scholarly journals Comparison of BASP1 and MARCKS protein exchange traits in the kidneys of rats with spontaneous

2020 ◽  
pp. 19-26
Author(s):  
Анна Сергеевна Альдекеева
Keyword(s):  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Cortical Layers ◽  
Hypertensive Rats ◽  
Tissue Samples ◽  
Spontaneously Hypertensive ◽  
Expression Levels ◽  
Interlinear Differences ◽  
Mrna Expression Levels ◽  
Marcks Protein

Исследовали возможные различия в обмене белков BASP1 и MARCKS в почках крыс со спонтанной гипертензией (линияSHR), путем сравнения уровней экспрессии мРНК этих белков в различных структурах почек. Работа выполнена на самцах крыс линии SHR (n=8) и крыс линии WKY (n=8), в возрасте 90 дней. Исследование проводили на образцах ткани из коркового и мозгового слоев почек. Уровни экспрессии мРНК BASP1 и MARCKS определяли методом ПЦР в реальном времени. Обнаружены достоверные различия (p<0.001) уровней экспрессии мРНК BASP1 и MARCKS между корковыми и мозговыми слоями почек у крыс линий SHR и WKY. При этом межлинейные различия в уровнях экспрессии мРНК обоих белков практически отсутствовали (p=0.050). Показано, что неравномерное распределение уровней экспрессии мРНКбелков BASP1 и MARCKS в мозговом и корковом слоях почек не зависит от уровня артериального давления и наличия спонтанной гипертензии This study aims to detect differences in BASP1 and MARCKS protein metabolisms in kidneys of spontaneously hypertensive rats by comparing their mRNA expression in different kidney structures. The work was performed on 90 days old male SHR (n = 8) and WKY (n = 8) rats. The study was carried out on tissue samples from cortical and medullar kidney layers. BASP1 and MARCKS mRNA expression levels were determined by real-time PCR. Significant differences (p <0.001) in the expression levels of BASP1 and MARCKS mRNA between cortical and medullar kidney layers in both SHR and WKY strain rats were found. Moreover, interlinear differences in the levels of both proteins’ mRNA expression were practically absent (p = 0.050). It was shown that uneven distribution of mRNA expression levels of BASP1 and MARCKS mRNA in kidneys medullar and cortical layers does not depend on the level of blood pressure and the presence of spontaneous hypertension.

scholarly journals NAP-22 and MARCKS mRNA expression levels in spontaneously hypertensive rat kidneys: the effect of drinking water with different calcium contents

2021 ◽  
Vol 27 (4) ◽  
pp. 436-445
Author(s):  
A. S. Aldekeeva ◽  
A. Y. Plekhanov ◽  
N. Z. Klyueva
Keyword(s):  
Drinking Water ◽  
Mrna Expression ◽  
Calcium Intake ◽  
Intracellular Signaling ◽  
Spontaneously Hypertensive Rats ◽  
Spontaneously Hypertensive Rat ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Expression Levels ◽  
Mrna Expression Levels

Objective. The aim of study was to assess the effect of the level of calcium intake with drinking water on NAP-22 and MARCKS mRNA expression in cortical and medullar kidney layers of spontaneously hypertensive rats.Design and methods. The study involved 90-day-old SHR (n = 8) and WKY (n = 8) strain rats of both sexes. We assessed tissue samples from cortical and medullar kidney layers. NAP-22 and MARCKS mRNA expression levels were determined by RT-PCR.Results. Sufficient drinking water calcium intake was associated with similar the expression of NAP-22 and MARCKS mRNA in kidneys of spontaneously hypertensive and normotensive rats. Consumption of drinking water with insufficient calcium content it decreases in both rat strains, being more evident in spontaneously hypertensive rats, especially in the medullar layer.Conclusions. Our results show that genetically determined impairments of calcium metabolism in cells of spontaneously-hypertensive rats (SHR line) and their effect on intracellular signaling processes are more evident with the reduced intake of exogenous calcium.

scholarly journals NAP-22 and MARCKS mRNA expression levels in spontaneously hypertensive rat kidneys: the effect of drinking water with different calcium contents

