (MIC 16 µg/mL to >64 µg/mL) and ceftazidime (MIC >64 µg/mL). However all compounds, except 23d, exhibited lower antibacterial activity against all tested bacterial strains as compared to meropenem. Conclusion: Compound 23d showed comparable or improved antibacterial activity (MIC 0.25 µg/mL to 2 µg/mL) to meropenem (MIC 1 µg/mL to 2 µg/mL) in case of seven bacterial species. Therefore, compound 23d may be valuable lead target for further investigations against multi-drug resistant Gram-negative bacteria.

scholarly journals Analysis of Chemical Components and Antibacterial Activity in Essential Oil of Lantana Flowers (Lantana Camara L)

Biomedika ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 19-28
Author(s):  
Yulia Shara Sembiring ◽  
Cut Fatimah Zuhra
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Activities ◽  
Lantana Camara ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Chemical Components ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The purpose of this study was to evaluate the essential oil composition as well as antibacterial activities of essential oil of Lantana camara L flowers against four bacterial strains. Essential oil of Lantana camara L flowers was obtained by hydrodistillation method using Sthal and analyzed by GC-MS. The antibacterial activities of essential oil were tested by using disk diffusion method against four bacterial strains. Results showed  72 compounds for Lantana flowers essential oil, of which caryophyllene   (10,87%),   davanone   (9.84%),   a-humulene   (7.59%),   a-curcumene (3.35%), germacrene D (3.09%), calarene (2.42%), a-muurolene (2.27%), p-cymene (1.79%), 1,8 cineole (1.59%) , δ-cadinene (1.59%), a-copaene (1.12%), nerolidol B (1%) and ß-ocimene (0.54%) were detected as major components. Antibacterial activity assay of Lantana camara L flowers essential oil was examined against four bacterial strains including Gram (+and-) by using disk diffusion agar method. All the tested Gram (+and-) bacterial strains displayed slight to moderate antibacterial activity (7 – 10 mm) against all concentrations of essential oil. The essential oil of Lantana camara L flowers showed remarkable antibacterial activity with inhibition in concentrations of 10% and 20% against the phatogen bacterias Basillus subtilis (9.6 mm; 10.8 mm), Propionibacterium acnes ( 7.6 mm; 8.7 mm), Eschechia coli ( 8.4 mm ; 9.6 mm) and Pseudomonas aeruginosa ( 8.3 mm ; 9.3 mm) showed from zone of inhibition that was formed, zone of inhibition in concentration 20% was bigger than concentration 10%.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

Study of Antibacterial Activity of Nanosilver-Polypropylene Composite against Contaminated Bacteria in Molasses

2018 ◽  
Vol 939 ◽  
pp. 163-169
Author(s):  
Kittiya Pongsapasiritat ◽  
Nongnapat Jumnienkul ◽  
Rapeepun Dangtungee ◽  
Malinee Sriariyanun
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Research Field ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Polypropylene Composite ◽  
Nanosilver Particles ◽  
Sugar Degradation

The nanosilver particles are attractive in the research field of nanotechnology due to one of their applications as an effective antibacterial agent. The main objective of the current research is to inhibit the bacterial growth in the molasses to decrease the rate of total sugar degradation caused by contaminated bacteria. The polypropylene masterbatch containing with AgNPs (PP/AgNPs) was prepared and tested against contaminated bacterial species identified in molasse. The percentage of bacterial inhibition was calculated by observing the viability of bacteria cultures by using spread plate technique after treated with PP/AgNPs masterbatch in batch experiment. The concentrations of nanoparticles in masterbatch were varied to be 200, 500, 1000, 1200 and 1500 ppm. At 1500 ppm, the compromised antibacterial activities were observed in all tested bacterial species with 2-h-long exposure time. The results in this study could be further applied to fit in operation of molasse use in industrial scale in various application, including bioethanol production.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals Synthesis and Antibacterial Activity of 3-(Substituted)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Ramgopal Appani ◽  
Baburao Bhukya ◽  
Kiran Gangarapu
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Primary Amines ◽  
Reference Standard ◽  
Proteus Vulgaris ◽  
Zone Of Inhibition ◽  
Active Compounds ◽  
Multistep Synthesis

