scholarly journals Histomorphometric evaluation of the effects of local application of red cloveroil (trifolium pratense) on bone healing in rats

2020 ◽  
Vol 32 (2) ◽  
pp. 26-31
Author(s):  
Nada AL-Ghaban ◽  
Ghazwan Jasem
Keyword(s):  
Bone Marrow ◽  
Bone Healing ◽  
Trifolium Pratense ◽  
Bone Cells ◽  
Red Clover ◽  
Gelatin Sponge ◽  
Bony Defect ◽  
Marrow Space ◽  
And Control ◽  
The Impact

Background: Red clover oil (Trifolium pratense) has isoflavones bunches which have estrogen-like exercises and may establish an option in contrast to hormone substitution treatment. The present study investigated the impact of Red clover oil on bone healing in rats by histomorphometric study. Materials and Methods: Intra bony defect was performed in right femur of thirty six healthy male albino rats. Then these rats were randomly divided into three groups (12 rats each): one control and 2 experimental groups. For control, the bony defect was left for normal healing; for the experimental (S) group, the defect was treated with hemostatic absorbable gelatin sponge; and for experimental (RS) group, the bony defect was treated with 0.2 ml red clover oil and covered by haemostatic absorbable gelatin sponge. Six rats from each group were sacrificed at 2 and 4 weeks intervals. Histomorphometric analysis was performed on H&E bone section of all the studied groups which includes counting of bone cells (osteoblasts, osteocytes and osteoclasts), trabecular number, trabecular area and bone marrow space area. Results: Histomorphometric results of bone cells revealed that the combination group stimulated larger numbers of osteoblasts and osteocytes than in sponge and control group. Number of new bone trabeculae, trabecular area and bone marrow space area showed higher mean values in combination groups than others. Highly significant differences between groups were observed in all histomorphometric parameters throughout all durations. Conclusion: Red clover oil stimulated larger numbers of osteoblasts and osteoclasts, indicating increased bone remodeling especially at 2 weeks interval as compared with sponge and control groups. Key words: Bone defect, Red clover, Rats.

The Effects of Bone Chips Dehydrated with Solvent on Healing Bone Defects

2002 ◽  
Vol 30 (2) ◽  
pp. 168-173 ◽  
Author(s):  
E Dayi ◽  
M Aslan ◽  
G Şimşek ◽  
AB Yilmaz
Keyword(s):  
Bone Marrow ◽  
Cross Sections ◽  
Bone Healing ◽  
Bone Grafts ◽  
Bone Defects ◽  
Control Groups ◽  
Bone Cortex ◽  
Healing Bone ◽  
The Right ◽  
And Control

The effect of bone chips dehydrated with solvent on the healing of bone defects was evaluated. Solvent-dehydrated spongiose bone chips were placed in experimentally formed cavities in the right back tibia of rabbits. After 10, 20 and 30 days, histopathological cross-sections from the bone grafts were examined microscopically for bone healing and formation of spongiose bone, cortex and bone marrow. Spongiose bone chips had a positive and accelerating influence on the healing of bone defects in the 10-day period after transplantation, but no significant differences were observed between the treated and control groups 20 and 30 days after transplantation.

scholarly journals Changes in yield and nutritive value of red clover (Trifolium pratense L.) and Festulolium (Festulolium braunii (K. Richt) A. Camus) under drought stress

2019 ◽  
Vol 28 (1) ◽  
Author(s):  
Mariola Staniak
Keyword(s):  
Drought Stress ◽  
Nutritive Value ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Pure Stand ◽  
Crude Fibre ◽  
Influence Of Water ◽  
The Impact

