scholarly journals Is the ultimobranchial body a reality or myth: a study using serial sections of human embryos

2016 ◽  
Vol 93 (2) ◽  
pp. 29-40
Author(s):  
Yohei HONKURA ◽  
Masahito YAMAMOTO ◽  
Toshihito YOSHIMOTO ◽  
Jose Francisco RODRIGUEZ-VAZQUEZ ◽  
Gen MURAKAMI ◽  
...  
Keyword(s):  
Human Embryos ◽  
Serial Sections ◽  
Ultimobranchial Body

scholarly journals Median Sacral Artery, Sympathetic Nerves, and the Coccygeal Body: A Study Using Serial Sections of Human Embryos and Fetuses

2016 ◽  
Vol 299 (7) ◽  
pp. 819-827 ◽  
Author(s):  
Zhe Wu Jin ◽  
Kwang Ho Cho ◽  
Hyung Suk Jang ◽  
Gen Murakami ◽  
Jose Francisco Rodríguez-Vázquez
Keyword(s):  
Sympathetic Nerves ◽  
Human Embryos ◽  
Serial Sections ◽  
Median Sacral Artery ◽  
Human Embryos And Fetuses

Embryonic and Early Fetal Development of Human Lung Vasculature and Its Functional Implications

2000 ◽  
Vol 3 (5) ◽  
pp. 439-449 ◽  
Author(s):  
Daphne E. deMello ◽  
Lynne M. Reid
Keyword(s):  
Pulmonary Artery ◽  
Human Lung ◽  
Pulmonary Veins ◽  
Pulmonary Vasculature ◽  
Human Embryos ◽  
Vascular Structure ◽  
Serial Sections ◽  
Peripheral Airways ◽  
Functional Implications ◽  
Airway Branching

Recently, we have identified in the mouse three processes involved in the early development of pulmonary vasculature: angiogenesis for branching of central vessels, vasculogenesis (lakes in the mesenchyme) for peripheral vessels, and a lytic process to establish luminal connection between the two. We have established that these three processes also operate in the human by studying serial sections of human embryos and early fetuses. Vascular lakes of hematopoietic cells appear at stage 13, i.e., 4+ weeks gestational age (GA), the first intrapulmonary vascular structure to appear. At stage 20 (50.5 days GA), a venous network with luminal connections to central pulmonary veins (PV) is present. Airways have not yet reached these regions of lung.At its first intrapulmonary appearance, the pulmonary artery (PA) is small and thick walled: it runs with the airway but its branching is slower, so many peripheral airways are not accompanied by a PA branch. By contrast, the PV has a peripheral patent network well before the PA.In the pseudoglandular phase, airway branching continues, and the PA catches up so that small PA branches are found with all airways. Later in this phase small nonmuscular vessels lie in the mesenchyme close to airway epithelium.By the early canalicular phase and the age of viability, continuity between pulmonary artery and the peripheral capillary network must be established. In a 10-week fetus several structures suggesting a breakthrough site were seen. Air-blood barrier structure is first seen at 19 weeks. Thus in the lung, the PA and PV are dissociated in their timing and pattern of branching. Early veins are present diffusely through the mesenchyme and establish central luminal connection to the main pulmonary vein before airway or artery are present at this level.

Origin of the Ultimobranchial Body and Its Colonizing Cells in Human Embryos

1989 ◽  
Vol 136 (4) ◽  
pp. 325-330 ◽  
Author(s):  
J.A. Mérida-Velasco ◽  
J.D. García-García ◽  
J. Espín-Ferra ◽  
J. Linares
Keyword(s):  
Human Embryos ◽  
Ultimobranchial Body

DEVELOPMENT OF THE PULMONARY VEINS

PEDIATRICS ◽  
1956 ◽  
Vol 18 (6) ◽  
pp. 880-887
Author(s):  
Catherine A. Neill
Keyword(s):  
Pulmonary Vein ◽  
Pulmonary Veins ◽  
Venous Drainage ◽  
Atrial Septum ◽  
Sinus Venosus ◽  
Human Embryos ◽  
Left Auricle ◽  
Serial Sections ◽  
The Common ◽  
Common Pulmonary Vein

The examination of serial sections of human embryos between 24 and 34 days (3 to 11 mm) and the use of plastic reconstructions, showed that the common pulmonary vein develops as an outgrowth from the medial superior wall of the left auricle and unites with the angioblastic plexus of the developing lung bud. No evidence was found that the vein connects directly with the sinus venosus in the early stages, and later shifts in position as the atrial septum grows. Anomalous pulmonary venous drainage is classified in four main types, and theories of development are briefly discussed.

scholarly journals Persistent right umbilical vein: a study using serial sections of human embryos and fetuses

2018 ◽  
Vol 51 (3) ◽  
pp. 218 ◽  
Author(s):  
Ji Hyun Kim ◽  
Zhe Wu Jin ◽  
Gen Murakami ◽  
Ok Hee Chai ◽  
José Francisco Rodríguez-Vázquez
Keyword(s):  
Umbilical Vein ◽  
Human Embryos ◽  
Serial Sections ◽  
Human Embryos And Fetuses

scholarly journals Development of sacral vertebrae in human embryos and fetuses

2020 ◽  
Vol 9 (3) ◽  
pp. 22-27
Author(s):  
S. L. Kabak ◽  
V. V. Zatochnaya
Keyword(s):  
Spinal Column ◽  
Human Embryos ◽  
Serial Sections ◽  
Lumbosacral Region ◽  
Intrauterine Development ◽  
Alizarin Red ◽  
Fetal Spine ◽  
Human Embryos And Fetuses ◽  
Hematoxylin And Eosin ◽  
Medical University

The aim of the study is to define the features of organogenesis and histogenesis of sacral vertebrae in human embryos, which predetermine the anatomical structure of the sacrum in postnatal ontogenesis. Material and methods. The authors studied human embryo serial sections at 17–23 stages from the Carnegie virtual embryological collection, total preparations and serial sections of the lumbosacral region of the fetal spine at 8–20 weeks of gestation without visible developmental anomalies. Histological sections were stained with hematoxylin and eosin; total preparations were stained with alizarin red and alcian blue. In addition, the study included 19 adult sacra from the anatomical collection of the Belarusian State Medical University. Results. It was found that consolidation of the anlages of the upper sacral vertebrae starts at the early stages of embryogenesis by the fusion of their costal-transverse processes. During the first half of intrauterine development, the arch and articular processes of the adjacent vertebrae remain separate from each other. In contrast to other segments of the spinal column, five primary centers of ossification are formed in the anlages of the upper sacral vertebrae

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

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