A series of novel 3-(substituted)-2-(substituted quinazolinylamino)quinazolin-4(3H)-ones were synthesized by the reaction of 3-(substituted)-2-hydrazino-quinazoline-4(3H)-ones with 2-phenyl-3,1-benzoxazin-4-one. The starting materials 3-(substituted)-2-hydrazino-quinazolin-4(3H)-ones were synthesized from various primary amines by a multistep synthesis. All the title compounds were tested for their antibacterial activity using ciprofloxacin as reference standard. Compounds 3-(4-fluorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (9a) and 3-(4-chlorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (9h) emerged as the most active compounds of the series. These compounds have shown most potent antibacterial activity against the tested organisms ofProteus vulgarisandBacillus subtilishaving zone of inhibition values of 1.1 cm and 1.4 cm for compound9a1.2 cm and 1.0 cm for compound9h, respectively.

scholarly journals COMPARATIVE ANALYSIS OF METAL NANOPARTICLES SYNTHESIZED FROM HIBISCUS ROSA SINESIS AND THEIR ANTIBACTERIAL ACTIVITY ESTIMATION AGAINST NINE PATHOGENIC BACTERIA

2017 ◽  
Vol 10 (5) ◽  
pp. 323
Author(s):  
Shruti Tyagi
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Au Nanoparticles ◽  
Size Range ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Agar Disc ◽  
Vis Spectroscopy

Objective: This study demonstates  a simple, cost effective protocol  for biosynthesis of stable silver (Ag) and gold (Au) nanoparticles from Hibiscus Rosa sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: Silver (Ag) and gold (Au) nanoparticles were biosynthesized from Hibiscus Rosa sinesis were characterized by UV–VIS spectroscopy, FTIR and TEM. The antibacterial activities  of AgNPs  and AuNPs were evaluated against  9 pathogenic bacterial species  Pseudomonas aeroginosa, Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogens, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Synthesized AgNPs  were obtained in 13.01 to 28.14 nm size range, while AuNPs were in  6.32 to 18.19 nm size range. The results of Fourier transform infrared spectroscopy (FTIR) spectra indicates  that the AuNPs are bound to amine groups and the AgNPs to carboxylate ion groups. The antibacterial activities  of AgNPs,  the zone of inhibition significantly increased with the  increases of concentrations of AgNPs in all pathogenic bacterial species  except  in the case of S. epidermidis at 50%, S. aerogenes and A. hydrophila at 70%, while in case of AuNPs antibacterial activity  was displayed  only against B. subtilis at 20% and 100% concentration.Conclusion: This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from Hibiscus Rosa sinensis leaf extract and insights to their potential applicability as an alternative antibacterial  agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria. Keywords: silver nanoparticles; gold  nanoparticles; UV–VIS spectroscopy; FTIR; TEM; antibacterial activities.  

Antibacterial Activities of Antibacterial Proteins/Peptides Isolated from Organs and Mucus of Clarias Gariepinus Reared at High Stocking Density

2012 ◽  
Vol 455-456 ◽  
pp. 455-460 ◽  
Author(s):  
Xiao Mei Wang ◽  
Wei Dai ◽  
Ke Zhing Xing ◽  
Tian Jun Li ◽  
Xin Wang
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Crude Protein ◽  
Clarias Gariepinus ◽  
Gel Filtration ◽  
Stocking Density ◽  
Antibacterial Activities ◽  
Edwardsiella Tarda ◽  
Bacterial Strains ◽  
Sds Page

. Antibacterial proteins/peptides are important parts of the innate immune system in Clarias gariepinus. To examine potential antibacterial proteins/peptides in organs and mucus of C. gariepinus, crude protein/peptide extracts were isolated with ammonium sulfate precipitation from mucus, skin, gill, suprabranchial organ and intestine. Following further extraction using Sephadex G-50 gel filtration chromatography, the proteins/peptides associated with two absorption peaks (AP1 and AP2) were pooled, respectively, and assayed for their antibacterial activities against Escherichia coli, Aeromonas hydrophila and Edwardsiella tarda. The results showed that AP1 and AP2 from all the sampled tissues and mucus at concentration of 100 mg mL-1 exhibited antibacterial activity against the tested bacterial strains. Differences in antibacterial activity were observed among sample extracts. The protein profiles of AP1 obtained by Tricine-SDS-PAGE gel showed a broad range of peptides/proteins, and molecular weight of the mutual abundant peptide obtained was about 27 kDa. Antibacterial activity of AP2 extracted from intestine was due to peptide with molecular weight of 5.5 kDa.

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