The aim of the research was to assess the impact of water stress on the yield and content of basic nutrients in the biomass of Trifolium pratense and Festulolium braunii cultivated in pure stand and in mixture. A pot experiment was carried out in 2012–2014, at two levels of soil moisture: well-watered and drought stress. The study showed that stress significantly reduced the dry matter yield (DMY), the most in T. pratense, followed by mixture, and F. braunii. The effect of drought stress on the nutritive value was considerable less pronounced than the influence on DMY. No significant influence of water deficit on crude protein, crude fibre and crude ash contents was found, but only on water-soluble carbohydrate and crude fat content in one year of the study. It was found, that under drought stress T. pratense is more suitable for cultivation in the mixture with F. braunii than in pure stand, due to a lower reduction of DMY and no adverse effect of water deficiency on yield quality.

scholarly journals The Response of Red Clover (Trifolium pratense L.) to Separate and Mixed Inoculations with Rhizobium leguminosarum and Azospirillum brasilense in Presence of Polycyclic Aromatic Hydrocarbons

2020 ◽  
Vol 17 (16) ◽  
pp. 5751
Author(s):  
Karolina Furtak ◽  
Karolina Gawryjołek ◽  
Anna Gałązka ◽  
Jarosław Grządziel
Keyword(s):  
Azospirillum Brasilense ◽  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Shoot Biomass ◽  
Polycyclic Aromatic ◽  
Trifolium Pratense L ◽  
The Impact ◽  
Positive Effect ◽  
Root And Shoot Biomass

This study aimed to evaluate the impact of co-inoculation Rhizobium sp. and Azospirillum sp. on plant (Trifolium pratense L.) growth in the presence of polycyclic aromatic hydrocarbon (PAH) contamination (anthracene, phenanthrene, and pyrene). Eight strains from the genus Rhizobium leguminosarum bv. trifolii were selected for biotest analysis. Two methods of inoculation were used in the chamber experiment: (1) R. leguminosarum alone and (2) a combined inoculant (R. leguminosarum and Azospirillum brasilense). For comparison, non-contaminated controls were also used. The results demonstrated that co-inoculation of plants with Rhizobium and Azospirillum resulted in more root and shoot biomass than in plants inoculated with R. leguminosarum alone. The results indicated that application of a co-inoculation of bacteria from Rhizobium and Azospirillum species had a positive effect on clover nodulation and growth under the condition of PAH contamination.

MDS1-EVI1 and EVI1 Overexpression Results in Changes in the Behavior of Murine Hematopoietic Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1452-1452
Author(s):  
Jean-Yves Metais ◽  
Rotraud Wieser ◽  
Cynthia E. Dunbar
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Hematopoietic Cells ◽  
Gene Products ◽  
Gene Complex ◽  
Murine Bone Marrow ◽  
And Control ◽  
The Impact ◽  
Marrow Cells

Abstract We have found non-random patterns of retroviral integration in long-term hematopoietic repopulating cells in the rhesus macaque, with frequent integration events of MLV vectors into the MDS1-EVI1 gene complex. These findings, along with reports regarding frequent integration events in the same gene complex in patients with chronic granulomatous disease receiving MLV-transduced hematopoietic cells in a clinical trial and the ability of MLV vectors activating expression of this gene via integration to immortalize primary murine bone marrow cells, suggests these gene products could have important roles in normal and leukemic hematopoiesis. Expression from this gene complex can result in translation of at least three distinct proteins: MDS1, EVI1, and MDS1-EVI1. EVI1 has been the most studied protein of this locus. Its overexpression, as a consequence of chromosomal rearrangement or viral integration, is associated with leukemia. MDS1-EVI1 contains a PR domain that is lacking in EVI1 and is thought to possibly be antagonistic to EVI1, however the location of the integrations in our prior rhesus studies would indicate that overexpression of either gene product could be immortalizing. Both proteins share the same expression profile in normal tissues as well as most reports of myeloid leukemias. To investigate the impact of the three gene products on hematopoietic cells, we cloned murine mds1, evi1, and mds1-evi1 into the pMIEV-GFP retroviral vector and produced ecotropic vector particles. These were used to transduce the murine BaF3 hematopoietic cell line as a model to study the impact of expression of these various gene products. Gene expression analysis using Afflymetrix arrays demonstrated that both EVI1 and MDS1-EVI1 expression produced dramatic changes in gene expression profiles of these cells, compared to MDS1 and control vector. For instance, EVI1 transduced cells overexpressed oncogenes such as small G proteins belonging to the RAS family. There was modulation of genes implied in hematopoiesis, apoptosis, TGF beta signaling, and cell cycle. To assess changes in cell cycling of transduced BaF3 cells we used a flow cytometric assay, which unraveled an arrest in G1 phase only when EVI1 was overexpressed. These changes were concomitant to an increased metabolic activity as measured by an MTT assay. Further studies of these different pathways have to be performed in order to confirm the results obtained by the DNA chips analysis. Primary murine bone marrow cells could be immortalized after transduction by both EVI1 and MDS1-EVI1 vectors, compared to MDS1 and control vectors. Mice have been transplanted with primary bone marrow cells transduced with all vectors, and are being followed for hematopoietic changes or leukemia. In conclusion, both MDS1-EVI1 and EVI1 overexpression appear to result in marked changes in the behavior of primitive hematopoietic cells.