2021 ◽  
Vol 27 (4) ◽  
pp. 436-445
Author(s):  
A. S. Aldekeeva ◽  
A. Y. Plekhanov ◽  
N. Z. Klyueva
Keyword(s):  
Drinking Water ◽  
Mrna Expression ◽  
Calcium Intake ◽  
Intracellular Signaling ◽  
Spontaneously Hypertensive Rats ◽  
Spontaneously Hypertensive Rat ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Expression Levels ◽  
Mrna Expression Levels

Objective. The aim of study was to assess the effect of the level of calcium intake with drinking water on NAP-22 and MARCKS mRNA expression in cortical and medullar kidney layers of spontaneously hypertensive rats.Design and methods. The study involved 90-day-old SHR (n = 8) and WKY (n = 8) strain rats of both sexes. We assessed tissue samples from cortical and medullar kidney layers. NAP-22 and MARCKS mRNA expression levels were determined by RT-PCR.Results. Sufficient drinking water calcium intake was associated with similar the expression of NAP-22 and MARCKS mRNA in kidneys of spontaneously hypertensive and normotensive rats. Consumption of drinking water with insufficient calcium content it decreases in both rat strains, being more evident in spontaneously hypertensive rats, especially in the medullar layer.Conclusions. Our results show that genetically determined impairments of calcium metabolism in cells of spontaneously-hypertensive rats (SHR line) and their effect on intracellular signaling processes are more evident with the reduced intake of exogenous calcium.

Changes of Blood Pressure Rhythm and Clock Protein Expression Levels in Spontaneously Hypertensive Rats After Ischemia-Reperfusion

2021 ◽  
Author(s):  
Jing Jin ◽  
Yumeng Liu ◽  
Jing Huang ◽  
Dong Zhang ◽  
Jian Ge ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Protein Expression ◽  
Circadian Clock ◽  
Spontaneously Hypertensive Rats ◽  
Ischemia Reperfusion ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Expression Levels ◽  
The Relationship ◽  
Clock Protein

Abstract Objective A variety of circadian patterns of blood pressure after ischemic stroke in patients with essential hypertension appear to be a potential risk of stroke recurrence, but the mechanism is still unclear. This study intends to reveal the changes in blood pressure rhythm and circadian clock protein expression levels in spontaneously hypertensive rats (SHR) after ischemia-reperfusion, and the relationship between the two. Methods Using the SHR middle cerebral artery occlusion experimental model, the systolic blood pressure was continuously monitored for 24 hours after the operation to observe the blood pressure rhythm. The rat tail vein blood was taken every 3h, and the serum CLOCK, BMAL1, PER1 and CRY1 protein expression levels were detected by Elisa. Pearson correlation analysis counted the relationship between SHR blood pressure rhythm and circadian clock protein fluctuation after ischemia-reperfusion. Results The proportion of abnormal blood pressure patterns in the SHR + tMCAO group was significantly higher than that in the SHR group, the serum CLOCK expression was relatively constant, and the circadian rhythm of BMAL1, PER1 and CRY1 protein expression changed significantly. Pearson analysis showed that PER1 protein level was negatively correlated with dipper (r = -0.565, P = 0.002) and extreme-dipper (r = -0.531, P = 0.001) blood pressure, and was significantly positively correlated with non-dipper blood pressure (r = 0.620, P < 0.001). Conclusion The rhythm pattern of blood pressure after ischemia-reperfusion in SHR is obviously disordered, and it is closely related to the regulation of Per1 gene.

scholarly journals Combination of Exercise Training and SOD Mimetic Tempol Enhances Upregulation of Nitric Oxide Synthase in the Kidney of Spontaneously Hypertensive Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Pengyu Cao ◽  
Osamu Ito ◽  
Daisuke Ito ◽  
Rong Rong ◽  
Yang Zheng ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Exercise Training ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Sod Activity ◽  
Spontaneously Hypertensive ◽  
Nadph Oxidase Activity ◽  
Expression Levels

