Histological Classification in Canine Mammary Tumors: Back to Basics

2021 ◽  
Vol 04 (03) ◽  
Author(s):  
Aline Alvarenga da Rocha ◽  
Maria de Fátima Gartner
Keyword(s):  
Mammary Tumors ◽  
Histological Classification ◽  
Canine Mammary Tumors

scholarly journals Overexpression of Mucin 1 Suppresses the Therapeutical Efficacy of Disulfiram against Canine Mammary Tumor

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 37
Author(s):  
Ying Zhao ◽  
Zixiang Lin ◽  
Zhaoyan Lin ◽  
Chaoyu Zhou ◽  
Gang Liu ◽  
...  
Keyword(s):  
Mammary Tumor ◽  
Mammary Tumors ◽  
Expression Level ◽  
Canine Mammary Tumor ◽  
Wild Type ◽  
Mucin 1 ◽  
Mammary Tumor Cell ◽  
Mammary Tumor Cell Line ◽  
Canine Mammary Tumors ◽  
Wild Type Control

Mucin 1 (MUC1), a transmembrane protein, is closely associated with the malignancy and metastasis of canine mammary tumors; however, the role of overexpressed MUC1 in the development of cancer cells and response to drug treatment remains unclear. To address this question, we developed a new canine mammary tumor cell line, CIPp-MUC1, with an elevated expression level of MUC1. In vitro studies showed that CIPp-MUC1 cells are superior in proliferation and migration than wild-type control, which was associated with the upregulation of PI3K, p-Akt, mTOR, Bcl-2. In addition, overexpression of MUC1 in CIPp-MUC1 cells inhibited the suppressing activity of disulfiram on the growth and metastasis of tumor cells, as well as inhibiting the pro-apoptotic effect of disulfiram. In vivo studies, on the other side, showed more rapid tumor growth and stronger resistance to disulfiram treatment in CIPp-MUC1 xenograft mice than in wild-type control. In conclusion, our study demonstrated the importance of MUC1 in affecting the therapeutical efficiency of disulfiram against canine mammary tumors, indicating that the expression level of MUC1 should be considered for clinical use of disulfiram or other drugs targeting PI3K/Akt pathway.

Progesterone receptor membrane component 1 (PGRMC1) expression in canine mammary tumors: A preliminary study

2020 ◽  
Vol 132 ◽  
pp. 101-107
Author(s):  
Laura Terzaghi ◽  
Barbara Banco ◽  
Debora Groppetti ◽  
Priscila C. Dall'Acqua ◽  
Chiara Giudice ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Mammary Tumors ◽  
Membrane Component ◽  
Canine Mammary Tumors ◽  
Receptor Membrane ◽  
Preliminary Study

scholarly journals Localized Amyloidosis in Canine Mammary Tumors

2000 ◽  
Vol 37 (1) ◽  
pp. 104-107 ◽  
Author(s):  
H. Taniyama ◽  
A. Kitamura ◽  
Y. Kagawa ◽  
K. Hirayama ◽  
T. Yoshino ◽  
...  
Keyword(s):  
Mammary Tumors ◽  
Canine Mammary Tumors ◽  
Localized Amyloidosis

scholarly journals Tumor-associated Carbohydrate Antigens: Sialyl Leaand T/Tn Antigens in Canine Mammary Tumors

2009 ◽  
Vol 46 (2) ◽  
pp. 222-226 ◽  
Author(s):  
M. Nowak ◽  
J. Madej ◽  
P. Dziégiel ◽  
W. Łopuszyński ◽  
A. Rodo ◽  
...  
Keyword(s):  
Mammary Tumors ◽  
Carbohydrate Antigens ◽  
Canine Mammary Tumors ◽  
Tumor Associated Carbohydrate Antigens

Involvement of Nestin in the Progression of Canine Mammary Carcinoma

2021 ◽  
pp. 030098582110186
Author(s):  
Hisashi Yoshimura ◽  
Maiko Moriya ◽  
Ayaka Yoshida ◽  
Masami Yamamoto ◽  
Yukino Machida ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Carcinoma ◽  
Mammary Tumors ◽  
Carcinoma Cells ◽  
Nestin Expression ◽  
Mammary Carcinomas ◽  
Canine Mammary Carcinoma ◽  
Canine Mammary Tumors ◽  
Mammary Carcinoma Cells ◽  
Luminal Epithelial Cells

Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.