scholarly journals Transcriptional changes suggest a major involvement of Gibberellins in Trifolium pratense regrowth after mowing

2019 ◽  
Author(s):  
Denise Brigitte Herbert ◽  
Thomas Gross ◽  
Oliver Rupp ◽  
Annette Becker
Keyword(s):  
Morphological Traits ◽  
Nutritional Value ◽  
Molecular Mechanisms ◽  
Trifolium Pratense ◽  
Molecular Genetic ◽  
Red Clover ◽  
Genetic Network ◽  
Genetic Response ◽  
Transcriptional Changes ◽  
And Control

AbstractRed clover (Trifolium pratense) is used worldwide as a fodder plant due its high nutritional value. In response to mowing, red clover exhibits specific morphological traits to compensate the loss of biomass. The morphological reaction is well described, but knowledge of the underlying molecular mechanisms are still lacking. Here we characterize the molecular genetic response to mowing of red clover by using comparative transcriptomics in greenhouse conditions and agriculturally used field. The analysis of mown and control plants revealed candidate genes possibly regulating crucial steps of the genetic network governing the regrowth reaction. In addition, multiple identified gibberellic acid (GA) related genes suggest a major role for GA in establishing the regrowth morphology of red clover. Mown red clover plants showing this regrowth morphology were partially “rescued” by exogenous GA application, demonstrating the influence of GA during regrowth. Our findings provide insights into the physiological and genetic processes of mowing red clover, to serve as a base for red clover yield improvement.

Etude des effets phytoestrogéniques et phytotoxiques de la végétation de milieux ouverts sur la souris de laboratoire

1980 ◽  
Vol 58 (9) ◽  
pp. 1575-1581 ◽  
Author(s):  
Jean-Marie Bergeron ◽  
Marie Goulet
Keyword(s):  
Population Dynamics ◽  
Natural Environment ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Land Plants ◽  
Experimental Animals ◽  
Uterine Growth ◽  
Open Land ◽  
Estrogenic Properties ◽  
The Impact

Dietary experiments have been designed to emphasize the phytoestrogenic and phytotoxic effects of certain open-land plants. The 30 plant species given as food did delay the growth of the animals. Some nine species of plant, including red clover (Trifolium pratense), have actually accelerated uterine growth in experimental animals. Moreover, 12 species produced an atrophy of the kidneys; some of those species also possess estrogenic properties. Some of those plants are already known for their estrogenic or toxic effects, but most of the plants used seem to have some effect. We examined the possibility of using this experimental method in a study of population dynamics of wild small mammals in order to determine the impact of the plants in a natural environment.[Journal Translation]

Herbicides for Renovation of Pastures and Control of Tall Ironweed (Vernonia altissima)

Weed Science ◽  
1979 ◽  
Vol 27 (3) ◽  
pp. 342-345 ◽  
Author(s):  
E. J. Peters ◽  
S. A. Lowance
Keyword(s):  
Acetic Acid ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Perennial Weeds ◽  
Dichlorophenoxy Acetic Acid ◽  
And Control