Both exercise training (Ex) and superoxide dismutase (SOD) mimetic tempol have antihypertensive and renal protective effects in rodent models of several hypertensions. We recently reported that Ex increases nitric oxide (NO) production and the expression levels of endothelial and neuronal NO synthase (eNOS and nNOS) in the kidney and aorta of the spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto rats (WKY). We also found that endogenous hydrogen peroxide (H2O2) upregulates the expression levels of eNOS and nNOS in SHR. To elucidate the mechanism of the Ex-upregulated NO system in the kidney, we examined the additive effect of Ex and tempol on the renal NO system in SHR and WKY. Our data showed that, in SHR, both Ex and tempol increase the levels of H2O2 and nitrate/nitrite (NOx) in plasma and urine. We also observed an increased renal NOS activity and upregulated expression levels of eNOS and nNOS with decreased NADPH oxidase activity. The effects of the combination of Ex and tempol on these variables were cumulate in SHR. On the other hand, we found that Ex increases these variables with increased renal NADPH oxidase activity, but tempol did not change these variables or affect the Ex-induced upregulation in the activity and expression of NOS in WKY. The SOD activity in the kidney and aorta was activated by tempol only in SHR, but not in WKY; whereas Ex increased SOD activity only in the aorta in both SHR and WKY. These results indicate that Ex-induced endogenous H2O2 produced in the blood vessel and other organs outside of the kidney may be carried to the kidney by blood flow and stimulates the NO system in the kidney.

A Deficit in preprodynorphin mRNA expression in hippocampus of spontaneously hypertensive rats

1994 ◽  
Vol 23 (4) ◽  
pp. 345-348 ◽  
Author(s):  
John Q. Wang ◽  
Sijia Li ◽  
Alphonse J. Ingenito ◽  
Jacqueline F. McGinty
Keyword(s):  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

scholarly journals THE EFFECTS OF LEPTIN ADMINISTRATION ON RENAL FUNCTION IN SPONTANEOUSLY HYPERTENSIVE RATS

2016 ◽  
Vol 10 (1) ◽  
pp. 108
Author(s):  
Brinnell Annette Caszo ◽  
Azdayanti Muslim ◽  
Zanariah Awang ◽  
Effat Omar ◽  
Effendi Ibrahim ◽  
...  
Keyword(s):  
Renal Function ◽  
Growth Factor ◽  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Transforming Growth Factor ◽  
Morphological Changes ◽  
Transforming Growth Factor Β1 ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Tgf Β1

ABSTRACTObjective: Elevated levels of leptin may be responsible directly for progression and severity of renal disease in obesity and hypertension. It may exertits effects by promoting fibrosis through the actions of transforming growth factor-β1 (TGF-β1) and the Smad pathway. This study determines theeffect of leptin administration on the development of renal fibrosis in nonobese spontaneously hypertensive rats (SHRs).Methods: Male SHRs, aged 12-14 weeks, were injected with either leptin (60 µg/kg/day) or saline (for the control group) subcutaneously daily for42 days. At the end of the experimental period, animals were euthanized and their kidneys were removed. The right kidney was harvested for thedetermination of messenger ribonucleic acid (mRNA) expression of TGF-β1, Smad2, Smad3, and bone morphogenic protein 7 (BMP7). The left kidneyswere stored in neutral buffered 10% formalin until they were processed and stained with hematoxylin and eosin. Prepared slides were examinedunder light microscopy. 30 consecutive glomeruli were examined for the cell counts based on the number of nuclei seen and the total area of glomeruli.Results: No significant difference was evident in renal function between control and leptin-treated rats. Cellularity and area of glomeruli were also notdifferent between the two groups. mRNA expression of TGF-β1, Smad2, and BMP7 were, however, higher in leptin-treated rats.Conclusion: It appears that 6 weeks of leptin administration increases renal TGF-β1 and Smad2 levels but with little morphological changes in thekidney. Whether the elevated BMP7 expression was responsible for lack of effect of leptin on renal morphological changes remains unclear.Keywords: Leptin, Renal function, Hypertension, Glomerulus, Transforming growth factor-β1, Smad, Spontaneously hypertensive rats.

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