Veterinary approaches to canine mammary tumors and knowledge of the consensus statement in Brazil

2021 ◽  
Vol 14 (1) ◽  
pp. 24-28
Author(s):  
Tainá Zuchi ◽  
◽  
Claudia Lopatini ◽  
Joice Faria
Keyword(s):  
Consensus Statement ◽  
Immunohistochemical Analysis ◽  
Mammary Tumors ◽  
Surgical Removal ◽  
Therapeutic Approaches ◽  
Canine Mammary Tumors ◽  
Preventive Actions ◽  
Criteria For Diagnosis ◽  
Unilateral Mastectomy

Mammary gland tumors are one of the most commonly diagnosed tumors in female dogs, with a reported prevalence ranging from 26 to 73% in Brazil. In recognition of the importance of these tumors veterinary researchers and clinicians in Brazil produced the first consensus statement regarding canine mammary tumors in 2010. The intention was to establish criteria for diagnosis, prognosis, and treatment. This study evaluated the methods of prevention, diagnosis, treatment, and determination of prognosis used by veterinarians in Brazil, and sought to quantify the number of veterinarians who were aware of the consensus statement. One hundred and three veterinary clinics participated in the study, 87.37% of which recommend early neutering as a preventative treatment for mammary tumors. For diagnosis, 100% of these use laboratory testing, 94.17% perform chest radiography, 78.64% incisional biopsies, 44.66% cytological analysis, and 13.59% immunohistochemical analysis. The most common surgical procedure is unilateral mastectomy (72.81%), and chemotherapy is performed in 49.51% of the clinics. Of the 103 clinics, 66.01% were aware of the consensus. Although knowledge of the consensus statement is widespread among veterinarians in Brazil, not all its recommendations are being followed. Preventive actions for canine mammary tumors are well established in most parts of the country. However, the consensus statement has had little influence on informing prognostic and therapeutic approaches, with a poor uptake of surgical removal of lymph nodes and immunohistochemical examination.

Methylation pattern and mutational status of BRCA1 in canine mammary tumors in a Brazilian population

2018 ◽  
Vol 28 (1) ◽  
pp. 63-67
Author(s):  
Verena da Costa Ferreira ◽  
Danilo do Rosário Pinheiro ◽  
Raissa Melo de Sousa ◽  
Lucien Roberta Valente Miranda de Aguirra ◽  
Washington Luiz Assunção Pereira ◽  
...  
Keyword(s):  
Mammary Tumors ◽  
Methylation Pattern ◽  
Brazilian Population ◽  
Canine Mammary Tumors ◽  
Mutational Status

scholarly journals CD44 and CD24 Expression and Prognostic Significance in Canine Mammary Tumors

2018 ◽  
Vol 56 (3) ◽  
pp. 377-388 ◽  
Author(s):  
Bernadette Rogez ◽  
Quentin Pascal ◽  
Audrey Bobillier ◽  
François Machuron ◽  
Chann Lagadec ◽  
...  
Keyword(s):  
Overall Survival ◽  
Prognostic Significance ◽  
Mammary Tumors ◽  
Clinicopathological Parameters ◽  
Tumor Type ◽  
High Grade ◽  
Mammary Carcinomas ◽  
Canine Mammary Tumors ◽  
Valuable Marker ◽  
High Level

CD44+/CD24– phenotype has been used to identify human and canine mammary cancer stem-like cells. In canine mammary tumors, CD44+/CD24– phenotype has been associated with high grade and lymph node infiltration. However, several studies have reported opposing results regarding the clinical significance of phenotypic groups formed by the combination of CD44 and CD24 in both human and canine mammary tumors. So far, no study has investigated the correlation between these phenotypes and survival in dogs. The aim of this study was to investigate the expression and distribution of CD44 and CD24 in canine mammary carcinomas and to correlate them with histological diagnosis and survival in a well-characterized cohort. Immunohistochemistry was performed in 96 mammary carcinomas with antibodies against CD44 and CD24. Expression of CD44+ and CD44+/CD24– phenotype was detected in 75 of 96 (78%) and 63 of 96 (65.6%) carcinomas, respectively. Their expression was associated with tumor type, occurring more often in tubular complex carcinomas than in solid carcinomas. CD44+/CD24– phenotype was associated with a better overall survival ( P = .001). CD24+ expression was detected in 52 of 96 tumors (54%) and CD44–/CD24+ phenotype in 39 of 96 tumors (40.6%). Both were associated with poor clinicopathological parameters (high grade, and emboli). No correlation with overall survival was observed. CD44+/CD24– expression was associated with a better prognosis and occurred at high frequency and high level, indicating that this phenotype is not suitable to detect cancer stem cells in canine mammary carcinomas. Although further studies are needed, our results suggest that CD24 may constitute a valuable marker of poor prognosis for canine mammary carcinomas.

Sign in / Sign up

Export Citation Format

Share Document