August and October applications of glyphosate [N-(phosphonomethyl)glycine] and paraquat (1,1′-dimethyl-4,4′-bipyridinium ion) were compared at two rates for pasture renovation. During the 2 yr of the experiment, sod suppression was frequently greater with October applications than with August applications. Medium red clover (Trifolium pratense L.) became established in all the plots, and density and yield tended to increase as grass suppression increased.Glyphosate was compared over a 3-yr period with an ester of 2,4-D [(2,4-dichlorophenoxy)acetic acid] and picloram (4-amino-3,5,6-trichloropicolinic acid) for control of tall ironweed (Vernonia altissima Nutt.) when applied in August. Picloram killed from about 80 to 100% of the tall ironweed plants and was superior to 2,4-D. Glyphosate was inconsistent for controlling tall ironweed but could be used for killing perennial weeds as well as sod suppression.

scholarly journals MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells

2018 ◽  
Vol 74 (9) ◽  
pp. 1368-1374 ◽  
Author(s):  
Sudharsan Periyasamy-Thandavan ◽  
John Burke ◽  
Bharati Mendhe ◽  
Galina Kondrikova ◽  
Ravindra Kolhe ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Cells ◽  
Marrow Stromal Cells ◽  
Luciferase Reporter ◽  
Bm Niche ◽  
Human Bmscs ◽  
Age Dependent ◽  
The Impact

Abstract Stromal cell-derived factor-1 (SDF-1 or CXCL12) is a cytokine secreted by cells including bone marrow stromal cells (BMSCs). SDF-1 plays a vital role in BMSC migration, survival, and differentiation. Our group previously reported the role of SDF-1 in osteogenic differentiation in vitro and bone formation in vivo; however, our understanding of the post-transcriptional regulatory mechanism of SDF-1 remains poor. MicroRNAs are small noncoding RNAs that post-transcriptionally regulate the messenger RNAs (mRNAs) of protein-coding genes. In this study, we aimed to investigate the impact of miR-141-3p on SDF-1 expression in BMSCs and its importance in the aging bone marrow (BM) microenvironment. Our data demonstrated that murine and human BMSCs expressed miR-141-3p that repressed SDF-1 gene expression at the functional level (luciferase reporter assay) by targeting the 3′-untranslated region of mRNA. We also found that transfection of miR-141-3p decreased osteogenic markers in human BMSCs. Our results demonstrate that miR-141-3p expression increases with age, while SDF-1 decreases in both the human and mouse BM niche. Taken together, these results support that miR-141-3p is a novel regulator of SDF-1 in bone cells and plays an important role in the age-dependent pathophysiology of murine and human BM niche.

Marrow Adipocytes: Origin, Structure, and Function

2020 ◽  
Vol 82 (1) ◽  
pp. 461-484 ◽  
Author(s):  
Francisco J.A. de Paula ◽  
Clifford J. Rosen
Keyword(s):  
Bone Marrow ◽  
Bone Cells ◽  
Whole Body ◽  
Postnatal Life ◽  
Therapeutic Approaches ◽  
Age Related ◽  
Hypoxic Environment ◽  
Marrow Space ◽  
Adipocyte Progenitors ◽  
And Function

The skeleton harbors an array of lineage cells that have an essential role in whole body homeostasis. Adipocytes start the colonization of marrow space early in postnatal life, expanding progressively and influencing other components of the bone marrow through paracrine signaling. In this unique, closed, and hypoxic environment close to the endosteal surface and adjacent to the microvascular space the marrow adipocyte can store or provide energy, secrete adipokines, and target neighboring bone cells. Adipocyte progenitors can also migrate from the bone marrow to populate white adipose tissue, a process that accelerates during weight gain. The marrow adipocyte also has an endocrine role in whole body homeostasis through its varied secretome that targets distant adipose depots, skeletal muscle, and the nervous system. Further insights into the biology of this unique and versatile cell will undoubtedly lead to novel therapeutic approaches to metabolic and age-related disorders such as osteoporosis and diabetes mellitus.

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